Experiment: MoLS4 with L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVUA0141 and nifA-2 are separated by 597 nucleotides nifA-2 and DVUA0144 are separated by 214 nucleotides DVUA0144 and DORF2315 are separated by 499 nucleotides
DVUA0141: DVUA0141 - hypothetical protein (TIGR), at 183,952 to 184,188
DVUA0141
DVUA0143: nifA-2 - nif-specific regulatory protein (TIGR), at 184,786 to 186,333
nifA-2
DVUA0144: DVUA0144 - hypothetical protein (TIGR), at 186,548 to 186,691
DVUA0144
DORF2315: DORF2315 - no description, at 187,191 to 187,322
DORF2315
Position (kb) 184
185
186
187 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 184.031 kb on - strand, within DVUA0141 at 184.133 kb on + strand, within DVUA0141 at 184.156 kb on - strand, within DVUA0141 at 184.317 kb on + strand at 184.325 kb on - strand at 184.348 kb on + strand at 184.348 kb on + strand at 184.391 kb on - strand at 184.497 kb on - strand at 184.583 kb on + strand at 184.661 kb on + strand at 184.669 kb on - strand at 184.669 kb on - strand at 184.699 kb on + strand at 184.774 kb on + strand at 184.791 kb on - strand at 184.823 kb on + strand at 184.883 kb on + strand at 184.888 kb on + strand at 184.891 kb on - strand at 184.891 kb on - strand at 184.891 kb on - strand at 184.940 kb on + strand at 184.941 kb on - strand, within nifA-2 at 184.943 kb on - strand, within nifA-2 at 184.970 kb on - strand, within nifA-2 at 185.156 kb on - strand, within nifA-2 at 185.177 kb on + strand, within nifA-2 at 185.192 kb on + strand, within nifA-2 at 185.202 kb on + strand, within nifA-2 at 185.453 kb on - strand, within nifA-2 at 185.457 kb on - strand, within nifA-2 at 185.472 kb on - strand, within nifA-2 at 185.474 kb on + strand, within nifA-2 at 185.482 kb on - strand, within nifA-2 at 185.493 kb on - strand, within nifA-2 at 185.502 kb on - strand, within nifA-2 at 185.512 kb on - strand, within nifA-2 at 185.587 kb on - strand, within nifA-2 at 185.638 kb on - strand, within nifA-2 at 185.665 kb on + strand, within nifA-2 at 185.673 kb on - strand, within nifA-2 at 185.700 kb on + strand, within nifA-2 at 185.837 kb on + strand, within nifA-2 at 185.906 kb on + strand, within nifA-2 at 186.033 kb on - strand, within nifA-2 at 186.053 kb on + strand, within nifA-2 at 186.053 kb on + strand, within nifA-2 at 186.053 kb on + strand, within nifA-2 at 186.053 kb on + strand, within nifA-2 at 186.053 kb on + strand, within nifA-2 at 186.053 kb on + strand, within nifA-2 at 186.061 kb on - strand, within nifA-2 at 186.068 kb on + strand, within nifA-2 at 186.068 kb on + strand, within nifA-2 at 186.314 kb on - strand at 186.370 kb on - strand at 186.466 kb on + strand at 186.497 kb on + strand at 186.527 kb on + strand at 186.547 kb on - strand at 186.629 kb on + strand, within DVUA0144 at 186.631 kb on + strand, within DVUA0144 at 186.699 kb on + strand at 186.726 kb on - strand at 186.735 kb on + strand at 186.759 kb on + strand at 186.773 kb on - strand at 186.787 kb on + strand at 186.805 kb on - strand at 186.845 kb on + strand at 186.850 kb on + strand at 186.858 kb on - strand at 186.920 kb on + strand at 186.975 kb on + strand at 186.977 kb on - strand at 187.014 kb on + strand at 187.099 kb on - strand at 187.109 kb on - strand at 187.288 kb on - strand, within DORF2315 at 187.294 kb on + strand, within DORF2315
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Alanine (N) remove 184,031 - DVUA0141 0.33 -0.5 184,133 + DVUA0141 0.76 -0.4 184,156 - DVUA0141 0.86 -1.4 184,317 + -0.2 184,325 - -0.6 184,348 + -0.2 184,348 + -0.5 184,391 - -0.6 184,497 - -0.5 184,583 + -0.3 184,661 + +0.0 184,669 - +0.2 184,669 - -0.6 184,699 + +2.2 184,774 + -0.6 184,791 - +0.6 184,823 + -4.2 184,883 + -0.6 184,888 + -0.7 184,891 - -3.8 184,891 - +1.2 184,891 - -4.7 184,940 + -4.5 184,941 - nifA-2 DVUA0143 0.10 +1.2 184,943 - nifA-2 DVUA0143 0.10 -2.5 184,970 - nifA-2 DVUA0143 0.12 -3.9 185,156 - nifA-2 DVUA0143 0.24 -2.7 185,177 + nifA-2 DVUA0143 0.25 -4.6 185,192 + nifA-2 DVUA0143 0.26 -1.3 185,202 + nifA-2 DVUA0143 0.27 -3.3 185,453 - nifA-2 DVUA0143 0.43 -1.8 185,457 - nifA-2 DVUA0143 0.43 -2.6 185,472 - nifA-2 DVUA0143 0.44 -1.4 185,474 + nifA-2 DVUA0143 0.44 -3.5 185,482 - nifA-2 DVUA0143 0.45 -3.6 185,493 - nifA-2 DVUA0143 0.46 -4.1 185,502 - nifA-2 DVUA0143 0.46 -4.1 185,512 - nifA-2 DVUA0143 0.47 -4.6 185,587 - nifA-2 DVUA0143 0.52 -4.0 185,638 - nifA-2 DVUA0143 0.55 -3.5 185,665 + nifA-2 DVUA0143 0.57 -4.4 185,673 - nifA-2 DVUA0143 0.57 -4.4 185,700 + nifA-2 DVUA0143 0.59 -4.4 185,837 + nifA-2 DVUA0143 0.68 -3.5 185,906 + nifA-2 DVUA0143 0.72 -2.7 186,033 - nifA-2 DVUA0143 0.81 -4.9 186,053 + nifA-2 DVUA0143 0.82 -3.7 186,053 + nifA-2 DVUA0143 0.82 -4.5 186,053 + nifA-2 DVUA0143 0.82 -3.0 186,053 + nifA-2 DVUA0143 0.82 -4.3 186,053 + nifA-2 DVUA0143 0.82 -4.7 186,053 + nifA-2 DVUA0143 0.82 -2.4 186,061 - nifA-2 DVUA0143 0.82 -0.9 186,068 + nifA-2 DVUA0143 0.83 -4.8 186,068 + nifA-2 DVUA0143 0.83 -4.6 186,314 - -3.4 186,370 - -1.9 186,466 + -1.6 186,497 + -3.4 186,527 + -2.0 186,547 - -1.5 186,629 + DVUA0144 0.56 -0.4 186,631 + DVUA0144 0.58 -0.6 186,699 + -0.9 186,726 - -0.5 186,735 + +0.8 186,759 + -0.3 186,773 - -0.5 186,787 + -0.5 186,805 - -1.4 186,845 + -0.8 186,850 + -2.0 186,858 - -0.4 186,920 + +1.3 186,975 + -0.2 186,977 - +1.6 187,014 + -0.3 187,099 - +0.3 187,109 - +0.7 187,288 - DORF2315 0.73 +1.8 187,294 + DORF2315 0.78 -0.5
Or see this region's nucleotide sequence