Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2741

Experiment: Nitrate reduction

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2739 and Pf1N1B4_2740 are separated by 105 nucleotidesPf1N1B4_2740 and Pf1N1B4_2741 overlap by 4 nucleotidesPf1N1B4_2741 and Pf1N1B4_2742 are separated by 141 nucleotides Pf1N1B4_2739 - acetyltransferase, GNAT family, at 3,078,266 to 3,078,835 _2739 Pf1N1B4_2740 - Acyl-CoA thioesterase II (EC 3.1.2.-), at 3,078,941 to 3,079,810 _2740 Pf1N1B4_2741 - Hydrolase in polyol utilization gene cluster, haloacid dehalogenase-like family, at 3,079,807 to 3,080,400 _2741 Pf1N1B4_2742 - putative exported protein, at 3,080,542 to 3,081,501 _2742 Position (kb) 3079 3080 3081Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 3078.833 kb on + strandat 3078.851 kb on + strandat 3078.851 kb on + strandat 3078.851 kb on + strandat 3078.851 kb on + strandat 3078.852 kb on - strandat 3078.852 kb on - strandat 3078.852 kb on - strandat 3078.852 kb on - strandat 3078.852 kb on - strandat 3078.879 kb on + strandat 3079.057 kb on - strand, within Pf1N1B4_2740at 3079.127 kb on + strand, within Pf1N1B4_2740at 3079.128 kb on - strand, within Pf1N1B4_2740at 3079.128 kb on - strand, within Pf1N1B4_2740at 3079.166 kb on + strand, within Pf1N1B4_2740at 3079.268 kb on + strand, within Pf1N1B4_2740at 3079.311 kb on + strand, within Pf1N1B4_2740at 3079.312 kb on - strand, within Pf1N1B4_2740at 3079.312 kb on - strand, within Pf1N1B4_2740at 3079.312 kb on - strand, within Pf1N1B4_2740at 3079.445 kb on + strand, within Pf1N1B4_2740at 3079.445 kb on + strand, within Pf1N1B4_2740at 3079.446 kb on - strand, within Pf1N1B4_2740at 3079.475 kb on + strand, within Pf1N1B4_2740at 3079.475 kb on + strand, within Pf1N1B4_2740at 3079.475 kb on + strand, within Pf1N1B4_2740at 3079.475 kb on + strand, within Pf1N1B4_2740at 3079.475 kb on + strand, within Pf1N1B4_2740at 3079.476 kb on - strand, within Pf1N1B4_2740at 3079.476 kb on - strand, within Pf1N1B4_2740at 3079.476 kb on - strand, within Pf1N1B4_2740at 3079.476 kb on - strand, within Pf1N1B4_2740at 3079.476 kb on - strand, within Pf1N1B4_2740at 3079.529 kb on + strand, within Pf1N1B4_2740at 3079.529 kb on + strand, within Pf1N1B4_2740at 3079.529 kb on + strand, within Pf1N1B4_2740at 3079.529 kb on + strand, within Pf1N1B4_2740at 3079.529 kb on + strand, within Pf1N1B4_2740at 3079.529 kb on + strand, within Pf1N1B4_2740at 3079.529 kb on + strand, within Pf1N1B4_2740at 3079.529 kb on + strand, within Pf1N1B4_2740at 3079.529 kb on + strand, within Pf1N1B4_2740at 3079.529 kb on + strand, within Pf1N1B4_2740at 3079.530 kb on - strand, within Pf1N1B4_2740at 3079.530 kb on - strand, within Pf1N1B4_2740at 3079.530 kb on - strand, within Pf1N1B4_2740at 3079.530 kb on - strand, within Pf1N1B4_2740at 3079.530 kb on - strand, within Pf1N1B4_2740at 3079.680 kb on - strand, within Pf1N1B4_2740at 3080.118 kb on - strand, within Pf1N1B4_2741at 3080.159 kb on + strand, within Pf1N1B4_2741at 3080.159 kb on + strand, within Pf1N1B4_2741at 3080.159 kb on + strand, within Pf1N1B4_2741at 3080.159 kb on + strand, within Pf1N1B4_2741at 3080.160 kb on - strand, within Pf1N1B4_2741at 3080.160 kb on - strand, within Pf1N1B4_2741at 3080.405 kb on + strandat 3080.405 kb on + strandat 3080.405 kb on + strandat 3080.405 kb on + strandat 3080.405 kb on + strandat 3080.405 kb on + strandat 3080.405 kb on + strandat 3080.405 kb on + strandat 3080.405 kb on + strandat 3080.406 kb on - strandat 3080.406 kb on - strandat 3080.406 kb on - strandat 3080.406 kb on - strandat 3080.406 kb on - strandat 3080.406 kb on - strandat 3080.478 kb on + strandat 3080.580 kb on + strandat 3080.581 kb on - strandat 3080.727 kb on + strand, within Pf1N1B4_2742at 3080.770 kb on + strand, within Pf1N1B4_2742at 3080.771 kb on - strand, within Pf1N1B4_2742at 3080.771 kb on - strand, within Pf1N1B4_2742at 3080.771 kb on - strand, within Pf1N1B4_2742at 3080.771 kb on - strand, within Pf1N1B4_2742at 3080.774 kb on + strand, within Pf1N1B4_2742at 3080.774 kb on + strand, within Pf1N1B4_2742at 3080.774 kb on + strand, within Pf1N1B4_2742at 3080.774 kb on + strand, within Pf1N1B4_2742at 3080.775 kb on - strand, within Pf1N1B4_2742at 3080.775 kb on - strand, within Pf1N1B4_2742at 3080.775 kb on - strand, within Pf1N1B4_2742at 3080.775 kb on - strand, within Pf1N1B4_2742at 3080.795 kb on + strand, within Pf1N1B4_2742at 3080.795 kb on + strand, within Pf1N1B4_2742at 3080.795 kb on + strand, within Pf1N1B4_2742at 3080.834 kb on + strand, within Pf1N1B4_2742at 3080.834 kb on + strand, within Pf1N1B4_2742at 3080.835 kb on - strand, within Pf1N1B4_2742at 3080.859 kb on - strand, within Pf1N1B4_2742at 3080.859 kb on - strand, within Pf1N1B4_2742at 3080.924 kb on + strand, within Pf1N1B4_2742at 3080.924 kb on + strand, within Pf1N1B4_2742at 3080.924 kb on + strand, within Pf1N1B4_2742at 3080.924 kb on + strand, within Pf1N1B4_2742at 3080.924 kb on + strand, within Pf1N1B4_2742at 3080.924 kb on + strand, within Pf1N1B4_2742at 3080.924 kb on + strand, within Pf1N1B4_2742at 3080.925 kb on - strand, within Pf1N1B4_2742at 3080.925 kb on - strand, within Pf1N1B4_2742at 3080.925 kb on - strand, within Pf1N1B4_2742at 3080.925 kb on - strand, within Pf1N1B4_2742at 3080.925 kb on - strand, within Pf1N1B4_2742at 3080.930 kb on + strand, within Pf1N1B4_2742at 3080.930 kb on + strand, within Pf1N1B4_2742at 3080.930 kb on + strand, within Pf1N1B4_2742at 3080.931 kb on - strand, within Pf1N1B4_2742at 3080.931 kb on - strand, within Pf1N1B4_2742at 3080.931 kb on - strand, within Pf1N1B4_2742at 3080.954 kb on + strand, within Pf1N1B4_2742at 3080.954 kb on + strand, within Pf1N1B4_2742at 3080.954 kb on + strand, within Pf1N1B4_2742at 3080.955 kb on - strand, within Pf1N1B4_2742at 3080.955 kb on - strand, within Pf1N1B4_2742at 3081.136 kb on - strand, within Pf1N1B4_2742at 3081.188 kb on - strand, within Pf1N1B4_2742at 3081.188 kb on - strand, within Pf1N1B4_2742at 3081.308 kb on + strand, within Pf1N1B4_2742at 3081.309 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction
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3,078,833 + -0.0
3,078,851 + -1.1
3,078,851 + -0.0
3,078,851 + -0.0
3,078,851 + -0.0
3,078,852 - -0.4
3,078,852 - -1.7
3,078,852 - -0.0
3,078,852 - -0.2
3,078,852 - -0.0
3,078,879 + -0.0
3,079,057 - Pf1N1B4_2740 0.13 -4.5
3,079,127 + Pf1N1B4_2740 0.21 -1.5
3,079,128 - Pf1N1B4_2740 0.21 -0.0
3,079,128 - Pf1N1B4_2740 0.21 -2.0
3,079,166 + Pf1N1B4_2740 0.26 -1.1
3,079,268 + Pf1N1B4_2740 0.38 -0.0
3,079,311 + Pf1N1B4_2740 0.43 -0.0
3,079,312 - Pf1N1B4_2740 0.43 -0.9
3,079,312 - Pf1N1B4_2740 0.43 -1.3
3,079,312 - Pf1N1B4_2740 0.43 -0.0
3,079,445 + Pf1N1B4_2740 0.58 -0.0
3,079,445 + Pf1N1B4_2740 0.58 -0.0
3,079,446 - Pf1N1B4_2740 0.58 -0.0
3,079,475 + Pf1N1B4_2740 0.61 -0.0
3,079,475 + Pf1N1B4_2740 0.61 -3.8
3,079,475 + Pf1N1B4_2740 0.61 -2.5
3,079,475 + Pf1N1B4_2740 0.61 -0.0
3,079,475 + Pf1N1B4_2740 0.61 -0.0
3,079,476 - Pf1N1B4_2740 0.61 -0.0
3,079,476 - Pf1N1B4_2740 0.61 -0.0
3,079,476 - Pf1N1B4_2740 0.61 -4.0
3,079,476 - Pf1N1B4_2740 0.61 -1.2
3,079,476 - Pf1N1B4_2740 0.61 -0.0
3,079,529 + Pf1N1B4_2740 0.68 -1.8
3,079,529 + Pf1N1B4_2740 0.68 -0.0
3,079,529 + Pf1N1B4_2740 0.68 -0.0
3,079,529 + Pf1N1B4_2740 0.68 -0.0
3,079,529 + Pf1N1B4_2740 0.68 -0.5
3,079,529 + Pf1N1B4_2740 0.68 -0.0
3,079,529 + Pf1N1B4_2740 0.68 -0.0
3,079,529 + Pf1N1B4_2740 0.68 -0.0
3,079,529 + Pf1N1B4_2740 0.68 -0.0
3,079,529 + Pf1N1B4_2740 0.68 -2.1
3,079,530 - Pf1N1B4_2740 0.68 -0.0
3,079,530 - Pf1N1B4_2740 0.68 -0.0
3,079,530 - Pf1N1B4_2740 0.68 -1.3
3,079,530 - Pf1N1B4_2740 0.68 -0.0
3,079,530 - Pf1N1B4_2740 0.68 -0.0
3,079,680 - Pf1N1B4_2740 0.85 -0.0
3,080,118 - Pf1N1B4_2741 0.52 -0.0
3,080,159 + Pf1N1B4_2741 0.59 -3.8
3,080,159 + Pf1N1B4_2741 0.59 -1.1
3,080,159 + Pf1N1B4_2741 0.59 -1.2
3,080,159 + Pf1N1B4_2741 0.59 -0.0
3,080,160 - Pf1N1B4_2741 0.59 -0.0
3,080,160 - Pf1N1B4_2741 0.59 -3.0
3,080,405 + -0.0
3,080,405 + -0.0
3,080,405 + -0.0
3,080,405 + -0.0
3,080,405 + -0.0
3,080,405 + -0.0
3,080,405 + -1.3
3,080,405 + -4.3
3,080,405 + -0.4
3,080,406 - -0.0
3,080,406 - -0.0
3,080,406 - -1.9
3,080,406 - -0.0
3,080,406 - -0.0
3,080,406 - -0.0
3,080,478 + -0.0
3,080,580 + -1.1
3,080,581 - -0.0
3,080,727 + Pf1N1B4_2742 0.19 -0.0
3,080,770 + Pf1N1B4_2742 0.24 -0.0
3,080,771 - Pf1N1B4_2742 0.24 -4.4
3,080,771 - Pf1N1B4_2742 0.24 -1.6
3,080,771 - Pf1N1B4_2742 0.24 -0.0
3,080,771 - Pf1N1B4_2742 0.24 -0.0
3,080,774 + Pf1N1B4_2742 0.24 -0.0
3,080,774 + Pf1N1B4_2742 0.24 -0.0
3,080,774 + Pf1N1B4_2742 0.24 -4.3
3,080,774 + Pf1N1B4_2742 0.24 -0.0
3,080,775 - Pf1N1B4_2742 0.24 -4.1
3,080,775 - Pf1N1B4_2742 0.24 -2.1
3,080,775 - Pf1N1B4_2742 0.24 -0.0
3,080,775 - Pf1N1B4_2742 0.24 -5.3
3,080,795 + Pf1N1B4_2742 0.26 -0.0
3,080,795 + Pf1N1B4_2742 0.26 -4.2
3,080,795 + Pf1N1B4_2742 0.26 -0.0
3,080,834 + Pf1N1B4_2742 0.30 -0.0
3,080,834 + Pf1N1B4_2742 0.30 -1.0
3,080,835 - Pf1N1B4_2742 0.31 -0.0
3,080,859 - Pf1N1B4_2742 0.33 -0.0
3,080,859 - Pf1N1B4_2742 0.33 -1.2
3,080,924 + Pf1N1B4_2742 0.40 -0.0
3,080,924 + Pf1N1B4_2742 0.40 -0.0
3,080,924 + Pf1N1B4_2742 0.40 -0.0
3,080,924 + Pf1N1B4_2742 0.40 -0.0
3,080,924 + Pf1N1B4_2742 0.40 -2.1
3,080,924 + Pf1N1B4_2742 0.40 -0.0
3,080,924 + Pf1N1B4_2742 0.40 -0.0
3,080,925 - Pf1N1B4_2742 0.40 -1.3
3,080,925 - Pf1N1B4_2742 0.40 -0.0
3,080,925 - Pf1N1B4_2742 0.40 -3.5
3,080,925 - Pf1N1B4_2742 0.40 -0.0
3,080,925 - Pf1N1B4_2742 0.40 -0.0
3,080,930 + Pf1N1B4_2742 0.40 -0.0
3,080,930 + Pf1N1B4_2742 0.40 -0.0
3,080,930 + Pf1N1B4_2742 0.40 -1.2
3,080,931 - Pf1N1B4_2742 0.41 -0.0
3,080,931 - Pf1N1B4_2742 0.41 -0.0
3,080,931 - Pf1N1B4_2742 0.41 -0.0
3,080,954 + Pf1N1B4_2742 0.43 -0.0
3,080,954 + Pf1N1B4_2742 0.43 -0.0
3,080,954 + Pf1N1B4_2742 0.43 -0.0
3,080,955 - Pf1N1B4_2742 0.43 -0.0
3,080,955 - Pf1N1B4_2742 0.43 -0.0
3,081,136 - Pf1N1B4_2742 0.62 -0.0
3,081,188 - Pf1N1B4_2742 0.67 -0.0
3,081,188 - Pf1N1B4_2742 0.67 -0.5
3,081,308 + Pf1N1B4_2742 0.80 -0.0
3,081,309 - -0.4

Or see this region's nucleotide sequence