Experiment: m.b. Gentamicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c09050 and PGA1_c09070 are separated by 23 nucleotides PGA1_c09070 and PGA1_c09090 are separated by 3 nucleotides PGA1_c09090 and PGA1_c09100 overlap by 32 nucleotides PGA1_c09100 and PGA1_c09110 overlap by 4 nucleotides
PGA1_c09050: PGA1_c09050 - thiamine-phosphate pyrophosphorylase ThiE, at 932,960 to 933,553
_c09050
PGA1_c09070: PGA1_c09070 - thiazole biosynthesis protein ThiG, at 933,577 to 934,344
_c09070
PGA1_c09090: PGA1_c09090 - thiamine biosynthesis protein ThiS, at 934,348 to 934,545
_c09090
PGA1_c09100: PGA1_c09100 - putative thiamine biosynthesis oxidoreductase ThiO, at 934,514 to 935,488
_c09100
PGA1_c09110: PGA1_c09110 - thiamine biosynthesis protein ThiC, at 935,485 to 937,284
_c09110
Position (kb)
934
935
936 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 933.518 kb on + strand at 933.575 kb on + strand at 933.580 kb on + strand at 933.581 kb on - strand at 933.583 kb on - strand at 933.583 kb on - strand at 933.583 kb on - strand at 933.583 kb on - strand at 933.603 kb on - strand at 933.689 kb on + strand, within PGA1_c09070 at 933.702 kb on - strand, within PGA1_c09070 at 933.731 kb on + strand, within PGA1_c09070 at 933.731 kb on + strand, within PGA1_c09070 at 933.739 kb on - strand, within PGA1_c09070 at 933.767 kb on + strand, within PGA1_c09070 at 933.862 kb on + strand, within PGA1_c09070 at 933.865 kb on - strand, within PGA1_c09070 at 933.878 kb on + strand, within PGA1_c09070 at 933.881 kb on - strand, within PGA1_c09070 at 933.901 kb on - strand, within PGA1_c09070 at 933.948 kb on + strand, within PGA1_c09070 at 933.972 kb on + strand, within PGA1_c09070 at 933.978 kb on - strand, within PGA1_c09070 at 933.980 kb on - strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.076 kb on + strand, within PGA1_c09070 at 934.084 kb on - strand, within PGA1_c09070 at 934.103 kb on - strand, within PGA1_c09070 at 934.103 kb on - strand, within PGA1_c09070 at 934.108 kb on + strand, within PGA1_c09070 at 934.142 kb on + strand, within PGA1_c09070 at 934.142 kb on + strand, within PGA1_c09070 at 934.150 kb on - strand, within PGA1_c09070 at 934.152 kb on + strand, within PGA1_c09070 at 934.152 kb on + strand, within PGA1_c09070 at 934.162 kb on + strand, within PGA1_c09070 at 934.275 kb on + strand at 934.275 kb on - strand at 934.306 kb on - strand at 934.311 kb on + strand at 934.311 kb on + strand at 934.311 kb on + strand at 934.319 kb on - strand at 934.339 kb on - strand at 934.354 kb on - strand at 934.364 kb on + strand at 934.472 kb on - strand, within PGA1_c09090 at 934.513 kb on - strand, within PGA1_c09090 at 934.529 kb on - strand at 934.539 kb on + strand at 934.568 kb on - strand at 934.624 kb on - strand, within PGA1_c09100 at 934.636 kb on - strand, within PGA1_c09100 at 934.659 kb on - strand, within PGA1_c09100 at 934.659 kb on - strand, within PGA1_c09100 at 934.685 kb on + strand, within PGA1_c09100 at 934.685 kb on + strand, within PGA1_c09100 at 934.693 kb on - strand, within PGA1_c09100 at 934.693 kb on - strand, within PGA1_c09100 at 934.693 kb on - strand, within PGA1_c09100 at 934.710 kb on + strand, within PGA1_c09100 at 934.719 kb on - strand, within PGA1_c09100 at 934.846 kb on + strand, within PGA1_c09100 at 934.854 kb on - strand, within PGA1_c09100 at 934.854 kb on - strand, within PGA1_c09100 at 934.903 kb on + strand, within PGA1_c09100 at 934.943 kb on + strand, within PGA1_c09100 at 935.068 kb on - strand, within PGA1_c09100 at 935.081 kb on + strand, within PGA1_c09100 at 935.089 kb on - strand, within PGA1_c09100 at 935.139 kb on + strand, within PGA1_c09100 at 935.147 kb on - strand, within PGA1_c09100 at 935.147 kb on - strand, within PGA1_c09100 at 935.172 kb on + strand, within PGA1_c09100 at 935.177 kb on + strand, within PGA1_c09100 at 935.217 kb on - strand, within PGA1_c09100 at 935.228 kb on - strand, within PGA1_c09100 at 935.276 kb on - strand, within PGA1_c09100 at 935.351 kb on + strand, within PGA1_c09100 at 935.429 kb on - strand at 935.495 kb on + strand at 935.507 kb on + strand at 935.515 kb on - strand at 935.515 kb on - strand at 935.608 kb on + strand at 935.651 kb on - strand at 935.654 kb on + strand at 935.664 kb on + strand at 935.664 kb on + strand at 935.666 kb on + strand, within PGA1_c09110 at 935.682 kb on + strand, within PGA1_c09110 at 935.713 kb on + strand, within PGA1_c09110 at 935.779 kb on + strand, within PGA1_c09110 at 935.779 kb on + strand, within PGA1_c09110 at 935.786 kb on + strand, within PGA1_c09110 at 935.794 kb on - strand, within PGA1_c09110 at 935.950 kb on + strand, within PGA1_c09110 at 935.950 kb on + strand, within PGA1_c09110 at 935.964 kb on - strand, within PGA1_c09110 at 936.044 kb on + strand, within PGA1_c09110 at 936.076 kb on + strand, within PGA1_c09110 at 936.101 kb on - strand, within PGA1_c09110 at 936.107 kb on + strand, within PGA1_c09110 at 936.107 kb on + strand, within PGA1_c09110 at 936.126 kb on + strand, within PGA1_c09110 at 936.142 kb on + strand, within PGA1_c09110 at 936.181 kb on + strand, within PGA1_c09110 at 936.236 kb on - strand, within PGA1_c09110 at 936.349 kb on - strand, within PGA1_c09110 at 936.355 kb on + strand, within PGA1_c09110 at 936.380 kb on - strand, within PGA1_c09110 at 936.407 kb on + strand, within PGA1_c09110 at 936.440 kb on - strand, within PGA1_c09110 at 936.447 kb on - strand, within PGA1_c09110
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Gentamicin 0.004 mg/ml remove 933,518 + +0.6 933,575 + -0.1 933,580 + -0.0 933,581 - -1.2 933,583 - -0.3 933,583 - +0.7 933,583 - -0.1 933,583 - +0.3 933,603 - +1.2 933,689 + PGA1_c09070 0.15 +0.9 933,702 - PGA1_c09070 0.16 +0.7 933,731 + PGA1_c09070 0.20 +0.2 933,731 + PGA1_c09070 0.20 +0.1 933,739 - PGA1_c09070 0.21 +1.9 933,767 + PGA1_c09070 0.25 +0.4 933,862 + PGA1_c09070 0.37 +0.1 933,865 - PGA1_c09070 0.38 +1.1 933,878 + PGA1_c09070 0.39 +0.4 933,881 - PGA1_c09070 0.40 -0.4 933,901 - PGA1_c09070 0.42 +1.0 933,948 + PGA1_c09070 0.48 +0.5 933,972 + PGA1_c09070 0.51 +0.3 933,978 - PGA1_c09070 0.52 +1.1 933,980 - PGA1_c09070 0.52 +2.4 934,004 + PGA1_c09070 0.56 +0.2 934,004 + PGA1_c09070 0.56 +0.2 934,004 + PGA1_c09070 0.56 +1.5 934,004 + PGA1_c09070 0.56 +0.7 934,004 + PGA1_c09070 0.56 +0.9 934,004 + PGA1_c09070 0.56 +1.0 934,004 + PGA1_c09070 0.56 +0.2 934,004 + PGA1_c09070 0.56 +0.4 934,012 - PGA1_c09070 0.57 +1.3 934,012 - PGA1_c09070 0.57 -1.3 934,012 - PGA1_c09070 0.57 +1.1 934,012 - PGA1_c09070 0.57 -0.0 934,012 - PGA1_c09070 0.57 +0.7 934,012 - PGA1_c09070 0.57 +1.1 934,012 - PGA1_c09070 0.57 +0.8 934,012 - PGA1_c09070 0.57 +1.9 934,012 - PGA1_c09070 0.57 +1.2 934,076 + PGA1_c09070 0.65 -0.1 934,084 - PGA1_c09070 0.66 +1.3 934,103 - PGA1_c09070 0.68 +1.3 934,103 - PGA1_c09070 0.68 +1.7 934,108 + PGA1_c09070 0.69 +1.1 934,142 + PGA1_c09070 0.74 +0.8 934,142 + PGA1_c09070 0.74 +1.2 934,150 - PGA1_c09070 0.75 +1.8 934,152 + PGA1_c09070 0.75 +1.2 934,152 + PGA1_c09070 0.75 +0.5 934,162 + PGA1_c09070 0.76 -0.6 934,275 + -0.1 934,275 - +0.2 934,306 - +0.4 934,311 + +0.8 934,311 + +0.1 934,311 + +0.0 934,319 - +1.8 934,339 - +1.0 934,354 - -2.0 934,364 + +0.2 934,472 - PGA1_c09090 0.63 +1.3 934,513 - PGA1_c09090 0.83 +1.3 934,529 - +0.6 934,539 + +1.3 934,568 - +0.7 934,624 - PGA1_c09100 0.11 +1.0 934,636 - PGA1_c09100 0.13 +1.2 934,659 - PGA1_c09100 0.15 +1.4 934,659 - PGA1_c09100 0.15 +2.0 934,685 + PGA1_c09100 0.18 +0.8 934,685 + PGA1_c09100 0.18 +1.1 934,693 - PGA1_c09100 0.18 +0.7 934,693 - PGA1_c09100 0.18 +2.0 934,693 - PGA1_c09100 0.18 +0.5 934,710 + PGA1_c09100 0.20 -0.0 934,719 - PGA1_c09100 0.21 -0.3 934,846 + PGA1_c09100 0.34 +0.0 934,854 - PGA1_c09100 0.35 +1.5 934,854 - PGA1_c09100 0.35 +2.4 934,903 + PGA1_c09100 0.40 +0.8 934,943 + PGA1_c09100 0.44 +1.2 935,068 - PGA1_c09100 0.57 -0.2 935,081 + PGA1_c09100 0.58 -1.1 935,089 - PGA1_c09100 0.59 +1.5 935,139 + PGA1_c09100 0.64 +0.8 935,147 - PGA1_c09100 0.65 +0.2 935,147 - PGA1_c09100 0.65 +1.7 935,172 + PGA1_c09100 0.67 +1.4 935,177 + PGA1_c09100 0.68 +0.9 935,217 - PGA1_c09100 0.72 +1.5 935,228 - PGA1_c09100 0.73 +1.1 935,276 - PGA1_c09100 0.78 +0.5 935,351 + PGA1_c09100 0.86 +1.1 935,429 - +1.8 935,495 + +0.7 935,507 + +0.4 935,515 - +1.8 935,515 - +2.3 935,608 + +1.0 935,651 - +1.0 935,654 + +1.1 935,664 + +0.7 935,664 + -0.6 935,666 + PGA1_c09110 0.10 +1.0 935,682 + PGA1_c09110 0.11 +1.6 935,713 + PGA1_c09110 0.13 +0.7 935,779 + PGA1_c09110 0.16 +1.0 935,779 + PGA1_c09110 0.16 +0.1 935,786 + PGA1_c09110 0.17 +0.7 935,794 - PGA1_c09110 0.17 +0.5 935,950 + PGA1_c09110 0.26 +0.4 935,950 + PGA1_c09110 0.26 -1.8 935,964 - PGA1_c09110 0.27 +1.3 936,044 + PGA1_c09110 0.31 +1.9 936,076 + PGA1_c09110 0.33 +0.5 936,101 - PGA1_c09110 0.34 +2.3 936,107 + PGA1_c09110 0.35 +0.8 936,107 + PGA1_c09110 0.35 +1.6 936,126 + PGA1_c09110 0.36 +1.0 936,142 + PGA1_c09110 0.36 +0.7 936,181 + PGA1_c09110 0.39 -0.1 936,236 - PGA1_c09110 0.42 +1.8 936,349 - PGA1_c09110 0.48 +1.0 936,355 + PGA1_c09110 0.48 +0.6 936,380 - PGA1_c09110 0.50 +1.5 936,407 + PGA1_c09110 0.51 +1.0 936,440 - PGA1_c09110 0.53 +1.2 936,447 - PGA1_c09110 0.53 +1.3
Or see this region's nucleotide sequence