Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4580 and Pf1N1B4_4581 are separated by 4 nucleotides Pf1N1B4_4581 and Pf1N1B4_4582 are separated by 176 nucleotides Pf1N1B4_4582 and Pf1N1B4_4583 are separated by 142 nucleotides Pf1N1B4_4583 and Pf1N1B4_4584 are separated by 24 nucleotides
Pf1N1B4_4580 - Fe2+-dicitrate sensor, membrane component, at 5,051,945 to 5,052,931
_4580
Pf1N1B4_4581 - RNA polymerase sigma-70 factor, ECF subfamily, at 5,052,936 to 5,053,460
_4581
Pf1N1B4_4582 - hypothetical protein, at 5,053,637 to 5,053,819
_4582
Pf1N1B4_4583 - Isoquinoline 1-oxidoreductase alpha subunit (EC 1.3.99.16), at 5,053,962 to 5,054,417
_4583
Pf1N1B4_4584 - Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16), at 5,054,442 to 5,056,610
_4584
Position (kb)
5053
5054 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 5052.714 kb on - strand, within Pf1N1B4_4580 at 5052.774 kb on + strand, within Pf1N1B4_4580 at 5052.845 kb on + strand at 5052.871 kb on - strand at 5053.003 kb on + strand, within Pf1N1B4_4581 at 5053.082 kb on + strand at 5053.327 kb on - strand at 5053.421 kb on + strand at 5053.421 kb on + strand at 5053.422 kb on - strand at 5053.423 kb on + strand at 5053.598 kb on + strand at 5053.598 kb on + strand at 5053.650 kb on - strand at 5053.660 kb on + strand, within Pf1N1B4_4582 at 5053.660 kb on + strand, within Pf1N1B4_4582 at 5053.660 kb on + strand, within Pf1N1B4_4582 at 5053.774 kb on - strand, within Pf1N1B4_4582 at 5053.973 kb on + strand at 5053.973 kb on + strand at 5053.977 kb on - strand at 5054.104 kb on + strand, within Pf1N1B4_4583 at 5054.105 kb on - strand, within Pf1N1B4_4583
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 5,052,714 - Pf1N1B4_4580 0.78 -1.6 5,052,774 + Pf1N1B4_4580 0.84 -0.2 5,052,845 + -0.2 5,052,871 - -0.2 5,053,003 + Pf1N1B4_4581 0.13 -0.2 5,053,082 + -0.2 5,053,327 - -0.2 5,053,421 + -0.2 5,053,421 + -2.1 5,053,422 - -0.2 5,053,423 + -0.2 5,053,598 + -0.2 5,053,598 + -3.0 5,053,650 - -0.2 5,053,660 + Pf1N1B4_4582 0.13 -0.2 5,053,660 + Pf1N1B4_4582 0.13 -0.2 5,053,660 + Pf1N1B4_4582 0.13 -4.0 5,053,774 - Pf1N1B4_4582 0.75 -0.2 5,053,973 + -0.2 5,053,973 + -0.2 5,053,977 - -3.0 5,054,104 + Pf1N1B4_4583 0.31 -0.2 5,054,105 - Pf1N1B4_4583 0.31 -0.2
Or see this region's nucleotide sequence