Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_262 and Pf1N1B4_263 are separated by 201 nucleotides Pf1N1B4_263 and Pf1N1B4_264 are separated by 103 nucleotides
Pf1N1B4_262 - Probable protease htpX homolog (EC 3.4.24.-), at 322,107 to 322,994
_262
Pf1N1B4_263 - Thiopurine S-methyltransferase (EC 2.1.1.67), at 323,196 to 323,852
_263
Pf1N1B4_264 - FIG074102: hypothetical protein, at 323,956 to 324,723
_264
Position (kb)
323
324 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 322.434 kb on + strand, within Pf1N1B4_262 at 322.434 kb on + strand, within Pf1N1B4_262 at 322.434 kb on + strand, within Pf1N1B4_262 at 322.434 kb on + strand, within Pf1N1B4_262 at 322.434 kb on + strand, within Pf1N1B4_262 at 322.434 kb on + strand, within Pf1N1B4_262 at 322.435 kb on - strand, within Pf1N1B4_262 at 322.435 kb on - strand, within Pf1N1B4_262 at 322.435 kb on - strand, within Pf1N1B4_262 at 322.435 kb on - strand, within Pf1N1B4_262 at 322.435 kb on - strand, within Pf1N1B4_262 at 322.435 kb on - strand, within Pf1N1B4_262 at 322.435 kb on - strand, within Pf1N1B4_262 at 322.435 kb on - strand, within Pf1N1B4_262 at 322.435 kb on - strand, within Pf1N1B4_262 at 322.644 kb on - strand, within Pf1N1B4_262 at 322.688 kb on + strand, within Pf1N1B4_262 at 322.688 kb on + strand, within Pf1N1B4_262 at 322.689 kb on - strand, within Pf1N1B4_262 at 322.695 kb on + strand, within Pf1N1B4_262 at 322.695 kb on + strand, within Pf1N1B4_262 at 322.695 kb on + strand, within Pf1N1B4_262 at 322.695 kb on + strand, within Pf1N1B4_262 at 322.696 kb on - strand, within Pf1N1B4_262 at 322.696 kb on - strand, within Pf1N1B4_262 at 322.696 kb on - strand, within Pf1N1B4_262 at 322.696 kb on - strand, within Pf1N1B4_262 at 322.696 kb on - strand, within Pf1N1B4_262 at 322.696 kb on - strand, within Pf1N1B4_262 at 322.696 kb on - strand, within Pf1N1B4_262 at 322.696 kb on - strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.698 kb on + strand, within Pf1N1B4_262 at 322.699 kb on - strand, within Pf1N1B4_262 at 322.699 kb on - strand, within Pf1N1B4_262 at 322.699 kb on - strand, within Pf1N1B4_262 at 322.699 kb on - strand, within Pf1N1B4_262 at 322.699 kb on - strand, within Pf1N1B4_262 at 322.699 kb on - strand, within Pf1N1B4_262 at 322.699 kb on - strand, within Pf1N1B4_262 at 322.699 kb on - strand, within Pf1N1B4_262 at 322.699 kb on - strand, within Pf1N1B4_262 at 322.699 kb on - strand, within Pf1N1B4_262 at 322.699 kb on - strand, within Pf1N1B4_262 at 322.699 kb on - strand, within Pf1N1B4_262 at 322.699 kb on - strand, within Pf1N1B4_262 at 322.699 kb on - strand, within Pf1N1B4_262 at 322.699 kb on - strand, within Pf1N1B4_262 at 322.699 kb on - strand, within Pf1N1B4_262 at 322.699 kb on - strand, within Pf1N1B4_262 at 322.699 kb on - strand, within Pf1N1B4_262 at 322.699 kb on - strand, within Pf1N1B4_262 at 322.699 kb on - strand, within Pf1N1B4_262 at 322.699 kb on - strand, within Pf1N1B4_262 at 322.699 kb on - strand, within Pf1N1B4_262 at 322.699 kb on - strand, within Pf1N1B4_262 at 322.699 kb on - strand, within Pf1N1B4_262 at 322.699 kb on - strand, within Pf1N1B4_262 at 322.702 kb on + strand, within Pf1N1B4_262 at 322.702 kb on + strand, within Pf1N1B4_262 at 322.703 kb on - strand, within Pf1N1B4_262 at 322.703 kb on - strand, within Pf1N1B4_262 at 322.703 kb on - strand, within Pf1N1B4_262 at 322.703 kb on - strand, within Pf1N1B4_262 at 322.934 kb on + strand at 322.934 kb on + strand at 322.934 kb on + strand at 322.934 kb on + strand at 322.934 kb on + strand at 322.934 kb on + strand at 322.934 kb on + strand at 322.935 kb on - strand at 322.935 kb on - strand at 322.935 kb on - strand at 323.057 kb on + strand at 323.103 kb on - strand at 323.127 kb on + strand at 323.150 kb on - strand at 323.181 kb on - strand at 323.193 kb on + strand at 323.194 kb on - strand at 323.194 kb on - strand at 323.194 kb on - strand at 323.194 kb on - strand at 323.194 kb on - strand at 323.194 kb on - strand at 323.194 kb on - strand at 323.194 kb on - strand at 323.194 kb on - strand at 323.194 kb on - strand at 323.194 kb on - strand at 323.194 kb on - strand at 323.198 kb on - strand at 323.380 kb on + strand, within Pf1N1B4_263 at 323.390 kb on + strand, within Pf1N1B4_263 at 323.444 kb on - strand, within Pf1N1B4_263 at 323.492 kb on - strand, within Pf1N1B4_263 at 323.492 kb on - strand, within Pf1N1B4_263 at 323.852 kb on + strand at 323.852 kb on + strand at 323.898 kb on - strand at 323.937 kb on + strand at 323.937 kb on + strand at 323.937 kb on + strand at 323.937 kb on + strand at 323.971 kb on + strand at 323.971 kb on + strand at 323.971 kb on + strand at 323.971 kb on + strand at 323.971 kb on + strand at 323.971 kb on + strand at 323.971 kb on + strand at 323.971 kb on + strand at 323.971 kb on + strand at 323.972 kb on - strand at 323.972 kb on - strand at 323.972 kb on - strand at 323.972 kb on - strand at 323.972 kb on - strand at 323.972 kb on - strand at 323.972 kb on - strand at 323.972 kb on - strand at 323.973 kb on + strand at 323.973 kb on + strand at 323.973 kb on + strand at 323.973 kb on + strand at 323.973 kb on + strand at 323.973 kb on + strand at 323.973 kb on + strand at 323.973 kb on + strand at 323.973 kb on + strand at 323.973 kb on + strand at 323.974 kb on - strand at 323.974 kb on - strand at 323.974 kb on - strand at 323.974 kb on - strand at 323.974 kb on - strand at 323.974 kb on - strand at 323.974 kb on - strand at 323.974 kb on - strand at 323.974 kb on - strand at 323.974 kb on - strand at 323.974 kb on - strand at 323.974 kb on - strand at 323.997 kb on - strand at 323.997 kb on - strand at 324.074 kb on + strand, within Pf1N1B4_264 at 324.074 kb on + strand, within Pf1N1B4_264 at 324.181 kb on + strand, within Pf1N1B4_264 at 324.310 kb on + strand, within Pf1N1B4_264 at 324.311 kb on - strand, within Pf1N1B4_264 at 324.585 kb on + strand, within Pf1N1B4_264 at 324.585 kb on + strand, within Pf1N1B4_264 at 324.585 kb on + strand, within Pf1N1B4_264 at 324.585 kb on + strand, within Pf1N1B4_264 at 324.586 kb on - strand, within Pf1N1B4_264 at 324.586 kb on - strand, within Pf1N1B4_264 at 324.586 kb on - strand, within Pf1N1B4_264 at 324.586 kb on - strand at 324.631 kb on + strand, within Pf1N1B4_264 at 324.632 kb on - strand, within Pf1N1B4_264 at 324.632 kb on - strand, within Pf1N1B4_264 at 324.632 kb on - strand, within Pf1N1B4_264 at 324.705 kb on + strand at 324.705 kb on + strand at 324.705 kb on + strand at 324.705 kb on + strand at 324.705 kb on + strand at 324.706 kb on - strand at 324.706 kb on - strand at 324.706 kb on - strand at 324.706 kb on - strand at 324.706 kb on - strand at 324.706 kb on - strand at 324.706 kb on - strand at 324.709 kb on + strand at 324.709 kb on + strand at 324.709 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 322,434 + Pf1N1B4_262 0.37 -1.5 322,434 + Pf1N1B4_262 0.37 -1.9 322,434 + Pf1N1B4_262 0.37 -3.8 322,434 + Pf1N1B4_262 0.37 -0.1 322,434 + Pf1N1B4_262 0.37 -2.2 322,434 + Pf1N1B4_262 0.37 -2.1 322,435 - Pf1N1B4_262 0.37 -1.7 322,435 - Pf1N1B4_262 0.37 -1.6 322,435 - Pf1N1B4_262 0.37 -2.3 322,435 - Pf1N1B4_262 0.37 -0.1 322,435 - Pf1N1B4_262 0.37 -1.7 322,435 - Pf1N1B4_262 0.37 -2.1 322,435 - Pf1N1B4_262 0.37 -2.8 322,435 - Pf1N1B4_262 0.37 -0.1 322,435 - Pf1N1B4_262 0.37 -4.7 322,644 - Pf1N1B4_262 0.60 -4.1 322,688 + Pf1N1B4_262 0.65 -6.0 322,688 + Pf1N1B4_262 0.65 -2.7 322,689 - Pf1N1B4_262 0.66 -0.1 322,695 + Pf1N1B4_262 0.66 -1.0 322,695 + Pf1N1B4_262 0.66 -1.8 322,695 + Pf1N1B4_262 0.66 -1.8 322,695 + Pf1N1B4_262 0.66 -2.8 322,696 - Pf1N1B4_262 0.66 -0.1 322,696 - Pf1N1B4_262 0.66 -3.6 322,696 - Pf1N1B4_262 0.66 -0.1 322,696 - Pf1N1B4_262 0.66 -2.5 322,696 - Pf1N1B4_262 0.66 -2.3 322,696 - Pf1N1B4_262 0.66 -3.3 322,696 - Pf1N1B4_262 0.66 -0.1 322,696 - Pf1N1B4_262 0.66 -3.7 322,698 + Pf1N1B4_262 0.67 -1.7 322,698 + Pf1N1B4_262 0.67 -0.1 322,698 + Pf1N1B4_262 0.67 -0.1 322,698 + Pf1N1B4_262 0.67 -2.9 322,698 + Pf1N1B4_262 0.67 -0.1 322,698 + Pf1N1B4_262 0.67 -5.5 322,698 + Pf1N1B4_262 0.67 -1.4 322,698 + Pf1N1B4_262 0.67 -4.5 322,698 + Pf1N1B4_262 0.67 -2.4 322,698 + Pf1N1B4_262 0.67 -1.6 322,698 + Pf1N1B4_262 0.67 -0.1 322,698 + Pf1N1B4_262 0.67 -1.4 322,698 + Pf1N1B4_262 0.67 -1.4 322,698 + Pf1N1B4_262 0.67 -0.1 322,698 + Pf1N1B4_262 0.67 -3.5 322,698 + Pf1N1B4_262 0.67 -4.0 322,698 + Pf1N1B4_262 0.67 -2.4 322,698 + Pf1N1B4_262 0.67 -1.3 322,698 + Pf1N1B4_262 0.67 -2.4 322,698 + Pf1N1B4_262 0.67 -5.6 322,698 + Pf1N1B4_262 0.67 -5.8 322,698 + Pf1N1B4_262 0.67 -5.9 322,698 + Pf1N1B4_262 0.67 -2.4 322,698 + Pf1N1B4_262 0.67 -0.1 322,698 + Pf1N1B4_262 0.67 -4.1 322,698 + Pf1N1B4_262 0.67 -2.9 322,698 + Pf1N1B4_262 0.67 -0.1 322,698 + Pf1N1B4_262 0.67 -2.6 322,698 + Pf1N1B4_262 0.67 -1.8 322,698 + Pf1N1B4_262 0.67 -1.7 322,698 + Pf1N1B4_262 0.67 -1.4 322,698 + Pf1N1B4_262 0.67 -4.0 322,698 + Pf1N1B4_262 0.67 -1.3 322,698 + Pf1N1B4_262 0.67 -2.4 322,698 + Pf1N1B4_262 0.67 -4.8 322,698 + Pf1N1B4_262 0.67 -4.9 322,698 + Pf1N1B4_262 0.67 -0.1 322,699 - Pf1N1B4_262 0.67 -3.4 322,699 - Pf1N1B4_262 0.67 -2.4 322,699 - Pf1N1B4_262 0.67 -0.1 322,699 - Pf1N1B4_262 0.67 -0.1 322,699 - Pf1N1B4_262 0.67 -0.1 322,699 - Pf1N1B4_262 0.67 -4.5 322,699 - Pf1N1B4_262 0.67 -0.5 322,699 - Pf1N1B4_262 0.67 -0.1 322,699 - Pf1N1B4_262 0.67 -5.2 322,699 - Pf1N1B4_262 0.67 -0.1 322,699 - Pf1N1B4_262 0.67 -1.3 322,699 - Pf1N1B4_262 0.67 -3.7 322,699 - Pf1N1B4_262 0.67 -0.1 322,699 - Pf1N1B4_262 0.67 -4.0 322,699 - Pf1N1B4_262 0.67 -1.6 322,699 - Pf1N1B4_262 0.67 -3.9 322,699 - Pf1N1B4_262 0.67 -1.6 322,699 - Pf1N1B4_262 0.67 -1.2 322,699 - Pf1N1B4_262 0.67 -3.1 322,699 - Pf1N1B4_262 0.67 -1.7 322,699 - Pf1N1B4_262 0.67 -1.4 322,699 - Pf1N1B4_262 0.67 -0.1 322,699 - Pf1N1B4_262 0.67 -5.7 322,699 - Pf1N1B4_262 0.67 -1.2 322,699 - Pf1N1B4_262 0.67 -0.1 322,702 + Pf1N1B4_262 0.67 -0.6 322,702 + Pf1N1B4_262 0.67 -1.6 322,703 - Pf1N1B4_262 0.67 -0.6 322,703 - Pf1N1B4_262 0.67 +1.1 322,703 - Pf1N1B4_262 0.67 -1.2 322,703 - Pf1N1B4_262 0.67 -0.1 322,934 + -2.3 322,934 + -3.5 322,934 + -2.6 322,934 + -0.1 322,934 + -1.1 322,934 + -4.0 322,934 + -0.1 322,935 - -0.1 322,935 - +0.9 322,935 - -0.1 323,057 + -4.3 323,103 - -0.1 323,127 + -0.1 323,150 - -1.3 323,181 - -0.1 323,193 + -2.7 323,194 - -0.1 323,194 - -0.1 323,194 - -0.1 323,194 - -1.7 323,194 - -0.1 323,194 - -0.1 323,194 - -1.5 323,194 - -2.4 323,194 - -0.1 323,194 - -2.5 323,194 - +0.2 323,194 - -0.1 323,198 - -0.1 323,380 + Pf1N1B4_263 0.28 -2.0 323,390 + Pf1N1B4_263 0.30 -0.1 323,444 - Pf1N1B4_263 0.38 -3.2 323,492 - Pf1N1B4_263 0.45 -0.1 323,492 - Pf1N1B4_263 0.45 -0.1 323,852 + -1.4 323,852 + -0.1 323,898 - -0.1 323,937 + -0.1 323,937 + -0.1 323,937 + -0.1 323,937 + -0.1 323,971 + -3.1 323,971 + -0.1 323,971 + -0.1 323,971 + -0.1 323,971 + -1.6 323,971 + -0.1 323,971 + -0.1 323,971 + -0.1 323,971 + -1.7 323,972 - -0.1 323,972 - -3.3 323,972 - -1.2 323,972 - -0.1 323,972 - -0.1 323,972 - -2.7 323,972 - -2.7 323,972 - -0.1 323,973 + -0.1 323,973 + -1.5 323,973 + -2.5 323,973 + -1.3 323,973 + -0.1 323,973 + -0.1 323,973 + -4.6 323,973 + -0.1 323,973 + -0.1 323,973 + -3.0 323,974 - -0.1 323,974 - -0.1 323,974 - -1.1 323,974 - -3.3 323,974 - -0.1 323,974 - -3.6 323,974 - -0.1 323,974 - -0.1 323,974 - -1.7 323,974 - -0.1 323,974 - -0.1 323,974 - +0.5 323,997 - -0.1 323,997 - -5.1 324,074 + Pf1N1B4_264 0.15 -0.1 324,074 + Pf1N1B4_264 0.15 -0.1 324,181 + Pf1N1B4_264 0.29 -1.7 324,310 + Pf1N1B4_264 0.46 -0.1 324,311 - Pf1N1B4_264 0.46 -0.1 324,585 + Pf1N1B4_264 0.82 -0.1 324,585 + Pf1N1B4_264 0.82 -2.3 324,585 + Pf1N1B4_264 0.82 -0.9 324,585 + Pf1N1B4_264 0.82 -0.1 324,586 - Pf1N1B4_264 0.82 -1.3 324,586 - Pf1N1B4_264 0.82 -0.1 324,586 - Pf1N1B4_264 0.82 -0.9 324,586 - -0.1 324,631 + Pf1N1B4_264 0.88 -0.1 324,632 - Pf1N1B4_264 0.88 -1.7 324,632 - Pf1N1B4_264 0.88 -0.1 324,632 - Pf1N1B4_264 0.88 -1.0 324,705 + -2.2 324,705 + -0.1 324,705 + -0.1 324,705 + -2.3 324,705 + -1.8 324,706 - -0.1 324,706 - -0.1 324,706 - -0.4 324,706 - -0.1 324,706 - -1.6 324,706 - -0.9 324,706 - -0.7 324,709 + -0.1 324,709 + -0.1 324,709 + -0.1
Or see this region's nucleotide sequence