Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4570 and Pf1N1B4_4571 are separated by 151 nucleotides Pf1N1B4_4571 and Pf1N1B4_4572 are separated by 123 nucleotides Pf1N1B4_4572 and Pf1N1B4_4573 overlap by 8 nucleotides
Pf1N1B4_4570 - Probable carboxyvinyl-carboxyphosphonate phosphorylmutase (EC 2.7.8.23), at 5,042,654 to 5,043,505
_4570
Pf1N1B4_4571 - ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63), at 5,043,657 to 5,044,589
_4571
Pf1N1B4_4572 - hypothetical protein, at 5,044,713 to 5,045,039
_4572
Pf1N1B4_4573 - transporter, LysE family, at 5,045,032 to 5,045,739
_4573
Position (kb)
5043
5044
5045 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 5042.736 kb on + strand at 5042.952 kb on + strand, within Pf1N1B4_4570 at 5042.953 kb on - strand, within Pf1N1B4_4570 at 5042.953 kb on - strand, within Pf1N1B4_4570 at 5043.004 kb on - strand, within Pf1N1B4_4570 at 5043.066 kb on + strand, within Pf1N1B4_4570 at 5043.067 kb on - strand, within Pf1N1B4_4570 at 5043.067 kb on - strand, within Pf1N1B4_4570 at 5043.338 kb on - strand, within Pf1N1B4_4570 at 5043.760 kb on + strand, within Pf1N1B4_4571 at 5043.760 kb on + strand, within Pf1N1B4_4571 at 5043.760 kb on + strand, within Pf1N1B4_4571 at 5043.765 kb on - strand, within Pf1N1B4_4571 at 5043.765 kb on - strand, within Pf1N1B4_4571 at 5043.875 kb on - strand, within Pf1N1B4_4571 at 5044.639 kb on + strand at 5044.639 kb on + strand at 5044.639 kb on + strand at 5044.640 kb on - strand at 5044.640 kb on - strand at 5044.640 kb on - strand at 5044.640 kb on - strand at 5044.640 kb on - strand at 5044.739 kb on + strand at 5044.740 kb on - strand at 5044.820 kb on + strand, within Pf1N1B4_4572 at 5045.112 kb on + strand at 5045.113 kb on - strand, within Pf1N1B4_4573 at 5045.113 kb on - strand, within Pf1N1B4_4573 at 5045.113 kb on - strand at 5045.292 kb on - strand, within Pf1N1B4_4573 at 5045.292 kb on - strand, within Pf1N1B4_4573 at 5045.537 kb on + strand, within Pf1N1B4_4573 at 5045.537 kb on + strand, within Pf1N1B4_4573 at 5045.538 kb on - strand, within Pf1N1B4_4573 at 5045.538 kb on - strand, within Pf1N1B4_4573 at 5045.539 kb on + strand, within Pf1N1B4_4573 at 5045.540 kb on - strand, within Pf1N1B4_4573 at 5045.574 kb on - strand, within Pf1N1B4_4573 at 5045.574 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 5,042,736 + -0.1 5,042,952 + Pf1N1B4_4570 0.35 -1.4 5,042,953 - Pf1N1B4_4570 0.35 -0.1 5,042,953 - Pf1N1B4_4570 0.35 -0.1 5,043,004 - Pf1N1B4_4570 0.41 -3.4 5,043,066 + Pf1N1B4_4570 0.48 -0.7 5,043,067 - Pf1N1B4_4570 0.48 -0.1 5,043,067 - Pf1N1B4_4570 0.48 -0.1 5,043,338 - Pf1N1B4_4570 0.80 +0.7 5,043,760 + Pf1N1B4_4571 0.11 -0.1 5,043,760 + Pf1N1B4_4571 0.11 -0.1 5,043,760 + Pf1N1B4_4571 0.11 -4.7 5,043,765 - Pf1N1B4_4571 0.12 -0.1 5,043,765 - Pf1N1B4_4571 0.12 -0.1 5,043,875 - Pf1N1B4_4571 0.23 -0.1 5,044,639 + -0.1 5,044,639 + -1.1 5,044,639 + -0.1 5,044,640 - -1.9 5,044,640 - -3.5 5,044,640 - -0.1 5,044,640 - -4.2 5,044,640 - -0.1 5,044,739 + -2.5 5,044,740 - -0.1 5,044,820 + Pf1N1B4_4572 0.33 -0.1 5,045,112 + -2.8 5,045,113 - Pf1N1B4_4573 0.11 -0.1 5,045,113 - Pf1N1B4_4573 0.11 -0.1 5,045,113 - -0.1 5,045,292 - Pf1N1B4_4573 0.37 -0.1 5,045,292 - Pf1N1B4_4573 0.37 -0.1 5,045,537 + Pf1N1B4_4573 0.71 -0.2 5,045,537 + Pf1N1B4_4573 0.71 +1.4 5,045,538 - Pf1N1B4_4573 0.71 -0.5 5,045,538 - Pf1N1B4_4573 0.71 -1.4 5,045,539 + Pf1N1B4_4573 0.72 -2.9 5,045,540 - Pf1N1B4_4573 0.72 -0.1 5,045,574 - Pf1N1B4_4573 0.77 -0.1 5,045,574 - -0.1
Or see this region's nucleotide sequence