Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3842 and Pf1N1B4_3843 are separated by 302 nucleotides Pf1N1B4_3843 and Pf1N1B4_3844 are separated by 530 nucleotides
Pf1N1B4_3842 - Dihydroorotate dehydrogenase (EC 1.3.3.1), at 4,259,609 to 4,260,628
_3842
Pf1N1B4_3843 - Ribosome modulation factor, at 4,260,931 to 4,261,146
_3843
Pf1N1B4_3844 - 23S rRNA (guanine-N-2-) -methyltransferase rlmL EC 2.1.1.-), at 4,261,677 to 4,263,947
_3844
Position (kb)
4260
4261
4262 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 4260.612 kb on + strand at 4260.653 kb on + strand at 4260.654 kb on - strand at 4260.726 kb on + strand at 4260.726 kb on + strand at 4260.809 kb on + strand at 4260.809 kb on + strand at 4260.811 kb on + strand at 4260.811 kb on + strand at 4260.811 kb on + strand at 4260.811 kb on + strand at 4260.811 kb on + strand at 4260.811 kb on + strand at 4260.811 kb on + strand at 4260.811 kb on + strand at 4260.811 kb on + strand at 4260.812 kb on - strand at 4260.884 kb on + strand at 4260.885 kb on - strand at 4260.885 kb on - strand at 4260.930 kb on - strand at 4260.985 kb on - strand, within Pf1N1B4_3843 at 4261.053 kb on + strand, within Pf1N1B4_3843 at 4261.053 kb on + strand, within Pf1N1B4_3843 at 4261.054 kb on - strand, within Pf1N1B4_3843 at 4261.054 kb on - strand, within Pf1N1B4_3843 at 4261.054 kb on - strand, within Pf1N1B4_3843 at 4261.092 kb on - strand, within Pf1N1B4_3843 at 4261.134 kb on + strand at 4261.134 kb on + strand at 4261.134 kb on + strand at 4261.338 kb on + strand at 4261.365 kb on + strand at 4261.374 kb on - strand at 4261.374 kb on - strand at 4261.475 kb on + strand at 4261.538 kb on + strand at 4261.538 kb on + strand at 4261.538 kb on + strand at 4261.538 kb on + strand at 4261.539 kb on - strand at 4261.539 kb on - strand at 4261.539 kb on - strand at 4261.539 kb on - strand at 4261.539 kb on - strand at 4261.539 kb on - strand at 4261.548 kb on + strand at 4261.654 kb on + strand at 4261.654 kb on + strand at 4261.654 kb on + strand at 4261.673 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 4,260,612 + -0.1 4,260,653 + -0.1 4,260,654 - -3.1 4,260,726 + -0.6 4,260,726 + -0.1 4,260,809 + -0.1 4,260,809 + -0.1 4,260,811 + -0.1 4,260,811 + -3.0 4,260,811 + -3.8 4,260,811 + -0.1 4,260,811 + -0.1 4,260,811 + -0.1 4,260,811 + -5.9 4,260,811 + -0.1 4,260,811 + -2.0 4,260,812 - -0.1 4,260,884 + -0.1 4,260,885 - -0.1 4,260,885 - -0.1 4,260,930 - -5.0 4,260,985 - Pf1N1B4_3843 0.25 -3.5 4,261,053 + Pf1N1B4_3843 0.56 -1.1 4,261,053 + Pf1N1B4_3843 0.56 -0.1 4,261,054 - Pf1N1B4_3843 0.57 -0.1 4,261,054 - Pf1N1B4_3843 0.57 -0.1 4,261,054 - Pf1N1B4_3843 0.57 -0.1 4,261,092 - Pf1N1B4_3843 0.75 -0.1 4,261,134 + -6.3 4,261,134 + -0.7 4,261,134 + -0.1 4,261,338 + -6.1 4,261,365 + -0.1 4,261,374 - -0.1 4,261,374 - -0.1 4,261,475 + -1.8 4,261,538 + -0.1 4,261,538 + -1.2 4,261,538 + -0.1 4,261,538 + -0.1 4,261,539 - -3.9 4,261,539 - -0.1 4,261,539 - -0.1 4,261,539 - -0.1 4,261,539 - -0.1 4,261,539 - -1.8 4,261,548 + -0.1 4,261,654 + -1.6 4,261,654 + -1.5 4,261,654 + -2.2 4,261,673 + -0.1
Or see this region's nucleotide sequence