Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3984

Experiment: Nitrate reduction

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3982 and Pf1N1B4_3983 are separated by 120 nucleotidesPf1N1B4_3983 and Pf1N1B4_3984 are separated by 39 nucleotidesPf1N1B4_3984 and Pf1N1B4_3985 overlap by 1 nucleotidesPf1N1B4_3985 and Pf1N1B4_3986 are separated by 115 nucleotides Pf1N1B4_3982 - FIG00953347: hypothetical protein, at 4,423,698 to 4,424,039 _3982 Pf1N1B4_3983 - cI, at 4,424,160 to 4,424,834 _3983 Pf1N1B4_3984 - 3-methylcrotonyl-CoA carboxylase, alpha (biotin-containing) subunit (EC 6.4.1.4) (from data), at 4,424,874 to 4,426,829 _3984 Pf1N1B4_3985 - Methylglutaconyl-CoA hydratase (EC 4.2.1.18), at 4,426,829 to 4,427,644 _3985 Pf1N1B4_3986 - Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4), at 4,427,760 to 4,429,367 _3986 Position (kb) 4424 4425 4426 4427Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 4423.942 kb on + strand, within Pf1N1B4_3982at 4423.942 kb on + strand, within Pf1N1B4_3982at 4423.943 kb on - strand, within Pf1N1B4_3982at 4423.943 kb on - strandat 4423.945 kb on + strand, within Pf1N1B4_3982at 4423.945 kb on + strand, within Pf1N1B4_3982at 4423.945 kb on + strand, within Pf1N1B4_3982at 4423.946 kb on - strand, within Pf1N1B4_3982at 4423.946 kb on - strand, within Pf1N1B4_3982at 4424.060 kb on + strandat 4424.060 kb on + strandat 4424.061 kb on - strandat 4424.085 kb on + strandat 4424.085 kb on + strandat 4424.086 kb on - strandat 4424.088 kb on + strandat 4424.088 kb on + strandat 4424.088 kb on + strandat 4424.089 kb on - strandat 4424.089 kb on - strandat 4424.089 kb on - strandat 4424.089 kb on - strandat 4424.092 kb on + strandat 4424.092 kb on + strandat 4424.092 kb on + strandat 4424.092 kb on + strandat 4424.092 kb on + strandat 4424.092 kb on + strandat 4424.092 kb on + strandat 4424.092 kb on + strandat 4424.093 kb on - strandat 4424.093 kb on - strandat 4424.093 kb on - strandat 4424.098 kb on - strandat 4424.121 kb on + strandat 4424.121 kb on + strandat 4424.156 kb on + strandat 4424.231 kb on - strandat 4424.231 kb on - strand, within Pf1N1B4_3983at 4424.282 kb on + strand, within Pf1N1B4_3983at 4424.282 kb on + strandat 4424.344 kb on - strand, within Pf1N1B4_3983at 4424.344 kb on - strand, within Pf1N1B4_3983at 4424.415 kb on + strand, within Pf1N1B4_3983at 4424.415 kb on + strand, within Pf1N1B4_3983at 4424.416 kb on - strandat 4424.482 kb on + strand, within Pf1N1B4_3983at 4424.538 kb on + strand, within Pf1N1B4_3983at 4424.807 kb on - strandat 4424.833 kb on - strandat 4424.935 kb on + strandat 4424.936 kb on - strandat 4424.936 kb on - strandat 4425.130 kb on + strand, within Pf1N1B4_3984at 4425.131 kb on - strand, within Pf1N1B4_3984at 4425.131 kb on - strand, within Pf1N1B4_3984at 4425.169 kb on + strand, within Pf1N1B4_3984at 4425.170 kb on - strand, within Pf1N1B4_3984at 4425.170 kb on - strand, within Pf1N1B4_3984at 4425.170 kb on - strand, within Pf1N1B4_3984at 4425.236 kb on - strand, within Pf1N1B4_3984at 4425.671 kb on - strand, within Pf1N1B4_3984at 4426.015 kb on + strand, within Pf1N1B4_3984at 4426.150 kb on + strand, within Pf1N1B4_3984at 4426.151 kb on - strand, within Pf1N1B4_3984at 4426.151 kb on - strand, within Pf1N1B4_3984at 4426.151 kb on - strand, within Pf1N1B4_3984at 4426.151 kb on - strand, within Pf1N1B4_3984at 4426.151 kb on - strand, within Pf1N1B4_3984at 4426.152 kb on + strand, within Pf1N1B4_3984at 4426.153 kb on - strand, within Pf1N1B4_3984at 4426.153 kb on - strand, within Pf1N1B4_3984at 4426.213 kb on + strand, within Pf1N1B4_3984at 4426.214 kb on - strand, within Pf1N1B4_3984at 4426.639 kb on + strandat 4426.640 kb on - strandat 4426.640 kb on - strandat 4427.321 kb on + strand, within Pf1N1B4_3985at 4427.361 kb on + strand, within Pf1N1B4_3985at 4427.361 kb on + strand, within Pf1N1B4_3985at 4427.361 kb on + strand, within Pf1N1B4_3985

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction
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4,423,942 + Pf1N1B4_3982 0.71 -0.1
4,423,942 + Pf1N1B4_3982 0.71 -1.4
4,423,943 - Pf1N1B4_3982 0.72 -0.1
4,423,943 - -2.3
4,423,945 + Pf1N1B4_3982 0.72 -0.1
4,423,945 + Pf1N1B4_3982 0.72 -0.1
4,423,945 + Pf1N1B4_3982 0.72 -2.0
4,423,946 - Pf1N1B4_3982 0.73 -0.1
4,423,946 - Pf1N1B4_3982 0.73 -2.0
4,424,060 + -0.1
4,424,060 + -5.3
4,424,061 - -0.1
4,424,085 + -0.1
4,424,085 + -0.1
4,424,086 - -0.1
4,424,088 + -1.5
4,424,088 + -3.6
4,424,088 + -0.1
4,424,089 - -0.1
4,424,089 - -1.6
4,424,089 - -0.1
4,424,089 - -0.1
4,424,092 + -0.1
4,424,092 + -3.9
4,424,092 + -2.3
4,424,092 + -1.8
4,424,092 + -0.7
4,424,092 + -0.1
4,424,092 + -1.0
4,424,092 + -1.5
4,424,093 - -0.1
4,424,093 - -0.1
4,424,093 - -4.6
4,424,098 - -3.3
4,424,121 + -0.1
4,424,121 + -0.1
4,424,156 + -3.2
4,424,231 - -2.2
4,424,231 - Pf1N1B4_3983 0.11 -0.1
4,424,282 + Pf1N1B4_3983 0.18 -0.1
4,424,282 + -0.1
4,424,344 - Pf1N1B4_3983 0.27 -1.6
4,424,344 - Pf1N1B4_3983 0.27 -0.1
4,424,415 + Pf1N1B4_3983 0.38 -0.1
4,424,415 + Pf1N1B4_3983 0.38 -0.1
4,424,416 - -2.5
4,424,482 + Pf1N1B4_3983 0.48 -0.1
4,424,538 + Pf1N1B4_3983 0.56 -0.1
4,424,807 - -0.1
4,424,833 - -0.8
4,424,935 + -1.8
4,424,936 - -0.1
4,424,936 - -1.2
4,425,130 + Pf1N1B4_3984 0.13 -0.1
4,425,131 - Pf1N1B4_3984 0.13 -0.1
4,425,131 - Pf1N1B4_3984 0.13 -0.1
4,425,169 + Pf1N1B4_3984 0.15 -3.2
4,425,170 - Pf1N1B4_3984 0.15 -0.1
4,425,170 - Pf1N1B4_3984 0.15 -0.1
4,425,170 - Pf1N1B4_3984 0.15 -5.5
4,425,236 - Pf1N1B4_3984 0.19 -0.1
4,425,671 - Pf1N1B4_3984 0.41 -0.1
4,426,015 + Pf1N1B4_3984 0.58 -0.1
4,426,150 + Pf1N1B4_3984 0.65 -0.1
4,426,151 - Pf1N1B4_3984 0.65 -4.5
4,426,151 - Pf1N1B4_3984 0.65 -4.2
4,426,151 - Pf1N1B4_3984 0.65 -0.1
4,426,151 - Pf1N1B4_3984 0.65 -5.3
4,426,151 - Pf1N1B4_3984 0.65 -0.1
4,426,152 + Pf1N1B4_3984 0.65 -6.3
4,426,153 - Pf1N1B4_3984 0.65 -0.1
4,426,153 - Pf1N1B4_3984 0.65 -0.1
4,426,213 + Pf1N1B4_3984 0.68 -0.1
4,426,214 - Pf1N1B4_3984 0.69 -0.1
4,426,639 + -5.8
4,426,640 - -0.1
4,426,640 - -0.1
4,427,321 + Pf1N1B4_3985 0.60 -0.1
4,427,361 + Pf1N1B4_3985 0.65 -0.1
4,427,361 + Pf1N1B4_3985 0.65 -4.3
4,427,361 + Pf1N1B4_3985 0.65 -2.0

Or see this region's nucleotide sequence