Experiment: m.b. Gentamicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c09030 and PGA1_c09040 overlap by 4 nucleotides PGA1_c09040 and PGA1_c09050 overlap by 25 nucleotides PGA1_c09050 and PGA1_c09070 are separated by 23 nucleotides
PGA1_c09030: PGA1_c09030 - phosphomethylpyrimidine kinase ThiD, at 931,201 to 931,998
_c09030
PGA1_c09040: PGA1_c09040 - putative adenylyltransferase ThiF, at 931,995 to 932,984
_c09040
PGA1_c09050: PGA1_c09050 - thiamine-phosphate pyrophosphorylase ThiE, at 932,960 to 933,553
_c09050
PGA1_c09070: PGA1_c09070 - thiazole biosynthesis protein ThiG, at 933,577 to 934,344
_c09070
Position (kb)
931
932
933 Strain fitness (log2 ratio)
-1
0
1
2 at 931.018 kb on - strand at 931.064 kb on + strand at 931.069 kb on + strand at 931.077 kb on - strand at 931.103 kb on + strand at 931.130 kb on - strand at 931.199 kb on + strand at 931.199 kb on + strand at 931.255 kb on + strand at 931.255 kb on + strand at 931.316 kb on - strand, within PGA1_c09030 at 931.336 kb on + strand, within PGA1_c09030 at 931.414 kb on - strand, within PGA1_c09030 at 931.418 kb on + strand, within PGA1_c09030 at 931.426 kb on - strand, within PGA1_c09030 at 931.426 kb on - strand, within PGA1_c09030 at 931.480 kb on + strand, within PGA1_c09030 at 931.490 kb on + strand, within PGA1_c09030 at 931.490 kb on + strand, within PGA1_c09030 at 931.613 kb on - strand, within PGA1_c09030 at 931.667 kb on - strand, within PGA1_c09030 at 931.848 kb on - strand, within PGA1_c09030 at 931.880 kb on + strand, within PGA1_c09030 at 931.922 kb on - strand at 931.942 kb on + strand at 931.947 kb on + strand at 931.947 kb on + strand at 931.947 kb on + strand at 931.955 kb on - strand at 931.996 kb on + strand at 932.004 kb on - strand at 932.004 kb on - strand at 932.009 kb on - strand at 932.016 kb on + strand at 932.068 kb on - strand at 932.091 kb on + strand at 932.091 kb on + strand at 932.091 kb on + strand at 932.099 kb on - strand, within PGA1_c09040 at 932.099 kb on - strand, within PGA1_c09040 at 932.099 kb on - strand, within PGA1_c09040 at 932.099 kb on - strand, within PGA1_c09040 at 932.099 kb on - strand, within PGA1_c09040 at 932.184 kb on - strand, within PGA1_c09040 at 932.207 kb on - strand, within PGA1_c09040 at 932.222 kb on - strand, within PGA1_c09040 at 932.267 kb on + strand, within PGA1_c09040 at 932.267 kb on + strand, within PGA1_c09040 at 932.275 kb on - strand, within PGA1_c09040 at 932.353 kb on - strand, within PGA1_c09040 at 932.436 kb on + strand, within PGA1_c09040 at 932.444 kb on - strand, within PGA1_c09040 at 932.496 kb on + strand, within PGA1_c09040 at 932.541 kb on - strand, within PGA1_c09040 at 932.576 kb on + strand, within PGA1_c09040 at 932.576 kb on + strand, within PGA1_c09040 at 932.578 kb on + strand, within PGA1_c09040 at 932.578 kb on + strand, within PGA1_c09040 at 932.578 kb on + strand, within PGA1_c09040 at 932.578 kb on + strand, within PGA1_c09040 at 932.580 kb on + strand, within PGA1_c09040 at 932.584 kb on - strand, within PGA1_c09040 at 932.586 kb on - strand, within PGA1_c09040 at 932.586 kb on - strand, within PGA1_c09040 at 932.586 kb on - strand, within PGA1_c09040 at 932.588 kb on + strand, within PGA1_c09040 at 932.588 kb on + strand, within PGA1_c09040 at 932.596 kb on - strand, within PGA1_c09040 at 932.708 kb on + strand, within PGA1_c09040 at 932.722 kb on + strand, within PGA1_c09040 at 932.757 kb on + strand, within PGA1_c09040 at 932.760 kb on - strand, within PGA1_c09040 at 932.786 kb on - strand, within PGA1_c09040 at 932.789 kb on + strand, within PGA1_c09040 at 932.800 kb on + strand, within PGA1_c09040 at 932.849 kb on + strand, within PGA1_c09040 at 932.859 kb on + strand, within PGA1_c09040 at 932.859 kb on + strand, within PGA1_c09040 at 932.862 kb on - strand, within PGA1_c09040 at 932.981 kb on - strand at 932.981 kb on - strand at 932.981 kb on - strand at 933.114 kb on + strand, within PGA1_c09050 at 933.146 kb on + strand, within PGA1_c09050 at 933.154 kb on - strand, within PGA1_c09050 at 933.184 kb on - strand, within PGA1_c09050 at 933.186 kb on + strand, within PGA1_c09050 at 933.186 kb on + strand, within PGA1_c09050 at 933.213 kb on - strand, within PGA1_c09050 at 933.275 kb on + strand, within PGA1_c09050 at 933.283 kb on - strand, within PGA1_c09050 at 933.283 kb on - strand, within PGA1_c09050 at 933.312 kb on - strand, within PGA1_c09050 at 933.314 kb on + strand, within PGA1_c09050 at 933.314 kb on + strand, within PGA1_c09050 at 933.322 kb on - strand, within PGA1_c09050 at 933.322 kb on - strand, within PGA1_c09050 at 933.484 kb on + strand, within PGA1_c09050 at 933.488 kb on + strand, within PGA1_c09050 at 933.518 kb on + strand at 933.575 kb on + strand at 933.580 kb on + strand at 933.581 kb on - strand at 933.583 kb on - strand at 933.583 kb on - strand at 933.583 kb on - strand at 933.583 kb on - strand at 933.603 kb on - strand at 933.689 kb on + strand, within PGA1_c09070 at 933.702 kb on - strand, within PGA1_c09070 at 933.731 kb on + strand, within PGA1_c09070 at 933.731 kb on + strand, within PGA1_c09070 at 933.739 kb on - strand, within PGA1_c09070 at 933.767 kb on + strand, within PGA1_c09070 at 933.862 kb on + strand, within PGA1_c09070 at 933.865 kb on - strand, within PGA1_c09070 at 933.878 kb on + strand, within PGA1_c09070 at 933.881 kb on - strand, within PGA1_c09070 at 933.901 kb on - strand, within PGA1_c09070 at 933.948 kb on + strand, within PGA1_c09070 at 933.972 kb on + strand, within PGA1_c09070 at 933.978 kb on - strand, within PGA1_c09070 at 933.980 kb on - strand, within PGA1_c09070
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Gentamicin 0.004 mg/ml remove 931,018 - -0.2 931,064 + -0.6 931,069 + -0.1 931,077 - -0.8 931,103 + +0.6 931,130 - +0.6 931,199 + -0.7 931,199 + +0.1 931,255 + +0.8 931,255 + -0.2 931,316 - PGA1_c09030 0.14 +1.6 931,336 + PGA1_c09030 0.17 -0.2 931,414 - PGA1_c09030 0.27 +1.4 931,418 + PGA1_c09030 0.27 -0.2 931,426 - PGA1_c09030 0.28 -0.5 931,426 - PGA1_c09030 0.28 +1.1 931,480 + PGA1_c09030 0.35 +0.8 931,490 + PGA1_c09030 0.36 +0.9 931,490 + PGA1_c09030 0.36 -0.0 931,613 - PGA1_c09030 0.52 +0.9 931,667 - PGA1_c09030 0.58 +1.1 931,848 - PGA1_c09030 0.81 +1.8 931,880 + PGA1_c09030 0.85 -0.4 931,922 - +1.1 931,942 + -1.4 931,947 + +1.0 931,947 + +0.8 931,947 + +0.6 931,955 - +0.7 931,996 + +1.0 932,004 - +0.6 932,004 - -0.1 932,009 - +1.2 932,016 + +1.1 932,068 - +0.6 932,091 + +0.5 932,091 + +0.6 932,091 + +0.1 932,099 - PGA1_c09040 0.11 +0.6 932,099 - PGA1_c09040 0.11 +1.2 932,099 - PGA1_c09040 0.11 +1.3 932,099 - PGA1_c09040 0.11 +1.1 932,099 - PGA1_c09040 0.11 +1.4 932,184 - PGA1_c09040 0.19 +0.5 932,207 - PGA1_c09040 0.21 +1.0 932,222 - PGA1_c09040 0.23 -0.1 932,267 + PGA1_c09040 0.27 +1.3 932,267 + PGA1_c09040 0.27 -0.4 932,275 - PGA1_c09040 0.28 +1.5 932,353 - PGA1_c09040 0.36 +1.5 932,436 + PGA1_c09040 0.45 +1.4 932,444 - PGA1_c09040 0.45 +0.9 932,496 + PGA1_c09040 0.51 +1.3 932,541 - PGA1_c09040 0.55 +0.2 932,576 + PGA1_c09040 0.59 +1.2 932,576 + PGA1_c09040 0.59 +1.0 932,578 + PGA1_c09040 0.59 +0.2 932,578 + PGA1_c09040 0.59 +0.5 932,578 + PGA1_c09040 0.59 +0.8 932,578 + PGA1_c09040 0.59 +1.3 932,580 + PGA1_c09040 0.59 +0.6 932,584 - PGA1_c09040 0.59 +0.5 932,586 - PGA1_c09040 0.60 +0.8 932,586 - PGA1_c09040 0.60 +1.4 932,586 - PGA1_c09040 0.60 +0.6 932,588 + PGA1_c09040 0.60 +1.0 932,588 + PGA1_c09040 0.60 +0.9 932,596 - PGA1_c09040 0.61 +1.4 932,708 + PGA1_c09040 0.72 +1.5 932,722 + PGA1_c09040 0.73 +0.3 932,757 + PGA1_c09040 0.77 +0.1 932,760 - PGA1_c09040 0.77 +1.2 932,786 - PGA1_c09040 0.80 -0.2 932,789 + PGA1_c09040 0.80 +0.0 932,800 + PGA1_c09040 0.81 +0.9 932,849 + PGA1_c09040 0.86 +1.6 932,859 + PGA1_c09040 0.87 +0.7 932,859 + PGA1_c09040 0.87 +0.9 932,862 - PGA1_c09040 0.88 +0.8 932,981 - +1.1 932,981 - +0.6 932,981 - +1.4 933,114 + PGA1_c09050 0.26 +1.0 933,146 + PGA1_c09050 0.31 +0.2 933,154 - PGA1_c09050 0.33 +1.0 933,184 - PGA1_c09050 0.38 -0.2 933,186 + PGA1_c09050 0.38 +0.3 933,186 + PGA1_c09050 0.38 +0.8 933,213 - PGA1_c09050 0.43 +1.1 933,275 + PGA1_c09050 0.53 +1.9 933,283 - PGA1_c09050 0.54 +0.7 933,283 - PGA1_c09050 0.54 +1.0 933,312 - PGA1_c09050 0.59 +1.2 933,314 + PGA1_c09050 0.60 +0.3 933,314 + PGA1_c09050 0.60 +1.2 933,322 - PGA1_c09050 0.61 +2.4 933,322 - PGA1_c09050 0.61 +0.8 933,484 + PGA1_c09050 0.88 +1.1 933,488 + PGA1_c09050 0.89 +0.3 933,518 + +0.6 933,575 + -0.1 933,580 + -0.0 933,581 - -1.2 933,583 - -0.3 933,583 - +0.7 933,583 - -0.1 933,583 - +0.3 933,603 - +1.2 933,689 + PGA1_c09070 0.15 +0.9 933,702 - PGA1_c09070 0.16 +0.7 933,731 + PGA1_c09070 0.20 +0.2 933,731 + PGA1_c09070 0.20 +0.1 933,739 - PGA1_c09070 0.21 +1.9 933,767 + PGA1_c09070 0.25 +0.4 933,862 + PGA1_c09070 0.37 +0.1 933,865 - PGA1_c09070 0.38 +1.1 933,878 + PGA1_c09070 0.39 +0.4 933,881 - PGA1_c09070 0.40 -0.4 933,901 - PGA1_c09070 0.42 +1.0 933,948 + PGA1_c09070 0.48 +0.5 933,972 + PGA1_c09070 0.51 +0.3 933,978 - PGA1_c09070 0.52 +1.1 933,980 - PGA1_c09070 0.52 +2.4
Or see this region's nucleotide sequence