Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_371 and Pf1N1B4_372 are separated by 105 nucleotides Pf1N1B4_372 and Pf1N1B4_373 are separated by 323 nucleotides
Pf1N1B4_371 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16), at 438,340 to 439,572
_371
Pf1N1B4_372 - UDP-galactose-lipid carrier transferase (EC 2.-.-.-), at 439,678 to 441,201
_372
Pf1N1B4_373 - 5-methylaminomethyl-2-thiouridine-forming enzyme mnmC, at 441,525 to 443,507
_373
Position (kb) 439
440
441
442 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 438.983 kb on + strand at 438.983 kb on + strand, within Pf1N1B4_371 at 438.984 kb on - strand at 439.068 kb on - strand, within Pf1N1B4_371 at 439.068 kb on - strand, within Pf1N1B4_371 at 439.068 kb on - strand, within Pf1N1B4_371 at 439.139 kb on + strand, within Pf1N1B4_371 at 439.139 kb on + strand, within Pf1N1B4_371 at 439.139 kb on + strand, within Pf1N1B4_371 at 439.139 kb on + strand, within Pf1N1B4_371 at 439.139 kb on + strand, within Pf1N1B4_371 at 439.139 kb on + strand, within Pf1N1B4_371 at 439.139 kb on + strand at 439.139 kb on + strand, within Pf1N1B4_371 at 439.140 kb on - strand, within Pf1N1B4_371 at 439.140 kb on - strand, within Pf1N1B4_371 at 439.140 kb on - strand, within Pf1N1B4_371 at 439.140 kb on - strand, within Pf1N1B4_371 at 439.140 kb on - strand, within Pf1N1B4_371 at 439.140 kb on - strand, within Pf1N1B4_371 at 439.577 kb on + strand at 439.577 kb on + strand at 439.577 kb on + strand at 439.577 kb on + strand at 439.578 kb on - strand at 439.578 kb on - strand at 439.578 kb on - strand at 439.578 kb on - strand at 439.618 kb on + strand at 439.619 kb on - strand at 439.619 kb on - strand at 439.619 kb on - strand at 439.619 kb on - strand at 439.629 kb on + strand at 439.630 kb on - strand at 439.816 kb on + strand at 439.817 kb on - strand at 440.171 kb on + strand, within Pf1N1B4_372 at 440.223 kb on - strand, within Pf1N1B4_372 at 440.381 kb on + strand, within Pf1N1B4_372 at 440.382 kb on - strand, within Pf1N1B4_372 at 440.547 kb on + strand, within Pf1N1B4_372 at 440.549 kb on + strand, within Pf1N1B4_372 at 440.550 kb on - strand, within Pf1N1B4_372 at 440.624 kb on + strand, within Pf1N1B4_372 at 440.624 kb on + strand at 440.625 kb on - strand, within Pf1N1B4_372 at 440.643 kb on - strand, within Pf1N1B4_372 at 441.155 kb on + strand at 441.155 kb on + strand at 441.155 kb on + strand at 441.156 kb on - strand at 441.156 kb on - strand at 441.156 kb on - strand at 441.201 kb on + strand at 441.201 kb on + strand at 441.202 kb on - strand at 441.250 kb on + strand at 441.250 kb on + strand at 441.250 kb on + strand at 441.250 kb on + strand at 441.250 kb on + strand at 441.250 kb on + strand at 441.250 kb on + strand at 441.250 kb on + strand at 441.250 kb on + strand at 441.251 kb on - strand at 441.251 kb on - strand at 441.251 kb on - strand at 441.251 kb on - strand at 441.251 kb on - strand at 441.254 kb on + strand at 441.254 kb on + strand at 441.254 kb on + strand at 441.255 kb on - strand at 441.255 kb on - strand at 441.255 kb on - strand at 441.255 kb on - strand at 441.360 kb on + strand at 441.360 kb on + strand at 441.361 kb on - strand at 441.361 kb on - strand at 441.361 kb on - strand at 441.361 kb on - strand at 441.364 kb on + strand at 441.364 kb on + strand at 441.364 kb on + strand at 441.364 kb on + strand at 441.364 kb on + strand at 441.364 kb on + strand at 441.365 kb on - strand at 441.365 kb on - strand at 441.365 kb on - strand at 441.365 kb on - strand at 441.365 kb on - strand at 441.365 kb on - strand at 441.603 kb on + strand at 441.603 kb on + strand at 441.603 kb on + strand at 441.603 kb on + strand at 441.603 kb on + strand at 441.900 kb on + strand, within Pf1N1B4_373
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 438,983 + -0.2 438,983 + Pf1N1B4_371 0.52 -0.1 438,984 - -0.1 439,068 - Pf1N1B4_371 0.59 -0.1 439,068 - Pf1N1B4_371 0.59 -0.1 439,068 - Pf1N1B4_371 0.59 -0.1 439,139 + Pf1N1B4_371 0.65 -0.1 439,139 + Pf1N1B4_371 0.65 -0.1 439,139 + Pf1N1B4_371 0.65 -4.5 439,139 + Pf1N1B4_371 0.65 -0.1 439,139 + Pf1N1B4_371 0.65 -2.2 439,139 + Pf1N1B4_371 0.65 -1.8 439,139 + -1.7 439,139 + Pf1N1B4_371 0.65 -0.1 439,140 - Pf1N1B4_371 0.65 -0.1 439,140 - Pf1N1B4_371 0.65 -0.1 439,140 - Pf1N1B4_371 0.65 -0.1 439,140 - Pf1N1B4_371 0.65 -0.1 439,140 - Pf1N1B4_371 0.65 -2.1 439,140 - Pf1N1B4_371 0.65 -0.1 439,577 + -0.1 439,577 + -0.1 439,577 + -0.1 439,577 + -1.7 439,578 - -0.1 439,578 - -0.1 439,578 - -0.1 439,578 - -0.1 439,618 + +0.0 439,619 - -0.1 439,619 - -0.1 439,619 - -0.1 439,619 - -0.1 439,629 + -0.1 439,630 - -2.0 439,816 + -0.1 439,817 - +0.0 440,171 + Pf1N1B4_372 0.32 -0.1 440,223 - Pf1N1B4_372 0.36 -3.2 440,381 + Pf1N1B4_372 0.46 -0.1 440,382 - Pf1N1B4_372 0.46 -0.1 440,547 + Pf1N1B4_372 0.57 -0.1 440,549 + Pf1N1B4_372 0.57 -0.1 440,550 - Pf1N1B4_372 0.57 -2.6 440,624 + Pf1N1B4_372 0.62 -0.1 440,624 + -0.1 440,625 - Pf1N1B4_372 0.62 -0.1 440,643 - Pf1N1B4_372 0.63 -0.1 441,155 + -0.5 441,155 + -0.1 441,155 + -0.1 441,156 - -0.1 441,156 - -0.1 441,156 - -0.1 441,201 + -1.7 441,201 + -1.6 441,202 - -1.0 441,250 + -0.1 441,250 + +0.2 441,250 + -1.2 441,250 + -0.1 441,250 + -2.7 441,250 + -0.1 441,250 + -0.1 441,250 + -0.1 441,250 + -0.1 441,251 - -0.1 441,251 - -0.1 441,251 - -0.1 441,251 - -0.1 441,251 - -0.1 441,254 + -0.1 441,254 + -0.1 441,254 + -1.4 441,255 - -0.1 441,255 - -0.1 441,255 - -0.1 441,255 - -1.0 441,360 + -0.1 441,360 + -0.1 441,361 - -0.1 441,361 - -0.1 441,361 - -0.1 441,361 - -0.4 441,364 + -0.1 441,364 + -0.1 441,364 + -0.1 441,364 + -2.3 441,364 + -4.0 441,364 + -0.1 441,365 - -0.1 441,365 - -0.1 441,365 - -0.1 441,365 - -0.1 441,365 - -0.1 441,365 - -1.7 441,603 + -0.1 441,603 + -0.1 441,603 + -3.9 441,603 + -1.5 441,603 + -4.6 441,900 + Pf1N1B4_373 0.19 -0.1
Or see this region's nucleotide sequence