Experiment: MoLS4 with L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rbsA and DVU1071 are separated by 428 nucleotides DVU1071 and DVU1072 are separated by 61 nucleotides DVU1072 and DVU1073 are separated by 195 nucleotides
DVU1070: rbsA - branched chain amino acid ABC transporter, ATP-binding protein (TIGR), at 1,173,950 to 1,175,524
rbsA
DVU1071: DVU1071 - hypothetical protein (TIGR), at 1,175,953 to 1,176,543
DVU1071
DVU1072: DVU1072 - conserved hypothetical protein (TIGR), at 1,176,605 to 1,177,267
DVU1072
DVU1073: DVU1073 - conserved domain protein (TIGR), at 1,177,463 to 1,178,389
DVU1073
Position (kb)
1175
1176
1177 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1175.185 kb on - strand, within rbsA at 1175.300 kb on + strand, within rbsA at 1175.361 kb on + strand, within rbsA at 1175.361 kb on + strand, within rbsA at 1175.404 kb on - strand at 1175.423 kb on + strand at 1175.431 kb on - strand at 1175.490 kb on + strand at 1175.609 kb on - strand at 1175.631 kb on + strand at 1175.670 kb on + strand at 1175.675 kb on + strand at 1175.678 kb on - strand at 1175.872 kb on + strand at 1176.046 kb on - strand, within DVU1071 at 1176.056 kb on - strand, within DVU1071 at 1176.111 kb on + strand, within DVU1071 at 1176.229 kb on + strand, within DVU1071 at 1176.229 kb on + strand, within DVU1071 at 1176.237 kb on - strand, within DVU1071 at 1176.334 kb on + strand, within DVU1071 at 1176.383 kb on - strand, within DVU1071 at 1176.458 kb on - strand, within DVU1071 at 1176.571 kb on - strand at 1176.582 kb on + strand at 1176.614 kb on + strand at 1176.848 kb on - strand, within DVU1072 at 1176.848 kb on - strand, within DVU1072 at 1176.867 kb on + strand, within DVU1072 at 1176.875 kb on - strand, within DVU1072 at 1176.875 kb on - strand, within DVU1072 at 1176.882 kb on - strand, within DVU1072 at 1176.980 kb on - strand, within DVU1072 at 1177.011 kb on + strand, within DVU1072 at 1177.048 kb on - strand, within DVU1072 at 1177.088 kb on - strand, within DVU1072 at 1177.114 kb on - strand, within DVU1072 at 1177.138 kb on - strand, within DVU1072 at 1177.174 kb on + strand, within DVU1072 at 1177.218 kb on + strand at 1177.218 kb on + strand at 1177.351 kb on + strand at 1177.351 kb on + strand at 1177.351 kb on + strand at 1177.359 kb on - strand at 1177.442 kb on - strand at 1177.539 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Alanine (N) remove 1,175,185 - rbsA DVU1070 0.78 -0.1 1,175,300 + rbsA DVU1070 0.86 -1.0 1,175,361 + rbsA DVU1070 0.90 +0.8 1,175,361 + rbsA DVU1070 0.90 -2.2 1,175,404 - -1.8 1,175,423 + -1.5 1,175,431 - -0.9 1,175,490 + -1.1 1,175,609 - +0.9 1,175,631 + +0.8 1,175,670 + +0.1 1,175,675 + -0.1 1,175,678 - -1.1 1,175,872 + +2.1 1,176,046 - DVU1071 0.16 -0.1 1,176,056 - DVU1071 0.17 +1.6 1,176,111 + DVU1071 0.27 +0.4 1,176,229 + DVU1071 0.47 -0.1 1,176,229 + DVU1071 0.47 +0.1 1,176,237 - DVU1071 0.48 -0.5 1,176,334 + DVU1071 0.64 -0.2 1,176,383 - DVU1071 0.73 -0.0 1,176,458 - DVU1071 0.85 -0.4 1,176,571 - +0.5 1,176,582 + -0.1 1,176,614 + +0.1 1,176,848 - DVU1072 0.37 +1.4 1,176,848 - DVU1072 0.37 +0.3 1,176,867 + DVU1072 0.40 +0.8 1,176,875 - DVU1072 0.41 +0.7 1,176,875 - DVU1072 0.41 +0.8 1,176,882 - DVU1072 0.42 -1.1 1,176,980 - DVU1072 0.57 -0.3 1,177,011 + DVU1072 0.61 -0.6 1,177,048 - DVU1072 0.67 -0.6 1,177,088 - DVU1072 0.73 -1.5 1,177,114 - DVU1072 0.77 -0.5 1,177,138 - DVU1072 0.80 -0.6 1,177,174 + DVU1072 0.86 -0.4 1,177,218 + -0.1 1,177,218 + -0.3 1,177,351 + +0.8 1,177,351 + -0.5 1,177,351 + -0.4 1,177,359 - -0.3 1,177,442 - -0.9 1,177,539 - +0.2
Or see this region's nucleotide sequence