Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_733

Experiment: Nitrate reduction

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_731 and Pf1N1B4_732 are separated by 55 nucleotidesPf1N1B4_732 and Pf1N1B4_733 are separated by 108 nucleotidesPf1N1B4_733 and Pf1N1B4_734 are separated by 129 nucleotidesPf1N1B4_734 and Pf1N1B4_735 overlap by 4 nucleotides Pf1N1B4_731 - Scaffold protein for [4Fe-4S] cluster assembly ApbC, MRP-like, at 833,953 to 835,047 _731 Pf1N1B4_732 - hypothetical protein, at 835,103 to 835,306 _732 Pf1N1B4_733 - Methionyl-tRNA synthetase (EC 6.1.1.10), at 835,415 to 837,466 _733 Pf1N1B4_734 - Electron transport complex protein RnfA, at 837,596 to 838,165 _734 Pf1N1B4_735 - Electron transport complex protein RnfB, at 838,162 to 839,169 _735 Position (kb) 835 836 837 838Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 834.903 kb on - strand, within Pf1N1B4_731at 834.903 kb on - strand, within Pf1N1B4_731at 834.903 kb on - strand, within Pf1N1B4_731at 835.090 kb on + strandat 835.099 kb on + strandat 835.099 kb on + strandat 835.099 kb on + strandat 835.099 kb on + strandat 835.099 kb on + strandat 835.099 kb on + strandat 835.099 kb on + strandat 835.099 kb on + strandat 835.099 kb on + strandat 835.100 kb on - strandat 835.100 kb on - strandat 835.100 kb on - strandat 835.100 kb on - strandat 835.100 kb on - strandat 835.100 kb on - strandat 835.100 kb on - strandat 835.100 kb on - strandat 835.106 kb on + strandat 835.106 kb on + strandat 835.106 kb on + strandat 835.106 kb on + strandat 835.106 kb on + strandat 835.107 kb on - strandat 835.107 kb on - strandat 835.107 kb on - strandat 835.107 kb on - strandat 835.107 kb on - strandat 835.107 kb on - strandat 835.107 kb on - strandat 835.189 kb on + strand, within Pf1N1B4_732at 837.030 kb on + strand, within Pf1N1B4_733at 837.030 kb on + strand, within Pf1N1B4_733at 837.030 kb on + strand, within Pf1N1B4_733at 837.465 kb on - strandat 837.465 kb on - strandat 837.558 kb on + strandat 837.758 kb on + strand, within Pf1N1B4_734at 837.768 kb on - strand, within Pf1N1B4_734at 837.777 kb on + strand, within Pf1N1B4_734at 837.778 kb on - strand, within Pf1N1B4_734at 837.778 kb on - strand, within Pf1N1B4_734at 837.780 kb on + strand, within Pf1N1B4_734at 837.780 kb on + strand, within Pf1N1B4_734at 837.781 kb on - strand, within Pf1N1B4_734at 837.984 kb on + strand, within Pf1N1B4_734at 838.233 kb on + strandat 838.233 kb on + strandat 838.233 kb on + strandat 838.233 kb on + strandat 838.234 kb on - strandat 838.234 kb on - strandat 838.234 kb on - strandat 838.234 kb on - strandat 838.234 kb on - strandat 838.234 kb on - strandat 838.234 kb on - strandat 838.234 kb on - strandat 838.241 kb on - strandat 838.241 kb on - strandat 838.384 kb on + strand, within Pf1N1B4_735at 838.385 kb on - strand, within Pf1N1B4_735at 838.385 kb on - strand, within Pf1N1B4_735

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction
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834,903 - Pf1N1B4_731 0.87 -0.0
834,903 - Pf1N1B4_731 0.87 -0.0
834,903 - Pf1N1B4_731 0.87 -0.0
835,090 + -0.0
835,099 + -0.5
835,099 + -2.6
835,099 + -0.0
835,099 + -2.3
835,099 + -0.0
835,099 + -1.4
835,099 + -0.0
835,099 + -0.0
835,099 + -0.0
835,100 - -0.0
835,100 - -0.0
835,100 - -2.2
835,100 - -0.0
835,100 - -0.0
835,100 - -0.0
835,100 - -1.0
835,100 - -0.0
835,106 + -2.6
835,106 + -1.6
835,106 + -5.0
835,106 + -2.0
835,106 + -1.6
835,107 - -0.0
835,107 - -0.0
835,107 - -4.7
835,107 - -4.0
835,107 - -0.0
835,107 - -0.0
835,107 - -0.0
835,189 + Pf1N1B4_732 0.42 -1.3
837,030 + Pf1N1B4_733 0.79 -0.0
837,030 + Pf1N1B4_733 0.79 -0.0
837,030 + Pf1N1B4_733 0.79 -3.2
837,465 - -0.0
837,465 - -0.0
837,558 + -1.3
837,758 + Pf1N1B4_734 0.28 -0.0
837,768 - Pf1N1B4_734 0.30 -0.0
837,777 + Pf1N1B4_734 0.32 -0.0
837,778 - Pf1N1B4_734 0.32 -0.0
837,778 - Pf1N1B4_734 0.32 -1.8
837,780 + Pf1N1B4_734 0.32 -0.0
837,780 + Pf1N1B4_734 0.32 -0.0
837,781 - Pf1N1B4_734 0.32 -0.0
837,984 + Pf1N1B4_734 0.68 -2.9
838,233 + -1.8
838,233 + -0.0
838,233 + -0.0
838,233 + -0.0
838,234 - -4.1
838,234 - -0.0
838,234 - -0.0
838,234 - -0.0
838,234 - -0.0
838,234 - -3.3
838,234 - -4.7
838,234 - -3.6
838,241 - -0.0
838,241 - -0.0
838,384 + Pf1N1B4_735 0.22 -0.0
838,385 - Pf1N1B4_735 0.22 -0.0
838,385 - Pf1N1B4_735 0.22 -0.0

Or see this region's nucleotide sequence