Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5542 and Pf1N1B4_5543 are separated by 370 nucleotides Pf1N1B4_5543 and Pf1N1B4_5544 are separated by 10 nucleotides Pf1N1B4_5544 and Pf1N1B4_5545 are separated by 777 nucleotides
Pf1N1B4_5542 - hypothetical protein, at 308,320 to 308,523
_5542
Pf1N1B4_5543 - hypothetical protein, at 308,894 to 309,010
_5543
Pf1N1B4_5544 - Methyl-accepting chemotaxis protein, at 309,021 to 311,015
_5544
Pf1N1B4_5545 - Signal transduction histidine kinase CheA (EC 2.7.3.-), at 311,793 to 317,519
_5545
Position (kb) 309
310
311
312 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 308.061 kb on - strand at 308.113 kb on + strand at 308.114 kb on - strand at 308.114 kb on - strand at 308.117 kb on + strand at 308.118 kb on - strand at 308.118 kb on - strand at 308.118 kb on - strand at 308.118 kb on - strand at 308.118 kb on - strand at 308.118 kb on - strand at 308.118 kb on - strand at 308.122 kb on - strand at 308.122 kb on - strand at 308.129 kb on - strand at 308.129 kb on - strand at 308.152 kb on - strand at 308.152 kb on - strand at 308.152 kb on - strand at 308.252 kb on - strand at 308.366 kb on - strand, within Pf1N1B4_5542 at 308.366 kb on - strand, within Pf1N1B4_5542 at 308.449 kb on + strand, within Pf1N1B4_5542 at 308.450 kb on - strand, within Pf1N1B4_5542 at 308.521 kb on + strand at 308.522 kb on - strand at 308.522 kb on - strand at 308.574 kb on - strand at 308.574 kb on - strand at 308.637 kb on - strand at 308.695 kb on - strand at 308.695 kb on - strand at 308.702 kb on + strand at 308.762 kb on - strand at 308.762 kb on - strand at 308.762 kb on - strand at 308.762 kb on - strand at 308.848 kb on + strand at 308.848 kb on + strand at 308.848 kb on + strand at 308.849 kb on - strand at 308.849 kb on - strand at 308.849 kb on - strand at 308.850 kb on + strand at 308.850 kb on + strand at 308.850 kb on + strand at 308.850 kb on + strand at 308.851 kb on - strand at 308.851 kb on - strand at 308.851 kb on - strand at 308.851 kb on - strand at 308.851 kb on - strand at 308.851 kb on - strand at 308.963 kb on + strand, within Pf1N1B4_5543 at 308.964 kb on - strand, within Pf1N1B4_5543 at 308.964 kb on - strand at 308.993 kb on - strand, within Pf1N1B4_5543 at 309.004 kb on - strand at 309.004 kb on - strand at 309.004 kb on - strand at 309.004 kb on - strand at 309.036 kb on + strand at 309.037 kb on - strand at 309.037 kb on - strand at 309.037 kb on - strand at 309.037 kb on - strand at 309.037 kb on - strand at 309.037 kb on - strand at 309.180 kb on + strand at 309.180 kb on + strand at 309.180 kb on + strand at 309.181 kb on - strand at 309.182 kb on + strand at 309.182 kb on + strand at 309.182 kb on + strand at 309.182 kb on + strand at 309.183 kb on - strand at 309.183 kb on - strand at 309.213 kb on + strand at 309.213 kb on + strand at 309.214 kb on - strand at 309.248 kb on - strand, within Pf1N1B4_5544 at 309.330 kb on - strand, within Pf1N1B4_5544 at 309.344 kb on + strand, within Pf1N1B4_5544 at 309.359 kb on - strand at 309.359 kb on - strand at 309.398 kb on + strand, within Pf1N1B4_5544 at 309.398 kb on + strand, within Pf1N1B4_5544 at 309.410 kb on + strand, within Pf1N1B4_5544 at 309.421 kb on + strand, within Pf1N1B4_5544 at 309.421 kb on + strand, within Pf1N1B4_5544 at 309.422 kb on - strand at 309.565 kb on + strand, within Pf1N1B4_5544 at 309.565 kb on + strand, within Pf1N1B4_5544 at 309.569 kb on + strand, within Pf1N1B4_5544 at 309.570 kb on - strand, within Pf1N1B4_5544 at 309.630 kb on + strand, within Pf1N1B4_5544 at 309.631 kb on - strand, within Pf1N1B4_5544 at 309.824 kb on + strand, within Pf1N1B4_5544 at 309.907 kb on + strand, within Pf1N1B4_5544 at 309.907 kb on + strand, within Pf1N1B4_5544 at 309.907 kb on + strand at 309.908 kb on - strand, within Pf1N1B4_5544 at 309.908 kb on - strand at 309.911 kb on + strand, within Pf1N1B4_5544 at 309.911 kb on + strand, within Pf1N1B4_5544 at 309.911 kb on + strand, within Pf1N1B4_5544 at 309.912 kb on - strand, within Pf1N1B4_5544 at 309.912 kb on - strand, within Pf1N1B4_5544 at 309.985 kb on + strand, within Pf1N1B4_5544 at 309.985 kb on + strand at 309.986 kb on - strand, within Pf1N1B4_5544 at 310.018 kb on + strand, within Pf1N1B4_5544 at 310.019 kb on - strand, within Pf1N1B4_5544 at 310.040 kb on + strand, within Pf1N1B4_5544 at 310.163 kb on + strand, within Pf1N1B4_5544 at 310.164 kb on - strand, within Pf1N1B4_5544 at 310.181 kb on + strand, within Pf1N1B4_5544 at 310.182 kb on - strand, within Pf1N1B4_5544 at 310.182 kb on - strand, within Pf1N1B4_5544 at 310.341 kb on - strand, within Pf1N1B4_5544 at 310.341 kb on - strand, within Pf1N1B4_5544 at 310.372 kb on + strand, within Pf1N1B4_5544 at 310.373 kb on - strand, within Pf1N1B4_5544 at 310.373 kb on - strand, within Pf1N1B4_5544 at 310.373 kb on - strand, within Pf1N1B4_5544 at 310.373 kb on - strand, within Pf1N1B4_5544 at 310.375 kb on + strand at 310.375 kb on + strand, within Pf1N1B4_5544 at 310.376 kb on - strand at 310.418 kb on + strand, within Pf1N1B4_5544 at 310.554 kb on + strand, within Pf1N1B4_5544 at 310.555 kb on - strand, within Pf1N1B4_5544 at 310.643 kb on + strand, within Pf1N1B4_5544 at 310.643 kb on + strand, within Pf1N1B4_5544 at 310.643 kb on + strand, within Pf1N1B4_5544 at 310.644 kb on - strand, within Pf1N1B4_5544 at 310.751 kb on + strand, within Pf1N1B4_5544 at 310.752 kb on - strand, within Pf1N1B4_5544 at 310.810 kb on + strand, within Pf1N1B4_5544 at 310.811 kb on - strand, within Pf1N1B4_5544 at 310.823 kb on - strand at 310.902 kb on + strand at 310.946 kb on + strand at 310.947 kb on - strand at 311.014 kb on - strand at 311.014 kb on - strand at 311.041 kb on + strand at 311.116 kb on + strand at 311.116 kb on + strand at 311.142 kb on + strand at 311.158 kb on + strand at 311.191 kb on + strand at 311.191 kb on + strand at 311.191 kb on + strand at 311.191 kb on + strand at 311.192 kb on - strand at 311.192 kb on - strand at 311.192 kb on - strand at 311.230 kb on - strand at 311.261 kb on + strand at 311.262 kb on - strand at 311.262 kb on - strand at 311.354 kb on + strand at 311.354 kb on + strand at 311.354 kb on + strand at 311.354 kb on + strand at 311.355 kb on - strand at 311.524 kb on + strand at 311.524 kb on + strand at 311.525 kb on - strand at 311.525 kb on - strand at 311.545 kb on + strand at 311.545 kb on + strand at 311.545 kb on + strand at 311.546 kb on - strand at 311.607 kb on + strand at 311.607 kb on + strand at 311.607 kb on + strand at 311.607 kb on + strand at 311.607 kb on + strand at 311.608 kb on - strand at 311.608 kb on - strand at 311.608 kb on - strand at 311.630 kb on + strand at 311.630 kb on + strand at 311.630 kb on + strand at 311.631 kb on - strand at 311.631 kb on - strand at 311.631 kb on - strand at 311.631 kb on - strand at 311.631 kb on - strand at 311.634 kb on - strand at 311.742 kb on + strand at 311.743 kb on - strand at 311.743 kb on - strand at 311.746 kb on + strand at 311.746 kb on + strand at 311.747 kb on - strand at 311.843 kb on + strand at 311.844 kb on - strand at 311.881 kb on - strand at 312.015 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 308,061 - +0.0 308,113 + +0.0 308,114 - +0.0 308,114 - +0.0 308,117 + +0.0 308,118 - -1.9 308,118 - +0.0 308,118 - +0.0 308,118 - +0.0 308,118 - +0.0 308,118 - +0.0 308,118 - +0.0 308,122 - +0.0 308,122 - -1.3 308,129 - +0.0 308,129 - +0.0 308,152 - +0.0 308,152 - +0.0 308,152 - +0.0 308,252 - -5.3 308,366 - Pf1N1B4_5542 0.23 -3.8 308,366 - Pf1N1B4_5542 0.23 +0.0 308,449 + Pf1N1B4_5542 0.63 +0.0 308,450 - Pf1N1B4_5542 0.64 +0.0 308,521 + -1.3 308,522 - +0.0 308,522 - -1.4 308,574 - +0.0 308,574 - -4.7 308,637 - +0.0 308,695 - -1.5 308,695 - +0.0 308,702 + +0.0 308,762 - -3.6 308,762 - +0.0 308,762 - -1.5 308,762 - -1.2 308,848 + +0.0 308,848 + +0.0 308,848 + +0.0 308,849 - +0.0 308,849 - +0.0 308,849 - +0.0 308,850 + -0.9 308,850 + +0.0 308,850 + -2.1 308,850 + +0.0 308,851 - -0.8 308,851 - +0.0 308,851 - -4.1 308,851 - +0.0 308,851 - +0.0 308,851 - +0.0 308,963 + Pf1N1B4_5543 0.59 +0.0 308,964 - Pf1N1B4_5543 0.60 -2.1 308,964 - +0.0 308,993 - Pf1N1B4_5543 0.85 +0.0 309,004 - +0.0 309,004 - +0.0 309,004 - -1.0 309,004 - +0.0 309,036 + +0.0 309,037 - +0.0 309,037 - +0.0 309,037 - +0.0 309,037 - -3.1 309,037 - +0.0 309,037 - +0.0 309,180 + +0.0 309,180 + -5.9 309,180 + +0.5 309,181 - +0.0 309,182 + +0.0 309,182 + +0.0 309,182 + +0.0 309,182 + +0.0 309,183 - -2.2 309,183 - +0.0 309,213 + +0.0 309,213 + -1.5 309,214 - +0.0 309,248 - Pf1N1B4_5544 0.11 +0.0 309,330 - Pf1N1B4_5544 0.15 +0.0 309,344 + Pf1N1B4_5544 0.16 +0.0 309,359 - -2.5 309,359 - -1.6 309,398 + Pf1N1B4_5544 0.19 +0.0 309,398 + Pf1N1B4_5544 0.19 +0.0 309,410 + Pf1N1B4_5544 0.19 -0.8 309,421 + Pf1N1B4_5544 0.20 -5.5 309,421 + Pf1N1B4_5544 0.20 -0.6 309,422 - -1.0 309,565 + Pf1N1B4_5544 0.27 +0.0 309,565 + Pf1N1B4_5544 0.27 +0.0 309,569 + Pf1N1B4_5544 0.27 +0.0 309,570 - Pf1N1B4_5544 0.28 +0.0 309,630 + Pf1N1B4_5544 0.31 +0.0 309,631 - Pf1N1B4_5544 0.31 -2.0 309,824 + Pf1N1B4_5544 0.40 -1.2 309,907 + Pf1N1B4_5544 0.44 -1.5 309,907 + Pf1N1B4_5544 0.44 -1.2 309,907 + -1.8 309,908 - Pf1N1B4_5544 0.44 -1.7 309,908 - -2.3 309,911 + Pf1N1B4_5544 0.45 -0.5 309,911 + Pf1N1B4_5544 0.45 +0.0 309,911 + Pf1N1B4_5544 0.45 -4.4 309,912 - Pf1N1B4_5544 0.45 -5.6 309,912 - Pf1N1B4_5544 0.45 -3.2 309,985 + Pf1N1B4_5544 0.48 -0.7 309,985 + +0.0 309,986 - Pf1N1B4_5544 0.48 +0.0 310,018 + Pf1N1B4_5544 0.50 +0.0 310,019 - Pf1N1B4_5544 0.50 +0.0 310,040 + Pf1N1B4_5544 0.51 -2.0 310,163 + Pf1N1B4_5544 0.57 -2.0 310,164 - Pf1N1B4_5544 0.57 -2.7 310,181 + Pf1N1B4_5544 0.58 -1.5 310,182 - Pf1N1B4_5544 0.58 -1.9 310,182 - Pf1N1B4_5544 0.58 +0.0 310,341 - Pf1N1B4_5544 0.66 -2.6 310,341 - Pf1N1B4_5544 0.66 +0.0 310,372 + Pf1N1B4_5544 0.68 +0.0 310,373 - Pf1N1B4_5544 0.68 +0.0 310,373 - Pf1N1B4_5544 0.68 -0.9 310,373 - Pf1N1B4_5544 0.68 +0.0 310,373 - Pf1N1B4_5544 0.68 -1.4 310,375 + -6.1 310,375 + Pf1N1B4_5544 0.68 -2.8 310,376 - +0.0 310,418 + Pf1N1B4_5544 0.70 -1.4 310,554 + Pf1N1B4_5544 0.77 +0.0 310,555 - Pf1N1B4_5544 0.77 -1.6 310,643 + Pf1N1B4_5544 0.81 -4.7 310,643 + Pf1N1B4_5544 0.81 -2.1 310,643 + Pf1N1B4_5544 0.81 -5.6 310,644 - Pf1N1B4_5544 0.81 -1.1 310,751 + Pf1N1B4_5544 0.87 +0.0 310,752 - Pf1N1B4_5544 0.87 +0.0 310,810 + Pf1N1B4_5544 0.90 +0.0 310,811 - Pf1N1B4_5544 0.90 +0.0 310,823 - +0.0 310,902 + +0.0 310,946 + +1.0 310,947 - +0.0 311,014 - +0.0 311,014 - +0.0 311,041 + +0.0 311,116 + +0.0 311,116 + +0.0 311,142 + +0.0 311,158 + +0.0 311,191 + +0.0 311,191 + +0.0 311,191 + +0.0 311,191 + -1.6 311,192 - -4.2 311,192 - +0.0 311,192 - +0.0 311,230 - -3.5 311,261 + +0.0 311,262 - +0.0 311,262 - -1.1 311,354 + -3.0 311,354 + +0.0 311,354 + +0.0 311,354 + +0.0 311,355 - +0.0 311,524 + -1.0 311,524 + -3.3 311,525 - -1.9 311,525 - +0.0 311,545 + +0.0 311,545 + -1.5 311,545 + +0.0 311,546 - -3.6 311,607 + +0.0 311,607 + -1.3 311,607 + +0.0 311,607 + +0.0 311,607 + +0.0 311,608 - +0.0 311,608 - -1.1 311,608 - -1.7 311,630 + -3.7 311,630 + -3.5 311,630 + +0.0 311,631 - +0.0 311,631 - +0.0 311,631 - +0.0 311,631 - -4.2 311,631 - -1.1 311,634 - -0.2 311,742 + +0.0 311,743 - +0.0 311,743 - +0.0 311,746 + +0.0 311,746 + +0.0 311,747 - +0.0 311,843 + +0.0 311,844 - +0.0 311,881 - -1.3 312,015 - +0.0
Or see this region's nucleotide sequence