Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4342 and Pf1N1B4_4343 are separated by 27 nucleotides Pf1N1B4_4343 and Pf1N1B4_4344 overlap by 4 nucleotides Pf1N1B4_4344 and Pf1N1B4_4345 are separated by 317 nucleotides Pf1N1B4_4345 and Pf1N1B4_4346 are separated by 17 nucleotides Pf1N1B4_4346 and Pf1N1B4_4347 are separated by 14 nucleotides Pf1N1B4_4347 and Pf1N1B4_4348 are separated by 124 nucleotides
Pf1N1B4_4342 - hypothetical protein, at 4,801,385 to 4,801,528
_4342
Pf1N1B4_4343 - hypothetical protein, at 4,801,556 to 4,801,825
_4343
Pf1N1B4_4344 - hypothetical protein, at 4,801,822 to 4,801,977
_4344
Pf1N1B4_4345 - hypothetical protein, at 4,802,295 to 4,803,356
_4345
Pf1N1B4_4346 - Protein of unknown function DUF1428, at 4,803,374 to 4,803,727
_4346
Pf1N1B4_4347 - hypothetical protein, at 4,803,742 to 4,803,936
_4347
Pf1N1B4_4348 - 2'-5' RNA ligase, at 4,804,061 to 4,804,600
_4348
Position (kb)
4802
4803
4804 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 4801.343 kb on + strand at 4801.347 kb on + strand at 4801.348 kb on - strand at 4801.359 kb on + strand at 4801.359 kb on + strand at 4801.359 kb on + strand at 4801.359 kb on + strand at 4801.359 kb on + strand at 4801.360 kb on - strand at 4801.360 kb on - strand at 4801.380 kb on + strand at 4801.396 kb on - strand at 4801.614 kb on - strand, within Pf1N1B4_4343 at 4801.770 kb on + strand, within Pf1N1B4_4343 at 4801.773 kb on + strand, within Pf1N1B4_4343 at 4801.773 kb on + strand, within Pf1N1B4_4343 at 4801.773 kb on + strand at 4801.773 kb on + strand, within Pf1N1B4_4343 at 4801.773 kb on + strand, within Pf1N1B4_4343 at 4801.773 kb on + strand, within Pf1N1B4_4343 at 4801.774 kb on - strand, within Pf1N1B4_4343 at 4801.787 kb on + strand, within Pf1N1B4_4343 at 4801.955 kb on + strand, within Pf1N1B4_4344 at 4802.015 kb on + strand at 4802.015 kb on + strand at 4802.016 kb on - strand at 4802.019 kb on + strand at 4802.059 kb on + strand at 4802.059 kb on + strand at 4802.060 kb on - strand at 4802.139 kb on + strand at 4802.139 kb on + strand at 4802.142 kb on - strand at 4802.226 kb on + strand at 4802.226 kb on + strand at 4802.226 kb on + strand at 4802.226 kb on + strand at 4802.227 kb on - strand at 4802.227 kb on - strand at 4802.227 kb on - strand at 4802.281 kb on - strand at 4802.480 kb on + strand, within Pf1N1B4_4345 at 4802.480 kb on + strand at 4802.480 kb on + strand, within Pf1N1B4_4345 at 4802.481 kb on - strand, within Pf1N1B4_4345 at 4802.655 kb on + strand, within Pf1N1B4_4345 at 4802.762 kb on + strand, within Pf1N1B4_4345 at 4803.008 kb on - strand, within Pf1N1B4_4345 at 4803.113 kb on + strand, within Pf1N1B4_4345 at 4803.237 kb on + strand, within Pf1N1B4_4345 at 4803.238 kb on - strand, within Pf1N1B4_4345 at 4803.238 kb on - strand, within Pf1N1B4_4345 at 4803.238 kb on - strand at 4803.380 kb on + strand at 4803.380 kb on + strand at 4803.381 kb on - strand at 4803.381 kb on - strand at 4803.405 kb on + strand at 4803.546 kb on + strand, within Pf1N1B4_4346 at 4803.546 kb on + strand, within Pf1N1B4_4346 at 4803.547 kb on - strand, within Pf1N1B4_4346 at 4803.843 kb on - strand at 4803.843 kb on - strand, within Pf1N1B4_4347 at 4803.843 kb on - strand, within Pf1N1B4_4347 at 4803.968 kb on + strand at 4803.968 kb on + strand at 4803.969 kb on - strand at 4803.969 kb on - strand at 4803.970 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 4,801,343 + -0.2 4,801,347 + -0.2 4,801,348 - -0.8 4,801,359 + -0.2 4,801,359 + -0.2 4,801,359 + -0.2 4,801,359 + -3.8 4,801,359 + -3.5 4,801,360 - -0.2 4,801,360 - -5.1 4,801,380 + -1.6 4,801,396 - -0.2 4,801,614 - Pf1N1B4_4343 0.21 -0.2 4,801,770 + Pf1N1B4_4343 0.79 -0.2 4,801,773 + Pf1N1B4_4343 0.80 -0.2 4,801,773 + Pf1N1B4_4343 0.80 -0.2 4,801,773 + -0.2 4,801,773 + Pf1N1B4_4343 0.80 -0.2 4,801,773 + Pf1N1B4_4343 0.80 -0.2 4,801,773 + Pf1N1B4_4343 0.80 -0.2 4,801,774 - Pf1N1B4_4343 0.81 -3.9 4,801,787 + Pf1N1B4_4343 0.86 -4.9 4,801,955 + Pf1N1B4_4344 0.85 -0.2 4,802,015 + -0.2 4,802,015 + -2.0 4,802,016 - +1.4 4,802,019 + -0.2 4,802,059 + -0.2 4,802,059 + -0.2 4,802,060 - -3.9 4,802,139 + -0.2 4,802,139 + -0.2 4,802,142 - -0.2 4,802,226 + -0.2 4,802,226 + -0.2 4,802,226 + -0.2 4,802,226 + -4.2 4,802,227 - -1.7 4,802,227 - -0.2 4,802,227 - -0.2 4,802,281 - -1.7 4,802,480 + Pf1N1B4_4345 0.17 -0.2 4,802,480 + -0.2 4,802,480 + Pf1N1B4_4345 0.17 -0.2 4,802,481 - Pf1N1B4_4345 0.18 -5.1 4,802,655 + Pf1N1B4_4345 0.34 -2.5 4,802,762 + Pf1N1B4_4345 0.44 -0.2 4,803,008 - Pf1N1B4_4345 0.67 -4.0 4,803,113 + Pf1N1B4_4345 0.77 -2.2 4,803,237 + Pf1N1B4_4345 0.89 -2.1 4,803,238 - Pf1N1B4_4345 0.89 -0.2 4,803,238 - Pf1N1B4_4345 0.89 -0.2 4,803,238 - -2.7 4,803,380 + -4.0 4,803,380 + -0.2 4,803,381 - -0.2 4,803,381 - -0.2 4,803,405 + -0.2 4,803,546 + Pf1N1B4_4346 0.49 -0.2 4,803,546 + Pf1N1B4_4346 0.49 -0.2 4,803,547 - Pf1N1B4_4346 0.49 -0.2 4,803,843 - -0.2 4,803,843 - Pf1N1B4_4347 0.52 -0.2 4,803,843 - Pf1N1B4_4347 0.52 -0.2 4,803,968 + -1.9 4,803,968 + -0.2 4,803,969 - -0.2 4,803,969 - -0.2 4,803,970 + -1.5
Or see this region's nucleotide sequence