Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4733 and Pf1N1B4_4734 are separated by 32 nucleotides Pf1N1B4_4734 and Pf1N1B4_4735 are separated by 62 nucleotides Pf1N1B4_4735 and Pf1N1B4_4736 are separated by 51 nucleotides Pf1N1B4_4736 and Pf1N1B4_4737 are separated by 1 nucleotides
Pf1N1B4_4733 - Fap system putative outer membrane protein, at 5,231,152 to 5,231,889
_4733
Pf1N1B4_4734 - Fap protein with C39 domain, at 5,231,922 to 5,232,602
_4734
Pf1N1B4_4735 - Fap amyloid fibril major component, at 5,232,665 to 5,233,954
_4735
Pf1N1B4_4736 - Fap amyloid fibril minor component, at 5,234,006 to 5,234,578
_4736
Pf1N1B4_4737 - hypothetical protein, at 5,234,580 to 5,234,801
_4737
Position (kb)
5232
5233
5234 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 5231.698 kb on + strand, within Pf1N1B4_4733 at 5231.698 kb on + strand, within Pf1N1B4_4733 at 5232.145 kb on + strand at 5232.145 kb on + strand, within Pf1N1B4_4734 at 5232.146 kb on - strand, within Pf1N1B4_4734 at 5232.213 kb on + strand, within Pf1N1B4_4734 at 5232.214 kb on - strand, within Pf1N1B4_4734 at 5232.397 kb on + strand, within Pf1N1B4_4734 at 5232.411 kb on + strand at 5232.476 kb on + strand, within Pf1N1B4_4734 at 5232.487 kb on - strand, within Pf1N1B4_4734 at 5232.505 kb on + strand, within Pf1N1B4_4734 at 5232.505 kb on + strand, within Pf1N1B4_4734 at 5232.572 kb on - strand at 5232.572 kb on - strand at 5232.831 kb on + strand, within Pf1N1B4_4735 at 5232.831 kb on + strand at 5232.831 kb on + strand, within Pf1N1B4_4735 at 5232.832 kb on - strand, within Pf1N1B4_4735 at 5232.832 kb on - strand, within Pf1N1B4_4735 at 5232.832 kb on - strand, within Pf1N1B4_4735 at 5232.867 kb on + strand at 5232.868 kb on - strand, within Pf1N1B4_4735 at 5232.904 kb on + strand, within Pf1N1B4_4735 at 5232.953 kb on - strand, within Pf1N1B4_4735 at 5233.035 kb on - strand, within Pf1N1B4_4735 at 5233.035 kb on - strand, within Pf1N1B4_4735 at 5233.349 kb on - strand, within Pf1N1B4_4735 at 5233.815 kb on + strand, within Pf1N1B4_4735 at 5233.847 kb on - strand at 5233.889 kb on + strand at 5233.890 kb on - strand at 5233.968 kb on - strand at 5233.971 kb on + strand at 5233.971 kb on + strand at 5233.971 kb on + strand at 5233.982 kb on - strand at 5233.994 kb on + strand at 5234.021 kb on + strand at 5234.218 kb on + strand, within Pf1N1B4_4736 at 5234.219 kb on - strand, within Pf1N1B4_4736 at 5234.224 kb on + strand, within Pf1N1B4_4736 at 5234.224 kb on + strand, within Pf1N1B4_4736 at 5234.225 kb on - strand, within Pf1N1B4_4736 at 5234.228 kb on + strand, within Pf1N1B4_4736 at 5234.735 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 5,231,698 + Pf1N1B4_4733 0.74 -4.9 5,231,698 + Pf1N1B4_4733 0.74 -1.1 5,232,145 + -0.2 5,232,145 + Pf1N1B4_4734 0.33 -0.2 5,232,146 - Pf1N1B4_4734 0.33 -2.8 5,232,213 + Pf1N1B4_4734 0.43 -0.2 5,232,214 - Pf1N1B4_4734 0.43 -0.2 5,232,397 + Pf1N1B4_4734 0.70 -0.5 5,232,411 + -0.2 5,232,476 + Pf1N1B4_4734 0.81 -0.2 5,232,487 - Pf1N1B4_4734 0.83 -2.0 5,232,505 + Pf1N1B4_4734 0.86 -0.2 5,232,505 + Pf1N1B4_4734 0.86 -1.5 5,232,572 - -4.0 5,232,572 - -2.2 5,232,831 + Pf1N1B4_4735 0.13 -2.9 5,232,831 + -0.2 5,232,831 + Pf1N1B4_4735 0.13 -0.2 5,232,832 - Pf1N1B4_4735 0.13 -0.2 5,232,832 - Pf1N1B4_4735 0.13 -0.2 5,232,832 - Pf1N1B4_4735 0.13 -1.8 5,232,867 + -0.8 5,232,868 - Pf1N1B4_4735 0.16 -0.2 5,232,904 + Pf1N1B4_4735 0.19 -1.7 5,232,953 - Pf1N1B4_4735 0.22 -0.2 5,233,035 - Pf1N1B4_4735 0.29 -0.2 5,233,035 - Pf1N1B4_4735 0.29 -2.8 5,233,349 - Pf1N1B4_4735 0.53 -0.2 5,233,815 + Pf1N1B4_4735 0.89 -0.2 5,233,847 - -0.3 5,233,889 + -0.2 5,233,890 - -4.8 5,233,968 - -0.9 5,233,971 + -0.2 5,233,971 + -0.2 5,233,971 + -1.3 5,233,982 - -0.2 5,233,994 + -0.2 5,234,021 + -0.2 5,234,218 + Pf1N1B4_4736 0.37 -2.2 5,234,219 - Pf1N1B4_4736 0.37 -2.1 5,234,224 + Pf1N1B4_4736 0.38 -1.0 5,234,224 + Pf1N1B4_4736 0.38 -0.2 5,234,225 - Pf1N1B4_4736 0.38 -0.2 5,234,228 + Pf1N1B4_4736 0.39 -1.1 5,234,735 + -2.1
Or see this region's nucleotide sequence