Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4899 and Pf1N1B4_4900 are separated by 97 nucleotides Pf1N1B4_4900 and Pf1N1B4_4901 are separated by 211 nucleotides
Pf1N1B4_4899 - Secreted alkaline metalloproteinase (EC 3.4.24.-), PrtA/B/C/G homolog, at 5,421,215 to 5,422,672
_4899
Pf1N1B4_4900 - Alkaline proteinase inhibitor precursor, at 5,422,770 to 5,423,192
_4900
Pf1N1B4_4901 - ABC-type protease exporter, ATP-binding component PrtD/AprD, at 5,423,404 to 5,425,179
_4901
Position (kb)
5422
5423
5424 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 5421.771 kb on - strand, within Pf1N1B4_4899 at 5421.875 kb on + strand, within Pf1N1B4_4899 at 5421.875 kb on + strand, within Pf1N1B4_4899 at 5421.897 kb on - strand, within Pf1N1B4_4899 at 5421.912 kb on - strand at 5422.014 kb on - strand, within Pf1N1B4_4899 at 5422.052 kb on + strand, within Pf1N1B4_4899 at 5422.052 kb on + strand, within Pf1N1B4_4899 at 5422.735 kb on - strand at 5422.741 kb on + strand at 5422.742 kb on - strand at 5422.747 kb on + strand at 5422.760 kb on - strand at 5422.787 kb on + strand at 5422.788 kb on - strand at 5422.791 kb on + strand at 5422.792 kb on - strand at 5422.792 kb on - strand at 5422.843 kb on + strand, within Pf1N1B4_4900 at 5423.353 kb on + strand at 5423.384 kb on + strand at 5423.385 kb on - strand at 5423.385 kb on - strand at 5423.387 kb on + strand at 5423.460 kb on + strand at 5423.461 kb on - strand at 5423.531 kb on - strand at 5423.532 kb on + strand at 5423.843 kb on - strand, within Pf1N1B4_4901
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 5,421,771 - Pf1N1B4_4899 0.38 -2.0 5,421,875 + Pf1N1B4_4899 0.45 -0.2 5,421,875 + Pf1N1B4_4899 0.45 -0.2 5,421,897 - Pf1N1B4_4899 0.47 -0.2 5,421,912 - -0.2 5,422,014 - Pf1N1B4_4899 0.55 -0.2 5,422,052 + Pf1N1B4_4899 0.57 -0.2 5,422,052 + Pf1N1B4_4899 0.57 -0.2 5,422,735 - -0.2 5,422,741 + -0.2 5,422,742 - -1.3 5,422,747 + -1.4 5,422,760 - -0.2 5,422,787 + -0.2 5,422,788 - -0.2 5,422,791 + -3.9 5,422,792 - -0.2 5,422,792 - -0.2 5,422,843 + Pf1N1B4_4900 0.17 -3.0 5,423,353 + -4.6 5,423,384 + -1.6 5,423,385 - -0.2 5,423,385 - -1.2 5,423,387 + -3.0 5,423,460 + -0.2 5,423,461 - -3.0 5,423,531 - -2.6 5,423,532 + -3.2 5,423,843 - Pf1N1B4_4901 0.25 -1.9
Or see this region's nucleotide sequence