Experiment: H2 N-free with 10mM L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01180 and MMP_RS01185 are separated by 115 nucleotides MMP_RS01185 and MMP_RS01190 are separated by 22 nucleotides MMP_RS01190 and MMP_RS01195 are separated by 2 nucleotides MMP_RS01195 and MMP_RS01200 are separated by 120 nucleotides
MMP_RS01180: MMP_RS01180 - XTP/dITP diphosphatase, at 221,545 to 222,096
_RS01180
MMP_RS01185: MMP_RS01185 - hypothetical protein, at 222,212 to 222,622
_RS01185
MMP_RS01190: MMP_RS01190 - cadmium-translocating P-type ATPase, at 222,645 to 224,720
_RS01190
MMP_RS01195: MMP_RS01195 - metalloregulator ArsR/SmtB family transcription factor, at 224,723 to 225,067
_RS01195
MMP_RS01200: MMP_RS01200 - hypothetical protein, at 225,188 to 225,925
_RS01200
Position (kb) 222
223
224
225 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 221.759 kb on - strand, within MMP_RS01180 at 221.915 kb on + strand, within MMP_RS01180 at 222.063 kb on - strand at 222.086 kb on - strand at 222.097 kb on + strand at 222.134 kb on - strand at 222.135 kb on + strand at 222.142 kb on + strand at 222.158 kb on - strand at 222.158 kb on - strand at 222.158 kb on - strand at 222.159 kb on + strand at 222.171 kb on + strand at 222.186 kb on + strand at 222.188 kb on - strand at 222.444 kb on - strand, within MMP_RS01185 at 222.444 kb on - strand, within MMP_RS01185 at 222.447 kb on + strand, within MMP_RS01185 at 222.449 kb on + strand, within MMP_RS01185 at 222.475 kb on - strand, within MMP_RS01185 at 222.551 kb on - strand, within MMP_RS01185 at 222.551 kb on - strand, within MMP_RS01185 at 222.552 kb on + strand, within MMP_RS01185 at 222.552 kb on + strand, within MMP_RS01185 at 222.553 kb on - strand, within MMP_RS01185 at 222.576 kb on + strand, within MMP_RS01185 at 222.607 kb on + strand at 223.052 kb on + strand, within MMP_RS01190 at 223.196 kb on - strand, within MMP_RS01190 at 223.197 kb on + strand, within MMP_RS01190 at 223.586 kb on + strand, within MMP_RS01190 at 223.776 kb on - strand, within MMP_RS01190 at 223.776 kb on - strand, within MMP_RS01190 at 223.841 kb on - strand, within MMP_RS01190 at 224.265 kb on - strand, within MMP_RS01190 at 224.349 kb on + strand, within MMP_RS01190 at 224.367 kb on - strand, within MMP_RS01190 at 224.370 kb on - strand, within MMP_RS01190 at 224.374 kb on - strand, within MMP_RS01190 at 224.485 kb on - strand, within MMP_RS01190 at 224.529 kb on - strand at 224.599 kb on + strand at 224.725 kb on + strand at 224.966 kb on + strand, within MMP_RS01195 at 225.125 kb on + strand at 225.187 kb on - strand at 225.187 kb on - strand at 225.188 kb on + strand at 225.412 kb on + strand, within MMP_RS01200 at 225.412 kb on + strand, within MMP_RS01200 at 225.572 kb on + strand, within MMP_RS01200 at 225.596 kb on + strand, within MMP_RS01200 at 225.693 kb on - strand, within MMP_RS01200
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Histidine remove 221,759 - MMP_RS01180 0.39 -0.4 221,915 + MMP_RS01180 0.67 -1.0 222,063 - -0.3 222,086 - +0.3 222,097 + -0.7 222,134 - +0.4 222,135 + -1.0 222,142 + -0.5 222,158 - +0.4 222,158 - -0.0 222,158 - +1.0 222,159 + -0.1 222,171 + +1.0 222,186 + -0.5 222,188 - +0.4 222,444 - MMP_RS01185 0.56 -0.2 222,444 - MMP_RS01185 0.56 +0.2 222,447 + MMP_RS01185 0.57 +0.8 222,449 + MMP_RS01185 0.58 -0.2 222,475 - MMP_RS01185 0.64 -0.8 222,551 - MMP_RS01185 0.82 +0.3 222,551 - MMP_RS01185 0.82 +0.4 222,552 + MMP_RS01185 0.83 -0.9 222,552 + MMP_RS01185 0.83 +0.7 222,553 - MMP_RS01185 0.83 +0.1 222,576 + MMP_RS01185 0.89 -0.0 222,607 + +0.3 223,052 + MMP_RS01190 0.20 -2.3 223,196 - MMP_RS01190 0.27 -1.4 223,197 + MMP_RS01190 0.27 -2.7 223,586 + MMP_RS01190 0.45 -2.6 223,776 - MMP_RS01190 0.54 -3.1 223,776 - MMP_RS01190 0.54 -0.8 223,841 - MMP_RS01190 0.58 -2.7 224,265 - MMP_RS01190 0.78 -4.6 224,349 + MMP_RS01190 0.82 -3.5 224,367 - MMP_RS01190 0.83 -1.9 224,370 - MMP_RS01190 0.83 -2.3 224,374 - MMP_RS01190 0.83 -3.9 224,485 - MMP_RS01190 0.89 -2.2 224,529 - -2.8 224,599 + -2.8 224,725 + -3.2 224,966 + MMP_RS01195 0.70 -1.3 225,125 + +0.4 225,187 - +1.1 225,187 - +0.8 225,188 + -0.3 225,412 + MMP_RS01200 0.30 -0.1 225,412 + MMP_RS01200 0.30 +0.5 225,572 + MMP_RS01200 0.52 +0.1 225,596 + MMP_RS01200 0.55 +0.3 225,693 - MMP_RS01200 0.68 +1.1
Or see this region's nucleotide sequence