Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5460 and Pf1N1B4_5461 are separated by 307 nucleotides Pf1N1B4_5461 and Pf1N1B4_5462 are separated by 559 nucleotides
Pf1N1B4_5460 - TolA protein, at 207,111 to 207,977
_5460
Pf1N1B4_5461 - hypothetical protein, at 208,285 to 208,695
_5461
Pf1N1B4_5462 - hypothetical protein, at 209,255 to 210,247
_5462
Position (kb) 208
209 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 207.515 kb on + strand, within Pf1N1B4_5460 at 207.516 kb on - strand, within Pf1N1B4_5460 at 207.935 kb on + strand at 208.050 kb on + strand at 208.051 kb on - strand at 208.051 kb on - strand at 208.051 kb on - strand at 208.051 kb on - strand at 208.070 kb on + strand at 208.071 kb on - strand at 208.071 kb on - strand at 208.071 kb on - strand at 208.071 kb on - strand at 208.071 kb on - strand at 208.273 kb on - strand at 208.276 kb on + strand at 208.276 kb on + strand at 208.324 kb on + strand at 208.324 kb on + strand at 208.325 kb on - strand at 208.325 kb on - strand at 208.377 kb on - strand, within Pf1N1B4_5461 at 208.377 kb on - strand, within Pf1N1B4_5461 at 208.377 kb on - strand, within Pf1N1B4_5461 at 208.377 kb on - strand, within Pf1N1B4_5461 at 208.411 kb on + strand, within Pf1N1B4_5461 at 208.412 kb on - strand, within Pf1N1B4_5461 at 208.526 kb on - strand, within Pf1N1B4_5461 at 208.553 kb on - strand, within Pf1N1B4_5461 at 208.553 kb on - strand, within Pf1N1B4_5461 at 208.557 kb on - strand, within Pf1N1B4_5461 at 208.557 kb on - strand, within Pf1N1B4_5461 at 208.618 kb on - strand at 208.667 kb on + strand at 208.668 kb on - strand at 208.726 kb on + strand at 208.727 kb on - strand at 208.727 kb on - strand at 208.727 kb on - strand at 208.727 kb on - strand at 208.918 kb on + strand at 208.918 kb on + strand at 208.933 kb on - strand at 208.933 kb on - strand at 208.972 kb on - strand at 208.972 kb on - strand at 208.972 kb on - strand at 208.975 kb on + strand at 208.976 kb on - strand at 208.976 kb on - strand at 208.976 kb on - strand at 209.015 kb on - strand at 209.015 kb on - strand at 209.026 kb on - strand at 209.026 kb on - strand at 209.026 kb on - strand at 209.026 kb on - strand at 209.026 kb on - strand at 209.141 kb on - strand at 209.228 kb on - strand at 209.231 kb on - strand at 209.231 kb on - strand at 209.237 kb on - strand at 209.287 kb on - strand at 209.318 kb on - strand at 209.318 kb on - strand at 209.351 kb on - strand at 209.351 kb on - strand at 209.351 kb on - strand at 209.369 kb on - strand, within Pf1N1B4_5462 at 209.386 kb on + strand at 209.387 kb on - strand, within Pf1N1B4_5462 at 209.387 kb on - strand at 209.387 kb on - strand at 209.511 kb on - strand, within Pf1N1B4_5462 at 209.511 kb on - strand, within Pf1N1B4_5462 at 209.511 kb on - strand, within Pf1N1B4_5462 at 209.511 kb on - strand, within Pf1N1B4_5462 at 209.554 kb on + strand, within Pf1N1B4_5462 at 209.554 kb on + strand, within Pf1N1B4_5462 at 209.587 kb on - strand, within Pf1N1B4_5462 at 209.587 kb on - strand, within Pf1N1B4_5462 at 209.691 kb on + strand, within Pf1N1B4_5462
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 207,515 + Pf1N1B4_5460 0.47 +0.0 207,516 - Pf1N1B4_5460 0.47 -1.4 207,935 + +1.5 208,050 + +0.0 208,051 - +0.0 208,051 - -1.1 208,051 - +0.0 208,051 - +0.0 208,070 + -0.9 208,071 - +0.0 208,071 - -1.5 208,071 - +0.0 208,071 - -4.6 208,071 - -3.1 208,273 - +0.0 208,276 + +0.0 208,276 + -2.0 208,324 + +0.0 208,324 + -1.7 208,325 - +0.0 208,325 - +0.0 208,377 - Pf1N1B4_5461 0.22 +0.0 208,377 - Pf1N1B4_5461 0.22 +0.0 208,377 - Pf1N1B4_5461 0.22 +0.0 208,377 - Pf1N1B4_5461 0.22 -3.1 208,411 + Pf1N1B4_5461 0.31 +0.0 208,412 - Pf1N1B4_5461 0.31 -3.7 208,526 - Pf1N1B4_5461 0.59 +0.0 208,553 - Pf1N1B4_5461 0.65 +0.0 208,553 - Pf1N1B4_5461 0.65 -4.3 208,557 - Pf1N1B4_5461 0.66 +0.0 208,557 - Pf1N1B4_5461 0.66 -5.5 208,618 - +0.0 208,667 + +0.0 208,668 - +0.0 208,726 + -3.1 208,727 - +0.0 208,727 - +0.0 208,727 - -0.5 208,727 - +0.0 208,918 + -1.2 208,918 + +0.0 208,933 - -1.5 208,933 - +0.0 208,972 - -2.0 208,972 - +0.0 208,972 - -3.3 208,975 + +0.0 208,976 - -1.6 208,976 - +0.0 208,976 - +0.0 209,015 - +0.0 209,015 - +0.0 209,026 - -1.5 209,026 - +0.0 209,026 - +0.0 209,026 - +0.0 209,026 - -1.2 209,141 - -0.7 209,228 - +0.0 209,231 - -1.4 209,231 - +0.0 209,237 - -2.2 209,287 - -1.9 209,318 - +0.0 209,318 - -1.6 209,351 - +0.0 209,351 - +0.0 209,351 - +0.0 209,369 - Pf1N1B4_5462 0.11 +0.0 209,386 + -0.2 209,387 - Pf1N1B4_5462 0.13 -1.5 209,387 - -5.6 209,387 - +0.0 209,511 - Pf1N1B4_5462 0.26 +0.0 209,511 - Pf1N1B4_5462 0.26 +0.0 209,511 - Pf1N1B4_5462 0.26 +0.0 209,511 - Pf1N1B4_5462 0.26 -1.0 209,554 + Pf1N1B4_5462 0.30 +0.0 209,554 + Pf1N1B4_5462 0.30 +0.0 209,587 - Pf1N1B4_5462 0.33 +0.0 209,587 - Pf1N1B4_5462 0.33 -1.8 209,691 + Pf1N1B4_5462 0.44 +0.0
Or see this region's nucleotide sequence