Experiment: MoLS4 with L-Glycine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0273 and DVU0274 are separated by 311 nucleotides DVU0274 and DVU0275 are separated by 294 nucleotides DVU0275 and tRNA-Val are separated by 495 nucleotides tRNA-Val and tRNA-Asp are separated by 3 nucleotides tRNA-Asp and tRNA-Asp are separated by 71 nucleotides
DVU0273: DVU0273 - conserved hypothetical protein (TIGR), at 315,149 to 316,666
DVU0273
DVU0274: DVU0274 - hypothetical protein (TIGR), at 316,978 to 317,349
DVU0274
DVU0275: DVU0275 - polysaccharide deacetylase family protein (TIGR), at 317,644 to 318,339
DVU0275
DVU_tRNA-Val-1: tRNA-Val - tRNA with anticodon CAC for Val (VIMSS-AUTO), at 318,835 to 318,910
tRNA-Val
DVU_tRNA-Asp-1: tRNA-Asp - tRNA with anticodon GTC for Asp (VIMSS-AUTO), at 318,914 to 318,990
tRNA-Asp
DVU_tRNA-Asp-2: tRNA-Asp - tRNA with anticodon GTC for Asp (VIMSS-AUTO), at 319,062 to 319,138
tRNA-Asp
Position (kb) 317
318
319 Strain fitness (log2 ratio) -2
-1
0
1
2 at 316.644 kb on - strand at 316.710 kb on - strand at 316.745 kb on - strand at 316.745 kb on - strand at 316.771 kb on - strand at 316.810 kb on + strand at 316.828 kb on - strand at 316.906 kb on - strand at 316.919 kb on + strand at 316.940 kb on + strand at 317.022 kb on + strand, within DVU0274 at 317.055 kb on - strand, within DVU0274 at 317.112 kb on - strand, within DVU0274 at 317.164 kb on - strand, within DVU0274 at 317.177 kb on + strand, within DVU0274 at 317.234 kb on + strand, within DVU0274 at 317.294 kb on - strand, within DVU0274 at 317.367 kb on + strand at 317.367 kb on + strand at 317.375 kb on - strand at 317.515 kb on + strand at 317.523 kb on - strand at 317.555 kb on + strand at 317.563 kb on - strand at 317.593 kb on + strand at 317.634 kb on - strand at 317.773 kb on + strand, within DVU0275 at 317.781 kb on + strand, within DVU0275 at 317.790 kb on - strand, within DVU0275 at 317.838 kb on + strand, within DVU0275 at 317.954 kb on + strand, within DVU0275 at 317.964 kb on + strand, within DVU0275 at 317.972 kb on - strand, within DVU0275 at 318.106 kb on + strand, within DVU0275 at 318.145 kb on - strand, within DVU0275 at 318.169 kb on + strand, within DVU0275 at 318.181 kb on + strand, within DVU0275 at 318.187 kb on - strand, within DVU0275 at 318.189 kb on - strand, within DVU0275 at 318.212 kb on + strand, within DVU0275 at 318.218 kb on + strand, within DVU0275 at 318.287 kb on + strand at 318.292 kb on + strand at 318.324 kb on - strand at 318.342 kb on - strand at 318.424 kb on + strand at 318.462 kb on - strand at 318.585 kb on + strand at 318.585 kb on + strand at 318.593 kb on - strand at 318.667 kb on + strand at 318.667 kb on + strand at 318.677 kb on + strand at 318.685 kb on - strand at 318.692 kb on + strand at 318.779 kb on + strand at 319.161 kb on + strand at 319.265 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glycine (N) remove 316,644 - -0.8 316,710 - -2.1 316,745 - +1.4 316,745 - +0.2 316,771 - +0.3 316,810 + -0.5 316,828 - +1.2 316,906 - -0.2 316,919 + +0.0 316,940 + -0.3 317,022 + DVU0274 0.12 -0.2 317,055 - DVU0274 0.21 -0.8 317,112 - DVU0274 0.36 -0.9 317,164 - DVU0274 0.50 +1.0 317,177 + DVU0274 0.53 +0.9 317,234 + DVU0274 0.69 +0.4 317,294 - DVU0274 0.85 -0.3 317,367 + -0.2 317,367 + +1.4 317,375 - -1.3 317,515 + -0.1 317,523 - -0.7 317,555 + -0.5 317,563 - -0.2 317,593 + +0.4 317,634 - -0.2 317,773 + DVU0275 0.19 -1.0 317,781 + DVU0275 0.20 +0.8 317,790 - DVU0275 0.21 +0.3 317,838 + DVU0275 0.28 -0.1 317,954 + DVU0275 0.45 -0.1 317,964 + DVU0275 0.46 -0.3 317,972 - DVU0275 0.47 +1.3 318,106 + DVU0275 0.66 +2.1 318,145 - DVU0275 0.72 -1.5 318,169 + DVU0275 0.75 -0.0 318,181 + DVU0275 0.77 +2.1 318,187 - DVU0275 0.78 -1.0 318,189 - DVU0275 0.78 +0.8 318,212 + DVU0275 0.82 +2.0 318,218 + DVU0275 0.82 +0.4 318,287 + +0.6 318,292 + -0.2 318,324 - -0.8 318,342 - +0.2 318,424 + +0.5 318,462 - +0.7 318,585 + +1.0 318,585 + +0.0 318,593 - -0.2 318,667 + +0.2 318,667 + -0.3 318,677 + -1.5 318,685 - -0.6 318,692 + -0.5 318,779 + +1.0 319,161 + -1.1 319,265 + -0.2
Or see this region's nucleotide sequence