Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_974 and Pf1N1B4_975 are separated by 184 nucleotides Pf1N1B4_975 and Pf1N1B4_976 are separated by 3 nucleotides Pf1N1B4_976 and Pf1N1B4_977 overlap by 4 nucleotides Pf1N1B4_977 and Pf1N1B4_978 are separated by 234 nucleotides
Pf1N1B4_974 - Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation, at 1,103,342 to 1,103,851
_974
Pf1N1B4_975 - Molybdenum cofactor biosynthesis protein MoaE, at 1,104,036 to 1,104,488
_975
Pf1N1B4_976 - Molybdenum cofactor biosynthesis protein MoaD, at 1,104,492 to 1,104,734
_976
Pf1N1B4_977 - Molybdenum cofactor biosynthesis protein MoaC, at 1,104,731 to 1,105,204
_977
Pf1N1B4_978 - Predicted ATPase related to phosphate starvation-inducible protein PhoH, at 1,105,439 to 1,106,833
_978
Position (kb) 1104
1105
1106 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 1103.760 kb on + strand, within Pf1N1B4_974 at 1103.761 kb on - strand, within Pf1N1B4_974 at 1103.761 kb on - strand, within Pf1N1B4_974 at 1103.761 kb on - strand, within Pf1N1B4_974 at 1103.897 kb on - strand at 1103.897 kb on - strand at 1103.897 kb on - strand at 1103.897 kb on - strand at 1103.897 kb on - strand at 1103.897 kb on - strand at 1103.897 kb on - strand at 1103.897 kb on - strand at 1103.897 kb on - strand at 1103.978 kb on + strand at 1103.979 kb on - strand at 1104.000 kb on - strand at 1104.037 kb on + strand at 1104.038 kb on - strand at 1104.038 kb on - strand at 1104.038 kb on - strand at 1104.145 kb on + strand, within Pf1N1B4_975 at 1104.145 kb on + strand, within Pf1N1B4_975 at 1104.146 kb on - strand, within Pf1N1B4_975 at 1104.146 kb on - strand, within Pf1N1B4_975 at 1104.601 kb on - strand, within Pf1N1B4_976 at 1104.834 kb on + strand, within Pf1N1B4_977 at 1104.834 kb on + strand, within Pf1N1B4_977 at 1104.834 kb on + strand, within Pf1N1B4_977 at 1104.834 kb on + strand, within Pf1N1B4_977 at 1104.834 kb on + strand, within Pf1N1B4_977 at 1104.834 kb on + strand, within Pf1N1B4_977 at 1104.834 kb on + strand, within Pf1N1B4_977 at 1104.835 kb on - strand, within Pf1N1B4_977 at 1104.835 kb on - strand, within Pf1N1B4_977 at 1104.835 kb on - strand, within Pf1N1B4_977 at 1104.835 kb on - strand, within Pf1N1B4_977 at 1104.835 kb on - strand, within Pf1N1B4_977 at 1104.943 kb on + strand, within Pf1N1B4_977 at 1105.168 kb on + strand at 1105.168 kb on + strand at 1105.168 kb on + strand at 1105.168 kb on + strand at 1105.168 kb on + strand at 1105.168 kb on + strand at 1105.169 kb on - strand at 1105.210 kb on + strand at 1105.211 kb on - strand at 1105.235 kb on + strand at 1105.235 kb on + strand at 1105.268 kb on + strand at 1105.328 kb on + strand at 1105.335 kb on + strand at 1105.336 kb on - strand at 1105.380 kb on + strand at 1105.380 kb on + strand at 1105.380 kb on + strand at 1105.380 kb on + strand at 1105.380 kb on + strand at 1105.384 kb on + strand at 1105.384 kb on + strand at 1105.384 kb on + strand at 1105.384 kb on + strand at 1105.384 kb on + strand at 1105.384 kb on + strand at 1105.384 kb on + strand at 1105.385 kb on - strand at 1105.385 kb on - strand at 1105.385 kb on - strand at 1105.385 kb on - strand at 1105.385 kb on - strand at 1105.385 kb on - strand at 1105.385 kb on - strand at 1105.385 kb on - strand at 1105.394 kb on - strand at 1105.394 kb on - strand at 1105.502 kb on + strand at 1105.502 kb on + strand at 1105.502 kb on + strand at 1105.502 kb on + strand at 1105.502 kb on + strand at 1105.502 kb on + strand at 1105.502 kb on + strand at 1105.503 kb on - strand at 1105.503 kb on - strand at 1105.503 kb on - strand at 1105.503 kb on - strand at 1105.503 kb on - strand at 1105.503 kb on - strand at 1105.503 kb on - strand at 1105.539 kb on + strand at 1105.539 kb on + strand at 1105.539 kb on + strand at 1105.539 kb on + strand at 1105.539 kb on + strand at 1105.540 kb on - strand at 1105.540 kb on - strand at 1105.540 kb on - strand at 1105.540 kb on - strand at 1105.540 kb on - strand at 1105.540 kb on - strand at 1105.540 kb on - strand at 1105.569 kb on + strand at 1105.570 kb on - strand at 1105.660 kb on + strand, within Pf1N1B4_978 at 1105.660 kb on + strand, within Pf1N1B4_978 at 1105.661 kb on - strand, within Pf1N1B4_978 at 1105.719 kb on + strand, within Pf1N1B4_978 at 1105.719 kb on + strand, within Pf1N1B4_978 at 1105.719 kb on + strand, within Pf1N1B4_978 at 1105.719 kb on + strand, within Pf1N1B4_978 at 1105.719 kb on + strand, within Pf1N1B4_978 at 1105.719 kb on + strand, within Pf1N1B4_978 at 1105.719 kb on + strand, within Pf1N1B4_978 at 1105.719 kb on + strand, within Pf1N1B4_978 at 1105.719 kb on + strand, within Pf1N1B4_978 at 1105.719 kb on + strand, within Pf1N1B4_978 at 1105.720 kb on - strand, within Pf1N1B4_978 at 1105.720 kb on - strand, within Pf1N1B4_978 at 1105.720 kb on - strand, within Pf1N1B4_978 at 1105.720 kb on - strand at 1105.720 kb on - strand, within Pf1N1B4_978 at 1105.720 kb on - strand, within Pf1N1B4_978 at 1105.761 kb on + strand, within Pf1N1B4_978 at 1105.761 kb on + strand, within Pf1N1B4_978 at 1105.761 kb on + strand, within Pf1N1B4_978 at 1105.761 kb on + strand, within Pf1N1B4_978 at 1105.761 kb on + strand, within Pf1N1B4_978 at 1105.762 kb on - strand, within Pf1N1B4_978 at 1105.762 kb on - strand, within Pf1N1B4_978 at 1105.762 kb on - strand, within Pf1N1B4_978 at 1105.762 kb on - strand, within Pf1N1B4_978 at 1105.762 kb on - strand, within Pf1N1B4_978 at 1105.762 kb on - strand, within Pf1N1B4_978 at 1106.037 kb on + strand, within Pf1N1B4_978 at 1106.038 kb on - strand, within Pf1N1B4_978 at 1106.038 kb on - strand, within Pf1N1B4_978 at 1106.055 kb on + strand at 1106.055 kb on + strand, within Pf1N1B4_978 at 1106.055 kb on + strand, within Pf1N1B4_978 at 1106.055 kb on + strand, within Pf1N1B4_978 at 1106.056 kb on - strand, within Pf1N1B4_978 at 1106.056 kb on - strand, within Pf1N1B4_978
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 1,103,760 + Pf1N1B4_974 0.82 -0.0 1,103,761 - Pf1N1B4_974 0.82 -0.0 1,103,761 - Pf1N1B4_974 0.82 -0.0 1,103,761 - Pf1N1B4_974 0.82 +0.2 1,103,897 - -0.0 1,103,897 - -1.7 1,103,897 - -0.0 1,103,897 - -0.0 1,103,897 - -0.0 1,103,897 - -0.0 1,103,897 - -0.0 1,103,897 - -0.0 1,103,897 - -0.0 1,103,978 + -0.0 1,103,979 - -0.0 1,104,000 - -0.0 1,104,037 + -1.6 1,104,038 - -0.0 1,104,038 - -0.0 1,104,038 - -0.0 1,104,145 + Pf1N1B4_975 0.24 -1.3 1,104,145 + Pf1N1B4_975 0.24 -0.0 1,104,146 - Pf1N1B4_975 0.24 -0.0 1,104,146 - Pf1N1B4_975 0.24 -0.0 1,104,601 - Pf1N1B4_976 0.45 -1.7 1,104,834 + Pf1N1B4_977 0.22 -0.0 1,104,834 + Pf1N1B4_977 0.22 -6.1 1,104,834 + Pf1N1B4_977 0.22 -0.0 1,104,834 + Pf1N1B4_977 0.22 -4.8 1,104,834 + Pf1N1B4_977 0.22 -0.0 1,104,834 + Pf1N1B4_977 0.22 -0.0 1,104,834 + Pf1N1B4_977 0.22 -4.2 1,104,835 - Pf1N1B4_977 0.22 -5.4 1,104,835 - Pf1N1B4_977 0.22 -0.0 1,104,835 - Pf1N1B4_977 0.22 -0.0 1,104,835 - Pf1N1B4_977 0.22 -0.0 1,104,835 - Pf1N1B4_977 0.22 -0.0 1,104,943 + Pf1N1B4_977 0.45 -0.0 1,105,168 + -5.3 1,105,168 + -3.2 1,105,168 + -0.0 1,105,168 + -2.5 1,105,168 + -0.0 1,105,168 + -0.0 1,105,169 - -0.0 1,105,210 + -0.0 1,105,211 - -0.0 1,105,235 + -0.0 1,105,235 + -0.0 1,105,268 + -0.0 1,105,328 + -6.7 1,105,335 + -0.0 1,105,336 - -0.0 1,105,380 + -4.0 1,105,380 + -0.0 1,105,380 + -0.0 1,105,380 + -0.0 1,105,380 + -0.7 1,105,384 + -0.0 1,105,384 + -0.0 1,105,384 + -2.6 1,105,384 + -1.6 1,105,384 + -0.0 1,105,384 + -0.0 1,105,384 + -0.0 1,105,385 - -0.0 1,105,385 - -0.0 1,105,385 - -4.1 1,105,385 - -0.0 1,105,385 - -4.0 1,105,385 - -0.0 1,105,385 - -0.0 1,105,385 - -1.5 1,105,394 - -0.0 1,105,394 - -1.1 1,105,502 + -2.6 1,105,502 + -5.4 1,105,502 + -2.1 1,105,502 + -0.0 1,105,502 + -0.0 1,105,502 + -1.5 1,105,502 + -0.0 1,105,503 - -0.0 1,105,503 - -0.0 1,105,503 - -1.0 1,105,503 - -0.0 1,105,503 - -0.0 1,105,503 - -0.0 1,105,503 - -2.8 1,105,539 + -1.3 1,105,539 + -0.0 1,105,539 + -0.0 1,105,539 + -0.8 1,105,539 + -0.0 1,105,540 - -0.0 1,105,540 - -2.1 1,105,540 - -0.0 1,105,540 - -0.0 1,105,540 - -0.0 1,105,540 - -0.0 1,105,540 - -0.1 1,105,569 + -0.0 1,105,570 - -0.0 1,105,660 + Pf1N1B4_978 0.16 -0.0 1,105,660 + Pf1N1B4_978 0.16 -1.3 1,105,661 - Pf1N1B4_978 0.16 -0.0 1,105,719 + Pf1N1B4_978 0.20 -5.1 1,105,719 + Pf1N1B4_978 0.20 -0.0 1,105,719 + Pf1N1B4_978 0.20 -1.0 1,105,719 + Pf1N1B4_978 0.20 -0.0 1,105,719 + Pf1N1B4_978 0.20 -0.0 1,105,719 + Pf1N1B4_978 0.20 -1.8 1,105,719 + Pf1N1B4_978 0.20 -1.6 1,105,719 + Pf1N1B4_978 0.20 -1.9 1,105,719 + Pf1N1B4_978 0.20 -0.0 1,105,719 + Pf1N1B4_978 0.20 -2.1 1,105,720 - Pf1N1B4_978 0.20 -4.8 1,105,720 - Pf1N1B4_978 0.20 -0.0 1,105,720 - Pf1N1B4_978 0.20 -0.0 1,105,720 - -0.0 1,105,720 - Pf1N1B4_978 0.20 -0.0 1,105,720 - Pf1N1B4_978 0.20 -2.3 1,105,761 + Pf1N1B4_978 0.23 -0.0 1,105,761 + Pf1N1B4_978 0.23 -0.0 1,105,761 + Pf1N1B4_978 0.23 -0.0 1,105,761 + Pf1N1B4_978 0.23 -0.0 1,105,761 + Pf1N1B4_978 0.23 -0.0 1,105,762 - Pf1N1B4_978 0.23 -0.0 1,105,762 - Pf1N1B4_978 0.23 -0.0 1,105,762 - Pf1N1B4_978 0.23 -1.1 1,105,762 - Pf1N1B4_978 0.23 -1.5 1,105,762 - Pf1N1B4_978 0.23 -0.0 1,105,762 - Pf1N1B4_978 0.23 -0.0 1,106,037 + Pf1N1B4_978 0.43 -2.3 1,106,038 - Pf1N1B4_978 0.43 -0.0 1,106,038 - Pf1N1B4_978 0.43 -0.0 1,106,055 + -0.3 1,106,055 + Pf1N1B4_978 0.44 -0.0 1,106,055 + Pf1N1B4_978 0.44 -0.0 1,106,055 + Pf1N1B4_978 0.44 -0.7 1,106,056 - Pf1N1B4_978 0.44 -0.0 1,106,056 - Pf1N1B4_978 0.44 -5.5
Or see this region's nucleotide sequence