Experiment: LB, pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09020 and BWI76_RS09025 are separated by 70 nucleotides BWI76_RS09025 and BWI76_RS09030 are separated by 43 nucleotides BWI76_RS09030 and BWI76_RS09035 are separated by 190 nucleotides
BWI76_RS09020: BWI76_RS09020 - 6-phospho-beta-glucosidase, at 1,900,097 to 1,901,473
_RS09020
BWI76_RS09025: BWI76_RS09025 - GntR family transcriptional regulator, at 1,901,544 to 1,902,260
_RS09025
BWI76_RS09030: BWI76_RS09030 - transpeptidase, at 1,902,304 to 1,903,218
_RS09030
BWI76_RS09035: BWI76_RS09035 - protein tyrosine/serine phosphatase, at 1,903,409 to 1,904,191
_RS09035
Position (kb) 1902
1903
1904 Strain fitness (log2 ratio) -1
0
1 at 1901.409 kb on - strand at 1901.496 kb on - strand at 1901.501 kb on - strand at 1901.700 kb on + strand, within BWI76_RS09025 at 1901.735 kb on - strand, within BWI76_RS09025 at 1902.048 kb on - strand, within BWI76_RS09025 at 1902.391 kb on - strand at 1902.523 kb on + strand, within BWI76_RS09030 at 1902.598 kb on + strand, within BWI76_RS09030 at 1902.640 kb on + strand, within BWI76_RS09030 at 1902.840 kb on + strand, within BWI76_RS09030 at 1902.848 kb on - strand, within BWI76_RS09030 at 1902.913 kb on + strand, within BWI76_RS09030 at 1903.201 kb on + strand at 1903.632 kb on + strand, within BWI76_RS09035 at 1903.632 kb on + strand, within BWI76_RS09035 at 1903.717 kb on - strand, within BWI76_RS09035 at 1903.737 kb on + strand, within BWI76_RS09035 at 1903.883 kb on - strand, within BWI76_RS09035 at 1904.009 kb on + strand, within BWI76_RS09035
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH8 remove 1,901,409 - +0.0 1,901,496 - -0.0 1,901,501 - +0.2 1,901,700 + BWI76_RS09025 0.22 +0.2 1,901,735 - BWI76_RS09025 0.27 +0.3 1,902,048 - BWI76_RS09025 0.70 -0.5 1,902,391 - +0.1 1,902,523 + BWI76_RS09030 0.24 -0.8 1,902,598 + BWI76_RS09030 0.32 -0.6 1,902,640 + BWI76_RS09030 0.37 -0.2 1,902,840 + BWI76_RS09030 0.59 -1.6 1,902,848 - BWI76_RS09030 0.59 -0.6 1,902,913 + BWI76_RS09030 0.67 -0.9 1,903,201 + -0.7 1,903,632 + BWI76_RS09035 0.28 -0.2 1,903,632 + BWI76_RS09035 0.28 +0.8 1,903,717 - BWI76_RS09035 0.39 -0.5 1,903,737 + BWI76_RS09035 0.42 -1.5 1,903,883 - BWI76_RS09035 0.61 +0.6 1,904,009 + BWI76_RS09035 0.77 +1.0
Or see this region's nucleotide sequence