Experiment: MoLS4 with L-Glycine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU1459 and DVU1460 are separated by 35 nucleotides DVU1460 and hemA are separated by 17 nucleotides hemA and DVU1462 are separated by 1 nucleotides DVU1462 and cysG-1 are separated by 85 nucleotides
DVU1459: DVU1459 - conserved domain protein (TIGR), at 1,538,486 to 1,539,658
DVU1459
DVU1460: DVU1460 - hypothetical protein (TIGR), at 1,539,694 to 1,540,029
DVU1460
DVU1461: hemA - glutamyl-tRNA reductase (TIGR), at 1,540,047 to 1,541,369
hemA
DVU1462: DVU1462 - cytochrome c assembly protein, putative (TIGR), at 1,541,371 to 1,542,102
DVU1462
DVU1463: cysG-1 - siroheme synthase, N-terminal domain protein (TIGR), at 1,542,188 to 1,542,865
cysG-1
Position (kb) 1540
1541
1542 Strain fitness (log2 ratio) -2
-1
0
1 at 1539.758 kb on - strand, within DVU1460 at 1539.816 kb on - strand, within DVU1460 at 1540.035 kb on - strand at 1540.035 kb on - strand at 1541.570 kb on + strand, within DVU1462 at 1541.635 kb on + strand, within DVU1462 at 1541.635 kb on + strand, within DVU1462 at 1542.152 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Glycine (N) remove 1,539,758 - DVU1460 0.19 -1.5 1,539,816 - DVU1460 0.36 -0.3 1,540,035 - -0.6 1,540,035 - -0.6 1,541,570 + DVU1462 0.27 -2.2 1,541,635 + DVU1462 0.36 -0.1 1,541,635 + DVU1462 0.36 -1.5 1,542,152 + +1.3
Or see this region's nucleotide sequence