Experiment: m.b. Gentamicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c20350 and PGA1_c20360 are separated by 308 nucleotides PGA1_c20360 and PGA1_c20370 are separated by 135 nucleotides PGA1_c20370 and PGA1_c20380 are separated by 87 nucleotides PGA1_c20380 and PGA1_c20390 are separated by 50 nucleotides PGA1_c20390 and PGA1_c20400 are separated by 331 nucleotides
PGA1_c20350: PGA1_c20350 - 5-aminolevulinate synthase HemA, at 2,115,133 to 2,116,362
_c20350
PGA1_c20360: PGA1_c20360 - putative glutamate carboxypeptidase, at 2,116,671 to 2,118,062
_c20360
PGA1_c20370: PGA1_c20370 - tRNA-Asp, at 2,118,198 to 2,118,274
_c20370
PGA1_c20380: PGA1_c20380 - tRNA-Asp, at 2,118,362 to 2,118,438
_c20380
PGA1_c20390: PGA1_c20390 - tRNA-Asp, at 2,118,489 to 2,118,565
_c20390
PGA1_c20400: PGA1_c20400 - hypothetical protein, at 2,118,897 to 2,119,268
_c20400
Position (kb)
2116
2117
2118
2119 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2116.478 kb on - strand at 2116.485 kb on - strand at 2116.497 kb on - strand at 2116.598 kb on + strand at 2116.606 kb on - strand at 2116.621 kb on + strand at 2116.623 kb on + strand at 2116.644 kb on + strand at 2116.658 kb on - strand at 2116.694 kb on - strand at 2116.736 kb on - strand at 2116.784 kb on - strand at 2116.858 kb on + strand, within PGA1_c20360 at 2116.865 kb on - strand, within PGA1_c20360 at 2116.900 kb on + strand, within PGA1_c20360 at 2116.900 kb on + strand, within PGA1_c20360 at 2116.908 kb on - strand, within PGA1_c20360 at 2116.922 kb on + strand, within PGA1_c20360 at 2116.922 kb on + strand, within PGA1_c20360 at 2116.930 kb on - strand, within PGA1_c20360 at 2116.957 kb on + strand, within PGA1_c20360 at 2117.164 kb on + strand, within PGA1_c20360 at 2117.184 kb on - strand, within PGA1_c20360 at 2117.199 kb on - strand, within PGA1_c20360 at 2117.214 kb on + strand, within PGA1_c20360 at 2117.214 kb on + strand, within PGA1_c20360 at 2117.222 kb on - strand, within PGA1_c20360 at 2117.222 kb on - strand, within PGA1_c20360 at 2117.344 kb on + strand, within PGA1_c20360 at 2117.352 kb on - strand, within PGA1_c20360 at 2117.372 kb on + strand, within PGA1_c20360 at 2117.509 kb on - strand, within PGA1_c20360 at 2117.520 kb on + strand, within PGA1_c20360 at 2117.520 kb on + strand, within PGA1_c20360 at 2117.528 kb on - strand, within PGA1_c20360 at 2117.567 kb on + strand, within PGA1_c20360 at 2117.732 kb on - strand, within PGA1_c20360 at 2117.746 kb on - strand, within PGA1_c20360 at 2117.799 kb on - strand, within PGA1_c20360 at 2117.870 kb on + strand, within PGA1_c20360 at 2117.878 kb on + strand, within PGA1_c20360 at 2118.084 kb on + strand at 2118.086 kb on + strand at 2118.088 kb on + strand at 2118.091 kb on + strand at 2118.091 kb on + strand at 2118.091 kb on + strand at 2118.091 kb on + strand at 2118.091 kb on + strand at 2118.099 kb on - strand at 2118.124 kb on + strand at 2118.124 kb on + strand at 2118.167 kb on - strand at 2118.222 kb on - strand, within PGA1_c20370 at 2118.249 kb on + strand, within PGA1_c20370 at 2118.258 kb on + strand, within PGA1_c20370 at 2118.263 kb on + strand, within PGA1_c20370 at 2118.263 kb on + strand, within PGA1_c20370 at 2118.282 kb on + strand at 2118.292 kb on + strand at 2118.750 kb on - strand at 2118.776 kb on + strand at 2118.784 kb on - strand at 2118.784 kb on - strand at 2118.785 kb on - strand at 2118.815 kb on + strand at 2118.818 kb on - strand at 2118.848 kb on - strand at 2118.867 kb on + strand at 2118.875 kb on - strand at 2118.875 kb on - strand at 2118.875 kb on - strand at 2118.875 kb on - strand at 2118.875 kb on - strand at 2118.875 kb on - strand at 2118.898 kb on - strand at 2118.919 kb on - strand at 2118.919 kb on - strand at 2118.919 kb on - strand at 2118.925 kb on - strand at 2118.928 kb on + strand at 2119.024 kb on + strand, within PGA1_c20400 at 2119.042 kb on - strand, within PGA1_c20400 at 2119.057 kb on + strand, within PGA1_c20400
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Gentamicin 0.004 mg/ml remove 2,116,478 - -0.6 2,116,485 - +0.2 2,116,497 - -0.1 2,116,598 + +0.2 2,116,606 - +0.9 2,116,621 + -0.7 2,116,623 + +0.9 2,116,644 + -0.7 2,116,658 - -1.9 2,116,694 - -0.6 2,116,736 - +0.2 2,116,784 - -0.4 2,116,858 + PGA1_c20360 0.13 +0.3 2,116,865 - PGA1_c20360 0.14 -0.5 2,116,900 + PGA1_c20360 0.16 -0.1 2,116,900 + PGA1_c20360 0.16 -0.7 2,116,908 - PGA1_c20360 0.17 +0.5 2,116,922 + PGA1_c20360 0.18 -0.2 2,116,922 + PGA1_c20360 0.18 -1.2 2,116,930 - PGA1_c20360 0.19 -0.9 2,116,957 + PGA1_c20360 0.21 -0.7 2,117,164 + PGA1_c20360 0.35 -0.8 2,117,184 - PGA1_c20360 0.37 -0.3 2,117,199 - PGA1_c20360 0.38 -0.1 2,117,214 + PGA1_c20360 0.39 +0.1 2,117,214 + PGA1_c20360 0.39 -0.5 2,117,222 - PGA1_c20360 0.40 +1.1 2,117,222 - PGA1_c20360 0.40 +0.4 2,117,344 + PGA1_c20360 0.48 -1.4 2,117,352 - PGA1_c20360 0.49 -0.6 2,117,372 + PGA1_c20360 0.50 +0.1 2,117,509 - PGA1_c20360 0.60 +0.4 2,117,520 + PGA1_c20360 0.61 -0.2 2,117,520 + PGA1_c20360 0.61 +0.5 2,117,528 - PGA1_c20360 0.62 -1.1 2,117,567 + PGA1_c20360 0.64 -0.6 2,117,732 - PGA1_c20360 0.76 +0.2 2,117,746 - PGA1_c20360 0.77 -0.6 2,117,799 - PGA1_c20360 0.81 -0.5 2,117,870 + PGA1_c20360 0.86 -0.7 2,117,878 + PGA1_c20360 0.87 +0.6 2,118,084 + +0.4 2,118,086 + +0.5 2,118,088 + -0.2 2,118,091 + +0.1 2,118,091 + -0.2 2,118,091 + +0.8 2,118,091 + +0.0 2,118,091 + -0.4 2,118,099 - +1.0 2,118,124 + +2.2 2,118,124 + +0.4 2,118,167 - +0.2 2,118,222 - PGA1_c20370 0.31 -0.2 2,118,249 + PGA1_c20370 0.66 +0.8 2,118,258 + PGA1_c20370 0.78 +0.6 2,118,263 + PGA1_c20370 0.84 -0.9 2,118,263 + PGA1_c20370 0.84 -0.4 2,118,282 + +0.1 2,118,292 + +0.1 2,118,750 - -0.8 2,118,776 + +0.8 2,118,784 - +0.2 2,118,784 - +0.2 2,118,785 - +2.4 2,118,815 + -0.4 2,118,818 - -0.8 2,118,848 - -0.2 2,118,867 + +0.8 2,118,875 - -1.3 2,118,875 - +1.4 2,118,875 - -0.3 2,118,875 - +0.3 2,118,875 - -0.6 2,118,875 - +0.1 2,118,898 - -0.5 2,118,919 - +0.6 2,118,919 - -0.9 2,118,919 - +0.8 2,118,925 - +0.4 2,118,928 + +0.4 2,119,024 + PGA1_c20400 0.34 -0.4 2,119,042 - PGA1_c20400 0.39 -0.4 2,119,057 + PGA1_c20400 0.43 -1.6
Or see this region's nucleotide sequence