Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3584 and Pf1N1B4_3585 are separated by 30 nucleotides Pf1N1B4_3585 and Pf1N1B4_3586 overlap by 4 nucleotides
Pf1N1B4_3584 - O-antigen biosynthesis protein, at 3,994,479 to 3,997,415
_3584
Pf1N1B4_3585 - UDP-N-acetylglucosamine 4,6-dehydratase (EC 4.2.1.-), at 3,997,446 to 3,998,447
_3585
Pf1N1B4_3586 - DegT/DnrJ/EryC1/StrS aminotransferase, at 3,998,444 to 3,999,607
_3586
Position (kb)
3997
3998
3999 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 3996.843 kb on + strand, within Pf1N1B4_3584 at 3996.844 kb on - strand, within Pf1N1B4_3584 at 3997.051 kb on - strand, within Pf1N1B4_3584 at 3997.170 kb on + strand at 3997.332 kb on + strand at 3997.367 kb on + strand at 3997.414 kb on - strand at 3997.431 kb on - strand at 3997.431 kb on - strand at 3997.575 kb on + strand at 3997.615 kb on - strand, within Pf1N1B4_3585 at 3997.719 kb on + strand, within Pf1N1B4_3585 at 3997.719 kb on + strand, within Pf1N1B4_3585 at 3997.720 kb on - strand, within Pf1N1B4_3585 at 3998.274 kb on + strand, within Pf1N1B4_3585 at 3998.303 kb on + strand, within Pf1N1B4_3585 at 3998.303 kb on + strand, within Pf1N1B4_3585 at 3998.768 kb on + strand, within Pf1N1B4_3586 at 3998.860 kb on + strand, within Pf1N1B4_3586 at 3998.860 kb on + strand, within Pf1N1B4_3586 at 3998.900 kb on - strand, within Pf1N1B4_3586 at 3998.939 kb on + strand, within Pf1N1B4_3586 at 3998.939 kb on + strand, within Pf1N1B4_3586 at 3998.939 kb on + strand, within Pf1N1B4_3586 at 3998.940 kb on - strand, within Pf1N1B4_3586 at 3998.940 kb on - strand, within Pf1N1B4_3586 at 3999.140 kb on + strand, within Pf1N1B4_3586 at 3999.144 kb on - strand, within Pf1N1B4_3586 at 3999.167 kb on - strand, within Pf1N1B4_3586 at 3999.281 kb on + strand, within Pf1N1B4_3586 at 3999.281 kb on + strand, within Pf1N1B4_3586 at 3999.338 kb on + strand, within Pf1N1B4_3586 at 3999.338 kb on + strand at 3999.338 kb on + strand, within Pf1N1B4_3586 at 3999.339 kb on - strand, within Pf1N1B4_3586 at 3999.339 kb on - strand, within Pf1N1B4_3586 at 3999.339 kb on - strand, within Pf1N1B4_3586 at 3999.431 kb on + strand at 3999.431 kb on + strand, within Pf1N1B4_3586 at 3999.431 kb on + strand, within Pf1N1B4_3586 at 3999.432 kb on - strand, within Pf1N1B4_3586 at 3999.432 kb on - strand, within Pf1N1B4_3586 at 3999.432 kb on - strand, within Pf1N1B4_3586 at 3999.433 kb on + strand, within Pf1N1B4_3586 at 3999.434 kb on - strand, within Pf1N1B4_3586 at 3999.434 kb on - strand, within Pf1N1B4_3586 at 3999.434 kb on - strand, within Pf1N1B4_3586 at 3999.434 kb on - strand, within Pf1N1B4_3586 at 3999.434 kb on - strand, within Pf1N1B4_3586 at 3999.434 kb on - strand, within Pf1N1B4_3586 at 3999.434 kb on - strand, within Pf1N1B4_3586
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 3,996,843 + Pf1N1B4_3584 0.80 -0.1 3,996,844 - Pf1N1B4_3584 0.81 -0.1 3,997,051 - Pf1N1B4_3584 0.88 -0.1 3,997,170 + -4.5 3,997,332 + -0.1 3,997,367 + -0.9 3,997,414 - -0.1 3,997,431 - -0.1 3,997,431 - -0.1 3,997,575 + -2.2 3,997,615 - Pf1N1B4_3585 0.17 -0.1 3,997,719 + Pf1N1B4_3585 0.27 -2.2 3,997,719 + Pf1N1B4_3585 0.27 -2.5 3,997,720 - Pf1N1B4_3585 0.27 -0.1 3,998,274 + Pf1N1B4_3585 0.83 -1.1 3,998,303 + Pf1N1B4_3585 0.86 -3.0 3,998,303 + Pf1N1B4_3585 0.86 -4.0 3,998,768 + Pf1N1B4_3586 0.28 -1.1 3,998,860 + Pf1N1B4_3586 0.36 -0.1 3,998,860 + Pf1N1B4_3586 0.36 -4.4 3,998,900 - Pf1N1B4_3586 0.39 -1.9 3,998,939 + Pf1N1B4_3586 0.43 -0.1 3,998,939 + Pf1N1B4_3586 0.43 -0.1 3,998,939 + Pf1N1B4_3586 0.43 -0.1 3,998,940 - Pf1N1B4_3586 0.43 -0.9 3,998,940 - Pf1N1B4_3586 0.43 -1.5 3,999,140 + Pf1N1B4_3586 0.60 -0.1 3,999,144 - Pf1N1B4_3586 0.60 -0.1 3,999,167 - Pf1N1B4_3586 0.62 -0.1 3,999,281 + Pf1N1B4_3586 0.72 +0.0 3,999,281 + Pf1N1B4_3586 0.72 -1.7 3,999,338 + Pf1N1B4_3586 0.77 -0.6 3,999,338 + -2.4 3,999,338 + Pf1N1B4_3586 0.77 -6.0 3,999,339 - Pf1N1B4_3586 0.77 -1.4 3,999,339 - Pf1N1B4_3586 0.77 -0.1 3,999,339 - Pf1N1B4_3586 0.77 +0.7 3,999,431 + -1.6 3,999,431 + Pf1N1B4_3586 0.85 -0.1 3,999,431 + Pf1N1B4_3586 0.85 -0.1 3,999,432 - Pf1N1B4_3586 0.85 -0.1 3,999,432 - Pf1N1B4_3586 0.85 -4.6 3,999,432 - Pf1N1B4_3586 0.85 -0.1 3,999,433 + Pf1N1B4_3586 0.85 -0.1 3,999,434 - Pf1N1B4_3586 0.85 -0.1 3,999,434 - Pf1N1B4_3586 0.85 -0.1 3,999,434 - Pf1N1B4_3586 0.85 -0.1 3,999,434 - Pf1N1B4_3586 0.85 -3.0 3,999,434 - Pf1N1B4_3586 0.85 -1.9 3,999,434 - Pf1N1B4_3586 0.85 -0.1 3,999,434 - Pf1N1B4_3586 0.85 -0.1
Or see this region's nucleotide sequence