Experiment: Nitrate reduction
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2395 and Pf1N1B4_2396 are separated by 18 nucleotides Pf1N1B4_2396 and Pf1N1B4_2397 are separated by 273 nucleotides
Pf1N1B4_2395 - S-adenosylmethionine synthetase (EC 2.5.1.6), at 2,712,971 to 2,714,161
_2395
Pf1N1B4_2396 - Transcriptional regulator, ArsR family / Methyltransferase fusion, at 2,714,180 to 2,715,175
_2396
Pf1N1B4_2397 - Transketolase (EC 2.2.1.1), at 2,715,449 to 2,717,446
_2397
Position (kb)
2714
2715
2716 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2714.243 kb on - strand at 2714.247 kb on - strand at 2714.247 kb on - strand at 2714.247 kb on - strand at 2714.247 kb on - strand at 2714.247 kb on - strand at 2714.253 kb on - strand at 2714.253 kb on - strand at 2714.253 kb on - strand at 2714.253 kb on - strand at 2714.253 kb on - strand at 2714.253 kb on - strand at 2714.253 kb on - strand at 2714.253 kb on - strand at 2714.253 kb on - strand at 2714.253 kb on - strand at 2714.257 kb on - strand at 2714.257 kb on - strand at 2714.257 kb on - strand at 2714.257 kb on - strand at 2714.257 kb on - strand at 2714.257 kb on - strand at 2714.257 kb on - strand at 2714.376 kb on - strand, within Pf1N1B4_2396 at 2714.376 kb on - strand, within Pf1N1B4_2396 at 2714.376 kb on - strand, within Pf1N1B4_2396 at 2714.376 kb on - strand, within Pf1N1B4_2396 at 2714.376 kb on - strand, within Pf1N1B4_2396 at 2714.376 kb on - strand, within Pf1N1B4_2396 at 2714.376 kb on - strand, within Pf1N1B4_2396 at 2714.376 kb on - strand, within Pf1N1B4_2396 at 2714.376 kb on - strand, within Pf1N1B4_2396 at 2714.376 kb on - strand, within Pf1N1B4_2396 at 2714.463 kb on - strand, within Pf1N1B4_2396 at 2714.463 kb on - strand, within Pf1N1B4_2396 at 2714.463 kb on - strand, within Pf1N1B4_2396 at 2714.463 kb on - strand, within Pf1N1B4_2396 at 2714.475 kb on - strand, within Pf1N1B4_2396 at 2714.475 kb on - strand, within Pf1N1B4_2396 at 2714.475 kb on - strand, within Pf1N1B4_2396 at 2714.475 kb on - strand, within Pf1N1B4_2396 at 2714.499 kb on - strand, within Pf1N1B4_2396 at 2714.715 kb on - strand, within Pf1N1B4_2396 at 2715.051 kb on - strand, within Pf1N1B4_2396 at 2715.084 kb on - strand at 2715.084 kb on - strand at 2715.084 kb on - strand at 2715.168 kb on - strand at 2715.176 kb on - strand at 2715.246 kb on + strand at 2715.266 kb on + strand at 2715.266 kb on + strand at 2715.266 kb on + strand at 2715.267 kb on - strand at 2715.272 kb on + strand at 2715.313 kb on - strand at 2715.313 kb on - strand at 2715.313 kb on - strand at 2715.313 kb on - strand at 2715.350 kb on - strand at 2715.350 kb on - strand at 2715.350 kb on - strand at 2715.350 kb on - strand at 2715.350 kb on - strand at 2715.415 kb on + strand at 2715.415 kb on + strand at 2715.415 kb on + strand at 2715.415 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction remove 2,714,243 - -0.0 2,714,247 - -1.7 2,714,247 - -0.0 2,714,247 - -0.0 2,714,247 - -0.6 2,714,247 - -0.0 2,714,253 - -0.0 2,714,253 - -0.0 2,714,253 - -0.0 2,714,253 - -0.0 2,714,253 - -0.0 2,714,253 - -0.0 2,714,253 - -2.2 2,714,253 - -2.8 2,714,253 - -0.0 2,714,253 - -0.0 2,714,257 - -4.3 2,714,257 - -0.0 2,714,257 - -0.6 2,714,257 - -2.2 2,714,257 - -0.0 2,714,257 - -0.0 2,714,257 - -0.0 2,714,376 - Pf1N1B4_2396 0.20 -0.0 2,714,376 - Pf1N1B4_2396 0.20 -0.0 2,714,376 - Pf1N1B4_2396 0.20 -0.6 2,714,376 - Pf1N1B4_2396 0.20 -0.0 2,714,376 - Pf1N1B4_2396 0.20 +0.1 2,714,376 - Pf1N1B4_2396 0.20 -0.0 2,714,376 - Pf1N1B4_2396 0.20 -0.0 2,714,376 - Pf1N1B4_2396 0.20 -0.0 2,714,376 - Pf1N1B4_2396 0.20 -0.0 2,714,376 - Pf1N1B4_2396 0.20 -0.0 2,714,463 - Pf1N1B4_2396 0.28 -0.0 2,714,463 - Pf1N1B4_2396 0.28 -1.6 2,714,463 - Pf1N1B4_2396 0.28 -0.5 2,714,463 - Pf1N1B4_2396 0.28 -0.0 2,714,475 - Pf1N1B4_2396 0.30 -0.0 2,714,475 - Pf1N1B4_2396 0.30 -0.0 2,714,475 - Pf1N1B4_2396 0.30 -4.7 2,714,475 - Pf1N1B4_2396 0.30 -3.6 2,714,499 - Pf1N1B4_2396 0.32 -0.0 2,714,715 - Pf1N1B4_2396 0.54 -0.0 2,715,051 - Pf1N1B4_2396 0.87 -0.0 2,715,084 - -0.0 2,715,084 - -3.0 2,715,084 - -0.0 2,715,168 - -0.0 2,715,176 - -2.2 2,715,246 + -0.0 2,715,266 + -2.7 2,715,266 + -1.3 2,715,266 + -0.0 2,715,267 - -0.0 2,715,272 + -1.9 2,715,313 - +0.6 2,715,313 - -2.5 2,715,313 - -1.7 2,715,313 - -0.0 2,715,350 - -0.0 2,715,350 - -0.0 2,715,350 - -0.0 2,715,350 - -0.0 2,715,350 - -0.0 2,715,415 + -0.0 2,715,415 + -0.0 2,715,415 + -6.4 2,715,415 + +1.5
Or see this region's nucleotide sequence