Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2729 and Pf1N1B4_2730 are separated by 88 nucleotides Pf1N1B4_2730 and Pf1N1B4_2731 are separated by 178 nucleotides Pf1N1B4_2731 and Pf1N1B4_2732 are separated by 142 nucleotides Pf1N1B4_2732 and Pf1N1B4_2733 are separated by 22 nucleotides
Pf1N1B4_2729 - endonuclease/exonuclease/phosphatase family protein, at 3,068,505 to 3,069,587
_2729
Pf1N1B4_2730 - FIG00958173: hypothetical protein, at 3,069,676 to 3,070,350
_2730
Pf1N1B4_2731 - Uncharacterized protein conserved in bacteria, at 3,070,529 to 3,071,281
_2731
Pf1N1B4_2732 - FIG00954572: hypothetical protein, at 3,071,424 to 3,071,903
_2732
Pf1N1B4_2733 - DNA polymerase III alpha subunit (EC 2.7.7.7), at 3,071,926 to 3,072,537
_2733
Position (kb)
3070
3071
3072 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3069.597 kb on + strand at 3069.598 kb on - strand at 3069.598 kb on - strand at 3069.599 kb on + strand at 3069.599 kb on + strand at 3069.599 kb on + strand at 3069.600 kb on - strand at 3069.600 kb on - strand at 3069.600 kb on - strand at 3069.605 kb on + strand at 3069.656 kb on + strand at 3069.816 kb on - strand, within Pf1N1B4_2730 at 3069.962 kb on + strand, within Pf1N1B4_2730 at 3070.010 kb on + strand at 3070.010 kb on + strand, within Pf1N1B4_2730 at 3070.010 kb on + strand, within Pf1N1B4_2730 at 3070.010 kb on + strand, within Pf1N1B4_2730 at 3070.010 kb on + strand, within Pf1N1B4_2730 at 3070.011 kb on - strand, within Pf1N1B4_2730 at 3070.163 kb on + strand, within Pf1N1B4_2730 at 3070.163 kb on + strand, within Pf1N1B4_2730 at 3070.163 kb on + strand, within Pf1N1B4_2730 at 3070.163 kb on + strand, within Pf1N1B4_2730 at 3070.163 kb on + strand, within Pf1N1B4_2730 at 3070.163 kb on + strand, within Pf1N1B4_2730 at 3070.164 kb on - strand, within Pf1N1B4_2730 at 3070.164 kb on - strand, within Pf1N1B4_2730 at 3070.164 kb on - strand, within Pf1N1B4_2730 at 3070.164 kb on - strand, within Pf1N1B4_2730 at 3070.164 kb on - strand, within Pf1N1B4_2730 at 3070.164 kb on - strand, within Pf1N1B4_2730 at 3070.165 kb on + strand, within Pf1N1B4_2730 at 3070.165 kb on + strand, within Pf1N1B4_2730 at 3070.192 kb on + strand, within Pf1N1B4_2730 at 3070.192 kb on + strand, within Pf1N1B4_2730 at 3070.193 kb on - strand, within Pf1N1B4_2730 at 3070.193 kb on - strand, within Pf1N1B4_2730 at 3070.193 kb on - strand, within Pf1N1B4_2730 at 3070.193 kb on - strand, within Pf1N1B4_2730 at 3070.316 kb on + strand at 3070.316 kb on + strand at 3070.316 kb on + strand at 3070.317 kb on - strand at 3070.317 kb on - strand at 3070.317 kb on - strand at 3070.317 kb on - strand at 3070.317 kb on - strand at 3070.317 kb on - strand at 3070.333 kb on + strand at 3070.334 kb on - strand at 3070.334 kb on - strand at 3070.334 kb on - strand at 3070.334 kb on - strand at 3070.334 kb on - strand at 3070.334 kb on - strand at 3070.334 kb on - strand at 3070.334 kb on - strand at 3070.334 kb on - strand at 3070.388 kb on + strand at 3070.418 kb on + strand at 3070.418 kb on + strand at 3070.418 kb on + strand at 3070.418 kb on + strand at 3070.419 kb on - strand at 3070.419 kb on - strand at 3070.419 kb on - strand at 3070.452 kb on - strand at 3070.503 kb on + strand at 3070.517 kb on + strand at 3070.517 kb on + strand at 3070.517 kb on + strand at 3070.517 kb on + strand at 3070.517 kb on + strand at 3070.518 kb on - strand at 3070.518 kb on - strand at 3070.665 kb on - strand, within Pf1N1B4_2731 at 3070.665 kb on - strand, within Pf1N1B4_2731 at 3070.973 kb on + strand, within Pf1N1B4_2731 at 3070.974 kb on - strand, within Pf1N1B4_2731 at 3070.974 kb on - strand, within Pf1N1B4_2731 at 3070.974 kb on - strand, within Pf1N1B4_2731 at 3070.974 kb on - strand, within Pf1N1B4_2731 at 3071.072 kb on - strand, within Pf1N1B4_2731 at 3071.191 kb on + strand, within Pf1N1B4_2731 at 3071.192 kb on - strand, within Pf1N1B4_2731 at 3071.192 kb on - strand, within Pf1N1B4_2731 at 3071.192 kb on - strand, within Pf1N1B4_2731 at 3071.228 kb on + strand at 3071.229 kb on - strand at 3071.229 kb on - strand at 3071.229 kb on - strand at 3071.230 kb on + strand at 3071.355 kb on + strand at 3071.355 kb on + strand at 3071.355 kb on + strand at 3071.356 kb on - strand at 3071.420 kb on + strand at 3071.420 kb on + strand at 3071.420 kb on + strand at 3071.420 kb on + strand at 3071.420 kb on + strand at 3071.420 kb on + strand at 3071.420 kb on + strand at 3071.420 kb on + strand at 3071.420 kb on + strand at 3071.421 kb on - strand at 3071.421 kb on - strand at 3071.421 kb on - strand at 3071.421 kb on - strand at 3071.421 kb on - strand at 3071.421 kb on - strand at 3071.421 kb on - strand at 3071.421 kb on - strand at 3071.463 kb on + strand at 3071.463 kb on + strand at 3071.464 kb on - strand at 3071.464 kb on - strand at 3071.464 kb on - strand at 3071.464 kb on - strand at 3071.464 kb on - strand at 3071.464 kb on - strand at 3071.466 kb on + strand at 3071.466 kb on + strand at 3071.466 kb on + strand at 3071.466 kb on + strand at 3071.466 kb on + strand at 3071.466 kb on + strand at 3071.466 kb on + strand at 3071.467 kb on - strand at 3071.467 kb on - strand at 3071.467 kb on - strand at 3071.467 kb on - strand at 3071.467 kb on - strand at 3071.467 kb on - strand at 3071.467 kb on - strand at 3071.467 kb on - strand at 3071.485 kb on - strand, within Pf1N1B4_2732 at 3071.502 kb on + strand, within Pf1N1B4_2732 at 3071.502 kb on + strand, within Pf1N1B4_2732 at 3071.503 kb on - strand, within Pf1N1B4_2732 at 3071.528 kb on + strand, within Pf1N1B4_2732 at 3071.528 kb on + strand, within Pf1N1B4_2732 at 3071.529 kb on - strand, within Pf1N1B4_2732 at 3071.529 kb on - strand, within Pf1N1B4_2732 at 3071.553 kb on + strand, within Pf1N1B4_2732 at 3071.553 kb on + strand, within Pf1N1B4_2732 at 3071.553 kb on + strand at 3071.554 kb on - strand, within Pf1N1B4_2732 at 3071.554 kb on - strand at 3071.554 kb on - strand, within Pf1N1B4_2732 at 3071.554 kb on - strand, within Pf1N1B4_2732 at 3071.554 kb on - strand, within Pf1N1B4_2732 at 3071.554 kb on - strand, within Pf1N1B4_2732 at 3071.554 kb on - strand at 3071.554 kb on - strand, within Pf1N1B4_2732 at 3071.554 kb on - strand, within Pf1N1B4_2732 at 3071.554 kb on - strand, within Pf1N1B4_2732 at 3071.643 kb on + strand, within Pf1N1B4_2732 at 3071.643 kb on + strand, within Pf1N1B4_2732 at 3071.643 kb on + strand, within Pf1N1B4_2732 at 3071.643 kb on + strand, within Pf1N1B4_2732 at 3071.644 kb on - strand, within Pf1N1B4_2732 at 3071.644 kb on - strand, within Pf1N1B4_2732 at 3071.644 kb on - strand, within Pf1N1B4_2732 at 3071.644 kb on - strand, within Pf1N1B4_2732 at 3071.790 kb on - strand, within Pf1N1B4_2732 at 3071.936 kb on + strand at 3071.936 kb on + strand at 3071.936 kb on + strand at 3071.937 kb on - strand at 3072.081 kb on + strand, within Pf1N1B4_2733 at 3072.082 kb on - strand at 3072.082 kb on - strand, within Pf1N1B4_2733 at 3072.082 kb on - strand, within Pf1N1B4_2733 at 3072.223 kb on + strand, within Pf1N1B4_2733 at 3072.224 kb on - strand, within Pf1N1B4_2733
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 3,069,597 + -0.0 3,069,598 - -0.0 3,069,598 - -0.0 3,069,599 + -1.2 3,069,599 + -0.0 3,069,599 + -0.0 3,069,600 - -0.0 3,069,600 - +0.3 3,069,600 - -0.0 3,069,605 + -0.0 3,069,656 + -0.0 3,069,816 - Pf1N1B4_2730 0.21 -2.1 3,069,962 + Pf1N1B4_2730 0.42 -0.0 3,070,010 + -0.0 3,070,010 + Pf1N1B4_2730 0.49 -0.0 3,070,010 + Pf1N1B4_2730 0.49 -2.9 3,070,010 + Pf1N1B4_2730 0.49 -3.6 3,070,010 + Pf1N1B4_2730 0.49 -3.7 3,070,011 - Pf1N1B4_2730 0.50 -0.0 3,070,163 + Pf1N1B4_2730 0.72 -1.6 3,070,163 + Pf1N1B4_2730 0.72 -0.0 3,070,163 + Pf1N1B4_2730 0.72 -2.8 3,070,163 + Pf1N1B4_2730 0.72 -1.6 3,070,163 + Pf1N1B4_2730 0.72 -0.0 3,070,163 + Pf1N1B4_2730 0.72 -0.0 3,070,164 - Pf1N1B4_2730 0.72 -0.0 3,070,164 - Pf1N1B4_2730 0.72 -0.0 3,070,164 - Pf1N1B4_2730 0.72 -4.3 3,070,164 - Pf1N1B4_2730 0.72 -0.0 3,070,164 - Pf1N1B4_2730 0.72 +0.1 3,070,164 - Pf1N1B4_2730 0.72 -0.0 3,070,165 + Pf1N1B4_2730 0.72 -0.0 3,070,165 + Pf1N1B4_2730 0.72 -0.0 3,070,192 + Pf1N1B4_2730 0.76 -3.1 3,070,192 + Pf1N1B4_2730 0.76 -0.0 3,070,193 - Pf1N1B4_2730 0.77 -0.0 3,070,193 - Pf1N1B4_2730 0.77 -0.0 3,070,193 - Pf1N1B4_2730 0.77 -0.0 3,070,193 - Pf1N1B4_2730 0.77 -0.0 3,070,316 + -0.0 3,070,316 + -0.0 3,070,316 + -0.0 3,070,317 - -2.3 3,070,317 - -0.0 3,070,317 - -0.0 3,070,317 - -0.0 3,070,317 - -0.0 3,070,317 - -0.0 3,070,333 + -0.0 3,070,334 - -4.6 3,070,334 - -0.0 3,070,334 - -0.0 3,070,334 - -0.0 3,070,334 - -0.0 3,070,334 - -1.5 3,070,334 - -0.0 3,070,334 - -2.9 3,070,334 - -0.0 3,070,388 + -0.0 3,070,418 + -1.5 3,070,418 + -0.0 3,070,418 + -1.0 3,070,418 + -1.4 3,070,419 - -0.0 3,070,419 - -0.0 3,070,419 - -0.0 3,070,452 - -0.0 3,070,503 + +0.2 3,070,517 + -0.0 3,070,517 + -0.0 3,070,517 + -0.0 3,070,517 + -4.2 3,070,517 + -0.0 3,070,518 - -3.3 3,070,518 - -0.0 3,070,665 - Pf1N1B4_2731 0.18 -0.0 3,070,665 - Pf1N1B4_2731 0.18 -1.8 3,070,973 + Pf1N1B4_2731 0.59 -0.7 3,070,974 - Pf1N1B4_2731 0.59 -0.0 3,070,974 - Pf1N1B4_2731 0.59 -1.8 3,070,974 - Pf1N1B4_2731 0.59 -0.0 3,070,974 - Pf1N1B4_2731 0.59 -0.0 3,071,072 - Pf1N1B4_2731 0.72 -0.0 3,071,191 + Pf1N1B4_2731 0.88 -0.0 3,071,192 - Pf1N1B4_2731 0.88 -1.6 3,071,192 - Pf1N1B4_2731 0.88 -0.0 3,071,192 - Pf1N1B4_2731 0.88 -0.0 3,071,228 + -0.0 3,071,229 - -0.0 3,071,229 - -0.0 3,071,229 - -3.2 3,071,230 + -1.8 3,071,355 + -0.0 3,071,355 + -5.2 3,071,355 + -0.0 3,071,356 - -0.0 3,071,420 + -0.0 3,071,420 + -1.9 3,071,420 + -0.0 3,071,420 + -1.4 3,071,420 + -1.8 3,071,420 + -0.0 3,071,420 + -0.1 3,071,420 + -0.0 3,071,420 + -4.0 3,071,421 - -1.2 3,071,421 - -0.0 3,071,421 - -0.0 3,071,421 - -0.0 3,071,421 - -0.4 3,071,421 - -0.0 3,071,421 - -0.0 3,071,421 - -0.0 3,071,463 + -0.0 3,071,463 + -0.0 3,071,464 - -0.0 3,071,464 - -0.0 3,071,464 - +0.3 3,071,464 - -0.0 3,071,464 - -0.0 3,071,464 - -2.3 3,071,466 + -0.0 3,071,466 + -4.7 3,071,466 + -0.0 3,071,466 + -0.0 3,071,466 + -0.0 3,071,466 + -2.3 3,071,466 + -0.0 3,071,467 - -0.0 3,071,467 - -4.7 3,071,467 - -0.0 3,071,467 - -0.0 3,071,467 - -0.0 3,071,467 - -0.0 3,071,467 - -1.6 3,071,467 - -1.7 3,071,485 - Pf1N1B4_2732 0.13 -2.6 3,071,502 + Pf1N1B4_2732 0.16 -2.0 3,071,502 + Pf1N1B4_2732 0.16 -0.0 3,071,503 - Pf1N1B4_2732 0.16 -0.0 3,071,528 + Pf1N1B4_2732 0.22 -0.0 3,071,528 + Pf1N1B4_2732 0.22 -0.0 3,071,529 - Pf1N1B4_2732 0.22 -1.3 3,071,529 - Pf1N1B4_2732 0.22 -0.0 3,071,553 + Pf1N1B4_2732 0.27 -1.9 3,071,553 + Pf1N1B4_2732 0.27 -0.0 3,071,553 + -0.0 3,071,554 - Pf1N1B4_2732 0.27 -0.0 3,071,554 - -0.0 3,071,554 - Pf1N1B4_2732 0.27 -0.9 3,071,554 - Pf1N1B4_2732 0.27 -0.0 3,071,554 - Pf1N1B4_2732 0.27 -0.0 3,071,554 - Pf1N1B4_2732 0.27 -3.4 3,071,554 - -0.0 3,071,554 - Pf1N1B4_2732 0.27 -0.0 3,071,554 - Pf1N1B4_2732 0.27 -0.0 3,071,554 - Pf1N1B4_2732 0.27 -0.0 3,071,643 + Pf1N1B4_2732 0.46 -0.5 3,071,643 + Pf1N1B4_2732 0.46 -0.0 3,071,643 + Pf1N1B4_2732 0.46 -0.0 3,071,643 + Pf1N1B4_2732 0.46 -5.7 3,071,644 - Pf1N1B4_2732 0.46 -3.8 3,071,644 - Pf1N1B4_2732 0.46 -0.0 3,071,644 - Pf1N1B4_2732 0.46 -1.4 3,071,644 - Pf1N1B4_2732 0.46 -2.5 3,071,790 - Pf1N1B4_2732 0.76 -2.0 3,071,936 + -0.0 3,071,936 + -0.0 3,071,936 + -0.0 3,071,937 - -2.9 3,072,081 + Pf1N1B4_2733 0.25 -0.0 3,072,082 - -2.0 3,072,082 - Pf1N1B4_2733 0.25 -0.0 3,072,082 - Pf1N1B4_2733 0.25 -3.4 3,072,223 + Pf1N1B4_2733 0.49 -2.3 3,072,224 - Pf1N1B4_2733 0.49 -0.0
Or see this region's nucleotide sequence