Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4582

Experiment: Nitrate reduction

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4580 and Pf1N1B4_4581 are separated by 4 nucleotidesPf1N1B4_4581 and Pf1N1B4_4582 are separated by 176 nucleotidesPf1N1B4_4582 and Pf1N1B4_4583 are separated by 142 nucleotidesPf1N1B4_4583 and Pf1N1B4_4584 are separated by 24 nucleotides Pf1N1B4_4580 - Fe2+-dicitrate sensor, membrane component, at 5,051,945 to 5,052,931 _4580 Pf1N1B4_4581 - RNA polymerase sigma-70 factor, ECF subfamily, at 5,052,936 to 5,053,460 _4581 Pf1N1B4_4582 - hypothetical protein, at 5,053,637 to 5,053,819 _4582 Pf1N1B4_4583 - Isoquinoline 1-oxidoreductase alpha subunit (EC 1.3.99.16), at 5,053,962 to 5,054,417 _4583 Pf1N1B4_4584 - Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16), at 5,054,442 to 5,056,610 _4584 Position (kb) 5053 5054Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 5052.714 kb on - strand, within Pf1N1B4_4580at 5052.774 kb on + strand, within Pf1N1B4_4580at 5052.845 kb on + strandat 5052.871 kb on - strandat 5053.003 kb on + strand, within Pf1N1B4_4581at 5053.082 kb on + strandat 5053.327 kb on - strandat 5053.421 kb on + strandat 5053.421 kb on + strandat 5053.422 kb on - strandat 5053.423 kb on + strandat 5053.598 kb on + strandat 5053.598 kb on + strandat 5053.650 kb on - strandat 5053.660 kb on + strand, within Pf1N1B4_4582at 5053.660 kb on + strand, within Pf1N1B4_4582at 5053.660 kb on + strand, within Pf1N1B4_4582at 5053.774 kb on - strand, within Pf1N1B4_4582at 5053.973 kb on + strandat 5053.973 kb on + strandat 5053.977 kb on - strandat 5054.104 kb on + strand, within Pf1N1B4_4583at 5054.105 kb on - strand, within Pf1N1B4_4583

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction
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5,052,714 - Pf1N1B4_4580 0.78 -1.6
5,052,774 + Pf1N1B4_4580 0.84 -0.2
5,052,845 + -0.2
5,052,871 - -0.2
5,053,003 + Pf1N1B4_4581 0.13 -0.2
5,053,082 + -0.2
5,053,327 - -0.2
5,053,421 + -0.2
5,053,421 + -2.1
5,053,422 - -0.2
5,053,423 + -0.2
5,053,598 + -0.2
5,053,598 + -3.0
5,053,650 - -0.2
5,053,660 + Pf1N1B4_4582 0.13 -0.2
5,053,660 + Pf1N1B4_4582 0.13 -0.2
5,053,660 + Pf1N1B4_4582 0.13 -4.0
5,053,774 - Pf1N1B4_4582 0.75 -0.2
5,053,973 + -0.2
5,053,973 + -0.2
5,053,977 - -3.0
5,054,104 + Pf1N1B4_4583 0.31 -0.2
5,054,105 - Pf1N1B4_4583 0.31 -0.2

Or see this region's nucleotide sequence