Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2460

Experiment: Nitrate reduction

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2457 and Pf1N1B4_2458 are separated by 129 nucleotidesPf1N1B4_2458 and Pf1N1B4_2459 overlap by 17 nucleotidesPf1N1B4_2459 and Pf1N1B4_2460 are separated by 6 nucleotidesPf1N1B4_2460 and Pf1N1B4_2461 are separated by 205 nucleotides Pf1N1B4_2457 - Sarcosine oxidase alpha subunit (EC 1.5.3.1), at 2,786,534 to 2,789,551 _2457 Pf1N1B4_2458 - Sarcosine oxidase gamma subunit (EC 1.5.3.1), at 2,789,681 to 2,790,313 _2458 Pf1N1B4_2459 - hypothetical protein, at 2,790,297 to 2,790,440 _2459 Pf1N1B4_2460 - Formyltetrahydrofolate deformylase (EC 3.5.1.10), at 2,790,447 to 2,791,304 _2460 Pf1N1B4_2461 - Threonine dehydrogenase and related Zn-dependent dehydrogenases, at 2,791,510 to 2,792,709 _2461 Position (kb) 2790 2791 2792Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 2789.549 kb on + strandat 2789.549 kb on + strandat 2789.550 kb on - strandat 2789.550 kb on - strandat 2789.680 kb on + strandat 2789.681 kb on - strandat 2790.096 kb on + strand, within Pf1N1B4_2458at 2790.097 kb on - strand, within Pf1N1B4_2458at 2790.125 kb on + strand, within Pf1N1B4_2458at 2790.125 kb on + strand, within Pf1N1B4_2458at 2790.125 kb on + strand, within Pf1N1B4_2458at 2790.126 kb on - strand, within Pf1N1B4_2458at 2790.126 kb on - strand, within Pf1N1B4_2458at 2790.126 kb on - strand, within Pf1N1B4_2458at 2790.142 kb on + strand, within Pf1N1B4_2458at 2790.298 kb on + strandat 2790.312 kb on - strandat 2790.443 kb on + strandat 2790.444 kb on - strandat 2790.444 kb on - strandat 2790.446 kb on + strandat 2790.446 kb on + strandat 2790.446 kb on + strandat 2790.546 kb on + strand, within Pf1N1B4_2460at 2790.547 kb on - strand, within Pf1N1B4_2460at 2790.547 kb on - strand, within Pf1N1B4_2460at 2790.722 kb on - strand, within Pf1N1B4_2460at 2790.868 kb on - strand, within Pf1N1B4_2460at 2790.966 kb on + strand, within Pf1N1B4_2460at 2790.966 kb on + strand, within Pf1N1B4_2460at 2790.966 kb on + strand, within Pf1N1B4_2460at 2790.966 kb on + strand, within Pf1N1B4_2460at 2790.967 kb on - strand, within Pf1N1B4_2460at 2791.077 kb on + strand, within Pf1N1B4_2460at 2791.078 kb on - strand, within Pf1N1B4_2460at 2791.116 kb on + strand, within Pf1N1B4_2460at 2791.116 kb on + strand, within Pf1N1B4_2460at 2791.116 kb on + strand, within Pf1N1B4_2460at 2791.116 kb on + strand, within Pf1N1B4_2460at 2791.117 kb on - strand, within Pf1N1B4_2460at 2791.117 kb on - strand, within Pf1N1B4_2460at 2791.117 kb on - strand, within Pf1N1B4_2460at 2791.117 kb on - strand, within Pf1N1B4_2460at 2791.178 kb on + strand, within Pf1N1B4_2460at 2791.179 kb on - strand, within Pf1N1B4_2460at 2791.179 kb on - strand, within Pf1N1B4_2460at 2791.179 kb on - strand, within Pf1N1B4_2460at 2791.179 kb on - strand, within Pf1N1B4_2460at 2791.179 kb on - strand, within Pf1N1B4_2460at 2791.179 kb on - strand, within Pf1N1B4_2460at 2791.302 kb on + strandat 2791.303 kb on - strandat 2791.363 kb on + strandat 2791.364 kb on - strandat 2791.364 kb on - strandat 2791.364 kb on - strandat 2791.482 kb on + strandat 2791.482 kb on + strandat 2791.483 kb on - strandat 2791.483 kb on - strandat 2791.483 kb on - strandat 2791.486 kb on + strandat 2791.486 kb on + strandat 2791.486 kb on + strandat 2791.486 kb on + strandat 2791.487 kb on - strandat 2791.487 kb on - strandat 2791.487 kb on - strandat 2791.487 kb on - strandat 2791.559 kb on + strandat 2791.680 kb on + strand, within Pf1N1B4_2461at 2791.681 kb on - strand, within Pf1N1B4_2461at 2791.833 kb on + strand, within Pf1N1B4_2461at 2791.833 kb on + strand, within Pf1N1B4_2461at 2791.833 kb on + strand, within Pf1N1B4_2461at 2791.833 kb on + strand, within Pf1N1B4_2461at 2791.834 kb on - strand, within Pf1N1B4_2461at 2791.834 kb on - strand, within Pf1N1B4_2461at 2791.834 kb on - strand, within Pf1N1B4_2461at 2791.834 kb on - strand, within Pf1N1B4_2461at 2791.834 kb on - strand, within Pf1N1B4_2461at 2791.834 kb on - strand, within Pf1N1B4_2461at 2791.834 kb on - strand, within Pf1N1B4_2461at 2791.879 kb on + strand, within Pf1N1B4_2461at 2791.880 kb on - strand, within Pf1N1B4_2461at 2791.880 kb on - strand, within Pf1N1B4_2461at 2791.880 kb on - strandat 2791.880 kb on - strand, within Pf1N1B4_2461at 2791.925 kb on - strand, within Pf1N1B4_2461at 2791.939 kb on + strand, within Pf1N1B4_2461at 2791.939 kb on + strand, within Pf1N1B4_2461at 2792.080 kb on + strand, within Pf1N1B4_2461at 2792.080 kb on + strand, within Pf1N1B4_2461at 2792.081 kb on - strandat 2792.298 kb on - strand, within Pf1N1B4_2461

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction
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2,789,549 + -0.0
2,789,549 + -0.0
2,789,550 - -0.0
2,789,550 - -0.0
2,789,680 + -0.0
2,789,681 - -0.0
2,790,096 + Pf1N1B4_2458 0.66 -0.0
2,790,097 - Pf1N1B4_2458 0.66 -0.0
2,790,125 + Pf1N1B4_2458 0.70 -0.0
2,790,125 + Pf1N1B4_2458 0.70 -0.0
2,790,125 + Pf1N1B4_2458 0.70 -1.9
2,790,126 - Pf1N1B4_2458 0.70 -0.0
2,790,126 - Pf1N1B4_2458 0.70 -0.0
2,790,126 - Pf1N1B4_2458 0.70 -0.0
2,790,142 + Pf1N1B4_2458 0.73 -0.1
2,790,298 + -0.0
2,790,312 - -1.7
2,790,443 + -0.0
2,790,444 - -0.0
2,790,444 - -0.9
2,790,446 + -0.0
2,790,446 + -3.5
2,790,446 + -0.0
2,790,546 + Pf1N1B4_2460 0.12 -3.0
2,790,547 - Pf1N1B4_2460 0.12 -0.0
2,790,547 - Pf1N1B4_2460 0.12 -0.8
2,790,722 - Pf1N1B4_2460 0.32 -0.0
2,790,868 - Pf1N1B4_2460 0.49 -0.0
2,790,966 + Pf1N1B4_2460 0.60 -1.8
2,790,966 + Pf1N1B4_2460 0.60 +0.1
2,790,966 + Pf1N1B4_2460 0.60 -2.4
2,790,966 + Pf1N1B4_2460 0.60 -0.0
2,790,967 - Pf1N1B4_2460 0.61 -0.0
2,791,077 + Pf1N1B4_2460 0.73 -0.0
2,791,078 - Pf1N1B4_2460 0.74 -1.3
2,791,116 + Pf1N1B4_2460 0.78 -0.0
2,791,116 + Pf1N1B4_2460 0.78 -2.2
2,791,116 + Pf1N1B4_2460 0.78 -4.1
2,791,116 + Pf1N1B4_2460 0.78 -1.8
2,791,117 - Pf1N1B4_2460 0.78 -0.0
2,791,117 - Pf1N1B4_2460 0.78 -0.0
2,791,117 - Pf1N1B4_2460 0.78 -0.0
2,791,117 - Pf1N1B4_2460 0.78 -2.6
2,791,178 + Pf1N1B4_2460 0.85 -1.3
2,791,179 - Pf1N1B4_2460 0.85 -0.0
2,791,179 - Pf1N1B4_2460 0.85 -0.0
2,791,179 - Pf1N1B4_2460 0.85 -2.2
2,791,179 - Pf1N1B4_2460 0.85 -0.0
2,791,179 - Pf1N1B4_2460 0.85 -0.0
2,791,179 - Pf1N1B4_2460 0.85 -1.1
2,791,302 + -0.8
2,791,303 - -0.0
2,791,363 + -0.0
2,791,364 - -0.0
2,791,364 - -0.0
2,791,364 - -1.0
2,791,482 + -0.0
2,791,482 + -1.4
2,791,483 - -0.0
2,791,483 - -1.5
2,791,483 - -1.1
2,791,486 + -0.0
2,791,486 + -0.0
2,791,486 + -0.0
2,791,486 + -0.0
2,791,487 - -0.0
2,791,487 - +1.0
2,791,487 - -0.0
2,791,487 - -0.5
2,791,559 + -2.2
2,791,680 + Pf1N1B4_2461 0.14 -0.0
2,791,681 - Pf1N1B4_2461 0.14 -0.0
2,791,833 + Pf1N1B4_2461 0.27 -0.0
2,791,833 + Pf1N1B4_2461 0.27 -0.4
2,791,833 + Pf1N1B4_2461 0.27 -0.2
2,791,833 + Pf1N1B4_2461 0.27 -0.0
2,791,834 - Pf1N1B4_2461 0.27 -0.0
2,791,834 - Pf1N1B4_2461 0.27 -1.8
2,791,834 - Pf1N1B4_2461 0.27 -0.0
2,791,834 - Pf1N1B4_2461 0.27 -1.4
2,791,834 - Pf1N1B4_2461 0.27 -0.0
2,791,834 - Pf1N1B4_2461 0.27 -0.0
2,791,834 - Pf1N1B4_2461 0.27 -0.0
2,791,879 + Pf1N1B4_2461 0.31 -0.0
2,791,880 - Pf1N1B4_2461 0.31 -0.0
2,791,880 - Pf1N1B4_2461 0.31 -0.0
2,791,880 - -0.0
2,791,880 - Pf1N1B4_2461 0.31 -0.0
2,791,925 - Pf1N1B4_2461 0.35 -1.4
2,791,939 + Pf1N1B4_2461 0.36 -0.0
2,791,939 + Pf1N1B4_2461 0.36 -5.7
2,792,080 + Pf1N1B4_2461 0.47 -0.0
2,792,080 + Pf1N1B4_2461 0.47 -0.0
2,792,081 - -0.0
2,792,298 - Pf1N1B4_2461 0.66 -0.0

Or see this region's nucleotide sequence