Experiment: MoLS4 with L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2294 and DVU2295 are separated by 506 nucleotides DVU2295 and mtgA are separated by 427 nucleotides
DVU2294: DVU2294 - femAB family protein (TIGR), at 2,385,828 to 2,386,907
DVU2294
DVU2295: DVU2295 - methyl-accepting chemotaxis protein (TIGR), at 2,387,414 to 2,389,744
DVU2295
DVU2296: mtgA - monofunctional biosynthetic peptidoglycan transglycosylase (TIGR), at 2,390,172 to 2,390,918
mtgA
Position (kb)
2387
2388
2389
2390 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2386.500 kb on - strand, within DVU2294 at 2386.557 kb on - strand, within DVU2294 at 2386.630 kb on - strand, within DVU2294 at 2386.675 kb on + strand, within DVU2294 at 2386.675 kb on + strand, within DVU2294 at 2386.675 kb on + strand, within DVU2294 at 2386.697 kb on - strand, within DVU2294 at 2386.700 kb on + strand, within DVU2294 at 2386.708 kb on - strand, within DVU2294 at 2386.708 kb on - strand, within DVU2294 at 2386.808 kb on - strand at 2386.822 kb on + strand at 2386.847 kb on - strand at 2386.854 kb on - strand at 2386.994 kb on + strand at 2387.066 kb on + strand at 2387.283 kb on - strand at 2387.290 kb on + strand at 2387.413 kb on + strand at 2387.523 kb on + strand at 2387.637 kb on + strand at 2387.678 kb on + strand, within DVU2295 at 2387.679 kb on - strand, within DVU2295 at 2387.783 kb on - strand, within DVU2295 at 2387.953 kb on + strand, within DVU2295 at 2387.960 kb on + strand, within DVU2295 at 2387.969 kb on - strand, within DVU2295 at 2388.014 kb on + strand, within DVU2295 at 2388.014 kb on + strand, within DVU2295 at 2388.234 kb on - strand, within DVU2295 at 2388.285 kb on + strand, within DVU2295 at 2388.381 kb on + strand, within DVU2295 at 2388.449 kb on - strand, within DVU2295 at 2388.603 kb on + strand, within DVU2295 at 2388.647 kb on + strand, within DVU2295 at 2388.655 kb on - strand, within DVU2295 at 2388.677 kb on - strand, within DVU2295 at 2388.688 kb on - strand, within DVU2295 at 2388.690 kb on + strand, within DVU2295 at 2388.735 kb on + strand, within DVU2295 at 2388.743 kb on - strand, within DVU2295 at 2388.773 kb on + strand, within DVU2295 at 2388.853 kb on - strand, within DVU2295 at 2388.939 kb on + strand, within DVU2295 at 2389.061 kb on - strand, within DVU2295 at 2389.093 kb on - strand, within DVU2295 at 2389.142 kb on + strand, within DVU2295 at 2389.142 kb on + strand, within DVU2295 at 2389.176 kb on + strand, within DVU2295 at 2389.189 kb on - strand, within DVU2295 at 2389.204 kb on - strand, within DVU2295 at 2389.243 kb on + strand, within DVU2295 at 2389.243 kb on + strand, within DVU2295 at 2389.266 kb on - strand, within DVU2295 at 2389.309 kb on - strand, within DVU2295 at 2389.346 kb on + strand, within DVU2295 at 2389.365 kb on + strand, within DVU2295 at 2389.403 kb on + strand, within DVU2295 at 2389.403 kb on + strand, within DVU2295 at 2389.411 kb on - strand, within DVU2295 at 2389.411 kb on - strand, within DVU2295 at 2389.445 kb on - strand, within DVU2295 at 2389.467 kb on - strand, within DVU2295 at 2389.573 kb on + strand at 2389.660 kb on - strand at 2389.881 kb on + strand at 2390.011 kb on - strand at 2390.052 kb on + strand at 2390.125 kb on - strand at 2390.138 kb on - strand at 2390.283 kb on + strand, within mtgA at 2390.283 kb on + strand, within mtgA at 2390.293 kb on + strand, within mtgA at 2390.306 kb on - strand, within mtgA at 2390.373 kb on + strand, within mtgA at 2390.373 kb on + strand, within mtgA at 2390.425 kb on + strand, within mtgA at 2390.437 kb on - strand, within mtgA at 2390.464 kb on - strand, within mtgA at 2390.569 kb on - strand, within mtgA at 2390.612 kb on + strand, within mtgA at 2390.679 kb on - strand, within mtgA at 2390.686 kb on + strand, within mtgA
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Alanine (N) remove 2,386,500 - DVU2294 0.62 -0.2 2,386,557 - DVU2294 0.68 +0.3 2,386,630 - DVU2294 0.74 -0.4 2,386,675 + DVU2294 0.78 +0.1 2,386,675 + DVU2294 0.78 -0.0 2,386,675 + DVU2294 0.78 +0.0 2,386,697 - DVU2294 0.80 -1.1 2,386,700 + DVU2294 0.81 -0.0 2,386,708 - DVU2294 0.81 -0.9 2,386,708 - DVU2294 0.81 +1.2 2,386,808 - -0.6 2,386,822 + +0.1 2,386,847 - +0.1 2,386,854 - -0.3 2,386,994 + +0.0 2,387,066 + -0.0 2,387,283 - -1.2 2,387,290 + +0.8 2,387,413 + -0.3 2,387,523 + -0.7 2,387,637 + -0.2 2,387,678 + DVU2295 0.11 -3.8 2,387,679 - DVU2295 0.11 -0.6 2,387,783 - DVU2295 0.16 -0.8 2,387,953 + DVU2295 0.23 +0.4 2,387,960 + DVU2295 0.23 -1.6 2,387,969 - DVU2295 0.24 -0.5 2,388,014 + DVU2295 0.26 -0.1 2,388,014 + DVU2295 0.26 -1.1 2,388,234 - DVU2295 0.35 -0.0 2,388,285 + DVU2295 0.37 -0.7 2,388,381 + DVU2295 0.41 -2.8 2,388,449 - DVU2295 0.44 +0.3 2,388,603 + DVU2295 0.51 -1.1 2,388,647 + DVU2295 0.53 +1.4 2,388,655 - DVU2295 0.53 -0.5 2,388,677 - DVU2295 0.54 -0.8 2,388,688 - DVU2295 0.55 -0.9 2,388,690 + DVU2295 0.55 +0.3 2,388,735 + DVU2295 0.57 -0.5 2,388,743 - DVU2295 0.57 -0.2 2,388,773 + DVU2295 0.58 +0.1 2,388,853 - DVU2295 0.62 +0.4 2,388,939 + DVU2295 0.65 +0.1 2,389,061 - DVU2295 0.71 +1.4 2,389,093 - DVU2295 0.72 -1.6 2,389,142 + DVU2295 0.74 -0.3 2,389,142 + DVU2295 0.74 -0.3 2,389,176 + DVU2295 0.76 -0.2 2,389,189 - DVU2295 0.76 +0.5 2,389,204 - DVU2295 0.77 -0.5 2,389,243 + DVU2295 0.78 -0.6 2,389,243 + DVU2295 0.78 -0.2 2,389,266 - DVU2295 0.79 -0.8 2,389,309 - DVU2295 0.81 +0.3 2,389,346 + DVU2295 0.83 -1.0 2,389,365 + DVU2295 0.84 -0.6 2,389,403 + DVU2295 0.85 +0.4 2,389,403 + DVU2295 0.85 -0.2 2,389,411 - DVU2295 0.86 -0.4 2,389,411 - DVU2295 0.86 +0.1 2,389,445 - DVU2295 0.87 -0.9 2,389,467 - DVU2295 0.88 -0.6 2,389,573 + -0.8 2,389,660 - -0.5 2,389,881 + +1.0 2,390,011 - -0.8 2,390,052 + +1.5 2,390,125 - +0.9 2,390,138 - -2.1 2,390,283 + mtgA DVU2296 0.15 +1.1 2,390,283 + mtgA DVU2296 0.15 +0.1 2,390,293 + mtgA DVU2296 0.16 +0.1 2,390,306 - mtgA DVU2296 0.18 -0.6 2,390,373 + mtgA DVU2296 0.27 +0.3 2,390,373 + mtgA DVU2296 0.27 -0.0 2,390,425 + mtgA DVU2296 0.34 +1.1 2,390,437 - mtgA DVU2296 0.35 -1.6 2,390,464 - mtgA DVU2296 0.39 +2.4 2,390,569 - mtgA DVU2296 0.53 -0.4 2,390,612 + mtgA DVU2296 0.59 -1.7 2,390,679 - mtgA DVU2296 0.68 -0.1 2,390,686 + mtgA DVU2296 0.69 -0.4
Or see this region's nucleotide sequence