Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2989 and Pf1N1B4_2990 overlap by 4 nucleotides Pf1N1B4_2990 and Pf1N1B4_2991 are separated by 42 nucleotides Pf1N1B4_2991 and Pf1N1B4_2992 are separated by 100 nucleotides Pf1N1B4_2992 and Pf1N1B4_2993 are separated by 154 nucleotides
Pf1N1B4_2989 - NAD-dependent formate dehydrogenase beta subunit, at 3,357,859 to 3,359,421
_2989
Pf1N1B4_2990 - NAD-dependent formate dehydrogenase gamma subunit, at 3,359,418 to 3,359,894
_2990
Pf1N1B4_2991 - hypothetical protein, at 3,359,937 to 3,360,206
_2991
Pf1N1B4_2992 - Potassium channel protein, at 3,360,307 to 3,360,648
_2992
Pf1N1B4_2993 - hypothetical protein, at 3,360,803 to 3,361,132
_2993
Position (kb)
3359
3360 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3358.617 kb on + strand, within Pf1N1B4_2989 at 3358.617 kb on + strand, within Pf1N1B4_2989 at 3358.617 kb on + strand, within Pf1N1B4_2989 at 3358.617 kb on + strand at 3358.617 kb on + strand, within Pf1N1B4_2989 at 3358.617 kb on + strand, within Pf1N1B4_2989 at 3358.618 kb on - strand, within Pf1N1B4_2989 at 3358.618 kb on - strand, within Pf1N1B4_2989 at 3358.618 kb on - strand, within Pf1N1B4_2989 at 3358.618 kb on - strand, within Pf1N1B4_2989 at 3358.618 kb on - strand, within Pf1N1B4_2989 at 3358.618 kb on - strand, within Pf1N1B4_2989 at 3358.619 kb on + strand, within Pf1N1B4_2989 at 3358.619 kb on + strand, within Pf1N1B4_2989 at 3358.619 kb on + strand, within Pf1N1B4_2989 at 3358.619 kb on + strand, within Pf1N1B4_2989 at 3358.619 kb on + strand, within Pf1N1B4_2989 at 3358.619 kb on + strand, within Pf1N1B4_2989 at 3358.619 kb on + strand, within Pf1N1B4_2989 at 3358.620 kb on - strand, within Pf1N1B4_2989 at 3358.620 kb on - strand, within Pf1N1B4_2989 at 3358.620 kb on - strand, within Pf1N1B4_2989 at 3358.620 kb on - strand, within Pf1N1B4_2989 at 3358.620 kb on - strand, within Pf1N1B4_2989 at 3358.620 kb on - strand, within Pf1N1B4_2989 at 3358.620 kb on - strand, within Pf1N1B4_2989 at 3358.620 kb on - strand, within Pf1N1B4_2989 at 3358.620 kb on - strand, within Pf1N1B4_2989 at 3358.746 kb on - strand, within Pf1N1B4_2989 at 3358.760 kb on + strand, within Pf1N1B4_2989 at 3358.760 kb on + strand, within Pf1N1B4_2989 at 3358.760 kb on + strand, within Pf1N1B4_2989 at 3358.760 kb on + strand at 3358.760 kb on + strand, within Pf1N1B4_2989 at 3358.760 kb on + strand, within Pf1N1B4_2989 at 3358.760 kb on + strand, within Pf1N1B4_2989 at 3358.760 kb on + strand, within Pf1N1B4_2989 at 3358.760 kb on + strand, within Pf1N1B4_2989 at 3358.760 kb on + strand, within Pf1N1B4_2989 at 3358.760 kb on + strand, within Pf1N1B4_2989 at 3358.760 kb on + strand, within Pf1N1B4_2989 at 3358.761 kb on - strand, within Pf1N1B4_2989 at 3358.761 kb on - strand, within Pf1N1B4_2989 at 3358.761 kb on - strand, within Pf1N1B4_2989 at 3358.761 kb on - strand, within Pf1N1B4_2989 at 3358.761 kb on - strand, within Pf1N1B4_2989 at 3358.761 kb on - strand, within Pf1N1B4_2989 at 3358.761 kb on - strand at 3358.761 kb on - strand, within Pf1N1B4_2989 at 3358.761 kb on - strand, within Pf1N1B4_2989 at 3358.761 kb on - strand, within Pf1N1B4_2989 at 3358.761 kb on - strand, within Pf1N1B4_2989 at 3358.761 kb on - strand, within Pf1N1B4_2989 at 3358.761 kb on - strand, within Pf1N1B4_2989 at 3358.761 kb on - strand, within Pf1N1B4_2989 at 3358.766 kb on + strand, within Pf1N1B4_2989 at 3358.766 kb on + strand, within Pf1N1B4_2989 at 3358.766 kb on + strand, within Pf1N1B4_2989 at 3358.766 kb on + strand, within Pf1N1B4_2989 at 3358.766 kb on + strand, within Pf1N1B4_2989 at 3358.766 kb on + strand, within Pf1N1B4_2989 at 3358.766 kb on + strand, within Pf1N1B4_2989 at 3358.766 kb on + strand, within Pf1N1B4_2989 at 3358.772 kb on + strand, within Pf1N1B4_2989 at 3358.772 kb on + strand, within Pf1N1B4_2989 at 3358.773 kb on - strand, within Pf1N1B4_2989 at 3358.773 kb on - strand, within Pf1N1B4_2989 at 3358.895 kb on + strand, within Pf1N1B4_2989 at 3358.895 kb on + strand at 3358.896 kb on - strand, within Pf1N1B4_2989 at 3358.896 kb on - strand, within Pf1N1B4_2989 at 3358.896 kb on - strand, within Pf1N1B4_2989 at 3358.896 kb on - strand at 3358.896 kb on - strand, within Pf1N1B4_2989 at 3358.896 kb on - strand, within Pf1N1B4_2989 at 3358.896 kb on - strand, within Pf1N1B4_2989 at 3358.896 kb on - strand, within Pf1N1B4_2989 at 3358.896 kb on - strand, within Pf1N1B4_2989 at 3358.896 kb on - strand, within Pf1N1B4_2989 at 3358.896 kb on - strand, within Pf1N1B4_2989 at 3358.896 kb on - strand, within Pf1N1B4_2989 at 3359.405 kb on + strand at 3359.406 kb on - strand at 3359.406 kb on - strand at 3359.406 kb on - strand at 3359.533 kb on + strand, within Pf1N1B4_2990 at 3359.533 kb on + strand, within Pf1N1B4_2990 at 3359.533 kb on + strand, within Pf1N1B4_2990 at 3359.534 kb on - strand, within Pf1N1B4_2990 at 3359.534 kb on - strand, within Pf1N1B4_2990 at 3359.534 kb on - strand, within Pf1N1B4_2990 at 3359.666 kb on + strand, within Pf1N1B4_2990 at 3359.666 kb on + strand, within Pf1N1B4_2990 at 3359.701 kb on + strand, within Pf1N1B4_2990 at 3359.701 kb on + strand, within Pf1N1B4_2990 at 3359.701 kb on + strand, within Pf1N1B4_2990 at 3359.701 kb on + strand, within Pf1N1B4_2990 at 3359.702 kb on - strand, within Pf1N1B4_2990 at 3359.702 kb on - strand, within Pf1N1B4_2990 at 3359.702 kb on - strand, within Pf1N1B4_2990 at 3359.702 kb on - strand, within Pf1N1B4_2990 at 3359.702 kb on - strand, within Pf1N1B4_2990 at 3359.702 kb on - strand, within Pf1N1B4_2990 at 3359.702 kb on - strand at 3359.702 kb on - strand, within Pf1N1B4_2990 at 3359.702 kb on - strand, within Pf1N1B4_2990 at 3359.702 kb on - strand, within Pf1N1B4_2990 at 3359.702 kb on - strand, within Pf1N1B4_2990 at 3359.782 kb on + strand, within Pf1N1B4_2990 at 3359.782 kb on + strand, within Pf1N1B4_2990 at 3359.782 kb on + strand at 3359.868 kb on - strand at 3359.894 kb on + strand at 3359.894 kb on + strand at 3359.894 kb on + strand at 3359.895 kb on - strand at 3359.895 kb on - strand at 3359.895 kb on - strand at 3360.068 kb on + strand, within Pf1N1B4_2991 at 3360.145 kb on + strand, within Pf1N1B4_2991 at 3360.145 kb on + strand, within Pf1N1B4_2991 at 3360.146 kb on - strand, within Pf1N1B4_2991 at 3360.146 kb on - strand, within Pf1N1B4_2991 at 3360.146 kb on - strand, within Pf1N1B4_2991 at 3360.146 kb on - strand, within Pf1N1B4_2991 at 3360.146 kb on - strand, within Pf1N1B4_2991 at 3360.151 kb on + strand, within Pf1N1B4_2991 at 3360.151 kb on + strand, within Pf1N1B4_2991 at 3360.151 kb on + strand, within Pf1N1B4_2991 at 3360.151 kb on + strand, within Pf1N1B4_2991 at 3360.152 kb on - strand, within Pf1N1B4_2991 at 3360.186 kb on + strand at 3360.186 kb on + strand at 3360.186 kb on + strand at 3360.186 kb on + strand at 3360.197 kb on + strand at 3360.198 kb on - strand at 3360.252 kb on - strand at 3360.287 kb on - strand at 3360.346 kb on + strand, within Pf1N1B4_2992 at 3360.347 kb on - strand, within Pf1N1B4_2992 at 3360.347 kb on - strand, within Pf1N1B4_2992 at 3360.347 kb on - strand at 3360.347 kb on - strand, within Pf1N1B4_2992 at 3360.347 kb on - strand, within Pf1N1B4_2992 at 3360.364 kb on + strand, within Pf1N1B4_2992 at 3360.364 kb on + strand, within Pf1N1B4_2992 at 3360.365 kb on - strand, within Pf1N1B4_2992 at 3360.365 kb on - strand, within Pf1N1B4_2992 at 3360.365 kb on - strand, within Pf1N1B4_2992 at 3360.365 kb on - strand, within Pf1N1B4_2992 at 3360.365 kb on - strand, within Pf1N1B4_2992 at 3360.469 kb on + strand, within Pf1N1B4_2992 at 3360.469 kb on + strand, within Pf1N1B4_2992 at 3360.469 kb on + strand, within Pf1N1B4_2992 at 3360.469 kb on + strand, within Pf1N1B4_2992 at 3360.469 kb on + strand, within Pf1N1B4_2992 at 3360.469 kb on + strand, within Pf1N1B4_2992 at 3360.469 kb on + strand, within Pf1N1B4_2992 at 3360.469 kb on + strand, within Pf1N1B4_2992 at 3360.470 kb on - strand, within Pf1N1B4_2992 at 3360.470 kb on - strand, within Pf1N1B4_2992 at 3360.470 kb on - strand, within Pf1N1B4_2992 at 3360.470 kb on - strand, within Pf1N1B4_2992 at 3360.470 kb on - strand, within Pf1N1B4_2992 at 3360.470 kb on - strand, within Pf1N1B4_2992 at 3360.470 kb on - strand, within Pf1N1B4_2992 at 3360.470 kb on - strand, within Pf1N1B4_2992 at 3360.584 kb on + strand, within Pf1N1B4_2992 at 3360.585 kb on - strand, within Pf1N1B4_2992 at 3360.646 kb on + strand at 3360.646 kb on + strand at 3360.646 kb on + strand at 3360.646 kb on + strand at 3360.722 kb on + strand at 3360.722 kb on + strand at 3360.787 kb on - strand at 3360.829 kb on + strand at 3360.830 kb on - strand at 3360.830 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 3,358,617 + Pf1N1B4_2989 0.48 -0.1 3,358,617 + Pf1N1B4_2989 0.48 -1.2 3,358,617 + Pf1N1B4_2989 0.48 -0.1 3,358,617 + -0.1 3,358,617 + Pf1N1B4_2989 0.48 -2.4 3,358,617 + Pf1N1B4_2989 0.48 -1.5 3,358,618 - Pf1N1B4_2989 0.49 -0.1 3,358,618 - Pf1N1B4_2989 0.49 -0.1 3,358,618 - Pf1N1B4_2989 0.49 -0.1 3,358,618 - Pf1N1B4_2989 0.49 -0.2 3,358,618 - Pf1N1B4_2989 0.49 -0.1 3,358,618 - Pf1N1B4_2989 0.49 -0.1 3,358,619 + Pf1N1B4_2989 0.49 -0.1 3,358,619 + Pf1N1B4_2989 0.49 -0.1 3,358,619 + Pf1N1B4_2989 0.49 -0.1 3,358,619 + Pf1N1B4_2989 0.49 -1.3 3,358,619 + Pf1N1B4_2989 0.49 -1.4 3,358,619 + Pf1N1B4_2989 0.49 -1.7 3,358,619 + Pf1N1B4_2989 0.49 -1.0 3,358,620 - Pf1N1B4_2989 0.49 -0.1 3,358,620 - Pf1N1B4_2989 0.49 -0.1 3,358,620 - Pf1N1B4_2989 0.49 -0.1 3,358,620 - Pf1N1B4_2989 0.49 -0.1 3,358,620 - Pf1N1B4_2989 0.49 -0.1 3,358,620 - Pf1N1B4_2989 0.49 -2.5 3,358,620 - Pf1N1B4_2989 0.49 -0.1 3,358,620 - Pf1N1B4_2989 0.49 -0.9 3,358,620 - Pf1N1B4_2989 0.49 -0.1 3,358,746 - Pf1N1B4_2989 0.57 -0.1 3,358,760 + Pf1N1B4_2989 0.58 -5.5 3,358,760 + Pf1N1B4_2989 0.58 -2.0 3,358,760 + Pf1N1B4_2989 0.58 -1.2 3,358,760 + -2.7 3,358,760 + Pf1N1B4_2989 0.58 -0.1 3,358,760 + Pf1N1B4_2989 0.58 -3.2 3,358,760 + Pf1N1B4_2989 0.58 -0.1 3,358,760 + Pf1N1B4_2989 0.58 -0.1 3,358,760 + Pf1N1B4_2989 0.58 -0.1 3,358,760 + Pf1N1B4_2989 0.58 -0.1 3,358,760 + Pf1N1B4_2989 0.58 -1.8 3,358,760 + Pf1N1B4_2989 0.58 -0.1 3,358,761 - Pf1N1B4_2989 0.58 -0.1 3,358,761 - Pf1N1B4_2989 0.58 -0.1 3,358,761 - Pf1N1B4_2989 0.58 -2.1 3,358,761 - Pf1N1B4_2989 0.58 -0.1 3,358,761 - Pf1N1B4_2989 0.58 -2.7 3,358,761 - Pf1N1B4_2989 0.58 -1.3 3,358,761 - -5.6 3,358,761 - Pf1N1B4_2989 0.58 -0.1 3,358,761 - Pf1N1B4_2989 0.58 -0.1 3,358,761 - Pf1N1B4_2989 0.58 -1.4 3,358,761 - Pf1N1B4_2989 0.58 -0.1 3,358,761 - Pf1N1B4_2989 0.58 -0.1 3,358,761 - Pf1N1B4_2989 0.58 -0.1 3,358,761 - Pf1N1B4_2989 0.58 -1.9 3,358,766 + Pf1N1B4_2989 0.58 -2.1 3,358,766 + Pf1N1B4_2989 0.58 -1.6 3,358,766 + Pf1N1B4_2989 0.58 -0.1 3,358,766 + Pf1N1B4_2989 0.58 -2.2 3,358,766 + Pf1N1B4_2989 0.58 -0.1 3,358,766 + Pf1N1B4_2989 0.58 -1.2 3,358,766 + Pf1N1B4_2989 0.58 -0.1 3,358,766 + Pf1N1B4_2989 0.58 -0.1 3,358,772 + Pf1N1B4_2989 0.58 -0.1 3,358,772 + Pf1N1B4_2989 0.58 -0.1 3,358,773 - Pf1N1B4_2989 0.58 -0.1 3,358,773 - Pf1N1B4_2989 0.58 -3.9 3,358,895 + Pf1N1B4_2989 0.66 -1.7 3,358,895 + -2.1 3,358,896 - Pf1N1B4_2989 0.66 -0.1 3,358,896 - Pf1N1B4_2989 0.66 -0.1 3,358,896 - Pf1N1B4_2989 0.66 -0.1 3,358,896 - -3.4 3,358,896 - Pf1N1B4_2989 0.66 -0.1 3,358,896 - Pf1N1B4_2989 0.66 -0.1 3,358,896 - Pf1N1B4_2989 0.66 -2.2 3,358,896 - Pf1N1B4_2989 0.66 -0.1 3,358,896 - Pf1N1B4_2989 0.66 -5.0 3,358,896 - Pf1N1B4_2989 0.66 -0.1 3,358,896 - Pf1N1B4_2989 0.66 -0.7 3,358,896 - Pf1N1B4_2989 0.66 -0.1 3,359,405 + -0.7 3,359,406 - -0.1 3,359,406 - -1.9 3,359,406 - -0.1 3,359,533 + Pf1N1B4_2990 0.24 -0.1 3,359,533 + Pf1N1B4_2990 0.24 -0.1 3,359,533 + Pf1N1B4_2990 0.24 -0.2 3,359,534 - Pf1N1B4_2990 0.24 -1.5 3,359,534 - Pf1N1B4_2990 0.24 -0.1 3,359,534 - Pf1N1B4_2990 0.24 -0.1 3,359,666 + Pf1N1B4_2990 0.52 -2.0 3,359,666 + Pf1N1B4_2990 0.52 -0.1 3,359,701 + Pf1N1B4_2990 0.59 -0.1 3,359,701 + Pf1N1B4_2990 0.59 -0.1 3,359,701 + Pf1N1B4_2990 0.59 -1.7 3,359,701 + Pf1N1B4_2990 0.59 -2.1 3,359,702 - Pf1N1B4_2990 0.60 -4.8 3,359,702 - Pf1N1B4_2990 0.60 -0.1 3,359,702 - Pf1N1B4_2990 0.60 -2.7 3,359,702 - Pf1N1B4_2990 0.60 -0.1 3,359,702 - Pf1N1B4_2990 0.60 -0.1 3,359,702 - Pf1N1B4_2990 0.60 -0.1 3,359,702 - -1.3 3,359,702 - Pf1N1B4_2990 0.60 -0.1 3,359,702 - Pf1N1B4_2990 0.60 -1.3 3,359,702 - Pf1N1B4_2990 0.60 -0.1 3,359,702 - Pf1N1B4_2990 0.60 -0.1 3,359,782 + Pf1N1B4_2990 0.76 -5.4 3,359,782 + Pf1N1B4_2990 0.76 -0.1 3,359,782 + -2.8 3,359,868 - -3.7 3,359,894 + -1.0 3,359,894 + -0.1 3,359,894 + -0.1 3,359,895 - -2.1 3,359,895 - -0.1 3,359,895 - -2.2 3,360,068 + Pf1N1B4_2991 0.49 -0.1 3,360,145 + Pf1N1B4_2991 0.77 -0.1 3,360,145 + Pf1N1B4_2991 0.77 -1.1 3,360,146 - Pf1N1B4_2991 0.77 -0.1 3,360,146 - Pf1N1B4_2991 0.77 -0.1 3,360,146 - Pf1N1B4_2991 0.77 -0.1 3,360,146 - Pf1N1B4_2991 0.77 -3.9 3,360,146 - Pf1N1B4_2991 0.77 -0.1 3,360,151 + Pf1N1B4_2991 0.79 -0.8 3,360,151 + Pf1N1B4_2991 0.79 -5.2 3,360,151 + Pf1N1B4_2991 0.79 -0.1 3,360,151 + Pf1N1B4_2991 0.79 -0.1 3,360,152 - Pf1N1B4_2991 0.80 -2.1 3,360,186 + -1.3 3,360,186 + -0.9 3,360,186 + -0.1 3,360,186 + +0.7 3,360,197 + -2.7 3,360,198 - -0.1 3,360,252 - -0.1 3,360,287 - -1.8 3,360,346 + Pf1N1B4_2992 0.11 -2.4 3,360,347 - Pf1N1B4_2992 0.12 -0.1 3,360,347 - Pf1N1B4_2992 0.12 -1.1 3,360,347 - -4.3 3,360,347 - Pf1N1B4_2992 0.12 -0.1 3,360,347 - Pf1N1B4_2992 0.12 -0.1 3,360,364 + Pf1N1B4_2992 0.17 -0.1 3,360,364 + Pf1N1B4_2992 0.17 -0.1 3,360,365 - Pf1N1B4_2992 0.17 -4.9 3,360,365 - Pf1N1B4_2992 0.17 -0.1 3,360,365 - Pf1N1B4_2992 0.17 -0.1 3,360,365 - Pf1N1B4_2992 0.17 -0.1 3,360,365 - Pf1N1B4_2992 0.17 -0.1 3,360,469 + Pf1N1B4_2992 0.47 -0.1 3,360,469 + Pf1N1B4_2992 0.47 -0.1 3,360,469 + Pf1N1B4_2992 0.47 -0.9 3,360,469 + Pf1N1B4_2992 0.47 -0.1 3,360,469 + Pf1N1B4_2992 0.47 -0.1 3,360,469 + Pf1N1B4_2992 0.47 -2.1 3,360,469 + Pf1N1B4_2992 0.47 -0.1 3,360,469 + Pf1N1B4_2992 0.47 -0.1 3,360,470 - Pf1N1B4_2992 0.48 -1.5 3,360,470 - Pf1N1B4_2992 0.48 -3.5 3,360,470 - Pf1N1B4_2992 0.48 -0.1 3,360,470 - Pf1N1B4_2992 0.48 -0.1 3,360,470 - Pf1N1B4_2992 0.48 -0.1 3,360,470 - Pf1N1B4_2992 0.48 -0.1 3,360,470 - Pf1N1B4_2992 0.48 -2.8 3,360,470 - Pf1N1B4_2992 0.48 -0.1 3,360,584 + Pf1N1B4_2992 0.81 -0.1 3,360,585 - Pf1N1B4_2992 0.81 -0.1 3,360,646 + -1.9 3,360,646 + -0.1 3,360,646 + -1.0 3,360,646 + -3.7 3,360,722 + -1.4 3,360,722 + -0.1 3,360,787 - -1.1 3,360,829 + -2.4 3,360,830 - -2.2 3,360,830 - -3.5
Or see this region's nucleotide sequence