Experiment: MoLS4 with L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVUA0072 and DVUA0073 are separated by 108 nucleotides DVUA0073 and DVUA0074 are separated by 90 nucleotides DVUA0074 and DVUA0075 are separated by 87 nucleotides
DVUA0072: DVUA0072 - glycosyl transferase, group 1 family protein (TIGR), at 96,400 to 97,737
DVUA0072
DVUA0073: DVUA0073 - asparagine synthase (glutamine-hydrolyzing), putative (TIGR), at 97,846 to 100,515
DVUA0073
DVUA0074: DVUA0074 - sulfotransferase family protein (TIGR), at 100,606 to 101,295
DVUA0074
DVUA0075: DVUA0075 - radical SAM domain protein (TIGR), at 101,383 to 102,789
DVUA0075
Position (kb) 97
98
99
100
101 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 97.825 kb on + strand at 97.833 kb on - strand at 97.894 kb on - strand at 99.081 kb on + strand, within DVUA0073 at 99.092 kb on + strand, within DVUA0073 at 100.716 kb on + strand, within DVUA0074 at 100.764 kb on - strand, within DVUA0074 at 100.813 kb on - strand, within DVUA0074 at 100.852 kb on + strand, within DVUA0074 at 100.888 kb on - strand, within DVUA0074 at 100.891 kb on + strand, within DVUA0074 at 100.899 kb on - strand, within DVUA0074 at 101.037 kb on - strand, within DVUA0074 at 101.043 kb on - strand, within DVUA0074 at 101.269 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Alanine (N) remove 97,825 + +0.6 97,833 - +1.2 97,894 - -2.8 99,081 + DVUA0073 0.46 -0.7 99,092 + DVUA0073 0.47 +1.2 100,716 + DVUA0074 0.16 +0.7 100,764 - DVUA0074 0.23 -1.1 100,813 - DVUA0074 0.30 +0.2 100,852 + DVUA0074 0.36 -0.9 100,888 - DVUA0074 0.41 -1.3 100,891 + DVUA0074 0.41 -0.5 100,899 - DVUA0074 0.42 -1.0 101,037 - DVUA0074 0.62 -1.3 101,043 - DVUA0074 0.63 +0.6 101,269 + +1.2
Or see this region's nucleotide sequence