Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5777 and Pf1N1B4_5778 are separated by 31 nucleotides Pf1N1B4_5778 and Pf1N1B4_5779 overlap by 4 nucleotides Pf1N1B4_5779 and Pf1N1B4_5780 overlap by 4 nucleotides
Pf1N1B4_5777 - Nitrous oxide reductase maturation protein, outer-membrane lipoprotein NosL, at 214,238 to 214,765
_5777
Pf1N1B4_5778 - Nitrous oxide reductase maturation transmembrane protein NosY, at 214,797 to 215,627
_5778
Pf1N1B4_5779 - Nitrous oxide reductase maturation protein NosF (ATPase), at 215,624 to 216,547
_5779
Pf1N1B4_5780 - Nitrous oxide reductase maturation protein NosD, at 216,544 to 217,836
_5780
Position (kb) 215
216
217 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 214.718 kb on + strand at 214.719 kb on - strand at 214.760 kb on - strand at 214.760 kb on - strand at 214.986 kb on + strand, within Pf1N1B4_5778 at 215.143 kb on + strand, within Pf1N1B4_5778 at 215.668 kb on - strand at 215.668 kb on - strand at 215.676 kb on + strand at 215.735 kb on + strand, within Pf1N1B4_5779 at 215.736 kb on - strand, within Pf1N1B4_5779 at 215.987 kb on + strand, within Pf1N1B4_5779 at 215.987 kb on + strand at 215.988 kb on - strand, within Pf1N1B4_5779 at 215.988 kb on - strand at 216.291 kb on + strand, within Pf1N1B4_5779 at 216.505 kb on - strand at 216.510 kb on + strand at 216.738 kb on - strand at 216.842 kb on + strand at 216.843 kb on - strand, within Pf1N1B4_5780 at 216.975 kb on - strand, within Pf1N1B4_5780 at 217.070 kb on + strand, within Pf1N1B4_5780 at 217.079 kb on + strand, within Pf1N1B4_5780 at 217.080 kb on - strand, within Pf1N1B4_5780 at 217.220 kb on + strand, within Pf1N1B4_5780 at 217.220 kb on + strand, within Pf1N1B4_5780 at 217.220 kb on + strand, within Pf1N1B4_5780 at 217.220 kb on + strand, within Pf1N1B4_5780 at 217.220 kb on + strand, within Pf1N1B4_5780 at 217.220 kb on + strand, within Pf1N1B4_5780 at 217.221 kb on - strand at 217.289 kb on + strand, within Pf1N1B4_5780 at 217.289 kb on + strand, within Pf1N1B4_5780 at 217.349 kb on + strand, within Pf1N1B4_5780
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 214,718 + -2.8 214,719 - -0.0 214,760 - -0.0 214,760 - -4.9 214,986 + Pf1N1B4_5778 0.23 -0.0 215,143 + Pf1N1B4_5778 0.42 -0.0 215,668 - -0.0 215,668 - -5.8 215,676 + -2.2 215,735 + Pf1N1B4_5779 0.12 -0.0 215,736 - Pf1N1B4_5779 0.12 -4.4 215,987 + Pf1N1B4_5779 0.39 -0.0 215,987 + -0.0 215,988 - Pf1N1B4_5779 0.39 -0.0 215,988 - -1.2 216,291 + Pf1N1B4_5779 0.72 -0.0 216,505 - -0.0 216,510 + -0.0 216,738 - -0.9 216,842 + -0.0 216,843 - Pf1N1B4_5780 0.23 -0.0 216,975 - Pf1N1B4_5780 0.33 -2.3 217,070 + Pf1N1B4_5780 0.41 -2.3 217,079 + Pf1N1B4_5780 0.41 -0.0 217,080 - Pf1N1B4_5780 0.41 -0.0 217,220 + Pf1N1B4_5780 0.52 -0.0 217,220 + Pf1N1B4_5780 0.52 -2.3 217,220 + Pf1N1B4_5780 0.52 -0.8 217,220 + Pf1N1B4_5780 0.52 -0.0 217,220 + Pf1N1B4_5780 0.52 -2.3 217,220 + Pf1N1B4_5780 0.52 -1.3 217,221 - -0.0 217,289 + Pf1N1B4_5780 0.58 -0.0 217,289 + Pf1N1B4_5780 0.58 -3.3 217,349 + Pf1N1B4_5780 0.62 -0.0
Or see this region's nucleotide sequence