Experiment: m.b. Gentamicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c04920 and PGA1_c04930 are separated by 135 nucleotides PGA1_c04930 and PGA1_c04940 are separated by 112 nucleotides PGA1_c04940 and PGA1_c04950 are separated by 296 nucleotides
PGA1_c04920: PGA1_c04920 - l-lactate dehydrogenase IldD, at 483,267 to 484,433
_c04920
PGA1_c04930: PGA1_c04930 - tryptophan synthase alpha chain, at 484,569 to 485,360
_c04930
PGA1_c04940: PGA1_c04940 - Lipid A 3-O-deacylase (PagL)., at 485,473 to 485,985
_c04940
PGA1_c04950: PGA1_c04950 - GTP-dependent nucleic acid-binding protein EngD, at 486,282 to 487,379
_c04950
Position (kb) 484
485
486 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 483.577 kb on - strand, within PGA1_c04920 at 483.584 kb on - strand, within PGA1_c04920 at 483.617 kb on + strand, within PGA1_c04920 at 483.631 kb on + strand, within PGA1_c04920 at 483.682 kb on + strand, within PGA1_c04920 at 483.742 kb on + strand, within PGA1_c04920 at 483.743 kb on + strand, within PGA1_c04920 at 483.751 kb on - strand, within PGA1_c04920 at 483.784 kb on + strand, within PGA1_c04920 at 483.851 kb on + strand, within PGA1_c04920 at 483.885 kb on + strand, within PGA1_c04920 at 483.893 kb on - strand, within PGA1_c04920 at 483.924 kb on + strand, within PGA1_c04920 at 483.924 kb on + strand, within PGA1_c04920 at 483.952 kb on - strand, within PGA1_c04920 at 484.003 kb on + strand, within PGA1_c04920 at 484.007 kb on + strand, within PGA1_c04920 at 484.050 kb on - strand, within PGA1_c04920 at 484.128 kb on - strand, within PGA1_c04920 at 484.138 kb on - strand, within PGA1_c04920 at 484.156 kb on + strand, within PGA1_c04920 at 484.202 kb on + strand, within PGA1_c04920 at 484.235 kb on - strand, within PGA1_c04920 at 484.249 kb on + strand, within PGA1_c04920 at 484.257 kb on + strand, within PGA1_c04920 at 484.257 kb on - strand, within PGA1_c04920 at 484.300 kb on - strand, within PGA1_c04920 at 484.329 kb on + strand at 484.435 kb on + strand at 484.443 kb on - strand at 484.443 kb on - strand at 484.443 kb on - strand at 484.453 kb on - strand at 484.499 kb on + strand at 484.507 kb on - strand at 484.526 kb on - strand at 485.363 kb on - strand at 485.392 kb on - strand at 485.412 kb on + strand at 485.420 kb on - strand at 485.420 kb on - strand at 485.426 kb on + strand at 485.453 kb on + strand at 485.517 kb on + strand at 485.522 kb on + strand at 485.530 kb on - strand, within PGA1_c04940 at 485.548 kb on + strand, within PGA1_c04940 at 485.557 kb on + strand, within PGA1_c04940 at 485.557 kb on + strand, within PGA1_c04940 at 485.557 kb on + strand, within PGA1_c04940 at 485.557 kb on + strand, within PGA1_c04940 at 485.557 kb on + strand, within PGA1_c04940 at 485.562 kb on + strand, within PGA1_c04940 at 485.562 kb on + strand, within PGA1_c04940 at 485.565 kb on - strand, within PGA1_c04940 at 485.565 kb on - strand, within PGA1_c04940 at 485.593 kb on + strand, within PGA1_c04940 at 485.604 kb on + strand, within PGA1_c04940 at 485.649 kb on + strand, within PGA1_c04940 at 485.676 kb on + strand, within PGA1_c04940 at 485.684 kb on - strand, within PGA1_c04940 at 485.740 kb on + strand, within PGA1_c04940 at 485.746 kb on - strand, within PGA1_c04940 at 485.766 kb on - strand, within PGA1_c04940 at 485.766 kb on - strand, within PGA1_c04940 at 485.853 kb on + strand, within PGA1_c04940 at 485.853 kb on + strand, within PGA1_c04940 at 485.904 kb on - strand, within PGA1_c04940 at 485.943 kb on - strand at 485.947 kb on + strand at 485.947 kb on + strand at 485.947 kb on + strand at 485.947 kb on + strand at 485.947 kb on + strand at 485.955 kb on - strand at 485.955 kb on - strand at 485.955 kb on - strand at 485.964 kb on + strand at 485.992 kb on + strand at 485.992 kb on + strand at 485.999 kb on - strand at 486.000 kb on - strand at 486.073 kb on - strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.140 kb on - strand at 486.173 kb on + strand at 486.190 kb on + strand at 486.202 kb on + strand at 486.212 kb on - strand at 486.216 kb on - strand at 486.234 kb on + strand at 486.244 kb on + strand at 486.255 kb on + strand at 486.273 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Gentamicin 0.004 mg/ml remove 483,577 - PGA1_c04920 0.27 +0.4 483,584 - PGA1_c04920 0.27 +0.8 483,617 + PGA1_c04920 0.30 +0.2 483,631 + PGA1_c04920 0.31 -0.4 483,682 + PGA1_c04920 0.36 -1.4 483,742 + PGA1_c04920 0.41 -1.2 483,743 + PGA1_c04920 0.41 +0.8 483,751 - PGA1_c04920 0.41 -2.4 483,784 + PGA1_c04920 0.44 -1.4 483,851 + PGA1_c04920 0.50 +0.4 483,885 + PGA1_c04920 0.53 -0.2 483,893 - PGA1_c04920 0.54 -0.7 483,924 + PGA1_c04920 0.56 +0.5 483,924 + PGA1_c04920 0.56 -1.2 483,952 - PGA1_c04920 0.59 +0.1 484,003 + PGA1_c04920 0.63 -0.5 484,007 + PGA1_c04920 0.63 +0.8 484,050 - PGA1_c04920 0.67 +0.1 484,128 - PGA1_c04920 0.74 +0.1 484,138 - PGA1_c04920 0.75 +0.4 484,156 + PGA1_c04920 0.76 -0.4 484,202 + PGA1_c04920 0.80 +0.7 484,235 - PGA1_c04920 0.83 -0.0 484,249 + PGA1_c04920 0.84 +0.5 484,257 + PGA1_c04920 0.85 -0.1 484,257 - PGA1_c04920 0.85 +0.0 484,300 - PGA1_c04920 0.89 -1.9 484,329 + +0.6 484,435 + -0.6 484,443 - -0.5 484,443 - -0.8 484,443 - -1.7 484,453 - +0.1 484,499 + +0.5 484,507 - +0.7 484,526 - -0.3 485,363 - +0.1 485,392 - +3.1 485,412 + +0.4 485,420 - -0.3 485,420 - -0.5 485,426 + +0.4 485,453 + +0.8 485,517 + -1.0 485,522 + -0.1 485,530 - PGA1_c04940 0.11 +1.1 485,548 + PGA1_c04940 0.15 +1.1 485,557 + PGA1_c04940 0.16 +0.1 485,557 + PGA1_c04940 0.16 +0.4 485,557 + PGA1_c04940 0.16 +1.2 485,557 + PGA1_c04940 0.16 +0.5 485,557 + PGA1_c04940 0.16 +0.3 485,562 + PGA1_c04940 0.17 -2.4 485,562 + PGA1_c04940 0.17 +1.1 485,565 - PGA1_c04940 0.18 -2.5 485,565 - PGA1_c04940 0.18 -0.6 485,593 + PGA1_c04940 0.23 -1.9 485,604 + PGA1_c04940 0.26 +1.2 485,649 + PGA1_c04940 0.34 +0.9 485,676 + PGA1_c04940 0.40 -0.4 485,684 - PGA1_c04940 0.41 -0.5 485,740 + PGA1_c04940 0.52 +0.3 485,746 - PGA1_c04940 0.53 +0.6 485,766 - PGA1_c04940 0.57 +0.8 485,766 - PGA1_c04940 0.57 +1.0 485,853 + PGA1_c04940 0.74 +1.0 485,853 + PGA1_c04940 0.74 -0.2 485,904 - PGA1_c04940 0.84 +1.0 485,943 - +0.3 485,947 + +1.5 485,947 + +0.7 485,947 + -0.1 485,947 + +0.7 485,947 + +0.6 485,955 - +1.5 485,955 - -0.2 485,955 - +1.4 485,964 + -0.5 485,992 + -0.8 485,992 + +0.4 485,999 - +0.5 486,000 - +0.0 486,073 - +0.6 486,076 + +0.3 486,076 + -0.0 486,076 + -1.6 486,076 + +1.0 486,076 + +0.9 486,076 + -0.3 486,076 + +1.4 486,076 + +0.9 486,076 + +0.4 486,076 + +0.4 486,076 + -0.4 486,076 + +0.7 486,076 + +0.4 486,076 + -0.2 486,076 + +1.3 486,076 + -0.9 486,076 + +0.5 486,076 + +1.0 486,076 + +0.4 486,076 + +0.2 486,076 + -0.1 486,076 + +1.0 486,076 + +0.3 486,076 + +0.7 486,076 + +0.8 486,076 + -0.2 486,076 + +0.7 486,076 + +0.3 486,076 + -2.4 486,084 - +0.1 486,084 - -0.1 486,084 - -0.5 486,084 - +1.5 486,084 - +0.5 486,084 - -0.8 486,084 - +0.5 486,084 - +0.0 486,084 - +0.5 486,084 - +0.5 486,084 - +0.5 486,084 - +1.0 486,084 - -1.0 486,084 - +0.4 486,084 - -0.2 486,084 - +0.2 486,084 - -0.2 486,084 - +0.9 486,084 - +0.8 486,084 - +0.4 486,084 - +0.1 486,084 - +0.3 486,084 - +0.3 486,084 - +1.2 486,084 - -0.1 486,084 - -0.0 486,084 - +0.6 486,084 - +0.6 486,084 - +1.1 486,084 - +0.5 486,084 - -0.7 486,084 - -0.8 486,084 - -0.6 486,084 - -0.2 486,084 - +0.2 486,084 - +0.2 486,084 - +1.1 486,140 - -2.4 486,173 + -1.4 486,190 + +0.2 486,202 + +0.0 486,212 - -0.3 486,216 - +0.1 486,234 + -0.5 486,244 + +0.6 486,255 + +0.2 486,273 - -0.6
Or see this region's nucleotide sequence