Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4341

Experiment: Nitrate reduction

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4339 and Pf1N1B4_4340 are separated by 37 nucleotidesPf1N1B4_4340 and Pf1N1B4_4341 are separated by 103 nucleotidesPf1N1B4_4341 and Pf1N1B4_4342 are separated by 364 nucleotidesPf1N1B4_4342 and Pf1N1B4_4343 are separated by 27 nucleotidesPf1N1B4_4343 and Pf1N1B4_4344 overlap by 4 nucleotides Pf1N1B4_4339 - Malic enzyme, at 4,798,331 to 4,799,551 _4339 Pf1N1B4_4340 - FOG: WD40-like repeat, at 4,799,589 to 4,800,218 _4340 Pf1N1B4_4341 - hypothetical protein, at 4,800,322 to 4,801,020 _4341 Pf1N1B4_4342 - hypothetical protein, at 4,801,385 to 4,801,528 _4342 Pf1N1B4_4343 - hypothetical protein, at 4,801,556 to 4,801,825 _4343 Pf1N1B4_4344 - hypothetical protein, at 4,801,822 to 4,801,977 _4344 Position (kb) 4800 4801 4802Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 4799.517 kb on + strandat 4799.518 kb on - strandat 4799.524 kb on - strandat 4799.550 kb on - strandat 4799.622 kb on + strandat 4799.845 kb on + strandat 4799.845 kb on + strand, within Pf1N1B4_4340at 4800.031 kb on + strand, within Pf1N1B4_4340at 4800.032 kb on - strand, within Pf1N1B4_4340at 4800.453 kb on + strand, within Pf1N1B4_4341at 4800.454 kb on - strandat 4800.578 kb on + strand, within Pf1N1B4_4341at 4800.610 kb on + strand, within Pf1N1B4_4341at 4800.611 kb on - strand, within Pf1N1B4_4341at 4800.616 kb on + strand, within Pf1N1B4_4341at 4800.616 kb on + strand, within Pf1N1B4_4341at 4800.616 kb on + strand, within Pf1N1B4_4341at 4800.616 kb on + strand, within Pf1N1B4_4341at 4800.631 kb on + strandat 4800.632 kb on - strand, within Pf1N1B4_4341at 4800.701 kb on - strand, within Pf1N1B4_4341at 4800.724 kb on + strandat 4800.724 kb on + strand, within Pf1N1B4_4341at 4800.725 kb on - strand, within Pf1N1B4_4341at 4801.228 kb on + strandat 4801.238 kb on + strandat 4801.238 kb on + strandat 4801.274 kb on + strandat 4801.343 kb on + strandat 4801.347 kb on + strandat 4801.348 kb on - strandat 4801.359 kb on + strandat 4801.359 kb on + strandat 4801.359 kb on + strandat 4801.359 kb on + strandat 4801.359 kb on + strandat 4801.360 kb on - strandat 4801.360 kb on - strandat 4801.380 kb on + strandat 4801.396 kb on - strandat 4801.614 kb on - strand, within Pf1N1B4_4343at 4801.770 kb on + strand, within Pf1N1B4_4343at 4801.773 kb on + strand, within Pf1N1B4_4343at 4801.773 kb on + strand, within Pf1N1B4_4343at 4801.773 kb on + strandat 4801.773 kb on + strand, within Pf1N1B4_4343at 4801.773 kb on + strand, within Pf1N1B4_4343at 4801.773 kb on + strand, within Pf1N1B4_4343at 4801.774 kb on - strand, within Pf1N1B4_4343at 4801.787 kb on + strand, within Pf1N1B4_4343at 4801.955 kb on + strand, within Pf1N1B4_4344at 4802.015 kb on + strandat 4802.015 kb on + strandat 4802.016 kb on - strandat 4802.019 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction
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4,799,517 + -0.2
4,799,518 - -0.2
4,799,524 - -1.9
4,799,550 - -0.4
4,799,622 + -3.5
4,799,845 + -1.4
4,799,845 + Pf1N1B4_4340 0.41 -0.2
4,800,031 + Pf1N1B4_4340 0.70 -0.2
4,800,032 - Pf1N1B4_4340 0.70 -0.2
4,800,453 + Pf1N1B4_4341 0.19 -0.2
4,800,454 - -2.0
4,800,578 + Pf1N1B4_4341 0.37 -0.2
4,800,610 + Pf1N1B4_4341 0.41 -0.2
4,800,611 - Pf1N1B4_4341 0.41 -3.3
4,800,616 + Pf1N1B4_4341 0.42 -0.2
4,800,616 + Pf1N1B4_4341 0.42 -0.2
4,800,616 + Pf1N1B4_4341 0.42 -0.2
4,800,616 + Pf1N1B4_4341 0.42 -1.2
4,800,631 + -1.5
4,800,632 - Pf1N1B4_4341 0.44 -4.5
4,800,701 - Pf1N1B4_4341 0.54 +0.6
4,800,724 + -0.2
4,800,724 + Pf1N1B4_4341 0.58 -0.2
4,800,725 - Pf1N1B4_4341 0.58 -0.2
4,801,228 + -0.2
4,801,238 + -0.2
4,801,238 + -0.2
4,801,274 + -2.2
4,801,343 + -0.2
4,801,347 + -0.2
4,801,348 - -0.8
4,801,359 + -0.2
4,801,359 + -0.2
4,801,359 + -0.2
4,801,359 + -3.8
4,801,359 + -3.5
4,801,360 - -0.2
4,801,360 - -5.1
4,801,380 + -1.6
4,801,396 - -0.2
4,801,614 - Pf1N1B4_4343 0.21 -0.2
4,801,770 + Pf1N1B4_4343 0.79 -0.2
4,801,773 + Pf1N1B4_4343 0.80 -0.2
4,801,773 + Pf1N1B4_4343 0.80 -0.2
4,801,773 + -0.2
4,801,773 + Pf1N1B4_4343 0.80 -0.2
4,801,773 + Pf1N1B4_4343 0.80 -0.2
4,801,773 + Pf1N1B4_4343 0.80 -0.2
4,801,774 - Pf1N1B4_4343 0.81 -3.9
4,801,787 + Pf1N1B4_4343 0.86 -4.9
4,801,955 + Pf1N1B4_4344 0.85 -0.2
4,802,015 + -0.2
4,802,015 + -2.0
4,802,016 - +1.4
4,802,019 + -0.2

Or see this region's nucleotide sequence