Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4076 and Pf1N1B4_4077 are separated by 147 nucleotides Pf1N1B4_4077 and Pf1N1B4_4078 are separated by 100 nucleotides Pf1N1B4_4078 and Pf1N1B4_4079 are separated by 784 nucleotides
Pf1N1B4_4076 - Histone acetyltransferase HPA2 and related acetyltransferases, at 4,526,467 to 4,526,895
_4076
Pf1N1B4_4077 - FIG00962160: hypothetical protein, at 4,527,043 to 4,527,480
_4077
Pf1N1B4_4078 - GDP-mannose pyrophosphatase YffH, at 4,527,581 to 4,528,174
_4078
Pf1N1B4_4079 - AraC family transcription regulator, at 4,528,959 to 4,529,660
_4079
Position (kb)
4527
4528
4529 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 4526.718 kb on - strand, within Pf1N1B4_4076 at 4526.733 kb on - strand, within Pf1N1B4_4076 at 4526.818 kb on - strand, within Pf1N1B4_4076 at 4526.820 kb on - strand at 4526.820 kb on - strand, within Pf1N1B4_4076 at 4526.820 kb on - strand, within Pf1N1B4_4076 at 4526.820 kb on - strand, within Pf1N1B4_4076 at 4526.895 kb on + strand at 4526.896 kb on - strand at 4526.896 kb on - strand at 4526.900 kb on + strand at 4526.901 kb on - strand at 4526.901 kb on - strand at 4526.998 kb on - strand at 4527.145 kb on + strand, within Pf1N1B4_4077 at 4527.146 kb on - strand, within Pf1N1B4_4077 at 4527.202 kb on + strand, within Pf1N1B4_4077 at 4527.203 kb on - strand at 4527.241 kb on - strand, within Pf1N1B4_4077 at 4527.448 kb on + strand at 4527.460 kb on + strand at 4527.461 kb on - strand at 4527.461 kb on - strand at 4527.565 kb on + strand at 4527.566 kb on - strand at 4527.641 kb on + strand, within Pf1N1B4_4078 at 4527.641 kb on + strand, within Pf1N1B4_4078 at 4527.669 kb on - strand, within Pf1N1B4_4078 at 4527.713 kb on + strand, within Pf1N1B4_4078 at 4527.941 kb on + strand, within Pf1N1B4_4078 at 4527.942 kb on - strand, within Pf1N1B4_4078 at 4527.942 kb on - strand, within Pf1N1B4_4078 at 4527.942 kb on - strand, within Pf1N1B4_4078 at 4528.094 kb on + strand at 4528.094 kb on + strand, within Pf1N1B4_4078 at 4528.095 kb on - strand, within Pf1N1B4_4078 at 4528.139 kb on + strand at 4528.314 kb on - strand at 4528.314 kb on - strand at 4528.451 kb on + strand at 4528.451 kb on + strand at 4528.452 kb on - strand at 4528.452 kb on - strand at 4528.500 kb on - strand at 4528.514 kb on + strand at 4528.514 kb on + strand at 4528.514 kb on + strand at 4528.515 kb on - strand at 4528.570 kb on - strand at 4528.629 kb on + strand at 4528.629 kb on + strand at 4528.629 kb on + strand at 4528.630 kb on - strand at 4528.690 kb on + strand at 4528.691 kb on - strand at 4528.777 kb on + strand at 4528.778 kb on - strand at 4528.778 kb on - strand at 4528.778 kb on - strand at 4528.779 kb on + strand at 4528.779 kb on + strand at 4528.779 kb on + strand at 4528.779 kb on + strand at 4528.779 kb on + strand at 4528.779 kb on + strand at 4528.779 kb on + strand at 4528.780 kb on - strand at 4528.780 kb on - strand at 4528.780 kb on - strand at 4528.780 kb on - strand at 4528.780 kb on - strand at 4528.780 kb on - strand at 4528.780 kb on - strand at 4528.815 kb on + strand at 4528.816 kb on - strand at 4528.816 kb on - strand at 4528.916 kb on + strand at 4528.916 kb on + strand at 4528.916 kb on + strand at 4528.916 kb on + strand at 4528.917 kb on - strand at 4529.085 kb on + strand, within Pf1N1B4_4079 at 4529.085 kb on + strand, within Pf1N1B4_4079 at 4529.086 kb on - strand, within Pf1N1B4_4079 at 4529.086 kb on - strand, within Pf1N1B4_4079 at 4529.086 kb on - strand, within Pf1N1B4_4079 at 4529.086 kb on - strand, within Pf1N1B4_4079 at 4529.086 kb on - strand, within Pf1N1B4_4079
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 4,526,718 - Pf1N1B4_4076 0.59 -0.1 4,526,733 - Pf1N1B4_4076 0.62 -0.1 4,526,818 - Pf1N1B4_4076 0.82 -0.1 4,526,820 - -0.1 4,526,820 - Pf1N1B4_4076 0.82 -0.1 4,526,820 - Pf1N1B4_4076 0.82 +0.6 4,526,820 - Pf1N1B4_4076 0.82 -2.6 4,526,895 + -0.1 4,526,896 - -0.1 4,526,896 - -0.1 4,526,900 + -0.1 4,526,901 - -0.1 4,526,901 - -1.1 4,526,998 - -0.1 4,527,145 + Pf1N1B4_4077 0.23 +0.3 4,527,146 - Pf1N1B4_4077 0.24 -0.1 4,527,202 + Pf1N1B4_4077 0.36 -0.1 4,527,203 - +1.2 4,527,241 - Pf1N1B4_4077 0.45 -0.1 4,527,448 + -1.3 4,527,460 + -0.1 4,527,461 - -1.0 4,527,461 - -0.1 4,527,565 + -0.1 4,527,566 - -0.1 4,527,641 + Pf1N1B4_4078 0.10 -4.6 4,527,641 + Pf1N1B4_4078 0.10 -2.2 4,527,669 - Pf1N1B4_4078 0.15 -0.1 4,527,713 + Pf1N1B4_4078 0.22 -2.6 4,527,941 + Pf1N1B4_4078 0.61 -0.1 4,527,942 - Pf1N1B4_4078 0.61 -0.1 4,527,942 - Pf1N1B4_4078 0.61 -0.1 4,527,942 - Pf1N1B4_4078 0.61 -0.1 4,528,094 + -0.1 4,528,094 + Pf1N1B4_4078 0.86 -0.1 4,528,095 - Pf1N1B4_4078 0.87 -4.0 4,528,139 + -0.1 4,528,314 - -1.5 4,528,314 - -0.1 4,528,451 + -4.9 4,528,451 + -3.2 4,528,452 - -0.1 4,528,452 - -0.1 4,528,500 - -0.5 4,528,514 + -0.2 4,528,514 + -2.1 4,528,514 + -0.1 4,528,515 - -0.1 4,528,570 - -0.1 4,528,629 + -2.7 4,528,629 + -0.1 4,528,629 + -0.1 4,528,630 - -2.1 4,528,690 + -2.2 4,528,691 - -0.1 4,528,777 + -0.1 4,528,778 - -0.1 4,528,778 - -0.1 4,528,778 - -0.5 4,528,779 + -0.1 4,528,779 + -0.1 4,528,779 + -3.4 4,528,779 + -0.1 4,528,779 + -2.3 4,528,779 + -0.1 4,528,779 + -1.3 4,528,780 - -0.1 4,528,780 - -0.1 4,528,780 - -0.1 4,528,780 - -0.1 4,528,780 - -0.1 4,528,780 - -0.1 4,528,780 - -1.5 4,528,815 + -0.1 4,528,816 - -1.9 4,528,816 - -5.4 4,528,916 + -2.3 4,528,916 + -0.1 4,528,916 + -2.1 4,528,916 + -0.1 4,528,917 - -1.5 4,529,085 + Pf1N1B4_4079 0.18 -2.0 4,529,085 + Pf1N1B4_4079 0.18 -0.1 4,529,086 - Pf1N1B4_4079 0.18 -0.1 4,529,086 - Pf1N1B4_4079 0.18 -0.1 4,529,086 - Pf1N1B4_4079 0.18 -0.1 4,529,086 - Pf1N1B4_4079 0.18 -0.1 4,529,086 - Pf1N1B4_4079 0.18 -2.0
Or see this region's nucleotide sequence