Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5738 and Pf1N1B4_5739 overlap by 24 nucleotides Pf1N1B4_5739 and Pf1N1B4_5740 are separated by 135 nucleotides Pf1N1B4_5740 and Pf1N1B4_5741 overlap by 4 nucleotides Pf1N1B4_5741 and Pf1N1B4_5742 are separated by 86 nucleotides
Pf1N1B4_5738 - Nitrate/nitrite transporter, at 180,523 to 181,347
_5738
Pf1N1B4_5739 - hypothetical protein, at 181,324 to 181,515
_5739
Pf1N1B4_5740 - Nitrate/nitrite sensor protein (EC 2.7.3.-), at 181,651 to 183,453
_5740
Pf1N1B4_5741 - Nitrate/nitrite response regulator protein, at 183,450 to 184,097
_5741
Pf1N1B4_5742 - Nitric oxide -responding transcriptional regulator Dnr (Crp/Fnr family), at 184,184 to 184,858
_5742
Position (kb) 181
182
183
184 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 180.651 kb on - strand, within Pf1N1B4_5738 at 180.651 kb on - strand at 180.651 kb on - strand, within Pf1N1B4_5738 at 180.704 kb on + strand, within Pf1N1B4_5738 at 180.705 kb on - strand, within Pf1N1B4_5738 at 180.986 kb on + strand, within Pf1N1B4_5738 at 180.986 kb on + strand, within Pf1N1B4_5738 at 180.987 kb on - strand, within Pf1N1B4_5738 at 180.987 kb on - strand, within Pf1N1B4_5738 at 180.987 kb on - strand, within Pf1N1B4_5738 at 181.331 kb on - strand at 181.331 kb on - strand at 181.331 kb on - strand at 181.382 kb on + strand, within Pf1N1B4_5739 at 181.444 kb on - strand, within Pf1N1B4_5739 at 181.564 kb on - strand at 181.564 kb on - strand at 181.958 kb on - strand, within Pf1N1B4_5740 at 181.958 kb on - strand, within Pf1N1B4_5740 at 181.958 kb on - strand, within Pf1N1B4_5740 at 181.992 kb on - strand, within Pf1N1B4_5740 at 182.181 kb on - strand, within Pf1N1B4_5740 at 182.185 kb on + strand, within Pf1N1B4_5740 at 182.810 kb on + strand, within Pf1N1B4_5740 at 182.876 kb on - strand at 182.899 kb on - strand, within Pf1N1B4_5740 at 182.984 kb on - strand, within Pf1N1B4_5740 at 183.222 kb on - strand, within Pf1N1B4_5740 at 183.805 kb on - strand, within Pf1N1B4_5741 at 184.134 kb on + strand at 184.134 kb on + strand at 184.134 kb on + strand at 184.160 kb on + strand at 184.160 kb on + strand at 184.161 kb on - strand at 184.179 kb on - strand at 184.210 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 180,651 - Pf1N1B4_5738 0.16 -0.0 180,651 - -3.0 180,651 - Pf1N1B4_5738 0.16 -0.0 180,704 + Pf1N1B4_5738 0.22 -1.1 180,705 - Pf1N1B4_5738 0.22 -0.0 180,986 + Pf1N1B4_5738 0.56 -2.1 180,986 + Pf1N1B4_5738 0.56 -0.0 180,987 - Pf1N1B4_5738 0.56 -5.2 180,987 - Pf1N1B4_5738 0.56 -0.0 180,987 - Pf1N1B4_5738 0.56 -0.0 181,331 - -0.0 181,331 - -4.7 181,331 - -2.0 181,382 + Pf1N1B4_5739 0.30 -0.0 181,444 - Pf1N1B4_5739 0.62 -3.6 181,564 - -3.3 181,564 - -0.0 181,958 - Pf1N1B4_5740 0.17 -0.0 181,958 - Pf1N1B4_5740 0.17 -0.0 181,958 - Pf1N1B4_5740 0.17 -3.2 181,992 - Pf1N1B4_5740 0.19 -4.6 182,181 - Pf1N1B4_5740 0.29 -0.0 182,185 + Pf1N1B4_5740 0.30 -0.0 182,810 + Pf1N1B4_5740 0.64 -2.6 182,876 - -0.0 182,899 - Pf1N1B4_5740 0.69 -0.0 182,984 - Pf1N1B4_5740 0.74 -0.0 183,222 - Pf1N1B4_5740 0.87 -2.8 183,805 - Pf1N1B4_5741 0.55 -5.1 184,134 + -0.0 184,134 + -0.0 184,134 + -0.0 184,160 + -0.0 184,160 + -0.0 184,161 - -3.1 184,179 - -0.0 184,210 - -0.0
Or see this region's nucleotide sequence