Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3585

Experiment: Nitrate reduction

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3584 and Pf1N1B4_3585 are separated by 30 nucleotidesPf1N1B4_3585 and Pf1N1B4_3586 overlap by 4 nucleotides Pf1N1B4_3584 - O-antigen biosynthesis protein, at 3,994,479 to 3,997,415 _3584 Pf1N1B4_3585 - UDP-N-acetylglucosamine 4,6-dehydratase (EC 4.2.1.-), at 3,997,446 to 3,998,447 _3585 Pf1N1B4_3586 - DegT/DnrJ/EryC1/StrS aminotransferase, at 3,998,444 to 3,999,607 _3586 Position (kb) 3997 3998 3999Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 3996.843 kb on + strand, within Pf1N1B4_3584at 3996.844 kb on - strand, within Pf1N1B4_3584at 3997.051 kb on - strand, within Pf1N1B4_3584at 3997.170 kb on + strandat 3997.332 kb on + strandat 3997.367 kb on + strandat 3997.414 kb on - strandat 3997.431 kb on - strandat 3997.431 kb on - strandat 3997.575 kb on + strandat 3997.615 kb on - strand, within Pf1N1B4_3585at 3997.719 kb on + strand, within Pf1N1B4_3585at 3997.719 kb on + strand, within Pf1N1B4_3585at 3997.720 kb on - strand, within Pf1N1B4_3585at 3998.274 kb on + strand, within Pf1N1B4_3585at 3998.303 kb on + strand, within Pf1N1B4_3585at 3998.303 kb on + strand, within Pf1N1B4_3585at 3998.768 kb on + strand, within Pf1N1B4_3586at 3998.860 kb on + strand, within Pf1N1B4_3586at 3998.860 kb on + strand, within Pf1N1B4_3586at 3998.900 kb on - strand, within Pf1N1B4_3586at 3998.939 kb on + strand, within Pf1N1B4_3586at 3998.939 kb on + strand, within Pf1N1B4_3586at 3998.939 kb on + strand, within Pf1N1B4_3586at 3998.940 kb on - strand, within Pf1N1B4_3586at 3998.940 kb on - strand, within Pf1N1B4_3586at 3999.140 kb on + strand, within Pf1N1B4_3586at 3999.144 kb on - strand, within Pf1N1B4_3586at 3999.167 kb on - strand, within Pf1N1B4_3586at 3999.281 kb on + strand, within Pf1N1B4_3586at 3999.281 kb on + strand, within Pf1N1B4_3586at 3999.338 kb on + strand, within Pf1N1B4_3586at 3999.338 kb on + strandat 3999.338 kb on + strand, within Pf1N1B4_3586at 3999.339 kb on - strand, within Pf1N1B4_3586at 3999.339 kb on - strand, within Pf1N1B4_3586at 3999.339 kb on - strand, within Pf1N1B4_3586at 3999.431 kb on + strandat 3999.431 kb on + strand, within Pf1N1B4_3586at 3999.431 kb on + strand, within Pf1N1B4_3586at 3999.432 kb on - strand, within Pf1N1B4_3586at 3999.432 kb on - strand, within Pf1N1B4_3586at 3999.432 kb on - strand, within Pf1N1B4_3586at 3999.433 kb on + strand, within Pf1N1B4_3586at 3999.434 kb on - strand, within Pf1N1B4_3586at 3999.434 kb on - strand, within Pf1N1B4_3586at 3999.434 kb on - strand, within Pf1N1B4_3586at 3999.434 kb on - strand, within Pf1N1B4_3586at 3999.434 kb on - strand, within Pf1N1B4_3586at 3999.434 kb on - strand, within Pf1N1B4_3586at 3999.434 kb on - strand, within Pf1N1B4_3586

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction
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3,996,843 + Pf1N1B4_3584 0.80 -0.1
3,996,844 - Pf1N1B4_3584 0.81 -0.1
3,997,051 - Pf1N1B4_3584 0.88 -0.1
3,997,170 + -4.5
3,997,332 + -0.1
3,997,367 + -0.9
3,997,414 - -0.1
3,997,431 - -0.1
3,997,431 - -0.1
3,997,575 + -2.2
3,997,615 - Pf1N1B4_3585 0.17 -0.1
3,997,719 + Pf1N1B4_3585 0.27 -2.2
3,997,719 + Pf1N1B4_3585 0.27 -2.5
3,997,720 - Pf1N1B4_3585 0.27 -0.1
3,998,274 + Pf1N1B4_3585 0.83 -1.1
3,998,303 + Pf1N1B4_3585 0.86 -3.0
3,998,303 + Pf1N1B4_3585 0.86 -4.0
3,998,768 + Pf1N1B4_3586 0.28 -1.1
3,998,860 + Pf1N1B4_3586 0.36 -0.1
3,998,860 + Pf1N1B4_3586 0.36 -4.4
3,998,900 - Pf1N1B4_3586 0.39 -1.9
3,998,939 + Pf1N1B4_3586 0.43 -0.1
3,998,939 + Pf1N1B4_3586 0.43 -0.1
3,998,939 + Pf1N1B4_3586 0.43 -0.1
3,998,940 - Pf1N1B4_3586 0.43 -0.9
3,998,940 - Pf1N1B4_3586 0.43 -1.5
3,999,140 + Pf1N1B4_3586 0.60 -0.1
3,999,144 - Pf1N1B4_3586 0.60 -0.1
3,999,167 - Pf1N1B4_3586 0.62 -0.1
3,999,281 + Pf1N1B4_3586 0.72 +0.0
3,999,281 + Pf1N1B4_3586 0.72 -1.7
3,999,338 + Pf1N1B4_3586 0.77 -0.6
3,999,338 + -2.4
3,999,338 + Pf1N1B4_3586 0.77 -6.0
3,999,339 - Pf1N1B4_3586 0.77 -1.4
3,999,339 - Pf1N1B4_3586 0.77 -0.1
3,999,339 - Pf1N1B4_3586 0.77 +0.7
3,999,431 + -1.6
3,999,431 + Pf1N1B4_3586 0.85 -0.1
3,999,431 + Pf1N1B4_3586 0.85 -0.1
3,999,432 - Pf1N1B4_3586 0.85 -0.1
3,999,432 - Pf1N1B4_3586 0.85 -4.6
3,999,432 - Pf1N1B4_3586 0.85 -0.1
3,999,433 + Pf1N1B4_3586 0.85 -0.1
3,999,434 - Pf1N1B4_3586 0.85 -0.1
3,999,434 - Pf1N1B4_3586 0.85 -0.1
3,999,434 - Pf1N1B4_3586 0.85 -0.1
3,999,434 - Pf1N1B4_3586 0.85 -3.0
3,999,434 - Pf1N1B4_3586 0.85 -1.9
3,999,434 - Pf1N1B4_3586 0.85 -0.1
3,999,434 - Pf1N1B4_3586 0.85 -0.1

Or see this region's nucleotide sequence