Experiment: LB, pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21550 and BWI76_RS21555 are separated by 13 nucleotides BWI76_RS21555 and BWI76_RS21560 are separated by 86 nucleotides BWI76_RS21560 and BWI76_RS21565 are separated by 147 nucleotides BWI76_RS21565 and BWI76_RS21570 are separated by 65 nucleotides BWI76_RS21570 and BWI76_RS21575 overlap by 11 nucleotides
BWI76_RS21550: BWI76_RS21550 - inorganic polyphosphate kinase, at 4,511,927 to 4,512,073
_RS21550
BWI76_RS21555: BWI76_RS21555 - NAD(+) kinase, at 4,512,087 to 4,512,965
_RS21555
BWI76_RS21560: BWI76_RS21560 - DNA repair protein RecN, at 4,513,052 to 4,514,713
_RS21560
BWI76_RS21565: BWI76_RS21565 - outer membrane protein assembly factor BamE, at 4,514,861 to 4,515,202
_RS21565
BWI76_RS21570: BWI76_RS21570 - RnfH family protein, at 4,515,268 to 4,515,558
_RS21570
BWI76_RS21575: BWI76_RS21575 - hypothetical protein, at 4,515,548 to 4,516,024
_RS21575
Position (kb) 4513
4514
4515 Strain fitness (log2 ratio) -1
0
1
2 at 4513.141 kb on + strand at 4513.141 kb on + strand at 4513.823 kb on + strand, within BWI76_RS21560 at 4513.838 kb on - strand, within BWI76_RS21560 at 4514.133 kb on - strand, within BWI76_RS21560 at 4514.506 kb on + strand, within BWI76_RS21560 at 4514.549 kb on - strand at 4514.600 kb on + strand at 4514.822 kb on + strand at 4515.166 kb on - strand, within BWI76_RS21565 at 4515.280 kb on + strand at 4515.361 kb on - strand at 4515.402 kb on + strand, within BWI76_RS21570 at 4515.489 kb on + strand, within BWI76_RS21570 at 4515.558 kb on + strand at 4515.566 kb on - strand at 4515.566 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH8 remove 4,513,141 + -0.4 4,513,141 + -0.2 4,513,823 + BWI76_RS21560 0.46 -0.6 4,513,838 - BWI76_RS21560 0.47 -0.1 4,514,133 - BWI76_RS21560 0.65 +0.0 4,514,506 + BWI76_RS21560 0.87 -1.3 4,514,549 - +0.3 4,514,600 + -0.2 4,514,822 + -0.1 4,515,166 - BWI76_RS21565 0.89 -0.1 4,515,280 + +0.2 4,515,361 - +2.0 4,515,402 + BWI76_RS21570 0.46 -0.5 4,515,489 + BWI76_RS21570 0.76 -0.4 4,515,558 + -0.2 4,515,566 - -1.0 4,515,566 - +0.2
Or see this region's nucleotide sequence