Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3717 and Pf1N1B4_3718 overlap by 4 nucleotides Pf1N1B4_3718 and Pf1N1B4_3719 are separated by 56 nucleotides Pf1N1B4_3719 and Pf1N1B4_3720 are separated by 83 nucleotides
Pf1N1B4_3717 - Permeases of the major facilitator superfamily, at 4,124,721 to 4,125,929
_3717
Pf1N1B4_3718 - hypothetical protein, at 4,125,926 to 4,126,048
_3718
Pf1N1B4_3719 - Glutathione peroxidase family protein, at 4,126,105 to 4,126,656
_3719
Pf1N1B4_3720 - Long-chain fatty acid transport protein, at 4,126,740 to 4,128,008
_3720
Position (kb)
4126
4127 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 4125.429 kb on + strand, within Pf1N1B4_3717 at 4125.567 kb on + strand, within Pf1N1B4_3717 at 4125.567 kb on + strand, within Pf1N1B4_3717 at 4125.567 kb on + strand, within Pf1N1B4_3717 at 4125.567 kb on + strand, within Pf1N1B4_3717 at 4125.568 kb on - strand, within Pf1N1B4_3717 at 4125.630 kb on + strand, within Pf1N1B4_3717 at 4125.631 kb on - strand, within Pf1N1B4_3717 at 4125.798 kb on + strand, within Pf1N1B4_3717 at 4125.798 kb on + strand, within Pf1N1B4_3717 at 4125.798 kb on + strand, within Pf1N1B4_3717 at 4125.798 kb on + strand at 4125.798 kb on + strand, within Pf1N1B4_3717 at 4125.799 kb on - strand at 4125.799 kb on - strand, within Pf1N1B4_3717 at 4125.816 kb on + strand at 4125.817 kb on - strand at 4125.969 kb on + strand, within Pf1N1B4_3718 at 4125.970 kb on - strand, within Pf1N1B4_3718 at 4126.066 kb on + strand at 4126.187 kb on + strand, within Pf1N1B4_3719 at 4126.187 kb on + strand, within Pf1N1B4_3719 at 4126.187 kb on + strand, within Pf1N1B4_3719 at 4126.188 kb on - strand, within Pf1N1B4_3719 at 4126.188 kb on - strand, within Pf1N1B4_3719 at 4126.324 kb on + strand, within Pf1N1B4_3719 at 4126.333 kb on + strand, within Pf1N1B4_3719 at 4126.423 kb on + strand, within Pf1N1B4_3719 at 4126.424 kb on - strand, within Pf1N1B4_3719 at 4126.432 kb on + strand, within Pf1N1B4_3719 at 4126.432 kb on + strand, within Pf1N1B4_3719 at 4126.540 kb on + strand, within Pf1N1B4_3719 at 4126.540 kb on + strand, within Pf1N1B4_3719 at 4126.546 kb on + strand, within Pf1N1B4_3719 at 4126.546 kb on + strand, within Pf1N1B4_3719 at 4126.546 kb on + strand, within Pf1N1B4_3719 at 4126.546 kb on + strand, within Pf1N1B4_3719 at 4126.546 kb on + strand at 4126.668 kb on + strand at 4126.687 kb on - strand at 4126.750 kb on + strand at 4126.750 kb on + strand at 4126.750 kb on + strand at 4126.750 kb on + strand at 4126.750 kb on + strand at 4126.751 kb on - strand at 4126.780 kb on + strand at 4126.780 kb on + strand at 4126.780 kb on + strand at 4126.780 kb on + strand at 4126.781 kb on - strand at 4126.781 kb on - strand at 4126.781 kb on - strand at 4126.781 kb on - strand at 4126.781 kb on - strand at 4126.789 kb on + strand at 4126.790 kb on - strand at 4126.801 kb on + strand at 4126.801 kb on + strand at 4126.802 kb on - strand at 4126.802 kb on - strand at 4126.802 kb on - strand at 4126.802 kb on - strand at 4126.802 kb on - strand at 4126.802 kb on - strand at 4126.852 kb on + strand at 4126.852 kb on + strand at 4126.852 kb on + strand at 4126.852 kb on + strand at 4126.852 kb on + strand at 4126.853 kb on - strand at 4126.853 kb on - strand at 4126.858 kb on + strand at 4126.858 kb on + strand at 4126.858 kb on + strand at 4126.858 kb on + strand at 4126.859 kb on - strand at 4126.859 kb on - strand at 4126.859 kb on - strand at 4126.863 kb on + strand at 4126.863 kb on + strand at 4126.863 kb on + strand at 4126.864 kb on - strand at 4126.864 kb on - strand at 4126.864 kb on - strand at 4126.864 kb on - strand at 4126.864 kb on - strand at 4126.864 kb on - strand at 4127.162 kb on + strand at 4127.163 kb on - strand at 4127.163 kb on - strand, within Pf1N1B4_3720 at 4127.170 kb on + strand, within Pf1N1B4_3720 at 4127.170 kb on + strand, within Pf1N1B4_3720 at 4127.251 kb on + strand, within Pf1N1B4_3720 at 4127.251 kb on + strand, within Pf1N1B4_3720 at 4127.251 kb on + strand, within Pf1N1B4_3720 at 4127.251 kb on + strand, within Pf1N1B4_3720 at 4127.252 kb on - strand, within Pf1N1B4_3720 at 4127.252 kb on - strand, within Pf1N1B4_3720 at 4127.252 kb on - strand, within Pf1N1B4_3720 at 4127.252 kb on - strand, within Pf1N1B4_3720 at 4127.293 kb on + strand, within Pf1N1B4_3720 at 4127.293 kb on + strand, within Pf1N1B4_3720 at 4127.294 kb on - strand, within Pf1N1B4_3720 at 4127.294 kb on - strand, within Pf1N1B4_3720 at 4127.294 kb on - strand at 4127.323 kb on + strand at 4127.323 kb on + strand, within Pf1N1B4_3720 at 4127.323 kb on + strand, within Pf1N1B4_3720 at 4127.324 kb on - strand, within Pf1N1B4_3720 at 4127.324 kb on - strand, within Pf1N1B4_3720 at 4127.324 kb on - strand, within Pf1N1B4_3720 at 4127.324 kb on - strand, within Pf1N1B4_3720 at 4127.324 kb on - strand, within Pf1N1B4_3720 at 4127.324 kb on - strand, within Pf1N1B4_3720 at 4127.341 kb on + strand, within Pf1N1B4_3720 at 4127.341 kb on + strand, within Pf1N1B4_3720 at 4127.341 kb on + strand at 4127.396 kb on - strand, within Pf1N1B4_3720 at 4127.536 kb on + strand, within Pf1N1B4_3720 at 4127.536 kb on + strand, within Pf1N1B4_3720 at 4127.537 kb on - strand, within Pf1N1B4_3720 at 4127.537 kb on - strand at 4127.537 kb on - strand, within Pf1N1B4_3720 at 4127.590 kb on + strand at 4127.590 kb on + strand, within Pf1N1B4_3720 at 4127.591 kb on - strand, within Pf1N1B4_3720 at 4127.591 kb on - strand, within Pf1N1B4_3720 at 4127.591 kb on - strand at 4127.591 kb on - strand at 4127.615 kb on - strand at 4127.615 kb on - strand at 4127.615 kb on - strand, within Pf1N1B4_3720 at 4127.642 kb on - strand, within Pf1N1B4_3720
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 4,125,429 + Pf1N1B4_3717 0.59 -0.1 4,125,567 + Pf1N1B4_3717 0.70 -0.1 4,125,567 + Pf1N1B4_3717 0.70 -0.1 4,125,567 + Pf1N1B4_3717 0.70 -0.5 4,125,567 + Pf1N1B4_3717 0.70 -0.1 4,125,568 - Pf1N1B4_3717 0.70 -0.1 4,125,630 + Pf1N1B4_3717 0.75 -0.1 4,125,631 - Pf1N1B4_3717 0.75 -0.2 4,125,798 + Pf1N1B4_3717 0.89 -0.1 4,125,798 + Pf1N1B4_3717 0.89 -0.1 4,125,798 + Pf1N1B4_3717 0.89 -2.4 4,125,798 + -0.1 4,125,798 + Pf1N1B4_3717 0.89 -0.1 4,125,799 - -0.9 4,125,799 - Pf1N1B4_3717 0.89 -0.1 4,125,816 + -0.1 4,125,817 - -0.1 4,125,969 + Pf1N1B4_3718 0.35 -0.1 4,125,970 - Pf1N1B4_3718 0.36 -0.1 4,126,066 + -0.1 4,126,187 + Pf1N1B4_3719 0.15 -0.1 4,126,187 + Pf1N1B4_3719 0.15 -0.1 4,126,187 + Pf1N1B4_3719 0.15 -0.1 4,126,188 - Pf1N1B4_3719 0.15 -0.1 4,126,188 - Pf1N1B4_3719 0.15 -0.1 4,126,324 + Pf1N1B4_3719 0.40 -0.1 4,126,333 + Pf1N1B4_3719 0.41 -2.9 4,126,423 + Pf1N1B4_3719 0.58 -0.1 4,126,424 - Pf1N1B4_3719 0.58 -4.0 4,126,432 + Pf1N1B4_3719 0.59 -0.1 4,126,432 + Pf1N1B4_3719 0.59 -0.1 4,126,540 + Pf1N1B4_3719 0.79 -0.1 4,126,540 + Pf1N1B4_3719 0.79 -0.1 4,126,546 + Pf1N1B4_3719 0.80 -0.1 4,126,546 + Pf1N1B4_3719 0.80 -4.0 4,126,546 + Pf1N1B4_3719 0.80 -4.0 4,126,546 + Pf1N1B4_3719 0.80 -0.1 4,126,546 + -1.2 4,126,668 + -0.2 4,126,687 - -0.1 4,126,750 + -0.4 4,126,750 + -0.1 4,126,750 + -0.1 4,126,750 + -0.1 4,126,750 + -0.1 4,126,751 - -0.3 4,126,780 + -0.1 4,126,780 + -0.1 4,126,780 + -1.4 4,126,780 + -0.1 4,126,781 - -0.1 4,126,781 - -0.1 4,126,781 - -0.1 4,126,781 - -2.4 4,126,781 - -2.5 4,126,789 + -0.3 4,126,790 - -2.1 4,126,801 + -0.1 4,126,801 + -0.1 4,126,802 - -0.6 4,126,802 - -0.1 4,126,802 - -1.4 4,126,802 - -1.9 4,126,802 - -0.1 4,126,802 - -0.1 4,126,852 + -1.4 4,126,852 + -0.1 4,126,852 + -0.1 4,126,852 + -0.1 4,126,852 + -0.1 4,126,853 - -0.3 4,126,853 - -0.1 4,126,858 + -0.1 4,126,858 + -0.1 4,126,858 + -0.1 4,126,858 + -0.1 4,126,859 - -0.1 4,126,859 - -0.1 4,126,859 - -0.1 4,126,863 + -2.1 4,126,863 + -0.1 4,126,863 + -0.1 4,126,864 - -0.1 4,126,864 - -0.5 4,126,864 - -2.0 4,126,864 - -0.1 4,126,864 - -0.1 4,126,864 - -0.1 4,127,162 + -0.1 4,127,163 - -2.0 4,127,163 - Pf1N1B4_3720 0.33 -2.1 4,127,170 + Pf1N1B4_3720 0.34 -0.1 4,127,170 + Pf1N1B4_3720 0.34 -0.1 4,127,251 + Pf1N1B4_3720 0.40 -0.1 4,127,251 + Pf1N1B4_3720 0.40 +0.6 4,127,251 + Pf1N1B4_3720 0.40 -1.9 4,127,251 + Pf1N1B4_3720 0.40 -1.2 4,127,252 - Pf1N1B4_3720 0.40 -0.1 4,127,252 - Pf1N1B4_3720 0.40 -0.1 4,127,252 - Pf1N1B4_3720 0.40 -0.1 4,127,252 - Pf1N1B4_3720 0.40 -0.1 4,127,293 + Pf1N1B4_3720 0.44 -1.3 4,127,293 + Pf1N1B4_3720 0.44 -1.7 4,127,294 - Pf1N1B4_3720 0.44 -0.1 4,127,294 - Pf1N1B4_3720 0.44 -2.4 4,127,294 - -0.1 4,127,323 + -0.1 4,127,323 + Pf1N1B4_3720 0.46 -1.0 4,127,323 + Pf1N1B4_3720 0.46 +1.2 4,127,324 - Pf1N1B4_3720 0.46 -2.2 4,127,324 - Pf1N1B4_3720 0.46 -0.1 4,127,324 - Pf1N1B4_3720 0.46 -0.7 4,127,324 - Pf1N1B4_3720 0.46 -0.1 4,127,324 - Pf1N1B4_3720 0.46 -0.1 4,127,324 - Pf1N1B4_3720 0.46 -0.1 4,127,341 + Pf1N1B4_3720 0.47 -0.1 4,127,341 + Pf1N1B4_3720 0.47 -0.1 4,127,341 + -1.9 4,127,396 - Pf1N1B4_3720 0.52 -0.1 4,127,536 + Pf1N1B4_3720 0.63 -0.1 4,127,536 + Pf1N1B4_3720 0.63 -0.1 4,127,537 - Pf1N1B4_3720 0.63 -0.1 4,127,537 - -0.1 4,127,537 - Pf1N1B4_3720 0.63 -5.5 4,127,590 + -0.8 4,127,590 + Pf1N1B4_3720 0.67 -2.4 4,127,591 - Pf1N1B4_3720 0.67 -1.2 4,127,591 - Pf1N1B4_3720 0.67 -0.1 4,127,591 - -0.1 4,127,591 - -1.7 4,127,615 - -0.1 4,127,615 - -0.5 4,127,615 - Pf1N1B4_3720 0.69 -0.4 4,127,642 - Pf1N1B4_3720 0.71 -0.1
Or see this region's nucleotide sequence