Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4715

Experiment: Nitrate reduction

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4713 and Pf1N1B4_4714 overlap by 47 nucleotidesPf1N1B4_4714 and Pf1N1B4_4715 are separated by 175 nucleotidesPf1N1B4_4715 and Pf1N1B4_4716 are separated by 101 nucleotides Pf1N1B4_4713 - Xanthine permease, at 5,210,008 to 5,211,483 _4713 Pf1N1B4_4714 - hypothetical protein, at 5,211,437 to 5,211,553 _4714 Pf1N1B4_4715 - Transcriptional regulator, at 5,211,729 to 5,212,622 _4715 Pf1N1B4_4716 - Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2), at 5,212,724 to 5,213,815 _4716 Position (kb) 5211 5212 5213Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 5211.299 kb on + strand, within Pf1N1B4_4713at 5211.376 kb on + strandat 5211.376 kb on + strandat 5211.377 kb on - strandat 5211.377 kb on - strandat 5211.562 kb on + strandat 5211.563 kb on - strandat 5211.563 kb on - strandat 5211.563 kb on - strandat 5211.727 kb on - strandat 5211.727 kb on - strandat 5211.727 kb on - strandat 5212.091 kb on - strand, within Pf1N1B4_4715at 5212.154 kb on - strand, within Pf1N1B4_4715at 5212.154 kb on - strand, within Pf1N1B4_4715at 5212.652 kb on - strandat 5212.811 kb on + strandat 5212.812 kb on - strandat 5212.883 kb on + strandat 5213.105 kb on + strand, within Pf1N1B4_4716at 5213.105 kb on + strand, within Pf1N1B4_4716at 5213.207 kb on + strand, within Pf1N1B4_4716at 5213.282 kb on + strand, within Pf1N1B4_4716at 5213.283 kb on - strand, within Pf1N1B4_4716at 5213.352 kb on + strand, within Pf1N1B4_4716at 5213.432 kb on + strand, within Pf1N1B4_4716

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction
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5,211,299 + Pf1N1B4_4713 0.87 -0.2
5,211,376 + -0.2
5,211,376 + -0.2
5,211,377 - -0.2
5,211,377 - +0.2
5,211,562 + -1.6
5,211,563 - -5.9
5,211,563 - -0.2
5,211,563 - -0.2
5,211,727 - -0.5
5,211,727 - -0.2
5,211,727 - -0.2
5,212,091 - Pf1N1B4_4715 0.40 -0.2
5,212,154 - Pf1N1B4_4715 0.48 -0.2
5,212,154 - Pf1N1B4_4715 0.48 -3.1
5,212,652 - -0.2
5,212,811 + -0.2
5,212,812 - -0.2
5,212,883 + -0.2
5,213,105 + Pf1N1B4_4716 0.35 -1.7
5,213,105 + Pf1N1B4_4716 0.35 -5.4
5,213,207 + Pf1N1B4_4716 0.44 -0.2
5,213,282 + Pf1N1B4_4716 0.51 -0.2
5,213,283 - Pf1N1B4_4716 0.51 -2.2
5,213,352 + Pf1N1B4_4716 0.58 -1.0
5,213,432 + Pf1N1B4_4716 0.65 -0.2

Or see this region's nucleotide sequence