Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5106 and Pf1N1B4_5107 are separated by 231 nucleotides Pf1N1B4_5107 and Pf1N1B4_5238 are separated by 202 nucleotides Pf1N1B4_5238 and Pf1N1B4_5108 are separated by 152 nucleotides
Pf1N1B4_5106 - Dipeptide-binding ABC transporter, periplasmic substrate-binding component (TC 3.A.1.5.2); Putative hemin-binding lipoprotein, at 5,659,544 to 5,661,058
_5106
Pf1N1B4_5107 - Putative collagenase, at 5,661,290 to 5,663,290
_5107
Pf1N1B4_5238 - tRNA-Gln-TTG, at 5,663,493 to 5,663,564
_5238
Pf1N1B4_5108 - Capsule biosynthesis protein capA, at 5,663,717 to 5,664,829
_5108
Position (kb)
5661
5662
5663
5664 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 5660.397 kb on + strand, within Pf1N1B4_5106 at 5660.474 kb on + strand, within Pf1N1B4_5106 at 5660.480 kb on + strand at 5660.481 kb on - strand, within Pf1N1B4_5106 at 5660.481 kb on - strand, within Pf1N1B4_5106 at 5660.594 kb on + strand at 5660.733 kb on - strand at 5660.751 kb on - strand, within Pf1N1B4_5106 at 5660.822 kb on + strand, within Pf1N1B4_5106 at 5660.822 kb on + strand, within Pf1N1B4_5106 at 5660.822 kb on + strand, within Pf1N1B4_5106 at 5660.823 kb on - strand, within Pf1N1B4_5106 at 5660.823 kb on - strand at 5660.823 kb on - strand, within Pf1N1B4_5106 at 5660.823 kb on - strand, within Pf1N1B4_5106 at 5660.835 kb on - strand, within Pf1N1B4_5106 at 5660.909 kb on + strand at 5660.909 kb on + strand at 5660.910 kb on - strand at 5660.910 kb on - strand at 5660.972 kb on + strand at 5660.973 kb on - strand at 5660.973 kb on - strand at 5660.973 kb on - strand at 5661.384 kb on - strand at 5661.820 kb on + strand, within Pf1N1B4_5107 at 5662.719 kb on - strand, within Pf1N1B4_5107 at 5662.940 kb on + strand, within Pf1N1B4_5107 at 5662.941 kb on - strand, within Pf1N1B4_5107 at 5663.467 kb on + strand at 5663.631 kb on + strand at 5663.820 kb on - strand at 5663.828 kb on + strand at 5663.828 kb on + strand at 5663.829 kb on - strand, within Pf1N1B4_5108 at 5663.829 kb on - strand, within Pf1N1B4_5108 at 5663.906 kb on + strand, within Pf1N1B4_5108 at 5663.907 kb on - strand, within Pf1N1B4_5108 at 5664.137 kb on - strand, within Pf1N1B4_5108
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 5,660,397 + Pf1N1B4_5106 0.56 -0.7 5,660,474 + Pf1N1B4_5106 0.61 -4.3 5,660,480 + -0.7 5,660,481 - Pf1N1B4_5106 0.62 -0.2 5,660,481 - Pf1N1B4_5106 0.62 -0.2 5,660,594 + -2.0 5,660,733 - -0.2 5,660,751 - Pf1N1B4_5106 0.80 -1.7 5,660,822 + Pf1N1B4_5106 0.84 -0.2 5,660,822 + Pf1N1B4_5106 0.84 -2.4 5,660,822 + Pf1N1B4_5106 0.84 -0.2 5,660,823 - Pf1N1B4_5106 0.84 +1.0 5,660,823 - -3.6 5,660,823 - Pf1N1B4_5106 0.84 -2.1 5,660,823 - Pf1N1B4_5106 0.84 -0.2 5,660,835 - Pf1N1B4_5106 0.85 -0.2 5,660,909 + -0.9 5,660,909 + -0.2 5,660,910 - -5.0 5,660,910 - -0.2 5,660,972 + -0.4 5,660,973 - -0.2 5,660,973 - -2.0 5,660,973 - -0.2 5,661,384 - -3.4 5,661,820 + Pf1N1B4_5107 0.26 -4.4 5,662,719 - Pf1N1B4_5107 0.71 -3.5 5,662,940 + Pf1N1B4_5107 0.82 -2.8 5,662,941 - Pf1N1B4_5107 0.83 -0.2 5,663,467 + -0.2 5,663,631 + -0.2 5,663,820 - -4.2 5,663,828 + -0.2 5,663,828 + -0.2 5,663,829 - Pf1N1B4_5108 0.10 -1.3 5,663,829 - Pf1N1B4_5108 0.10 -0.2 5,663,906 + Pf1N1B4_5108 0.17 -0.2 5,663,907 - Pf1N1B4_5108 0.17 -2.3 5,664,137 - Pf1N1B4_5108 0.38 -1.4
Or see this region's nucleotide sequence