Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3935 and Pf1N1B4_3936 are separated by 3 nucleotides Pf1N1B4_3936 and Pf1N1B4_3937 are separated by 181 nucleotides Pf1N1B4_3937 and Pf1N1B4_3938 are separated by 28 nucleotides
Pf1N1B4_3935 - Major facilitator family transporter, at 4,372,211 to 4,373,365
_3935
Pf1N1B4_3936 - Transcriptional regulator, LysR family, at 4,373,369 to 4,374,268
_3936
Pf1N1B4_3937 - Membrane protein involved in colicin uptake, at 4,374,450 to 4,374,692
_3937
Pf1N1B4_3938 - Integral membrane protein TerC, at 4,374,721 to 4,375,470
_3938
Position (kb)
4373
4374
4375 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 4372.391 kb on + strand, within Pf1N1B4_3935 at 4372.581 kb on - strand, within Pf1N1B4_3935 at 4372.735 kb on + strand at 4372.735 kb on + strand at 4372.736 kb on - strand, within Pf1N1B4_3935 at 4372.740 kb on - strand at 4372.874 kb on + strand, within Pf1N1B4_3935 at 4372.874 kb on + strand, within Pf1N1B4_3935 at 4372.874 kb on + strand, within Pf1N1B4_3935 at 4372.875 kb on - strand, within Pf1N1B4_3935 at 4373.101 kb on + strand, within Pf1N1B4_3935 at 4373.457 kb on + strand at 4374.032 kb on + strand, within Pf1N1B4_3936 at 4374.033 kb on - strand, within Pf1N1B4_3936 at 4374.033 kb on - strand, within Pf1N1B4_3936 at 4374.138 kb on + strand, within Pf1N1B4_3936 at 4374.139 kb on - strand, within Pf1N1B4_3936 at 4374.139 kb on - strand, within Pf1N1B4_3936 at 4374.243 kb on + strand at 4374.244 kb on - strand at 4374.244 kb on - strand at 4374.314 kb on + strand at 4374.387 kb on + strand at 4374.388 kb on - strand at 4374.388 kb on - strand at 4374.458 kb on + strand at 4374.458 kb on + strand at 4374.458 kb on + strand at 4374.459 kb on - strand at 4374.459 kb on - strand at 4374.459 kb on - strand at 4374.690 kb on + strand at 4374.690 kb on + strand at 4374.699 kb on + strand at 4374.727 kb on + strand at 4374.734 kb on + strand at 4374.734 kb on + strand at 4374.734 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 4,372,391 + Pf1N1B4_3935 0.16 -0.1 4,372,581 - Pf1N1B4_3935 0.32 -3.1 4,372,735 + -0.1 4,372,735 + -0.1 4,372,736 - Pf1N1B4_3935 0.45 -2.7 4,372,740 - -0.1 4,372,874 + Pf1N1B4_3935 0.57 -0.1 4,372,874 + Pf1N1B4_3935 0.57 -2.1 4,372,874 + Pf1N1B4_3935 0.57 -1.0 4,372,875 - Pf1N1B4_3935 0.57 -0.1 4,373,101 + Pf1N1B4_3935 0.77 -0.1 4,373,457 + -2.2 4,374,032 + Pf1N1B4_3936 0.74 -0.1 4,374,033 - Pf1N1B4_3936 0.74 -1.1 4,374,033 - Pf1N1B4_3936 0.74 -4.2 4,374,138 + Pf1N1B4_3936 0.85 -0.1 4,374,139 - Pf1N1B4_3936 0.86 -0.1 4,374,139 - Pf1N1B4_3936 0.86 -0.1 4,374,243 + -0.1 4,374,244 - -0.1 4,374,244 - -2.0 4,374,314 + -2.0 4,374,387 + -0.1 4,374,388 - -3.8 4,374,388 - -5.3 4,374,458 + -0.1 4,374,458 + -5.1 4,374,458 + -0.1 4,374,459 - -5.7 4,374,459 - -0.1 4,374,459 - -0.1 4,374,690 + -2.3 4,374,690 + -0.1 4,374,699 + -1.3 4,374,727 + -0.1 4,374,734 + -0.1 4,374,734 + -0.1 4,374,734 + -0.1
Or see this region's nucleotide sequence