Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3791 and Pf1N1B4_3792 overlap by 4 nucleotides Pf1N1B4_3792 and Pf1N1B4_3793 are separated by 140 nucleotides Pf1N1B4_3793 and Pf1N1B4_3794 overlap by 2 nucleotides Pf1N1B4_3794 and Pf1N1B4_3795 are separated by 477 nucleotides
Pf1N1B4_3791 - Protein containing domains DUF403, at 4,199,431 to 4,200,381
_3791
Pf1N1B4_3792 - Protein containing transglutaminase-like domain, putative cysteine protease, at 4,200,378 to 4,201,187
_3792
Pf1N1B4_3793 - COG2010: Cytochrome c, mono- and diheme variants, at 4,201,328 to 4,202,149
_3793
Pf1N1B4_3794 - hypothetical protein, at 4,202,148 to 4,202,318
_3794
Pf1N1B4_3795 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 4,202,796 to 4,204,001
_3795
Position (kb)
4201
4202
4203 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 4200.353 kb on + strand at 4200.354 kb on - strand at 4200.408 kb on + strand at 4200.408 kb on + strand at 4200.408 kb on + strand at 4200.408 kb on + strand at 4200.408 kb on + strand at 4200.409 kb on - strand at 4200.409 kb on - strand at 4200.409 kb on - strand at 4200.409 kb on - strand at 4200.409 kb on - strand at 4200.409 kb on - strand at 4200.409 kb on - strand at 4200.445 kb on - strand at 4200.746 kb on + strand, within Pf1N1B4_3792 at 4200.792 kb on + strand, within Pf1N1B4_3792 at 4200.793 kb on - strand, within Pf1N1B4_3792 at 4200.793 kb on - strand, within Pf1N1B4_3792 at 4200.814 kb on + strand, within Pf1N1B4_3792 at 4200.814 kb on + strand, within Pf1N1B4_3792 at 4200.815 kb on - strand, within Pf1N1B4_3792 at 4200.815 kb on - strand, within Pf1N1B4_3792 at 4200.815 kb on - strand, within Pf1N1B4_3792 at 4200.815 kb on - strand, within Pf1N1B4_3792 at 4200.918 kb on + strand, within Pf1N1B4_3792 at 4200.930 kb on + strand, within Pf1N1B4_3792 at 4200.936 kb on + strand, within Pf1N1B4_3792 at 4200.936 kb on + strand, within Pf1N1B4_3792 at 4200.936 kb on + strand, within Pf1N1B4_3792 at 4200.936 kb on + strand, within Pf1N1B4_3792 at 4200.937 kb on - strand, within Pf1N1B4_3792 at 4200.937 kb on - strand, within Pf1N1B4_3792 at 4200.937 kb on - strand, within Pf1N1B4_3792 at 4200.937 kb on - strand, within Pf1N1B4_3792 at 4200.937 kb on - strand, within Pf1N1B4_3792 at 4200.937 kb on - strand, within Pf1N1B4_3792 at 4201.003 kb on - strand, within Pf1N1B4_3792 at 4201.072 kb on - strand at 4201.185 kb on + strand at 4201.190 kb on + strand at 4201.191 kb on - strand at 4201.191 kb on - strand at 4201.365 kb on + strand at 4201.366 kb on - strand at 4201.467 kb on + strand, within Pf1N1B4_3793 at 4201.467 kb on + strand, within Pf1N1B4_3793 at 4201.468 kb on - strand, within Pf1N1B4_3793 at 4201.468 kb on - strand, within Pf1N1B4_3793 at 4201.468 kb on - strand, within Pf1N1B4_3793 at 4201.494 kb on + strand, within Pf1N1B4_3793 at 4201.720 kb on + strand at 4201.720 kb on + strand at 4201.843 kb on + strand, within Pf1N1B4_3793 at 4201.956 kb on + strand, within Pf1N1B4_3793 at 4201.957 kb on - strand, within Pf1N1B4_3793 at 4202.216 kb on - strand, within Pf1N1B4_3794 at 4202.216 kb on - strand, within Pf1N1B4_3794 at 4202.290 kb on + strand, within Pf1N1B4_3794 at 4202.300 kb on + strand, within Pf1N1B4_3794 at 4202.300 kb on + strand, within Pf1N1B4_3794 at 4202.301 kb on - strand at 4202.301 kb on - strand, within Pf1N1B4_3794 at 4202.301 kb on - strand, within Pf1N1B4_3794 at 4202.416 kb on - strand at 4202.457 kb on - strand at 4202.464 kb on + strand at 4202.465 kb on - strand at 4202.465 kb on - strand at 4202.465 kb on - strand at 4202.492 kb on + strand at 4202.492 kb on + strand at 4202.514 kb on - strand at 4202.547 kb on - strand at 4202.576 kb on - strand at 4202.687 kb on + strand at 4202.687 kb on + strand at 4202.687 kb on + strand at 4202.687 kb on + strand at 4202.700 kb on + strand at 4202.700 kb on + strand at 4202.701 kb on - strand at 4202.812 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 4,200,353 + -1.7 4,200,354 - -0.1 4,200,408 + -0.1 4,200,408 + -0.1 4,200,408 + -0.1 4,200,408 + -0.1 4,200,408 + -1.1 4,200,409 - -0.1 4,200,409 - +1.1 4,200,409 - -0.1 4,200,409 - -2.0 4,200,409 - -0.1 4,200,409 - -0.1 4,200,409 - -3.0 4,200,445 - -1.4 4,200,746 + Pf1N1B4_3792 0.45 -2.2 4,200,792 + Pf1N1B4_3792 0.51 -0.1 4,200,793 - Pf1N1B4_3792 0.51 -0.1 4,200,793 - Pf1N1B4_3792 0.51 -0.1 4,200,814 + Pf1N1B4_3792 0.54 -0.1 4,200,814 + Pf1N1B4_3792 0.54 -0.2 4,200,815 - Pf1N1B4_3792 0.54 -0.1 4,200,815 - Pf1N1B4_3792 0.54 -0.1 4,200,815 - Pf1N1B4_3792 0.54 -0.1 4,200,815 - Pf1N1B4_3792 0.54 -0.1 4,200,918 + Pf1N1B4_3792 0.67 -1.1 4,200,930 + Pf1N1B4_3792 0.68 -0.1 4,200,936 + Pf1N1B4_3792 0.69 -0.1 4,200,936 + Pf1N1B4_3792 0.69 -0.1 4,200,936 + Pf1N1B4_3792 0.69 -0.1 4,200,936 + Pf1N1B4_3792 0.69 -0.8 4,200,937 - Pf1N1B4_3792 0.69 -1.0 4,200,937 - Pf1N1B4_3792 0.69 -2.5 4,200,937 - Pf1N1B4_3792 0.69 -0.1 4,200,937 - Pf1N1B4_3792 0.69 -4.4 4,200,937 - Pf1N1B4_3792 0.69 -0.1 4,200,937 - Pf1N1B4_3792 0.69 -0.1 4,201,003 - Pf1N1B4_3792 0.77 -0.5 4,201,072 - -0.1 4,201,185 + -1.2 4,201,190 + -2.4 4,201,191 - -0.1 4,201,191 - -1.3 4,201,365 + -3.4 4,201,366 - -0.1 4,201,467 + Pf1N1B4_3793 0.17 -0.1 4,201,467 + Pf1N1B4_3793 0.17 -0.1 4,201,468 - Pf1N1B4_3793 0.17 -0.1 4,201,468 - Pf1N1B4_3793 0.17 -5.1 4,201,468 - Pf1N1B4_3793 0.17 -0.1 4,201,494 + Pf1N1B4_3793 0.20 -3.3 4,201,720 + -4.7 4,201,720 + -0.1 4,201,843 + Pf1N1B4_3793 0.63 -0.1 4,201,956 + Pf1N1B4_3793 0.76 -0.1 4,201,957 - Pf1N1B4_3793 0.77 -0.1 4,202,216 - Pf1N1B4_3794 0.40 -4.2 4,202,216 - Pf1N1B4_3794 0.40 -2.0 4,202,290 + Pf1N1B4_3794 0.83 -0.1 4,202,300 + Pf1N1B4_3794 0.89 +0.1 4,202,300 + Pf1N1B4_3794 0.89 -0.1 4,202,301 - -0.1 4,202,301 - Pf1N1B4_3794 0.89 -0.1 4,202,301 - Pf1N1B4_3794 0.89 -2.5 4,202,416 - -0.1 4,202,457 - -2.9 4,202,464 + -0.1 4,202,465 - -3.2 4,202,465 - +1.3 4,202,465 - -0.1 4,202,492 + -0.1 4,202,492 + -1.4 4,202,514 - -4.5 4,202,547 - -1.2 4,202,576 - -1.0 4,202,687 + -0.8 4,202,687 + -0.1 4,202,687 + -0.1 4,202,687 + -2.6 4,202,700 + -0.1 4,202,700 + -0.1 4,202,701 - -0.1 4,202,812 + -1.6
Or see this region's nucleotide sequence