Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4433

Experiment: Nitrate reduction

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4431 and Pf1N1B4_4432 are separated by 61 nucleotidesPf1N1B4_4432 and Pf1N1B4_4433 are separated by 47 nucleotidesPf1N1B4_4433 and Pf1N1B4_4434 are separated by 18 nucleotidesPf1N1B4_4434 and Pf1N1B4_4435 overlap by 4 nucleotidesPf1N1B4_4435 and Pf1N1B4_4436 are separated by 229 nucleotides Pf1N1B4_4431 - Permeases of the major facilitator superfamily, at 4,892,674 to 4,893,861 _4431 Pf1N1B4_4432 - Dienelactone hydrolase family, at 4,893,923 to 4,894,621 _4432 Pf1N1B4_4433 - FIG035246: DoxX family protein, at 4,894,669 to 4,895,184 _4433 Pf1N1B4_4434 - hypothetical protein, at 4,895,203 to 4,895,325 _4434 Pf1N1B4_4435 - Inner membrane protein, at 4,895,322 to 4,895,951 _4435 Pf1N1B4_4436 - Glutaminase (EC 3.5.1.2), at 4,896,181 to 4,897,089 _4436 Position (kb) 4894 4895 4896Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 4893.761 kb on - strandat 4893.823 kb on - strandat 4893.896 kb on + strandat 4893.896 kb on + strandat 4893.897 kb on - strandat 4893.897 kb on - strandat 4893.925 kb on - strandat 4893.952 kb on + strandat 4893.952 kb on + strandat 4893.953 kb on - strandat 4893.953 kb on - strandat 4893.987 kb on + strandat 4893.987 kb on + strandat 4893.988 kb on - strandat 4894.233 kb on + strand, within Pf1N1B4_4432at 4894.467 kb on + strand, within Pf1N1B4_4432at 4894.467 kb on + strand, within Pf1N1B4_4432at 4894.468 kb on - strand, within Pf1N1B4_4432at 4894.468 kb on - strand, within Pf1N1B4_4432at 4894.634 kb on - strandat 4894.634 kb on - strandat 4894.634 kb on - strandat 4894.637 kb on + strandat 4894.637 kb on + strandat 4894.637 kb on + strandat 4894.637 kb on + strandat 4894.637 kb on + strandat 4894.638 kb on - strandat 4894.638 kb on - strandat 4894.767 kb on - strand, within Pf1N1B4_4433at 4894.767 kb on - strand, within Pf1N1B4_4433at 4894.801 kb on - strand, within Pf1N1B4_4433at 4895.178 kb on + strandat 4895.178 kb on + strandat 4895.179 kb on - strandat 4895.179 kb on - strandat 4895.429 kb on - strandat 4895.429 kb on - strand, within Pf1N1B4_4435at 4895.429 kb on - strand, within Pf1N1B4_4435at 4895.429 kb on - strand, within Pf1N1B4_4435at 4895.429 kb on - strand, within Pf1N1B4_4435at 4895.653 kb on + strand, within Pf1N1B4_4435at 4895.659 kb on + strand, within Pf1N1B4_4435at 4895.660 kb on - strand, within Pf1N1B4_4435at 4895.660 kb on - strandat 4895.660 kb on - strand, within Pf1N1B4_4435at 4895.660 kb on - strand, within Pf1N1B4_4435at 4896.087 kb on + strandat 4896.087 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction
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4,893,761 - -1.6
4,893,823 - -0.2
4,893,896 + -0.2
4,893,896 + -0.2
4,893,897 - -0.2
4,893,897 - -0.2
4,893,925 - -0.6
4,893,952 + -1.8
4,893,952 + -3.7
4,893,953 - -0.2
4,893,953 - -0.7
4,893,987 + -0.2
4,893,987 + -0.2
4,893,988 - -0.2
4,894,233 + Pf1N1B4_4432 0.44 -0.2
4,894,467 + Pf1N1B4_4432 0.78 -3.0
4,894,467 + Pf1N1B4_4432 0.78 -0.9
4,894,468 - Pf1N1B4_4432 0.78 -2.2
4,894,468 - Pf1N1B4_4432 0.78 -0.4
4,894,634 - -0.2
4,894,634 - -0.2
4,894,634 - -0.2
4,894,637 + -0.2
4,894,637 + -0.2
4,894,637 + -0.2
4,894,637 + -0.4
4,894,637 + -0.4
4,894,638 - -0.2
4,894,638 - -0.2
4,894,767 - Pf1N1B4_4433 0.19 -0.2
4,894,767 - Pf1N1B4_4433 0.19 -0.2
4,894,801 - Pf1N1B4_4433 0.26 -2.7
4,895,178 + -0.2
4,895,178 + -4.6
4,895,179 - -1.2
4,895,179 - -1.3
4,895,429 - -1.2
4,895,429 - Pf1N1B4_4435 0.17 -3.0
4,895,429 - Pf1N1B4_4435 0.17 -1.9
4,895,429 - Pf1N1B4_4435 0.17 -4.2
4,895,429 - Pf1N1B4_4435 0.17 -0.2
4,895,653 + Pf1N1B4_4435 0.53 +0.3
4,895,659 + Pf1N1B4_4435 0.53 -0.2
4,895,660 - Pf1N1B4_4435 0.54 -2.0
4,895,660 - -0.2
4,895,660 - Pf1N1B4_4435 0.54 -0.2
4,895,660 - Pf1N1B4_4435 0.54 -0.2
4,896,087 + -0.2
4,896,087 + -0.2

Or see this region's nucleotide sequence