Experiment: MoLS4 with L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pabB and pabA are separated by 538 nucleotides pabA and DVU0365 overlap by 4 nucleotides DVU0365 and DVU0366 overlap by 10 nucleotides
DVU0363: pabB - para-aminobenzoate synthase, component I (TIGR), at 412,237 to 413,241
pabB
DVU0364: pabA - para-aminobenzoate/anthranilate synthase glutamine amidotransferase (TIGR), at 413,780 to 414,334
pabA
DVU0365: DVU0365 - conserved hypothetical protein (TIGR), at 414,331 to 415,086
DVU0365
DVU0366: DVU0366 - 5-formyltetrahydrofolate cyclo-ligase family protein (TIGR), at 415,077 to 415,928
DVU0366
Position (kb) 413
414
415 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 412.787 kb on + strand, within pabB at 412.828 kb on + strand, within pabB at 412.834 kb on - strand, within pabB at 412.901 kb on - strand, within pabB at 412.980 kb on + strand, within pabB at 412.983 kb on - strand, within pabB at 412.996 kb on - strand, within pabB at 412.996 kb on - strand, within pabB at 413.002 kb on + strand, within pabB at 413.019 kb on + strand, within pabB at 413.027 kb on - strand, within pabB at 413.094 kb on - strand, within pabB at 413.097 kb on + strand, within pabB at 413.128 kb on + strand, within pabB at 413.205 kb on + strand at 413.264 kb on + strand at 413.268 kb on - strand at 413.306 kb on - strand at 413.430 kb on - strand at 413.523 kb on - strand at 413.531 kb on - strand at 413.549 kb on - strand at 413.570 kb on + strand at 413.577 kb on + strand at 413.578 kb on - strand at 413.578 kb on - strand at 413.587 kb on - strand at 413.641 kb on + strand at 413.680 kb on + strand at 413.709 kb on - strand at 413.719 kb on - strand at 413.755 kb on + strand at 413.796 kb on - strand at 413.811 kb on - strand at 413.858 kb on - strand, within pabA at 413.935 kb on + strand, within pabA at 413.964 kb on + strand, within pabA at 413.977 kb on - strand, within pabA at 413.990 kb on - strand, within pabA at 414.007 kb on - strand, within pabA at 414.012 kb on + strand, within pabA at 414.018 kb on + strand, within pabA at 414.020 kb on - strand, within pabA at 414.103 kb on + strand, within pabA at 414.149 kb on - strand, within pabA at 414.186 kb on - strand, within pabA at 414.224 kb on + strand, within pabA at 414.275 kb on - strand, within pabA at 414.312 kb on + strand at 414.345 kb on + strand at 414.350 kb on + strand at 414.373 kb on + strand at 414.425 kb on - strand, within DVU0365 at 414.430 kb on - strand, within DVU0365 at 414.430 kb on - strand, within DVU0365 at 414.438 kb on - strand, within DVU0365 at 414.438 kb on - strand, within DVU0365 at 414.440 kb on - strand, within DVU0365 at 414.440 kb on - strand, within DVU0365 at 414.440 kb on - strand, within DVU0365 at 414.501 kb on + strand, within DVU0365 at 414.509 kb on - strand, within DVU0365 at 414.509 kb on - strand, within DVU0365 at 414.602 kb on - strand, within DVU0365 at 414.627 kb on + strand, within DVU0365 at 414.634 kb on + strand, within DVU0365 at 414.708 kb on + strand, within DVU0365 at 414.713 kb on + strand, within DVU0365 at 414.750 kb on - strand, within DVU0365 at 414.838 kb on + strand, within DVU0365 at 414.887 kb on - strand, within DVU0365 at 415.004 kb on - strand, within DVU0365 at 415.018 kb on - strand at 415.075 kb on + strand at 415.097 kb on + strand at 415.100 kb on - strand at 415.102 kb on + strand at 415.105 kb on - strand at 415.125 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Alanine (N) remove 412,787 + pabB DVU0363 0.55 -0.6 412,828 + pabB DVU0363 0.59 +0.4 412,834 - pabB DVU0363 0.59 -1.0 412,901 - pabB DVU0363 0.66 -0.2 412,980 + pabB DVU0363 0.74 -0.3 412,983 - pabB DVU0363 0.74 -1.0 412,996 - pabB DVU0363 0.76 -0.7 412,996 - pabB DVU0363 0.76 -0.2 413,002 + pabB DVU0363 0.76 -1.5 413,019 + pabB DVU0363 0.78 -0.5 413,027 - pabB DVU0363 0.79 -1.0 413,094 - pabB DVU0363 0.85 -0.4 413,097 + pabB DVU0363 0.86 -0.3 413,128 + pabB DVU0363 0.89 -0.8 413,205 + +0.2 413,264 + -0.8 413,268 - -0.5 413,306 - -1.5 413,430 - +0.1 413,523 - -1.2 413,531 - +0.2 413,549 - +0.3 413,570 + -0.4 413,577 + -0.6 413,578 - -0.2 413,578 - +0.6 413,587 - -1.4 413,641 + +0.4 413,680 + -0.3 413,709 - -0.1 413,719 - -1.2 413,755 + -0.4 413,796 - -0.3 413,811 - +0.3 413,858 - pabA DVU0364 0.14 +0.6 413,935 + pabA DVU0364 0.28 +1.4 413,964 + pabA DVU0364 0.33 -0.0 413,977 - pabA DVU0364 0.35 -0.4 413,990 - pabA DVU0364 0.38 -0.2 414,007 - pabA DVU0364 0.41 -0.4 414,012 + pabA DVU0364 0.42 -1.3 414,018 + pabA DVU0364 0.43 +0.8 414,020 - pabA DVU0364 0.43 -0.2 414,103 + pabA DVU0364 0.58 -3.2 414,149 - pabA DVU0364 0.66 -0.4 414,186 - pabA DVU0364 0.73 +0.3 414,224 + pabA DVU0364 0.80 -0.1 414,275 - pabA DVU0364 0.89 -0.5 414,312 + +1.2 414,345 + +0.1 414,350 + -1.0 414,373 + +0.1 414,425 - DVU0365 0.12 +1.4 414,430 - DVU0365 0.13 +0.7 414,430 - DVU0365 0.13 +0.8 414,438 - DVU0365 0.14 -0.7 414,438 - DVU0365 0.14 +0.1 414,440 - DVU0365 0.14 +0.0 414,440 - DVU0365 0.14 -0.9 414,440 - DVU0365 0.14 +0.2 414,501 + DVU0365 0.22 +1.5 414,509 - DVU0365 0.24 +0.1 414,509 - DVU0365 0.24 +0.8 414,602 - DVU0365 0.36 -0.0 414,627 + DVU0365 0.39 +0.4 414,634 + DVU0365 0.40 -0.6 414,708 + DVU0365 0.50 -0.2 414,713 + DVU0365 0.51 +0.2 414,750 - DVU0365 0.55 -0.3 414,838 + DVU0365 0.67 -0.3 414,887 - DVU0365 0.74 +0.1 415,004 - DVU0365 0.89 -0.3 415,018 - +0.0 415,075 + +0.1 415,097 + +0.1 415,100 - -0.9 415,102 + +1.7 415,105 - +2.5 415,125 + +1.3
Or see this region's nucleotide sequence