Experiment: MoLS4 with L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU3026 and glcD are separated by 174 nucleotides glcD and DVU3028 are separated by 31 nucleotides
DVU3026: DVU3026 - L-lactate permease family protein (TIGR), at 3,147,520 to 3,149,232
DVU3026
DVU3027: glcD - glycolate oxidase, subunit GlcD (TIGR), at 3,149,407 to 3,150,792
glcD
DVU3028: DVU3028 - iron-sulfur cluster-binding protein (TIGR), at 3,150,824 to 3,152,092
DVU3028
Position (kb)
3149
3150
3151 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3148.485 kb on + strand, within DVU3026 at 3148.657 kb on + strand, within DVU3026 at 3148.746 kb on + strand, within DVU3026 at 3148.770 kb on + strand, within DVU3026 at 3148.772 kb on + strand, within DVU3026 at 3148.778 kb on - strand, within DVU3026 at 3148.833 kb on - strand, within DVU3026 at 3148.938 kb on + strand, within DVU3026 at 3148.974 kb on - strand, within DVU3026 at 3149.136 kb on + strand at 3149.147 kb on + strand at 3149.155 kb on - strand at 3149.179 kb on - strand at 3149.226 kb on + strand at 3149.255 kb on - strand at 3149.533 kb on + strand at 3149.601 kb on + strand, within glcD at 3149.809 kb on + strand, within glcD at 3150.028 kb on + strand, within glcD at 3150.047 kb on - strand, within glcD at 3150.364 kb on - strand, within glcD at 3150.413 kb on + strand, within glcD at 3150.516 kb on - strand, within glcD at 3150.661 kb on + strand at 3150.678 kb on + strand at 3150.686 kb on - strand at 3150.882 kb on - strand at 3150.905 kb on + strand at 3151.096 kb on + strand, within DVU3028 at 3151.152 kb on + strand, within DVU3028 at 3151.152 kb on + strand, within DVU3028 at 3151.152 kb on + strand, within DVU3028 at 3151.160 kb on - strand, within DVU3028 at 3151.170 kb on - strand, within DVU3028 at 3151.217 kb on - strand, within DVU3028 at 3151.217 kb on - strand, within DVU3028 at 3151.240 kb on - strand, within DVU3028 at 3151.350 kb on + strand, within DVU3028 at 3151.424 kb on + strand, within DVU3028 at 3151.555 kb on + strand, within DVU3028 at 3151.582 kb on + strand, within DVU3028 at 3151.656 kb on + strand, within DVU3028 at 3151.670 kb on + strand, within DVU3028 at 3151.681 kb on + strand, within DVU3028 at 3151.725 kb on - strand, within DVU3028 at 3151.761 kb on - strand, within DVU3028
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Alanine (N) remove 3,148,485 + DVU3026 0.56 +0.7 3,148,657 + DVU3026 0.66 +0.2 3,148,746 + DVU3026 0.72 +0.5 3,148,770 + DVU3026 0.73 +0.0 3,148,772 + DVU3026 0.73 +1.0 3,148,778 - DVU3026 0.73 +0.8 3,148,833 - DVU3026 0.77 -0.0 3,148,938 + DVU3026 0.83 -0.5 3,148,974 - DVU3026 0.85 +0.2 3,149,136 + -0.6 3,149,147 + +0.4 3,149,155 - +1.3 3,149,179 - -0.2 3,149,226 + +0.2 3,149,255 - -1.1 3,149,533 + +0.8 3,149,601 + glcD DVU3027 0.14 +1.0 3,149,809 + glcD DVU3027 0.29 +0.6 3,150,028 + glcD DVU3027 0.45 +0.0 3,150,047 - glcD DVU3027 0.46 +2.5 3,150,364 - glcD DVU3027 0.69 +1.3 3,150,413 + glcD DVU3027 0.73 +1.5 3,150,516 - glcD DVU3027 0.80 +0.8 3,150,661 + +0.3 3,150,678 + +1.9 3,150,686 - +2.6 3,150,882 - +1.9 3,150,905 + +0.6 3,151,096 + DVU3028 0.21 +0.9 3,151,152 + DVU3028 0.26 +1.5 3,151,152 + DVU3028 0.26 +0.9 3,151,152 + DVU3028 0.26 +3.2 3,151,160 - DVU3028 0.26 -1.4 3,151,170 - DVU3028 0.27 +1.1 3,151,217 - DVU3028 0.31 +1.3 3,151,217 - DVU3028 0.31 +1.4 3,151,240 - DVU3028 0.33 +1.2 3,151,350 + DVU3028 0.41 +0.1 3,151,424 + DVU3028 0.47 +0.7 3,151,555 + DVU3028 0.58 +2.0 3,151,582 + DVU3028 0.60 +0.2 3,151,656 + DVU3028 0.66 +0.5 3,151,670 + DVU3028 0.67 +0.3 3,151,681 + DVU3028 0.68 +0.3 3,151,725 - DVU3028 0.71 +1.2 3,151,761 - DVU3028 0.74 +0.0
Or see this region's nucleotide sequence