Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2271

Experiment: Nitrate reduction

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2269 and Pf1N1B4_2270 are separated by 129 nucleotidesPf1N1B4_2270 and Pf1N1B4_2271 are separated by 135 nucleotidesPf1N1B4_2271 and Pf1N1B4_2272 are separated by 63 nucleotides Pf1N1B4_2269 - Purine nucleoside phosphorylase (EC 2.4.2.1), at 2,586,416 to 2,586,901 _2269 Pf1N1B4_2270 - ABC-type amino acid transport/signal transduction systems, periplasmic component/domain, at 2,587,031 to 2,587,618 _2270 Pf1N1B4_2271 - ATPases of the AAA+ class, at 2,587,754 to 2,588,179 _2271 Pf1N1B4_2272 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 2,588,243 to 2,589,472 _2272 Position (kb) 2587 2588 2589Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 2586.767 kb on + strand, within Pf1N1B4_2269at 2586.767 kb on + strand, within Pf1N1B4_2269at 2586.768 kb on - strand, within Pf1N1B4_2269at 2586.768 kb on - strand, within Pf1N1B4_2269at 2586.768 kb on - strand, within Pf1N1B4_2269at 2586.768 kb on - strand, within Pf1N1B4_2269at 2586.768 kb on - strand, within Pf1N1B4_2269at 2586.970 kb on - strandat 2586.977 kb on + strandat 2587.015 kb on - strandat 2587.284 kb on + strand, within Pf1N1B4_2270at 2587.284 kb on + strand, within Pf1N1B4_2270at 2587.284 kb on + strand, within Pf1N1B4_2270at 2587.285 kb on - strand, within Pf1N1B4_2270at 2587.285 kb on - strand, within Pf1N1B4_2270at 2587.332 kb on - strand, within Pf1N1B4_2270at 2587.353 kb on + strand, within Pf1N1B4_2270at 2587.354 kb on - strand, within Pf1N1B4_2270at 2587.354 kb on - strand, within Pf1N1B4_2270at 2587.501 kb on + strand, within Pf1N1B4_2270at 2587.501 kb on + strand, within Pf1N1B4_2270at 2587.501 kb on + strand, within Pf1N1B4_2270at 2587.501 kb on + strand, within Pf1N1B4_2270at 2587.501 kb on + strand, within Pf1N1B4_2270at 2587.501 kb on + strand, within Pf1N1B4_2270at 2587.502 kb on - strand, within Pf1N1B4_2270at 2587.542 kb on - strand, within Pf1N1B4_2270at 2587.542 kb on - strand, within Pf1N1B4_2270at 2587.640 kb on + strandat 2587.640 kb on + strandat 2587.766 kb on - strandat 2587.825 kb on + strand, within Pf1N1B4_2271at 2587.826 kb on - strand, within Pf1N1B4_2271at 2587.826 kb on - strand, within Pf1N1B4_2271at 2587.826 kb on - strand, within Pf1N1B4_2271at 2587.826 kb on - strand, within Pf1N1B4_2271at 2587.826 kb on - strand, within Pf1N1B4_2271at 2588.070 kb on + strand, within Pf1N1B4_2271at 2588.070 kb on + strand, within Pf1N1B4_2271at 2588.070 kb on + strand, within Pf1N1B4_2271at 2588.070 kb on + strand, within Pf1N1B4_2271at 2588.071 kb on - strand, within Pf1N1B4_2271at 2588.071 kb on - strand, within Pf1N1B4_2271at 2588.071 kb on - strand, within Pf1N1B4_2271at 2588.173 kb on + strandat 2588.174 kb on - strandat 2588.174 kb on - strandat 2588.174 kb on - strandat 2588.177 kb on + strandat 2588.177 kb on + strandat 2588.177 kb on + strandat 2588.177 kb on + strandat 2588.177 kb on + strandat 2588.177 kb on + strandat 2588.178 kb on - strandat 2588.178 kb on - strandat 2588.178 kb on - strandat 2588.178 kb on - strandat 2588.178 kb on - strandat 2588.238 kb on - strandat 2588.515 kb on + strand, within Pf1N1B4_2272at 2588.530 kb on - strand, within Pf1N1B4_2272at 2588.678 kb on + strand, within Pf1N1B4_2272at 2588.678 kb on + strand, within Pf1N1B4_2272at 2588.679 kb on - strand, within Pf1N1B4_2272at 2588.679 kb on - strand, within Pf1N1B4_2272at 2588.934 kb on + strand, within Pf1N1B4_2272at 2588.935 kb on - strand, within Pf1N1B4_2272at 2588.935 kb on - strand, within Pf1N1B4_2272at 2588.935 kb on - strand, within Pf1N1B4_2272at 2588.935 kb on - strandat 2588.940 kb on + strand, within Pf1N1B4_2272at 2588.940 kb on + strand, within Pf1N1B4_2272at 2588.941 kb on - strand, within Pf1N1B4_2272at 2588.941 kb on - strand, within Pf1N1B4_2272at 2588.941 kb on - strand, within Pf1N1B4_2272at 2588.941 kb on - strand, within Pf1N1B4_2272at 2588.941 kb on - strand, within Pf1N1B4_2272at 2588.941 kb on - strand, within Pf1N1B4_2272

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction
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2,586,767 + Pf1N1B4_2269 0.72 -0.0
2,586,767 + Pf1N1B4_2269 0.72 -0.0
2,586,768 - Pf1N1B4_2269 0.72 -0.0
2,586,768 - Pf1N1B4_2269 0.72 -1.9
2,586,768 - Pf1N1B4_2269 0.72 -2.3
2,586,768 - Pf1N1B4_2269 0.72 -0.0
2,586,768 - Pf1N1B4_2269 0.72 -0.0
2,586,970 - -0.0
2,586,977 + -2.7
2,587,015 - -1.0
2,587,284 + Pf1N1B4_2270 0.43 -0.0
2,587,284 + Pf1N1B4_2270 0.43 -0.0
2,587,284 + Pf1N1B4_2270 0.43 -0.0
2,587,285 - Pf1N1B4_2270 0.43 -0.0
2,587,285 - Pf1N1B4_2270 0.43 -1.9
2,587,332 - Pf1N1B4_2270 0.51 -0.0
2,587,353 + Pf1N1B4_2270 0.55 -0.0
2,587,354 - Pf1N1B4_2270 0.55 -0.0
2,587,354 - Pf1N1B4_2270 0.55 -0.0
2,587,501 + Pf1N1B4_2270 0.80 -2.2
2,587,501 + Pf1N1B4_2270 0.80 -0.0
2,587,501 + Pf1N1B4_2270 0.80 -0.0
2,587,501 + Pf1N1B4_2270 0.80 -2.6
2,587,501 + Pf1N1B4_2270 0.80 -0.0
2,587,501 + Pf1N1B4_2270 0.80 -0.0
2,587,502 - Pf1N1B4_2270 0.80 -0.0
2,587,542 - Pf1N1B4_2270 0.87 -0.0
2,587,542 - Pf1N1B4_2270 0.87 -0.0
2,587,640 + -0.0
2,587,640 + -0.0
2,587,766 - -0.0
2,587,825 + Pf1N1B4_2271 0.17 -0.0
2,587,826 - Pf1N1B4_2271 0.17 -0.0
2,587,826 - Pf1N1B4_2271 0.17 -0.0
2,587,826 - Pf1N1B4_2271 0.17 -4.3
2,587,826 - Pf1N1B4_2271 0.17 -1.9
2,587,826 - Pf1N1B4_2271 0.17 -0.0
2,588,070 + Pf1N1B4_2271 0.74 -0.0
2,588,070 + Pf1N1B4_2271 0.74 -0.0
2,588,070 + Pf1N1B4_2271 0.74 -4.2
2,588,070 + Pf1N1B4_2271 0.74 -0.0
2,588,071 - Pf1N1B4_2271 0.74 -0.0
2,588,071 - Pf1N1B4_2271 0.74 -1.2
2,588,071 - Pf1N1B4_2271 0.74 -0.0
2,588,173 + -0.0
2,588,174 - -0.0
2,588,174 - -0.0
2,588,174 - -0.0
2,588,177 + -0.0
2,588,177 + -0.0
2,588,177 + -1.9
2,588,177 + -0.0
2,588,177 + -1.2
2,588,177 + -0.0
2,588,178 - -0.0
2,588,178 - -0.7
2,588,178 - -3.1
2,588,178 - -0.0
2,588,178 - -0.0
2,588,238 - +0.1
2,588,515 + Pf1N1B4_2272 0.22 -3.5
2,588,530 - Pf1N1B4_2272 0.23 -0.0
2,588,678 + Pf1N1B4_2272 0.35 -3.0
2,588,678 + Pf1N1B4_2272 0.35 -0.0
2,588,679 - Pf1N1B4_2272 0.35 -0.0
2,588,679 - Pf1N1B4_2272 0.35 -4.7
2,588,934 + Pf1N1B4_2272 0.56 -1.2
2,588,935 - Pf1N1B4_2272 0.56 -0.0
2,588,935 - Pf1N1B4_2272 0.56 -0.0
2,588,935 - Pf1N1B4_2272 0.56 -5.9
2,588,935 - -3.5
2,588,940 + Pf1N1B4_2272 0.57 -5.4
2,588,940 + Pf1N1B4_2272 0.57 -0.0
2,588,941 - Pf1N1B4_2272 0.57 -0.0
2,588,941 - Pf1N1B4_2272 0.57 -0.0
2,588,941 - Pf1N1B4_2272 0.57 -0.0
2,588,941 - Pf1N1B4_2272 0.57 -0.0
2,588,941 - Pf1N1B4_2272 0.57 -0.0
2,588,941 - Pf1N1B4_2272 0.57 -0.0

Or see this region's nucleotide sequence