Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4345

Experiment: Nitrate reduction

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4342 and Pf1N1B4_4343 are separated by 27 nucleotidesPf1N1B4_4343 and Pf1N1B4_4344 overlap by 4 nucleotidesPf1N1B4_4344 and Pf1N1B4_4345 are separated by 317 nucleotidesPf1N1B4_4345 and Pf1N1B4_4346 are separated by 17 nucleotidesPf1N1B4_4346 and Pf1N1B4_4347 are separated by 14 nucleotidesPf1N1B4_4347 and Pf1N1B4_4348 are separated by 124 nucleotides Pf1N1B4_4342 - hypothetical protein, at 4,801,385 to 4,801,528 _4342 Pf1N1B4_4343 - hypothetical protein, at 4,801,556 to 4,801,825 _4343 Pf1N1B4_4344 - hypothetical protein, at 4,801,822 to 4,801,977 _4344 Pf1N1B4_4345 - hypothetical protein, at 4,802,295 to 4,803,356 _4345 Pf1N1B4_4346 - Protein of unknown function DUF1428, at 4,803,374 to 4,803,727 _4346 Pf1N1B4_4347 - hypothetical protein, at 4,803,742 to 4,803,936 _4347 Pf1N1B4_4348 - 2'-5' RNA ligase, at 4,804,061 to 4,804,600 _4348 Position (kb) 4802 4803 4804Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 4801.343 kb on + strandat 4801.347 kb on + strandat 4801.348 kb on - strandat 4801.359 kb on + strandat 4801.359 kb on + strandat 4801.359 kb on + strandat 4801.359 kb on + strandat 4801.359 kb on + strandat 4801.360 kb on - strandat 4801.360 kb on - strandat 4801.380 kb on + strandat 4801.396 kb on - strandat 4801.614 kb on - strand, within Pf1N1B4_4343at 4801.770 kb on + strand, within Pf1N1B4_4343at 4801.773 kb on + strand, within Pf1N1B4_4343at 4801.773 kb on + strand, within Pf1N1B4_4343at 4801.773 kb on + strandat 4801.773 kb on + strand, within Pf1N1B4_4343at 4801.773 kb on + strand, within Pf1N1B4_4343at 4801.773 kb on + strand, within Pf1N1B4_4343at 4801.774 kb on - strand, within Pf1N1B4_4343at 4801.787 kb on + strand, within Pf1N1B4_4343at 4801.955 kb on + strand, within Pf1N1B4_4344at 4802.015 kb on + strandat 4802.015 kb on + strandat 4802.016 kb on - strandat 4802.019 kb on + strandat 4802.059 kb on + strandat 4802.059 kb on + strandat 4802.060 kb on - strandat 4802.139 kb on + strandat 4802.139 kb on + strandat 4802.142 kb on - strandat 4802.226 kb on + strandat 4802.226 kb on + strandat 4802.226 kb on + strandat 4802.226 kb on + strandat 4802.227 kb on - strandat 4802.227 kb on - strandat 4802.227 kb on - strandat 4802.281 kb on - strandat 4802.480 kb on + strand, within Pf1N1B4_4345at 4802.480 kb on + strandat 4802.480 kb on + strand, within Pf1N1B4_4345at 4802.481 kb on - strand, within Pf1N1B4_4345at 4802.655 kb on + strand, within Pf1N1B4_4345at 4802.762 kb on + strand, within Pf1N1B4_4345at 4803.008 kb on - strand, within Pf1N1B4_4345at 4803.113 kb on + strand, within Pf1N1B4_4345at 4803.237 kb on + strand, within Pf1N1B4_4345at 4803.238 kb on - strand, within Pf1N1B4_4345at 4803.238 kb on - strand, within Pf1N1B4_4345at 4803.238 kb on - strandat 4803.380 kb on + strandat 4803.380 kb on + strandat 4803.381 kb on - strandat 4803.381 kb on - strandat 4803.405 kb on + strandat 4803.546 kb on + strand, within Pf1N1B4_4346at 4803.546 kb on + strand, within Pf1N1B4_4346at 4803.547 kb on - strand, within Pf1N1B4_4346at 4803.843 kb on - strandat 4803.843 kb on - strand, within Pf1N1B4_4347at 4803.843 kb on - strand, within Pf1N1B4_4347at 4803.968 kb on + strandat 4803.968 kb on + strandat 4803.969 kb on - strandat 4803.969 kb on - strandat 4803.970 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction
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4,801,343 + -0.2
4,801,347 + -0.2
4,801,348 - -0.8
4,801,359 + -0.2
4,801,359 + -0.2
4,801,359 + -0.2
4,801,359 + -3.8
4,801,359 + -3.5
4,801,360 - -0.2
4,801,360 - -5.1
4,801,380 + -1.6
4,801,396 - -0.2
4,801,614 - Pf1N1B4_4343 0.21 -0.2
4,801,770 + Pf1N1B4_4343 0.79 -0.2
4,801,773 + Pf1N1B4_4343 0.80 -0.2
4,801,773 + Pf1N1B4_4343 0.80 -0.2
4,801,773 + -0.2
4,801,773 + Pf1N1B4_4343 0.80 -0.2
4,801,773 + Pf1N1B4_4343 0.80 -0.2
4,801,773 + Pf1N1B4_4343 0.80 -0.2
4,801,774 - Pf1N1B4_4343 0.81 -3.9
4,801,787 + Pf1N1B4_4343 0.86 -4.9
4,801,955 + Pf1N1B4_4344 0.85 -0.2
4,802,015 + -0.2
4,802,015 + -2.0
4,802,016 - +1.4
4,802,019 + -0.2
4,802,059 + -0.2
4,802,059 + -0.2
4,802,060 - -3.9
4,802,139 + -0.2
4,802,139 + -0.2
4,802,142 - -0.2
4,802,226 + -0.2
4,802,226 + -0.2
4,802,226 + -0.2
4,802,226 + -4.2
4,802,227 - -1.7
4,802,227 - -0.2
4,802,227 - -0.2
4,802,281 - -1.7
4,802,480 + Pf1N1B4_4345 0.17 -0.2
4,802,480 + -0.2
4,802,480 + Pf1N1B4_4345 0.17 -0.2
4,802,481 - Pf1N1B4_4345 0.18 -5.1
4,802,655 + Pf1N1B4_4345 0.34 -2.5
4,802,762 + Pf1N1B4_4345 0.44 -0.2
4,803,008 - Pf1N1B4_4345 0.67 -4.0
4,803,113 + Pf1N1B4_4345 0.77 -2.2
4,803,237 + Pf1N1B4_4345 0.89 -2.1
4,803,238 - Pf1N1B4_4345 0.89 -0.2
4,803,238 - Pf1N1B4_4345 0.89 -0.2
4,803,238 - -2.7
4,803,380 + -4.0
4,803,380 + -0.2
4,803,381 - -0.2
4,803,381 - -0.2
4,803,405 + -0.2
4,803,546 + Pf1N1B4_4346 0.49 -0.2
4,803,546 + Pf1N1B4_4346 0.49 -0.2
4,803,547 - Pf1N1B4_4346 0.49 -0.2
4,803,843 - -0.2
4,803,843 - Pf1N1B4_4347 0.52 -0.2
4,803,843 - Pf1N1B4_4347 0.52 -0.2
4,803,968 + -1.9
4,803,968 + -0.2
4,803,969 - -0.2
4,803,969 - -0.2
4,803,970 + -1.5

Or see this region's nucleotide sequence