Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2442

Experiment: Nitrate reduction

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2441 and Pf1N1B4_2442 are separated by 15 nucleotidesPf1N1B4_2442 and Pf1N1B4_2443 are separated by 188 nucleotides Pf1N1B4_2441 - Electron transfer flavoprotein, alpha subunit, at 2,766,106 to 2,767,326 _2441 Pf1N1B4_2442 - Electron transfer flavoprotein, beta subunit, at 2,767,342 to 2,768,112 _2442 Pf1N1B4_2443 - acetyltransferase, GNAT family, at 2,768,301 to 2,768,867 _2443 Position (kb) 2767 2768 2769Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 2766.373 kb on + strand, within Pf1N1B4_2441at 2766.373 kb on + strand, within Pf1N1B4_2441at 2766.373 kb on + strand, within Pf1N1B4_2441at 2766.374 kb on - strand, within Pf1N1B4_2441at 2766.374 kb on - strand, within Pf1N1B4_2441at 2766.374 kb on - strand, within Pf1N1B4_2441at 2766.483 kb on - strand, within Pf1N1B4_2441at 2766.483 kb on - strandat 2766.568 kb on + strand, within Pf1N1B4_2441at 2767.120 kb on + strand, within Pf1N1B4_2441at 2767.121 kb on - strand, within Pf1N1B4_2441at 2767.286 kb on + strandat 2767.286 kb on + strandat 2767.342 kb on - strandat 2767.510 kb on + strand, within Pf1N1B4_2442at 2767.511 kb on - strand, within Pf1N1B4_2442at 2767.588 kb on + strand, within Pf1N1B4_2442at 2767.588 kb on + strand, within Pf1N1B4_2442at 2767.589 kb on - strand, within Pf1N1B4_2442at 2767.589 kb on - strand, within Pf1N1B4_2442at 2767.589 kb on - strand, within Pf1N1B4_2442at 2767.790 kb on + strandat 2767.790 kb on + strand, within Pf1N1B4_2442at 2767.790 kb on + strand, within Pf1N1B4_2442at 2767.791 kb on - strand, within Pf1N1B4_2442at 2767.872 kb on - strand, within Pf1N1B4_2442at 2768.290 kb on - strandat 2768.299 kb on + strandat 2768.300 kb on - strandat 2768.388 kb on + strand, within Pf1N1B4_2443at 2768.389 kb on - strand, within Pf1N1B4_2443at 2768.389 kb on - strand, within Pf1N1B4_2443at 2768.532 kb on + strandat 2768.532 kb on + strand, within Pf1N1B4_2443at 2768.532 kb on + strand, within Pf1N1B4_2443at 2768.532 kb on + strand, within Pf1N1B4_2443at 2768.532 kb on + strand, within Pf1N1B4_2443at 2768.532 kb on + strand, within Pf1N1B4_2443at 2768.533 kb on - strand, within Pf1N1B4_2443at 2768.533 kb on - strand, within Pf1N1B4_2443at 2768.533 kb on - strand, within Pf1N1B4_2443at 2768.533 kb on - strand, within Pf1N1B4_2443at 2768.590 kb on - strand, within Pf1N1B4_2443at 2768.619 kb on + strand, within Pf1N1B4_2443at 2768.619 kb on + strand, within Pf1N1B4_2443at 2768.620 kb on - strand, within Pf1N1B4_2443at 2768.621 kb on + strand, within Pf1N1B4_2443at 2768.621 kb on + strandat 2768.621 kb on + strand, within Pf1N1B4_2443at 2768.621 kb on + strand, within Pf1N1B4_2443at 2768.622 kb on - strand, within Pf1N1B4_2443at 2768.622 kb on - strand, within Pf1N1B4_2443at 2768.622 kb on - strand, within Pf1N1B4_2443at 2768.686 kb on - strand, within Pf1N1B4_2443at 2768.692 kb on - strand, within Pf1N1B4_2443at 2768.769 kb on + strand, within Pf1N1B4_2443at 2768.769 kb on + strand, within Pf1N1B4_2443at 2768.847 kb on + strandat 2768.847 kb on + strandat 2768.847 kb on + strandat 2768.847 kb on + strandat 2768.847 kb on + strandat 2768.848 kb on - strandat 2768.848 kb on - strandat 2768.848 kb on - strandat 2768.848 kb on - strandat 2768.848 kb on - strandat 2768.856 kb on + strandat 2768.857 kb on - strandat 2768.866 kb on - strandat 2768.981 kb on + strandat 2768.981 kb on + strandat 2768.982 kb on - strandat 2768.982 kb on - strandat 2768.982 kb on - strandat 2768.982 kb on - strandat 2768.982 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction
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2,766,373 + Pf1N1B4_2441 0.22 -0.0
2,766,373 + Pf1N1B4_2441 0.22 -0.0
2,766,373 + Pf1N1B4_2441 0.22 -0.0
2,766,374 - Pf1N1B4_2441 0.22 -0.0
2,766,374 - Pf1N1B4_2441 0.22 +0.3
2,766,374 - Pf1N1B4_2441 0.22 -1.6
2,766,483 - Pf1N1B4_2441 0.31 -1.1
2,766,483 - -2.9
2,766,568 + Pf1N1B4_2441 0.38 -3.9
2,767,120 + Pf1N1B4_2441 0.83 -0.0
2,767,121 - Pf1N1B4_2441 0.83 -0.0
2,767,286 + -0.0
2,767,286 + -0.0
2,767,342 - -0.0
2,767,510 + Pf1N1B4_2442 0.22 -3.2
2,767,511 - Pf1N1B4_2442 0.22 -4.1
2,767,588 + Pf1N1B4_2442 0.32 -0.0
2,767,588 + Pf1N1B4_2442 0.32 -0.0
2,767,589 - Pf1N1B4_2442 0.32 -0.0
2,767,589 - Pf1N1B4_2442 0.32 -2.5
2,767,589 - Pf1N1B4_2442 0.32 -0.0
2,767,790 + +1.5
2,767,790 + Pf1N1B4_2442 0.58 -0.0
2,767,790 + Pf1N1B4_2442 0.58 -0.0
2,767,791 - Pf1N1B4_2442 0.58 -0.0
2,767,872 - Pf1N1B4_2442 0.69 -0.0
2,768,290 - -1.3
2,768,299 + -0.0
2,768,300 - -2.0
2,768,388 + Pf1N1B4_2443 0.15 -3.4
2,768,389 - Pf1N1B4_2443 0.16 -5.7
2,768,389 - Pf1N1B4_2443 0.16 -0.0
2,768,532 + +0.7
2,768,532 + Pf1N1B4_2443 0.41 -0.0
2,768,532 + Pf1N1B4_2443 0.41 -1.4
2,768,532 + Pf1N1B4_2443 0.41 -0.0
2,768,532 + Pf1N1B4_2443 0.41 -0.0
2,768,532 + Pf1N1B4_2443 0.41 -2.3
2,768,533 - Pf1N1B4_2443 0.41 -4.5
2,768,533 - Pf1N1B4_2443 0.41 -0.0
2,768,533 - Pf1N1B4_2443 0.41 -0.0
2,768,533 - Pf1N1B4_2443 0.41 -0.0
2,768,590 - Pf1N1B4_2443 0.51 -0.0
2,768,619 + Pf1N1B4_2443 0.56 -0.0
2,768,619 + Pf1N1B4_2443 0.56 -0.0
2,768,620 - Pf1N1B4_2443 0.56 -1.7
2,768,621 + Pf1N1B4_2443 0.56 -1.5
2,768,621 + -1.0
2,768,621 + Pf1N1B4_2443 0.56 -0.0
2,768,621 + Pf1N1B4_2443 0.56 -0.0
2,768,622 - Pf1N1B4_2443 0.57 -0.0
2,768,622 - Pf1N1B4_2443 0.57 -0.0
2,768,622 - Pf1N1B4_2443 0.57 -0.0
2,768,686 - Pf1N1B4_2443 0.68 -0.0
2,768,692 - Pf1N1B4_2443 0.69 -0.0
2,768,769 + Pf1N1B4_2443 0.83 +0.4
2,768,769 + Pf1N1B4_2443 0.83 -0.0
2,768,847 + -0.0
2,768,847 + -0.0
2,768,847 + -0.0
2,768,847 + -0.0
2,768,847 + -1.0
2,768,848 - -0.8
2,768,848 - -0.0
2,768,848 - -0.0
2,768,848 - -0.0
2,768,848 - -2.7
2,768,856 + -0.0
2,768,857 - -0.0
2,768,866 - +0.9
2,768,981 + -0.0
2,768,981 + -0.0
2,768,982 - -0.0
2,768,982 - -0.0
2,768,982 - -0.0
2,768,982 - -0.0
2,768,982 - -0.0

Or see this region's nucleotide sequence