Experiment: MoLS4 with L-Glycine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisI and hisG overlap by 4 nucleotides hisG and aroE are separated by 99 nucleotides
DVU0113: hisI - phosphoribosyl-AMP cyclohydrolase (TIGR), at 147,117 to 147,497
hisI
DVU0114: hisG - ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) , at 147,494 to 148,375
hisG
DVU0115: aroE - shikimate 5-dehydrogenase (TIGR), at 148,475 to 149,380
aroE
Position (kb)
147
148
149 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 146.506 kb on + strand at 146.542 kb on - strand at 146.547 kb on - strand at 146.563 kb on - strand at 146.575 kb on - strand at 146.612 kb on + strand at 146.627 kb on + strand at 146.627 kb on + strand at 146.652 kb on + strand at 146.660 kb on - strand at 146.704 kb on - strand at 146.704 kb on - strand at 146.708 kb on + strand at 146.739 kb on - strand at 146.796 kb on - strand at 146.881 kb on - strand at 146.882 kb on + strand at 146.883 kb on + strand at 146.912 kb on + strand at 146.912 kb on + strand at 146.912 kb on + strand at 146.920 kb on - strand at 146.939 kb on + strand at 146.939 kb on + strand at 146.947 kb on - strand at 146.948 kb on + strand at 146.956 kb on - strand at 146.971 kb on + strand at 146.971 kb on + strand at 147.098 kb on - strand at 147.215 kb on - strand, within hisI at 147.298 kb on - strand, within hisI at 147.304 kb on + strand, within hisI at 147.468 kb on - strand at 147.483 kb on - strand at 147.517 kb on - strand at 147.527 kb on + strand at 147.535 kb on - strand at 147.560 kb on - strand at 147.649 kb on - strand, within hisG at 147.680 kb on - strand, within hisG at 147.760 kb on + strand, within hisG at 147.821 kb on + strand, within hisG at 147.821 kb on + strand, within hisG at 147.828 kb on - strand, within hisG at 147.829 kb on - strand, within hisG at 147.829 kb on - strand, within hisG at 147.877 kb on - strand, within hisG at 147.894 kb on - strand, within hisG at 147.897 kb on - strand, within hisG at 147.946 kb on + strand, within hisG at 148.020 kb on - strand, within hisG at 148.071 kb on + strand, within hisG at 148.124 kb on - strand, within hisG at 148.172 kb on - strand, within hisG at 148.221 kb on + strand, within hisG at 148.280 kb on + strand, within hisG at 148.311 kb on + strand at 148.327 kb on + strand at 148.341 kb on - strand at 148.355 kb on + strand at 148.355 kb on + strand at 148.385 kb on + strand at 148.471 kb on + strand at 148.495 kb on - strand at 148.577 kb on + strand, within aroE at 149.321 kb on + strand at 149.321 kb on + strand at 149.321 kb on + strand at 149.322 kb on + strand at 149.324 kb on - strand at 149.329 kb on - strand at 149.374 kb on - strand at 149.375 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glycine (N) remove 146,506 + -1.5 146,542 - +0.8 146,547 - -1.2 146,563 - -0.5 146,575 - -0.3 146,612 + +0.3 146,627 + -0.4 146,627 + +0.2 146,652 + +0.2 146,660 - +1.1 146,704 - +0.5 146,704 - -0.4 146,708 + +0.5 146,739 - -0.6 146,796 - -1.1 146,881 - +0.0 146,882 + -0.5 146,883 + -1.1 146,912 + -0.3 146,912 + +0.0 146,912 + -0.2 146,920 - -0.1 146,939 + -0.4 146,939 + +0.0 146,947 - -1.5 146,948 + -0.4 146,956 - -0.5 146,971 + +0.7 146,971 + -0.1 147,098 - -0.3 147,215 - hisI DVU0113 0.26 -2.3 147,298 - hisI DVU0113 0.48 -5.0 147,304 + hisI DVU0113 0.49 +0.1 147,468 - -0.1 147,483 - -4.6 147,517 - -0.5 147,527 + -1.9 147,535 - -0.3 147,560 - -1.5 147,649 - hisG DVU0114 0.18 -1.7 147,680 - hisG DVU0114 0.21 -3.4 147,760 + hisG DVU0114 0.30 -0.1 147,821 + hisG DVU0114 0.37 -0.3 147,821 + hisG DVU0114 0.37 -1.6 147,828 - hisG DVU0114 0.38 -0.7 147,829 - hisG DVU0114 0.38 -1.5 147,829 - hisG DVU0114 0.38 -0.7 147,877 - hisG DVU0114 0.43 -1.4 147,894 - hisG DVU0114 0.45 -2.9 147,897 - hisG DVU0114 0.46 +0.4 147,946 + hisG DVU0114 0.51 -2.2 148,020 - hisG DVU0114 0.60 +0.7 148,071 + hisG DVU0114 0.65 -1.7 148,124 - hisG DVU0114 0.71 +0.1 148,172 - hisG DVU0114 0.77 -1.5 148,221 + hisG DVU0114 0.82 -0.6 148,280 + hisG DVU0114 0.89 -0.3 148,311 + +0.4 148,327 + +1.1 148,341 - +0.1 148,355 + -3.7 148,355 + -1.8 148,385 + -0.4 148,471 + -1.8 148,495 - -2.1 148,577 + aroE DVU0115 0.11 -0.9 149,321 + -4.7 149,321 + -3.0 149,321 + -1.4 149,322 + -1.1 149,324 - -1.4 149,329 - -2.0 149,374 - +0.7 149,375 - -0.6
Or see this region's nucleotide sequence