Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4431 and Pf1N1B4_4432 are separated by 61 nucleotides Pf1N1B4_4432 and Pf1N1B4_4433 are separated by 47 nucleotides Pf1N1B4_4433 and Pf1N1B4_4434 are separated by 18 nucleotides Pf1N1B4_4434 and Pf1N1B4_4435 overlap by 4 nucleotides Pf1N1B4_4435 and Pf1N1B4_4436 are separated by 229 nucleotides
Pf1N1B4_4431 - Permeases of the major facilitator superfamily, at 4,892,674 to 4,893,861
_4431
Pf1N1B4_4432 - Dienelactone hydrolase family, at 4,893,923 to 4,894,621
_4432
Pf1N1B4_4433 - FIG035246: DoxX family protein, at 4,894,669 to 4,895,184
_4433
Pf1N1B4_4434 - hypothetical protein, at 4,895,203 to 4,895,325
_4434
Pf1N1B4_4435 - Inner membrane protein, at 4,895,322 to 4,895,951
_4435
Pf1N1B4_4436 - Glutaminase (EC 3.5.1.2), at 4,896,181 to 4,897,089
_4436
Position (kb)
4894
4895
4896 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4893.761 kb on - strand at 4893.823 kb on - strand at 4893.896 kb on + strand at 4893.896 kb on + strand at 4893.897 kb on - strand at 4893.897 kb on - strand at 4893.925 kb on - strand at 4893.952 kb on + strand at 4893.952 kb on + strand at 4893.953 kb on - strand at 4893.953 kb on - strand at 4893.987 kb on + strand at 4893.987 kb on + strand at 4893.988 kb on - strand at 4894.233 kb on + strand, within Pf1N1B4_4432 at 4894.467 kb on + strand, within Pf1N1B4_4432 at 4894.467 kb on + strand, within Pf1N1B4_4432 at 4894.468 kb on - strand, within Pf1N1B4_4432 at 4894.468 kb on - strand, within Pf1N1B4_4432 at 4894.634 kb on - strand at 4894.634 kb on - strand at 4894.634 kb on - strand at 4894.637 kb on + strand at 4894.637 kb on + strand at 4894.637 kb on + strand at 4894.637 kb on + strand at 4894.637 kb on + strand at 4894.638 kb on - strand at 4894.638 kb on - strand at 4894.767 kb on - strand, within Pf1N1B4_4433 at 4894.767 kb on - strand, within Pf1N1B4_4433 at 4894.801 kb on - strand, within Pf1N1B4_4433 at 4895.178 kb on + strand at 4895.178 kb on + strand at 4895.179 kb on - strand at 4895.179 kb on - strand at 4895.429 kb on - strand at 4895.429 kb on - strand, within Pf1N1B4_4435 at 4895.429 kb on - strand, within Pf1N1B4_4435 at 4895.429 kb on - strand, within Pf1N1B4_4435 at 4895.429 kb on - strand, within Pf1N1B4_4435 at 4895.653 kb on + strand, within Pf1N1B4_4435 at 4895.659 kb on + strand, within Pf1N1B4_4435 at 4895.660 kb on - strand, within Pf1N1B4_4435 at 4895.660 kb on - strand at 4895.660 kb on - strand, within Pf1N1B4_4435 at 4895.660 kb on - strand, within Pf1N1B4_4435 at 4896.087 kb on + strand at 4896.087 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 4,893,761 - -1.6 4,893,823 - -0.2 4,893,896 + -0.2 4,893,896 + -0.2 4,893,897 - -0.2 4,893,897 - -0.2 4,893,925 - -0.6 4,893,952 + -1.8 4,893,952 + -3.7 4,893,953 - -0.2 4,893,953 - -0.7 4,893,987 + -0.2 4,893,987 + -0.2 4,893,988 - -0.2 4,894,233 + Pf1N1B4_4432 0.44 -0.2 4,894,467 + Pf1N1B4_4432 0.78 -3.0 4,894,467 + Pf1N1B4_4432 0.78 -0.9 4,894,468 - Pf1N1B4_4432 0.78 -2.2 4,894,468 - Pf1N1B4_4432 0.78 -0.4 4,894,634 - -0.2 4,894,634 - -0.2 4,894,634 - -0.2 4,894,637 + -0.2 4,894,637 + -0.2 4,894,637 + -0.2 4,894,637 + -0.4 4,894,637 + -0.4 4,894,638 - -0.2 4,894,638 - -0.2 4,894,767 - Pf1N1B4_4433 0.19 -0.2 4,894,767 - Pf1N1B4_4433 0.19 -0.2 4,894,801 - Pf1N1B4_4433 0.26 -2.7 4,895,178 + -0.2 4,895,178 + -4.6 4,895,179 - -1.2 4,895,179 - -1.3 4,895,429 - -1.2 4,895,429 - Pf1N1B4_4435 0.17 -3.0 4,895,429 - Pf1N1B4_4435 0.17 -1.9 4,895,429 - Pf1N1B4_4435 0.17 -4.2 4,895,429 - Pf1N1B4_4435 0.17 -0.2 4,895,653 + Pf1N1B4_4435 0.53 +0.3 4,895,659 + Pf1N1B4_4435 0.53 -0.2 4,895,660 - Pf1N1B4_4435 0.54 -2.0 4,895,660 - -0.2 4,895,660 - Pf1N1B4_4435 0.54 -0.2 4,895,660 - Pf1N1B4_4435 0.54 -0.2 4,896,087 + -0.2 4,896,087 + -0.2
Or see this region's nucleotide sequence