Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4059 and Pf1N1B4_4060 are separated by 306 nucleotides Pf1N1B4_4060 and Pf1N1B4_4061 are separated by 68 nucleotides Pf1N1B4_4061 and Pf1N1B4_4062 are separated by 172 nucleotides
Pf1N1B4_4059 - Thioredoxin reductase (EC 1.8.1.9), at 4,511,761 to 4,512,720
_4059
Pf1N1B4_4060 - Cell division protein FtsK, at 4,513,027 to 4,515,432
_4060
Pf1N1B4_4061 - Outer membrane lipoprotein carrier protein LolA, at 4,515,501 to 4,516,124
_4061
Pf1N1B4_4062 - FIG065221: Holliday junction DNA helicase, at 4,516,297 to 4,517,619
_4062
Position (kb)
4513
4514
4515
4516 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4512.088 kb on + strand, within Pf1N1B4_4059 at 4512.089 kb on - strand, within Pf1N1B4_4059 at 4512.230 kb on + strand, within Pf1N1B4_4059 at 4512.255 kb on + strand, within Pf1N1B4_4059 at 4512.277 kb on + strand, within Pf1N1B4_4059 at 4512.278 kb on - strand at 4512.365 kb on + strand, within Pf1N1B4_4059 at 4512.410 kb on + strand, within Pf1N1B4_4059 at 4512.410 kb on + strand, within Pf1N1B4_4059 at 4512.977 kb on + strand at 4515.488 kb on + strand at 4516.123 kb on - strand at 4516.136 kb on + strand at 4516.136 kb on + strand at 4516.136 kb on + strand at 4516.137 kb on - strand at 4516.137 kb on - strand at 4516.137 kb on - strand at 4516.175 kb on + strand at 4516.175 kb on + strand at 4516.175 kb on + strand at 4516.176 kb on - strand at 4516.288 kb on + strand at 4516.288 kb on + strand at 4516.288 kb on + strand at 4516.288 kb on + strand at 4516.288 kb on + strand at 4516.289 kb on - strand at 4516.289 kb on - strand at 4516.289 kb on - strand at 4516.293 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 4,512,088 + Pf1N1B4_4059 0.34 -0.1 4,512,089 - Pf1N1B4_4059 0.34 -0.1 4,512,230 + Pf1N1B4_4059 0.49 -0.3 4,512,255 + Pf1N1B4_4059 0.51 -2.4 4,512,277 + Pf1N1B4_4059 0.54 -0.4 4,512,278 - -2.7 4,512,365 + Pf1N1B4_4059 0.63 -0.1 4,512,410 + Pf1N1B4_4059 0.68 -0.1 4,512,410 + Pf1N1B4_4059 0.68 -0.1 4,512,977 + -0.1 4,515,488 + -0.1 4,516,123 - -0.1 4,516,136 + -0.1 4,516,136 + -0.5 4,516,136 + -0.1 4,516,137 - -4.0 4,516,137 - -1.5 4,516,137 - -3.2 4,516,175 + -0.9 4,516,175 + -2.4 4,516,175 + -0.1 4,516,176 - -1.0 4,516,288 + -0.1 4,516,288 + -1.8 4,516,288 + -0.1 4,516,288 + -1.9 4,516,288 + -1.0 4,516,289 - -0.1 4,516,289 - -0.8 4,516,289 - -0.1 4,516,293 - -0.1
Or see this region's nucleotide sequence