Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4571

Experiment: Nitrate reduction

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4570 and Pf1N1B4_4571 are separated by 151 nucleotidesPf1N1B4_4571 and Pf1N1B4_4572 are separated by 123 nucleotidesPf1N1B4_4572 and Pf1N1B4_4573 overlap by 8 nucleotides Pf1N1B4_4570 - Probable carboxyvinyl-carboxyphosphonate phosphorylmutase (EC 2.7.8.23), at 5,042,654 to 5,043,505 _4570 Pf1N1B4_4571 - ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63), at 5,043,657 to 5,044,589 _4571 Pf1N1B4_4572 - hypothetical protein, at 5,044,713 to 5,045,039 _4572 Pf1N1B4_4573 - transporter, LysE family, at 5,045,032 to 5,045,739 _4573 Position (kb) 5043 5044 5045Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 5042.736 kb on + strandat 5042.952 kb on + strand, within Pf1N1B4_4570at 5042.953 kb on - strand, within Pf1N1B4_4570at 5042.953 kb on - strand, within Pf1N1B4_4570at 5043.004 kb on - strand, within Pf1N1B4_4570at 5043.066 kb on + strand, within Pf1N1B4_4570at 5043.067 kb on - strand, within Pf1N1B4_4570at 5043.067 kb on - strand, within Pf1N1B4_4570at 5043.338 kb on - strand, within Pf1N1B4_4570at 5043.760 kb on + strand, within Pf1N1B4_4571at 5043.760 kb on + strand, within Pf1N1B4_4571at 5043.760 kb on + strand, within Pf1N1B4_4571at 5043.765 kb on - strand, within Pf1N1B4_4571at 5043.765 kb on - strand, within Pf1N1B4_4571at 5043.875 kb on - strand, within Pf1N1B4_4571at 5044.639 kb on + strandat 5044.639 kb on + strandat 5044.639 kb on + strandat 5044.640 kb on - strandat 5044.640 kb on - strandat 5044.640 kb on - strandat 5044.640 kb on - strandat 5044.640 kb on - strandat 5044.739 kb on + strandat 5044.740 kb on - strandat 5044.820 kb on + strand, within Pf1N1B4_4572at 5045.112 kb on + strandat 5045.113 kb on - strand, within Pf1N1B4_4573at 5045.113 kb on - strand, within Pf1N1B4_4573at 5045.113 kb on - strandat 5045.292 kb on - strand, within Pf1N1B4_4573at 5045.292 kb on - strand, within Pf1N1B4_4573at 5045.537 kb on + strand, within Pf1N1B4_4573at 5045.537 kb on + strand, within Pf1N1B4_4573at 5045.538 kb on - strand, within Pf1N1B4_4573at 5045.538 kb on - strand, within Pf1N1B4_4573at 5045.539 kb on + strand, within Pf1N1B4_4573at 5045.540 kb on - strand, within Pf1N1B4_4573at 5045.574 kb on - strand, within Pf1N1B4_4573at 5045.574 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction
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5,042,736 + -0.1
5,042,952 + Pf1N1B4_4570 0.35 -1.4
5,042,953 - Pf1N1B4_4570 0.35 -0.1
5,042,953 - Pf1N1B4_4570 0.35 -0.1
5,043,004 - Pf1N1B4_4570 0.41 -3.4
5,043,066 + Pf1N1B4_4570 0.48 -0.7
5,043,067 - Pf1N1B4_4570 0.48 -0.1
5,043,067 - Pf1N1B4_4570 0.48 -0.1
5,043,338 - Pf1N1B4_4570 0.80 +0.7
5,043,760 + Pf1N1B4_4571 0.11 -0.1
5,043,760 + Pf1N1B4_4571 0.11 -0.1
5,043,760 + Pf1N1B4_4571 0.11 -4.7
5,043,765 - Pf1N1B4_4571 0.12 -0.1
5,043,765 - Pf1N1B4_4571 0.12 -0.1
5,043,875 - Pf1N1B4_4571 0.23 -0.1
5,044,639 + -0.1
5,044,639 + -1.1
5,044,639 + -0.1
5,044,640 - -1.9
5,044,640 - -3.5
5,044,640 - -0.1
5,044,640 - -4.2
5,044,640 - -0.1
5,044,739 + -2.5
5,044,740 - -0.1
5,044,820 + Pf1N1B4_4572 0.33 -0.1
5,045,112 + -2.8
5,045,113 - Pf1N1B4_4573 0.11 -0.1
5,045,113 - Pf1N1B4_4573 0.11 -0.1
5,045,113 - -0.1
5,045,292 - Pf1N1B4_4573 0.37 -0.1
5,045,292 - Pf1N1B4_4573 0.37 -0.1
5,045,537 + Pf1N1B4_4573 0.71 -0.2
5,045,537 + Pf1N1B4_4573 0.71 +1.4
5,045,538 - Pf1N1B4_4573 0.71 -0.5
5,045,538 - Pf1N1B4_4573 0.71 -1.4
5,045,539 + Pf1N1B4_4573 0.72 -2.9
5,045,540 - Pf1N1B4_4573 0.72 -0.1
5,045,574 - Pf1N1B4_4573 0.77 -0.1
5,045,574 - -0.1

Or see this region's nucleotide sequence