Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2441 and Pf1N1B4_2442 are separated by 15 nucleotides Pf1N1B4_2442 and Pf1N1B4_2443 are separated by 188 nucleotides
Pf1N1B4_2441 - Electron transfer flavoprotein, alpha subunit, at 2,766,106 to 2,767,326
_2441
Pf1N1B4_2442 - Electron transfer flavoprotein, beta subunit, at 2,767,342 to 2,768,112
_2442
Pf1N1B4_2443 - acetyltransferase, GNAT family, at 2,768,301 to 2,768,867
_2443
Position (kb)
2767
2768
2769 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2766.373 kb on + strand, within Pf1N1B4_2441 at 2766.373 kb on + strand, within Pf1N1B4_2441 at 2766.373 kb on + strand, within Pf1N1B4_2441 at 2766.374 kb on - strand, within Pf1N1B4_2441 at 2766.374 kb on - strand, within Pf1N1B4_2441 at 2766.374 kb on - strand, within Pf1N1B4_2441 at 2766.483 kb on - strand, within Pf1N1B4_2441 at 2766.483 kb on - strand at 2766.568 kb on + strand, within Pf1N1B4_2441 at 2767.120 kb on + strand, within Pf1N1B4_2441 at 2767.121 kb on - strand, within Pf1N1B4_2441 at 2767.286 kb on + strand at 2767.286 kb on + strand at 2767.342 kb on - strand at 2767.510 kb on + strand, within Pf1N1B4_2442 at 2767.511 kb on - strand, within Pf1N1B4_2442 at 2767.588 kb on + strand, within Pf1N1B4_2442 at 2767.588 kb on + strand, within Pf1N1B4_2442 at 2767.589 kb on - strand, within Pf1N1B4_2442 at 2767.589 kb on - strand, within Pf1N1B4_2442 at 2767.589 kb on - strand, within Pf1N1B4_2442 at 2767.790 kb on + strand at 2767.790 kb on + strand, within Pf1N1B4_2442 at 2767.790 kb on + strand, within Pf1N1B4_2442 at 2767.791 kb on - strand, within Pf1N1B4_2442 at 2767.872 kb on - strand, within Pf1N1B4_2442 at 2768.290 kb on - strand at 2768.299 kb on + strand at 2768.300 kb on - strand at 2768.388 kb on + strand, within Pf1N1B4_2443 at 2768.389 kb on - strand, within Pf1N1B4_2443 at 2768.389 kb on - strand, within Pf1N1B4_2443 at 2768.532 kb on + strand at 2768.532 kb on + strand, within Pf1N1B4_2443 at 2768.532 kb on + strand, within Pf1N1B4_2443 at 2768.532 kb on + strand, within Pf1N1B4_2443 at 2768.532 kb on + strand, within Pf1N1B4_2443 at 2768.532 kb on + strand, within Pf1N1B4_2443 at 2768.533 kb on - strand, within Pf1N1B4_2443 at 2768.533 kb on - strand, within Pf1N1B4_2443 at 2768.533 kb on - strand, within Pf1N1B4_2443 at 2768.533 kb on - strand, within Pf1N1B4_2443 at 2768.590 kb on - strand, within Pf1N1B4_2443 at 2768.619 kb on + strand, within Pf1N1B4_2443 at 2768.619 kb on + strand, within Pf1N1B4_2443 at 2768.620 kb on - strand, within Pf1N1B4_2443 at 2768.621 kb on + strand, within Pf1N1B4_2443 at 2768.621 kb on + strand at 2768.621 kb on + strand, within Pf1N1B4_2443 at 2768.621 kb on + strand, within Pf1N1B4_2443 at 2768.622 kb on - strand, within Pf1N1B4_2443 at 2768.622 kb on - strand, within Pf1N1B4_2443 at 2768.622 kb on - strand, within Pf1N1B4_2443 at 2768.686 kb on - strand, within Pf1N1B4_2443 at 2768.692 kb on - strand, within Pf1N1B4_2443 at 2768.769 kb on + strand, within Pf1N1B4_2443 at 2768.769 kb on + strand, within Pf1N1B4_2443 at 2768.847 kb on + strand at 2768.847 kb on + strand at 2768.847 kb on + strand at 2768.847 kb on + strand at 2768.847 kb on + strand at 2768.848 kb on - strand at 2768.848 kb on - strand at 2768.848 kb on - strand at 2768.848 kb on - strand at 2768.848 kb on - strand at 2768.856 kb on + strand at 2768.857 kb on - strand at 2768.866 kb on - strand at 2768.981 kb on + strand at 2768.981 kb on + strand at 2768.982 kb on - strand at 2768.982 kb on - strand at 2768.982 kb on - strand at 2768.982 kb on - strand at 2768.982 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 2,766,373 + Pf1N1B4_2441 0.22 -0.0 2,766,373 + Pf1N1B4_2441 0.22 -0.0 2,766,373 + Pf1N1B4_2441 0.22 -0.0 2,766,374 - Pf1N1B4_2441 0.22 -0.0 2,766,374 - Pf1N1B4_2441 0.22 +0.3 2,766,374 - Pf1N1B4_2441 0.22 -1.6 2,766,483 - Pf1N1B4_2441 0.31 -1.1 2,766,483 - -2.9 2,766,568 + Pf1N1B4_2441 0.38 -3.9 2,767,120 + Pf1N1B4_2441 0.83 -0.0 2,767,121 - Pf1N1B4_2441 0.83 -0.0 2,767,286 + -0.0 2,767,286 + -0.0 2,767,342 - -0.0 2,767,510 + Pf1N1B4_2442 0.22 -3.2 2,767,511 - Pf1N1B4_2442 0.22 -4.1 2,767,588 + Pf1N1B4_2442 0.32 -0.0 2,767,588 + Pf1N1B4_2442 0.32 -0.0 2,767,589 - Pf1N1B4_2442 0.32 -0.0 2,767,589 - Pf1N1B4_2442 0.32 -2.5 2,767,589 - Pf1N1B4_2442 0.32 -0.0 2,767,790 + +1.5 2,767,790 + Pf1N1B4_2442 0.58 -0.0 2,767,790 + Pf1N1B4_2442 0.58 -0.0 2,767,791 - Pf1N1B4_2442 0.58 -0.0 2,767,872 - Pf1N1B4_2442 0.69 -0.0 2,768,290 - -1.3 2,768,299 + -0.0 2,768,300 - -2.0 2,768,388 + Pf1N1B4_2443 0.15 -3.4 2,768,389 - Pf1N1B4_2443 0.16 -5.7 2,768,389 - Pf1N1B4_2443 0.16 -0.0 2,768,532 + +0.7 2,768,532 + Pf1N1B4_2443 0.41 -0.0 2,768,532 + Pf1N1B4_2443 0.41 -1.4 2,768,532 + Pf1N1B4_2443 0.41 -0.0 2,768,532 + Pf1N1B4_2443 0.41 -0.0 2,768,532 + Pf1N1B4_2443 0.41 -2.3 2,768,533 - Pf1N1B4_2443 0.41 -4.5 2,768,533 - Pf1N1B4_2443 0.41 -0.0 2,768,533 - Pf1N1B4_2443 0.41 -0.0 2,768,533 - Pf1N1B4_2443 0.41 -0.0 2,768,590 - Pf1N1B4_2443 0.51 -0.0 2,768,619 + Pf1N1B4_2443 0.56 -0.0 2,768,619 + Pf1N1B4_2443 0.56 -0.0 2,768,620 - Pf1N1B4_2443 0.56 -1.7 2,768,621 + Pf1N1B4_2443 0.56 -1.5 2,768,621 + -1.0 2,768,621 + Pf1N1B4_2443 0.56 -0.0 2,768,621 + Pf1N1B4_2443 0.56 -0.0 2,768,622 - Pf1N1B4_2443 0.57 -0.0 2,768,622 - Pf1N1B4_2443 0.57 -0.0 2,768,622 - Pf1N1B4_2443 0.57 -0.0 2,768,686 - Pf1N1B4_2443 0.68 -0.0 2,768,692 - Pf1N1B4_2443 0.69 -0.0 2,768,769 + Pf1N1B4_2443 0.83 +0.4 2,768,769 + Pf1N1B4_2443 0.83 -0.0 2,768,847 + -0.0 2,768,847 + -0.0 2,768,847 + -0.0 2,768,847 + -0.0 2,768,847 + -1.0 2,768,848 - -0.8 2,768,848 - -0.0 2,768,848 - -0.0 2,768,848 - -0.0 2,768,848 - -2.7 2,768,856 + -0.0 2,768,857 - -0.0 2,768,866 - +0.9 2,768,981 + -0.0 2,768,981 + -0.0 2,768,982 - -0.0 2,768,982 - -0.0 2,768,982 - -0.0 2,768,982 - -0.0 2,768,982 - -0.0
Or see this region's nucleotide sequence