Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5961 and Pf1N1B4_5962 overlap by 42 nucleotides Pf1N1B4_5962 and Pf1N1B4_5963 are separated by 233 nucleotides
Pf1N1B4_5961 - Major facilitator superfamily transporter, at 409,546 to 411,222
_5961
Pf1N1B4_5962 - hypothetical protein, at 411,181 to 411,342
_5962
Pf1N1B4_5963 - Predicted regulator PutR for proline utilization, GntR family, at 411,576 to 412,247
_5963
Position (kb) 411
412 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 410.201 kb on + strand, within Pf1N1B4_5961 at 410.232 kb on - strand, within Pf1N1B4_5961 at 410.248 kb on + strand, within Pf1N1B4_5961 at 410.249 kb on - strand, within Pf1N1B4_5961 at 410.405 kb on + strand, within Pf1N1B4_5961 at 410.420 kb on + strand, within Pf1N1B4_5961 at 410.470 kb on + strand, within Pf1N1B4_5961 at 410.582 kb on + strand at 410.777 kb on + strand, within Pf1N1B4_5961 at 410.777 kb on + strand, within Pf1N1B4_5961 at 410.778 kb on - strand at 410.778 kb on - strand, within Pf1N1B4_5961 at 410.778 kb on - strand, within Pf1N1B4_5961 at 410.778 kb on - strand, within Pf1N1B4_5961 at 410.778 kb on - strand, within Pf1N1B4_5961 at 410.937 kb on + strand, within Pf1N1B4_5961 at 410.937 kb on + strand, within Pf1N1B4_5961 at 410.937 kb on + strand, within Pf1N1B4_5961 at 410.938 kb on - strand at 410.938 kb on - strand, within Pf1N1B4_5961 at 410.938 kb on - strand at 410.938 kb on - strand, within Pf1N1B4_5961 at 410.938 kb on - strand, within Pf1N1B4_5961 at 410.938 kb on - strand, within Pf1N1B4_5961 at 410.996 kb on - strand, within Pf1N1B4_5961 at 410.996 kb on - strand, within Pf1N1B4_5961 at 411.205 kb on - strand at 411.248 kb on - strand, within Pf1N1B4_5962 at 411.250 kb on - strand, within Pf1N1B4_5962 at 411.250 kb on - strand, within Pf1N1B4_5962 at 411.250 kb on - strand, within Pf1N1B4_5962 at 411.250 kb on - strand, within Pf1N1B4_5962 at 411.268 kb on - strand, within Pf1N1B4_5962 at 411.394 kb on + strand at 411.435 kb on - strand at 411.490 kb on - strand at 411.490 kb on - strand at 411.614 kb on + strand at 411.614 kb on + strand at 411.877 kb on + strand, within Pf1N1B4_5963 at 411.878 kb on - strand, within Pf1N1B4_5963 at 412.062 kb on - strand, within Pf1N1B4_5963 at 412.281 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 410,201 + Pf1N1B4_5961 0.39 -1.7 410,232 - Pf1N1B4_5961 0.41 -0.0 410,248 + Pf1N1B4_5961 0.42 -4.2 410,249 - Pf1N1B4_5961 0.42 -0.0 410,405 + Pf1N1B4_5961 0.51 -2.6 410,420 + Pf1N1B4_5961 0.52 -0.0 410,470 + Pf1N1B4_5961 0.55 -2.3 410,582 + -0.0 410,777 + Pf1N1B4_5961 0.73 -0.0 410,777 + Pf1N1B4_5961 0.73 -0.0 410,778 - -0.0 410,778 - Pf1N1B4_5961 0.73 -0.0 410,778 - Pf1N1B4_5961 0.73 -5.0 410,778 - Pf1N1B4_5961 0.73 -0.0 410,778 - Pf1N1B4_5961 0.73 -1.2 410,937 + Pf1N1B4_5961 0.83 -0.9 410,937 + Pf1N1B4_5961 0.83 -0.0 410,937 + Pf1N1B4_5961 0.83 -0.0 410,938 - -0.0 410,938 - Pf1N1B4_5961 0.83 -0.0 410,938 - -0.0 410,938 - Pf1N1B4_5961 0.83 -0.0 410,938 - Pf1N1B4_5961 0.83 -1.4 410,938 - Pf1N1B4_5961 0.83 -4.4 410,996 - Pf1N1B4_5961 0.86 -1.5 410,996 - Pf1N1B4_5961 0.86 -1.7 411,205 - -0.0 411,248 - Pf1N1B4_5962 0.41 -2.1 411,250 - Pf1N1B4_5962 0.43 -1.0 411,250 - Pf1N1B4_5962 0.43 -5.1 411,250 - Pf1N1B4_5962 0.43 -1.9 411,250 - Pf1N1B4_5962 0.43 -4.6 411,268 - Pf1N1B4_5962 0.54 -0.0 411,394 + -0.0 411,435 - -5.7 411,490 - -0.0 411,490 - -0.0 411,614 + -1.9 411,614 + -0.0 411,877 + Pf1N1B4_5963 0.45 -0.0 411,878 - Pf1N1B4_5963 0.45 -0.0 412,062 - Pf1N1B4_5963 0.72 -0.0 412,281 - -3.2
Or see this region's nucleotide sequence