Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4019

Experiment: Nitrate reduction

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4018 and Pf1N1B4_4019 are separated by 49 nucleotidesPf1N1B4_4019 and Pf1N1B4_4020 are separated by 51 nucleotides Pf1N1B4_4018 - Heavy metal RND efflux outer membrane protein, CzcC family, at 4,469,028 to 4,470,287 _4018 Pf1N1B4_4019 - Cobalt/zinc/cadmium efflux RND transporter, membrane fusion protein, CzcB family, at 4,470,337 to 4,471,530 _4019 Pf1N1B4_4020 - Cobalt-zinc-cadmium resistance protein CzcA; Cation efflux system protein CusA, at 4,471,582 to 4,474,083 _4020 Position (kb) 4470 4471 4472Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 4469.871 kb on + strand, within Pf1N1B4_4018at 4469.872 kb on - strand, within Pf1N1B4_4018at 4470.208 kb on - strandat 4470.799 kb on + strand, within Pf1N1B4_4019at 4470.799 kb on + strand, within Pf1N1B4_4019at 4470.800 kb on - strand, within Pf1N1B4_4019at 4470.800 kb on - strandat 4470.920 kb on + strand, within Pf1N1B4_4019at 4470.921 kb on - strand, within Pf1N1B4_4019at 4470.921 kb on - strand, within Pf1N1B4_4019at 4471.581 kb on + strandat 4471.581 kb on + strandat 4471.581 kb on + strandat 4471.582 kb on - strandat 4471.582 kb on - strandat 4471.673 kb on - strandat 4472.054 kb on - strandat 4472.054 kb on - strand, within Pf1N1B4_4020at 4472.197 kb on + strand, within Pf1N1B4_4020

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction
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4,469,871 + Pf1N1B4_4018 0.67 -0.1
4,469,872 - Pf1N1B4_4018 0.67 -0.1
4,470,208 - -2.4
4,470,799 + Pf1N1B4_4019 0.39 -2.6
4,470,799 + Pf1N1B4_4019 0.39 -0.1
4,470,800 - Pf1N1B4_4019 0.39 -0.1
4,470,800 - -1.6
4,470,920 + Pf1N1B4_4019 0.49 -0.1
4,470,921 - Pf1N1B4_4019 0.49 -5.5
4,470,921 - Pf1N1B4_4019 0.49 -0.1
4,471,581 + -0.1
4,471,581 + -0.1
4,471,581 + -2.8
4,471,582 - -0.1
4,471,582 - -3.0
4,471,673 - -1.8
4,472,054 - +0.1
4,472,054 - Pf1N1B4_4020 0.19 -0.1
4,472,197 + Pf1N1B4_4020 0.25 -1.3

Or see this region's nucleotide sequence